FastQCFastQC Report
Fri 3 Apr 2015
H2K5YBCXX l01n02 50072-bar8.342000000044d4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2K5YBCXX l01n02 50072-bar8.342000000044d4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13225565
Sequences flagged as poor quality0
Sequence length101
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTA3650.016.9211846-47
TCCGATC8100.013.19552732-33
TCGCCGT5450.011.76822344-45
GATCGGA45900.010.14211894-95
ATCGGAA44900.09.89191994-95
AGATCGG49450.09.84629494-95
GCCGTAT8250.08.63808546-47
AGCGTCG15200.08.59417994-95
CGATCTA6953.6379788E-128.54341434-35
TCGGAAG42600.08.36309394-95
TAGATCG19500.08.28222992-93
GACGTGT13050.08.18958420-21
GTCGCCG9100.08.0921544-45
TCAGACG15350.07.890825718-19
CGTCGTG13950.07.83193294-95
CCGATCT10600.07.6185532-33
CCGTATC10150.07.489262648-49
GCGTCGT17100.07.08368794-95
TGGTCGC10159.094947E-126.78677242-43
CGGAAGA41400.06.769661494-95
Produced by FastQC (version 0.11.2)