##FastQC	0.11.2
>>Basic Statistics	pass
#Measure	Value
Filename	H2K5YBCXX l01n02 12330-bar9.34200000004484.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	14900464
Sequences flagged as poor quality	0
Sequence length	101
%GC	25
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.30153463677373	38.0	38.0	38.0	32.0	38.0
2	36.19825812135783	38.0	38.0	38.0	32.0	38.0
3	36.140185970047646	38.0	38.0	38.0	32.0	38.0
4	36.109175123674	38.0	38.0	38.0	32.0	38.0
5	36.07465814487388	38.0	38.0	38.0	32.0	38.0
6	38.214238160637144	40.0	38.0	40.0	38.0	40.0
7	38.14555029964168	40.0	38.0	40.0	38.0	40.0
8	38.2189565371924	40.0	38.0	40.0	38.0	40.0
9	38.17742903845142	40.0	38.0	40.0	38.0	40.0
10-11	38.17187327186589	40.0	38.0	40.0	38.0	40.0
12-13	38.15132542181237	40.0	38.0	40.0	38.0	40.0
14-15	38.104262759871105	40.0	38.0	40.0	38.0	40.0
16-17	38.10213769181953	40.0	38.0	40.0	38.0	40.0
18-19	38.08450944883327	40.0	38.0	40.0	38.0	40.0
20-21	38.07026073147789	40.0	38.0	40.0	38.0	40.0
22-23	38.046210742162124	40.0	38.0	40.0	38.0	40.0
24-25	37.96012020162594	40.0	38.0	40.0	38.0	40.0
26-27	37.94566820201035	40.0	38.0	40.0	38.0	40.0
28-29	37.80748344481084	40.0	38.0	40.0	32.0	40.0
30-31	37.78280129397312	40.0	38.0	40.0	32.0	40.0
32-33	37.7865322516131	40.0	38.0	40.0	32.0	40.0
34-35	37.657799079277	40.0	38.0	40.0	32.0	40.0
36-37	37.6704792213182	40.0	38.0	40.0	32.0	40.0
38-39	37.63198417847927	40.0	38.0	40.0	32.0	40.0
40-41	37.586070440490985	40.0	38.0	40.0	32.0	40.0
42-43	37.458803195658874	40.0	38.0	40.0	32.0	40.0
44-45	37.34334229457552	40.0	38.0	40.0	32.0	40.0
46-47	37.26884770165546	40.0	38.0	40.0	32.0	40.0
48-49	37.22285544262246	40.0	38.0	40.0	32.0	40.0
50-51	37.13444007515471	40.0	38.0	40.0	32.0	40.0
52-53	37.07763731384472	40.0	38.0	40.0	32.0	40.0
54-55	37.038015695350154	40.0	38.0	40.0	32.0	40.0
56-57	36.95943378004873	40.0	38.0	40.0	32.0	40.0
58-59	36.88669426670204	40.0	38.0	40.0	32.0	40.0
60-61	36.83293959839103	40.0	38.0	40.0	32.0	40.0
62-63	36.78874671285404	40.0	38.0	40.0	32.0	40.0
64-65	36.70172865086617	40.0	38.0	40.0	32.0	40.0
66-67	36.66839220577292	39.0	38.0	40.0	32.0	40.0
68-69	36.582498571856554	38.0	38.0	40.0	27.0	40.0
70-71	36.46205614805015	38.0	38.0	40.0	27.0	40.0
72-73	36.407546738141846	38.0	38.0	40.0	27.0	40.0
74-75	36.28842031362245	38.0	38.0	40.0	27.0	40.0
76-77	36.20906318756248	38.0	38.0	40.0	27.0	40.0
78-79	36.15792323648445	38.0	38.0	40.0	27.0	40.0
80-81	36.061655160537285	38.0	38.0	40.0	27.0	40.0
82-83	35.96604484934161	38.0	38.0	40.0	27.0	40.0
84-85	35.82327895963508	38.0	38.0	40.0	27.0	40.0
86-87	35.7383029145938	38.0	38.0	40.0	27.0	40.0
88-89	35.68383585236003	38.0	38.0	40.0	27.0	40.0
90-91	35.57001785313531	38.0	38.0	40.0	27.0	40.0
92-93	35.502694983189784	38.0	38.0	40.0	27.0	40.0
94-95	35.401048148567725	38.0	38.0	40.0	27.0	40.0
96-97	35.27934754246579	38.0	38.0	40.0	27.0	40.0
98-99	35.16171825253227	38.0	38.0	40.0	24.5	40.0
100-101	33.286750969634234	38.0	32.5	39.0	13.0	40.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
2201	1	0.13214079782478905
2201	2	0.021157454460272618
2201	3	0.06263136154007043
2201	4	0.007091057900396436
2201	5	0.11842611692910765
2201	6	0.10309368660106344
2201	7	0.08927723232353912
2201	8	0.08196472511009745
2201	9	0.07217800493248916
2201	10-11	0.12467390116247401
2201	12-13	0.11273316036410819
2201	14-15	0.1260518596598672
2201	16-17	0.05879224194631405
2201	18-19	0.058281751737531806
2201	20-21	0.11252948699197418
2201	22-23	0.023663656371475383
2201	24-25	-0.00594875241501569
2201	26-27	0.04356819410492818
2201	28-29	-0.004777624392986013
2201	30-31	0.01677577899507554
2201	32-33	0.0485202579947952
2201	34-35	0.0874699508940111
2201	36-37	0.053140798107314424
2201	38-39	-0.015355573477989992
2201	40-41	7.335557156622485E-4
2201	42-43	0.03382292046249802
2201	44-45	0.0278578103081486
2201	46-47	-0.011420880162994251
2201	48-49	-0.016911533664973888
2201	50-51	0.015853614206633893
2201	52-53	-0.0014491838103936061
2201	54-55	-0.05602115134809793
2201	56-57	-0.0015251987948730061
2201	58-59	-0.04454638830417679
2201	60-61	-0.13025126591784186
2201	62-63	-0.03517461625796159
2201	64-65	-0.03775391948000362
2201	66-67	-0.081838053193799
2201	68-69	-0.09616300537876299
2201	70-71	-0.1594848598684706
2201	72-73	-0.11890322700280365
2201	74-75	-0.08528134651665198
2201	76-77	-0.0505755167743942
2201	78-79	-0.1014210772905102
2201	80-81	-0.09369077886699273
2201	82-83	-0.1729404549780682
2201	84-85	-0.11493534601501665
2201	86-87	0.009502672083478103
2201	88-89	-0.07595007731180914
2201	90-91	-0.10046762826152644
2201	92-93	-0.08691241590698695
2201	94-95	-0.10712185639653882
2201	96-97	-0.13378016239590806
2201	98-99	-0.19738353073451975
2201	100-101	-0.024617923256194274
1101	1	0.14314332322754098
1101	2	0.113510920044817
1101	3	0.16271233904868154
1101	4	0.10481087741552386
1101	5	0.052418018687895085
1101	6	0.12156452362634695
1101	7	0.14323471780836172
1101	8	0.15253845243353226
1101	9	0.12672794786868735
1101	10-11	0.16501712412712521
1101	12-13	0.15398194276267674
1101	14-15	0.12346685085796594
1101	16-17	0.07116735209651637
1101	18-19	0.07682391668194555
1101	20-21	0.10988159023057875
1101	22-23	0.022635993347073224
1101	24-25	0.07544497252587945
1101	26-27	0.07690360957924014
1101	28-29	0.12452541954281315
1101	30-31	0.058882968182814466
1101	32-33	0.12093036943315383
1101	34-35	0.0783562748684119
1101	36-37	0.07677023067545008
1101	38-39	0.06772804618037753
1101	40-41	-0.09329939388145903
1101	42-43	-0.15907389357425927
1101	44-45	-0.12467512591482688
1101	46-47	-0.17250556352277613
1101	48-49	-0.09254325125291274
1101	50-51	-0.12895978767964067
1101	52-53	-0.1411521021877462
1101	54-55	-0.17663168576994082
1101	56-57	-0.12487267461707319
1101	58-59	-0.0917350807857602
1101	60-61	-0.2648088989444588
1101	62-63	-0.25565645768477907
1101	64-65	-0.27559531081750777
1101	66-67	-0.3254400261253778
1101	68-69	-0.37980633214052517
1101	70-71	-0.3568546872888305
1101	72-73	-0.36684138177141534
1101	74-75	-0.358082496449029
1101	76-77	-0.3324536327721219
1101	78-79	-0.30652424092663466
1101	80-81	-0.3620977625474424
1101	82-83	-0.38219457971386106
1101	84-85	-0.35858904867714614
1101	86-87	-0.28511876629185906
1101	88-89	-0.32926978566677434
1101	90-91	-0.27088575572144435
1101	92-93	-0.32975717228863743
1101	94-95	-0.37542526069223214
1101	96-97	-0.4511833876986344
1101	98-99	-0.39589671151970407
1101	100-101	-0.08412017840834807
2202	1	0.08261866357095471
2202	2	0.04765414662581691
2202	3	0.07422477078905843
2202	4	0.10095123972256204
2202	5	0.04266716389934544
2202	6	0.08485630014181567
2202	7	0.08390199855475089
2202	8	0.054233944346059104
2202	9	0.07315715401573897
2202	10-11	0.0977326920297088
2202	12-13	0.012162777279236536
2202	14-15	0.0203565793776761
2202	16-17	0.03835664048485654
2202	18-19	0.031097307306900746
2202	20-21	0.10110936503816248
2202	22-23	0.011041110514533159
2202	24-25	-0.010941675012908547
2202	26-27	0.002330157883726258
2202	28-29	-0.005650177198823769
2202	30-31	-0.03628596539504514
2202	32-33	0.04098209322732771
2202	34-35	-0.01611925043506801
2202	36-37	-0.010528696402737125
2202	38-39	0.01223636554968266
2202	40-41	-0.0066240687520107144
2202	42-43	-0.07781450255608036
2202	44-45	-0.06643901808831032
2202	46-47	-0.0678545065050784
2202	48-49	-0.11101428107565425
2202	50-51	-0.07237711374585132
2202	52-53	-0.04967620481696855
2202	54-55	-0.06393556720163929
2202	56-57	-0.08407337225089861
2202	58-59	-0.06156187042787309
2202	60-61	-0.1656490323979014
2202	62-63	-0.11519550281841617
2202	64-65	-0.15627273952275544
2202	66-67	-0.24053228869016152
2202	68-69	-0.22214667401913601
2202	70-71	-0.1680312118981817
2202	72-73	-0.18052205970070645
2202	74-75	-0.12785504217757193
2202	76-77	-0.09065034502239655
2202	78-79	-0.14787281521571316
2202	80-81	-0.11227596161294429
2202	82-83	-0.1654028291173475
2202	84-85	-0.1297418284614693
2202	86-87	-0.11304894278917033
2202	88-89	-0.09414916869148726
2202	90-91	-0.12537220808594185
2202	92-93	-0.09348687414710355
2202	94-95	-0.16394310841948112
2202	96-97	-0.18002700195964394
2202	98-99	-0.1171636960232405
2202	100-101	-0.02689830453271469
1102	1	-0.026263153801707517
1102	2	-0.021814894090503856
1102	3	0.05540627973133638
1102	4	-0.02555545593455122
1102	5	0.004131966786403041
1102	6	-0.010397120321634645
1102	7	0.03341408675732538
1102	8	0.048972225048366624
1102	9	0.00619691536844158
1102	10-11	0.07874627148022739
1102	12-13	0.051390371870560614
1102	14-15	0.0750820084668149
1102	16-17	0.04971066861063633
1102	18-19	0.01862012106358435
1102	20-21	0.058869750265053256
1102	22-23	0.009987658079815276
1102	24-25	-0.08910540299284975
1102	26-27	-0.10092949012877028
1102	28-29	-0.046068406532782546
1102	30-31	-0.018729241264452412
1102	32-33	0.06867382121441779
1102	34-35	-0.0032718917702183603
1102	36-37	-0.038387664445473035
1102	38-39	-0.059542026581695495
1102	40-41	-0.06592584262113377
1102	42-43	-0.1296724644269247
1102	44-45	-0.13795602938869678
1102	46-47	-0.13821686114970788
1102	48-49	-0.12448793113086509
1102	50-51	-0.11185675617975477
1102	52-53	-0.15525522666905545
1102	54-55	-0.09983180408902825
1102	56-57	-0.2299964215351622
1102	58-59	-0.1730311748922091
1102	60-61	-0.26377274569607323
1102	62-63	-0.21195270371968888
1102	64-65	-0.32966934300798556
1102	66-67	-0.25184204049017467
1102	68-69	-0.258962906846115
1102	70-71	-0.22200521039249566
1102	72-73	-0.18509277110888434
1102	74-75	-0.19890969546948867
1102	76-77	-0.20028211528656215
1102	78-79	-0.257021588445113
1102	80-81	-0.2085414052350245
1102	82-83	-0.2215424262377894
1102	84-85	-0.26825244997503006
1102	86-87	-0.15044407928770198
1102	88-89	-0.17175446889536516
1102	90-91	-0.21753646772948798
1102	92-93	-0.26656720863673655
1102	94-95	-0.322121631293669
1102	96-97	-0.31745601370535326
1102	98-99	-0.3116866309373094
1102	100-101	-0.058144354340605275
2203	1	-0.013457418096066931
2203	2	-0.019746993362922183
2203	3	-0.02921693621418342
2203	4	0.002771019568108102
2203	5	-0.018412703831870658
2203	6	-0.045800243917796024
2203	7	-0.014476255062270127
2203	8	0.014545005164215752
2203	9	0.019853195603253937
2203	10-11	0.03575928399170181
2203	12-13	0.02302192548622628
2203	14-15	0.036244774292484294
2203	16-17	0.028611275679999437
2203	18-19	-0.014406402511184524
2203	20-21	0.001351382740672591
2203	22-23	0.031066876161098378
2203	24-25	0.013858177334967081
2203	26-27	0.010437445933376921
2203	28-29	0.03963235299420376
2203	30-31	0.03254697805774498
2203	32-33	0.05808553748151013
2203	34-35	-0.003319935746219471
2203	36-37	0.009127496895594334
2203	38-39	0.008866049400936049
2203	40-41	-0.009614784803609666
2203	42-43	-0.08610018376918305
2203	44-45	-0.10009704537538511
2203	46-47	-0.10786656126348504
2203	48-49	-0.09411176034706159
2203	50-51	-0.12650597638417338
2203	52-53	-0.16332789682465432
2203	54-55	-0.1309039593241721
2203	56-57	-0.1374842606624611
2203	58-59	-0.12606971094840702
2203	60-61	-0.22765460200886167
2203	62-63	-0.21014769752562756
2203	64-65	-0.2041943147820504
2203	66-67	-0.17800074393807108
2203	68-69	-0.27047024911339435
2203	70-71	-0.27077733391587344
2203	72-73	-0.15357766279872465
2203	74-75	-0.17568297496319474
2203	76-77	-0.170126469200639
2203	78-79	-0.26001665708098187
2203	80-81	-0.23292165160820133
2203	82-83	-0.27278948963378724
2203	84-85	-0.29758965579711827
2203	86-87	-0.16805885245137375
2203	88-89	-0.2041911101770424
2203	90-91	-0.25681810089877644
2203	92-93	-0.3538319392741087
2203	94-95	-0.20263073873400828
2203	96-97	-0.3288743315816447
2203	98-99	-0.3531230030495749
2203	100-101	-0.19732982138135924
1103	1	9.488493033202872E-4
1103	2	-0.07167522852916619
1103	3	-0.03549608217559097
1103	4	0.023894041202275673
1103	5	-0.045454044250718084
1103	6	-0.02884911626619413
1103	7	0.028634909634696726
1103	8	0.029906127646128766
1103	9	0.042679003736388665
1103	10-11	0.09556501943745133
1103	12-13	0.08033821333879843
1103	14-15	0.09634448793550376
1103	16-17	0.06978190553235919
1103	18-19	0.019990815514056237
1103	20-21	0.07906992070449093
1103	22-23	0.05363318899875935
1103	24-25	0.02985918787073416
1103	26-27	0.048406532913865874
1103	28-29	0.02832005003962479
1103	30-31	0.01227027257692015
1103	32-33	0.08916511877662003
1103	34-35	0.05315754055966693
1103	36-37	0.05531066467023038
1103	38-39	-0.01667861646227209
1103	40-41	0.010427080093158736
1103	42-43	0.014374858488643838
1103	44-45	0.0014158303789102433
1103	46-47	-0.020822487286203284
1103	48-49	-0.011573637087458621
1103	50-51	-0.05473993270240385
1103	52-53	-0.09565390292405596
1103	54-55	-0.0647578519227352
1103	56-57	-0.09686368237724707
1103	58-59	-0.059739384050935485
1103	60-61	-0.1405515840503213
1103	62-63	-0.024223718254631876
1103	64-65	-0.06997197859676874
1103	66-67	-0.09335408875941198
1103	68-69	-0.11158839463485748
1103	70-71	-0.11397117268543866
1103	72-73	-0.1361187773989201
1103	74-75	-0.11013523194870345
1103	76-77	-0.14496702376770543
1103	78-79	-0.16687796732465188
1103	80-81	-0.1392441321878053
1103	82-83	-0.11020973037884829
1103	84-85	-0.23492519688216618
1103	86-87	-0.12671386083047764
1103	88-89	-0.20487206024414206
1103	90-91	-0.23105617667282274
1103	92-93	-0.2734165115111651
1103	94-95	-0.26709513489118564
1103	96-97	-0.2783246346782704
1103	98-99	-0.25572108518454684
1103	100-101	-0.03842536228366811
2204	1	-0.1330722295948732
2204	2	-0.0769633733088142
2204	3	-0.0424925052224836
2204	4	-0.05804839407969098
2204	5	-0.032902325866039916
2204	6	-0.04755913309189452
2204	7	-0.031789782198167416
2204	8	-0.0514649474497233
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1212	92-93	0.21537345049690515
1212	94-95	0.23301299116445762
1212	96-97	0.3267482805599826
1212	98-99	0.3138925960391674
1212	100-101	-0.08506971855073431
1213	1	-0.12358989508788198
1213	2	-0.07763743631745967
1213	3	-0.09682288536987471
1213	4	-0.08017688430825842
1213	5	-0.0624295004767248
1213	6	-0.07799367357868192
1213	7	-0.04938386307410525
1213	8	-0.11679031653510918
1213	9	-0.1364926279843388
1213	10-11	-0.239297137833141
1213	12-13	-0.13676936438010756
1213	14-15	-0.09066618005822136
1213	16-17	-0.12506832449334837
1213	18-19	-0.09246513669925349
1213	20-21	-0.07768588251725816
1213	22-23	-0.05348498025305304
1213	24-25	-0.1313893741784824
1213	26-27	-0.12259455650292495
1213	28-29	-0.01526051848726695
1213	30-31	-0.15981396873795006
1213	32-33	-0.09056919345823644
1213	34-35	-0.06219754609270467
1213	36-37	-0.10636152174989633
1213	38-39	-0.15481004754049366
1213	40-41	-0.10229443250322134
1213	42-43	-0.10263145710495536
1213	44-45	0.015554025944688021
1213	46-47	-0.12242994466156176
1213	48-49	-0.1446627206961324
1213	50-51	-0.2231641984550592
1213	52-53	-0.11185343679657933
1213	54-55	-0.17040880945233994
1213	56-57	-0.07047818259875527
1213	58-59	0.027591539994801906
1213	60-61	0.11267847540073461
1213	62-63	0.03584375484145852
1213	64-65	0.04388530442397354
1213	66-67	-0.07499586749099763
1213	68-69	0.03452060310061
1213	70-71	-6.67229668081859E-4
1213	72-73	0.023102958551866948
1213	74-75	0.0696558275202932
1213	76-77	0.007306259506265178
1213	78-79	0.12169936041129858
1213	80-81	0.06665721355243903
1213	82-83	0.053706417437389575
1213	84-85	0.11891170588934585
1213	86-87	0.007316707105609055
1213	88-89	0.06941658960359831
1213	90-91	0.1302077836409623
1213	92-93	0.05569272319792873
1213	94-95	-0.06038567708598208
1213	96-97	-0.07825580319781977
1213	98-99	-0.15352390149568862
1213	100-101	-0.8790112032857351
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	20.0
4	74.0
5	120.0
6	86.0
7	119.0
8	121.0
9	122.0
10	202.0
11	295.0
12	343.0
13	436.0
14	1783.0
15	9714.0
16	29822.0
17	44629.0
18	47782.0
19	48187.0
20	54413.0
21	65879.0
22	81142.0
23	96598.0
24	111952.0
25	127548.0
26	143394.0
27	159069.0
28	177025.0
29	198698.0
30	222570.0
31	247456.0
32	281048.0
33	325844.0
34	390430.0
35	496161.0
36	686246.0
37	1118351.0
38	2638812.0
39	7093973.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	11.44440589635409	37.581590971583736	38.775729955231704	12.198273176830474
2	12.933803010447436	41.49213043790859	34.467979096132694	11.106087455511277
3	12.550031639209097	39.10184656708875	36.25830537630251	12.089816417399645
4	12.94540522456796	38.50800424071246	35.69683699541588	12.849753539303698
5	13.387241357413004	37.59507721703187	36.65055122801737	12.36713019753775
6	13.287245074317317	38.91799757701046	34.84456958376261	12.950187764909607
7	13.292838585862151	37.92948426281775	36.37007918779436	12.407597963525733
8	12.794552585755714	39.435515847244766	35.289791260184415	12.4801403068151
9	12.932816161991479	37.93425079767422	36.494796081387435	12.63813695894687
10-11	12.77010997100812	38.624717373797665	36.01427686017811	12.590895795016113
12-13	12.664202079790929	38.395432118508246	36.31142596280231	12.628939838898512
14-15	12.614607274624534	38.3388013845168	36.447975837521184	12.598615503337477
16-17	12.737536824715196	38.04044685409441	36.70904774483547	12.512968576354917
18-19	12.694342596665509	37.933151419876545	36.68983408774441	12.682671895713543
20-21	12.856567820443876	37.97498299820625	36.44869445286442	12.71975472848545
22-23	12.927633688030992	37.82953431203883	36.46265201148451	12.780179988445667
24-25	12.859423423885563	37.90718406052664	36.44155543668225	12.791837078905546
26-27	12.961857895032416	37.90378961193452	36.36383166782247	12.7705208252106
28-29	12.9487814919915	37.91018537649403	36.31562896369459	12.825404167819881
30-31	12.901673158360857	37.752989749143744	36.48582693396198	12.859510158533416
32-33	12.860924108729218	37.94758584387073	36.33877731411147	12.852712733288584
34-35	13.003067823818743	37.92592580658176	36.26915728392163	12.801849085677869
36-37	12.827365698507942	37.7742798226541	36.48689865827748	12.911455820560475
38-39	12.849072173531784	37.83435089747748	36.42618446682123	12.890392462169508
40-41	13.024565016354714	37.89125971932303	36.33530875248516	12.748866511837093
42-43	12.908582853682004	37.82073904444452	36.50916038562688	12.761517716246596
44-45	12.944531226865125	37.93720360919668	36.38995993161293	12.728305232325265
46-47	12.932105809166938	37.868453137863014	36.397480709821366	12.80196034314868
48-49	12.775931157013943	37.82755228314637	36.43682127817464	12.959695281665057
50-51	12.89764754019572	37.92920122648515	36.44760066921043	12.725550564108701
52-53	12.782666687120967	38.02375019339551	36.48895271495401	12.70463040452952
54-55	12.764529992160483	37.96212569461759	36.458892615534864	12.814451697687069
56-57	12.805768146893183	37.9410006307526	36.54974637300473	12.703484849349492
58-59	12.802010240308542	38.10944085987363	36.410995501341155	12.677553398476679
60-61	12.747934054214996	38.072158769635024	36.456228903956536	12.723678272193444
62-63	12.740849867523405	38.101070906320494	36.45351084374676	12.704568382409336
64-65	12.71909488852244	38.14261945841715	36.49106672716246	12.64721892589794
66-67	12.74752614417248	38.09972439220151	36.465180527689895	12.687568935936117
68-69	12.7409727156541	38.088762115289995	36.480480876446244	12.689784292609662
70-71	12.766350131208979	38.08852655840056	36.48507237499547	12.66005093539499
72-73	12.72118187128098	38.070113246339915	36.52128420212903	12.687420680250069
74-75	12.776902378970432	38.08274044332308	36.494461960812245	12.64589521689424
76-77	12.740110363806053	38.12105992813348	36.50745369600452	12.631376012055947
78-79	12.731041540222893	38.05071204775927	36.555440453327556	12.662805958690285
80-81	12.760949437368124	38.10858018269184	36.50608815582634	12.624382224113702
82-83	12.742688124219367	38.12728592367673	36.53608683200876	12.59393912009514
84-85	12.75525984619674	38.07003146197026	36.54489386306095	12.629814828772048
86-87	12.80564476226945	38.16450888590187	36.43482634586761	12.595020005961066
88-89	12.782149504666563	38.14470680475739	36.483973566370544	12.589170124205506
90-91	12.782176136996934	38.09523553733991	36.48040243923729	12.642185886425866
92-93	12.80074808256505	38.17753142355354	36.449908693733704	12.5718118001477
94-95	12.822968778541878	38.18775001655964	36.42814105300908	12.561140151889408
96-97	12.877623877418806	38.134498763938595	36.41205041690885	12.575826941733753
98-99	12.961019738658832	38.18789883901677	36.293823841023624	12.557257581300771
100-101	12.97026887020713	38.37586497965661	36.168855135466856	12.485011014669405
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	42423.0
1	44952.0
2	53327.5
3	66732.5
4	84020.0
5	104950.0
6	129158.5
7	156319.0
8	187513.0
9	223602.5
10	260984.0
11	298812.5
12	338328.0
13	376259.5
14	411542.0
15	445559.5
16	475996.0
17	502654.0
18	523494.0
19	536735.0
20	543479.0
21	542226.0
22	534369.5
23	519890.5
24	497472.5
25	470957.0
26	443022.5
27	414362.5
28	387866.5
29	363754.5
30	342659.5
31	325201.0
32	309023.5
33	290466.0
34	268001.0
35	246054.0
36	227775.5
37	213224.5
38	201434.5
39	189560.5
40	179326.0
41	172434.0
42	164460.5
43	154897.5
44	146625.0
45	139427.5
46	133072.5
47	127226.0
48	117537.5
49	104391.5
50	92210.5
51	81755.0
52	72600.5
53	65284.0
54	59306.0
55	53634.0
56	48511.5
57	44281.5
58	40350.5
59	35890.0
60	31890.5
61	28263.5
62	24895.5
63	22246.5
64	20350.5
65	18823.0
66	17527.0
67	16415.0
68	15264.5
69	14188.5
70	13173.0
71	12073.5
72	10711.5
73	9361.5
74	8095.0
75	6779.5
76	5606.0
77	4519.0
78	3526.5
79	2682.5
80	2021.0
81	1535.5
82	1147.0
83	864.0
84	625.0
85	476.0
86	351.0
87	248.0
88	184.0
89	119.5
90	80.5
91	65.0
92	37.0
93	23.0
94	21.0
95	14.0
96	11.0
97	10.0
98	9.0
99	9.0
100	26.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.028240731295347585
2	0.02867695932153522
3	0.029462169768672976
4	0.03890482873553468
5	0.022563055754505363
6	0.010160757409970588
7	0.010583563035352456
8	0.018972563538960936
9	0.008127263687895892
10-11	0.012147272729225077
12-13	0.014036475642637705
14-15	0.03559285133671005
16-17	0.026032746362797825
18-19	0.02809644048668551
20-21	0.013664004020277488
22-23	0.03771023506382083
24-25	0.046461640389185196
26-27	0.0354653385290552
28-29	0.03695522501849607
30-31	0.0474750316500211
32-33	0.04356575741533955
34-35	0.0284353561070313
36-37	0.03845517830854127
38-39	0.04734080764196336
40-41	0.028861517332614606
42-43	0.03827733149786476
44-45	0.05486406329359945
46-47	0.04526704671747135
48-49	0.05736398544367477
50-51	0.053612424418461056
52-53	0.03670691060358926
54-55	0.027770947267145504
56-57	0.03730756303964762
58-59	0.02775416926613829
60-61	0.033868072833168145
62-63	0.029774240587407214
64-65	0.03129768307886251
66-67	0.03248556555017347
68-69	0.03687469061366143
70-71	0.03834444350189363
72-73	0.031290971878459625
74-75	0.03163324309900685
76-77	0.039643060779852225
78-79	0.033619758418261335
80-81	0.037586077856367424
82-83	0.03966654998126233
84-85	0.047558921655057186
86-87	0.048005216481849154
88-89	0.04807232848587802
90-91	0.04888438373462733
92-93	0.0500353546037224
94-95	0.04916289855134712
96-97	0.053689603223094254
98-99	0.05225005073667505
100-101	0.055558672535298226
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	1.4900464E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	88.12277900273907
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.35937410788915	79.62719155335338
2	8.263693138064204	14.56439208304166
3	0.9774607087481216	2.584096620626142
4	0.18066635777702497	0.6368328607845823
5	0.07937285598839872	0.3497278323540947
6	0.014177538837509196	0.07496184730683438
7	0.024310892700886686	0.14996403973876776
8	0.005319438749151078	0.037501098024803774
9	0.007882770335580035	0.06251864653905
>10	0.08642525450928742	1.6323932305999624
>50	9.950525179591649E-4	0.05068392037944759
>100	3.063989004366759E-4	0.03898795338447697
>500	0.0	0.0
>1k	0.0	0.0
>5k	7.862386774078212E-6	0.044890099560053444
>10k+	7.6225955048479895E-6	0.14585821430678933
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG	21714	0.14572700554828358	Illumina Single End PCR Primer 1 (100% over 50bp)
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA Adapter	Nextera Transposase Sequence
1	6.711200402886782E-5	0.0	1.3422400805773565E-5
2	7.382320443175461E-5	0.0	2.684480161154713E-5
3	8.724560523752817E-5	6.711200402886782E-6	3.355600201443391E-5
4	1.0066800604330174E-4	1.3422400805773565E-5	3.355600201443391E-5
5	1.140904068490753E-4	2.013360120866035E-5	4.697840282020748E-5
6	1.2080160725196209E-4	2.684480161154713E-5	4.697840282020748E-5
7	1.2080160725196209E-4	2.684480161154713E-5	5.368960322309426E-5
8	1.4093520846062243E-4	2.684480161154713E-5	7.382320443175461E-5
9	1.4093520846062243E-4	2.684480161154713E-5	7.382320443175461E-5
10-11	2.1140281269093367E-4	2.684480161154713E-5	8.724560523752818E-5
12-13	2.852260171226883E-4	2.684480161154713E-5	1.1073480664763191E-4
14-15	3.590492215544429E-4	2.684480161154713E-5	1.174460070505187E-4
16-17	4.999844300150653E-4	2.684480161154713E-5	1.2415720745340548E-4
18-19	6.644088398857916E-4	3.355600201443391E-5	1.2751280765484888E-4
20-21	9.395680564041496E-4	3.355600201443391E-5	1.3086840785629227E-4
22-23	0.0013187508791672527	4.697840282020748E-5	1.3757960825917903E-4
24-25	0.0017885349073693275	5.368960322309426E-5	1.4093520846062243E-4
26-27	0.0023254309396002703	5.368960322309426E-5	1.4764640886350921E-4
28-29	0.002892527373644203	5.704520342453765E-5	1.6778001007216958E-4
30-31	0.0034327790060765893	6.0400803625981046E-5	1.7449121047505634E-4
32-33	0.0039998754401205225	6.711200402886782E-5	1.946248116837167E-4
34-35	0.004570327474365899	7.046760423031121E-5	2.1140281269093367E-4
36-37	0.005063600703978077	7.382320443175461E-5	2.2146961329526382E-4
38-39	0.005479695128957058	7.7178804633198E-5	2.382476143024808E-4
40-41	0.005858877951720161	8.05344048346414E-5	2.583812155111412E-4
42-43	0.006224638373677491	8.05344048346414E-5	2.684480161154713E-4
44-45	0.006563553994023273	8.05344048346414E-5	2.7180361631691464E-4
46-47	0.006905825214570499	8.05344048346414E-5	2.7515921651835807E-4
48-49	0.007298430438139375	8.724560523752817E-5	2.7515921651835807E-4
50-51	0.007647412859089488	9.060120543897156E-5	2.9529281772701843E-4
52-53	0.007946061277017951	9.395680564041496E-5	3.08715218532792E-4
54-55	0.008389000503608478	1.0066800604330174E-4	3.1207081873423537E-4
56-57	0.008922540935637976	1.0066800604330174E-4	3.187820191371222E-4
58-59	0.009593660975926656	1.0066800604330174E-4	3.221376193385656E-4
60-61	0.010338604220647089	1.0066800604330174E-4	3.2884881974145236E-4
62-63	0.011325150679871446	1.0066800604330174E-4	3.3556002014433915E-4
64-65	0.012576789555009831	1.0066800604330174E-4	3.3556002014433915E-4
66-67	0.014321701659760395	1.0066800604330174E-4	3.4227122054722594E-4
68-69	0.016965914618497786	1.0066800604330174E-4	3.4898242095011267E-4
70-71	0.020855055251970678	1.0066800604330174E-4	3.5569362135299946E-4
72-73	0.026559575594424442	1.0066800604330174E-4	3.6576042195732967E-4
74-75	0.034643216479701575	1.1073480664763191E-4	3.825384229645466E-4
76-77	0.04577709794809075	1.140904068490753E-4	3.892496233674334E-4
78-79	0.061675931702529524	1.140904068490753E-4	4.0267202417320697E-4
80-81	0.08419872025461758	1.140904068490753E-4	4.0938322457609376E-4
82-83	0.11597960976248793	1.140904068490753E-4	4.0938322457609376E-4
84-85	0.15889102513854603	1.140904068490753E-4	4.194500251804239E-4
86-87	0.21642950179269588	1.2080160725196209E-4	4.228056253818673E-4
88-89	0.29101442747017814	1.2080160725196209E-4	4.228056253818673E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCCGTA	2610	0.0	41.227844	46-47
GAGCGTC	6225	0.0	38.148643	9
GCCGTAT	3070	0.0	35.282505	46-47
TCGCCGT	3145	0.0	33.98788	44-45
TCGGTGG	3655	0.0	33.274467	38-39
TGGTCGC	3455	0.0	33.206734	42-43
AGAGCGT	7205	0.0	33.15488	8
CTCGGTG	3660	0.0	33.097378	36-37
GTGGTCG	3655	0.0	31.974524	40-41
TCTCGGT	3800	0.0	31.752989	36-37
CCGTATC	3435	0.0	31.464527	48-49
GGTCGCC	3725	0.0	30.67227	42-43
GTCGCCG	3750	0.0	30.278116	44-45
AAGAGCG	8335	0.0	29.626818	7
CGGTGGT	4145	0.0	29.226324	38-39
GATCTCG	4515	0.0	27.512835	34-35
ATCTCGG	5135	0.0	24.05217	34-35
CGTGTAG	4990	0.0	24.033276	14-15
CGTCGTG	5050	0.0	23.230375	12-13
CGGAAGA	10810	0.0	22.8907	4
>>END_MODULE