FastQCFastQC Report
Fri 3 Apr 2015
H2K5YBCXX l01n02 12330-bar9.34200000004484.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2K5YBCXX l01n02 12330-bar9.34200000004484.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14900464
Sequences flagged as poor quality0
Sequence length101
%GC25

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG217140.14572700554828358Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTA26100.041.22784446-47
GAGCGTC62250.038.1486439
GCCGTAT30700.035.28250546-47
TCGCCGT31450.033.9878844-45
TCGGTGG36550.033.27446738-39
TGGTCGC34550.033.20673442-43
AGAGCGT72050.033.154888
CTCGGTG36600.033.09737836-37
GTGGTCG36550.031.97452440-41
TCTCGGT38000.031.75298936-37
CCGTATC34350.031.46452748-49
GGTCGCC37250.030.6722742-43
GTCGCCG37500.030.27811644-45
AAGAGCG83350.029.6268187
CGGTGGT41450.029.22632438-39
GATCTCG45150.027.51283534-35
ATCTCGG51350.024.0521734-35
CGTGTAG49900.024.03327614-15
CGTCGTG50500.023.23037512-13
CGGAAGA108100.022.89074
Produced by FastQC (version 0.11.2)