##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename H2K5YBCXX l01n02 10868-bar4.342000000043ff.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 13185749 Sequences flagged as poor quality 0 Sequence length 101 %GC 40 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 35.39183416884396 38.0 32.0 38.0 32.0 38.0 2 35.70781386783565 38.0 32.0 38.0 32.0 38.0 3 35.5464986478963 38.0 32.0 38.0 32.0 38.0 4 35.82682189688276 38.0 38.0 38.0 32.0 38.0 5 35.722406819665686 38.0 32.0 38.0 32.0 38.0 6 37.98371689010613 40.0 38.0 40.0 38.0 40.0 7 38.0599225724682 40.0 38.0 40.0 38.0 40.0 8 38.14484220805356 40.0 38.0 40.0 38.0 40.0 9 38.144657690662854 40.0 38.0 40.0 38.0 40.0 10-11 38.11892832178134 40.0 38.0 40.0 38.0 40.0 12-13 38.12149400841773 40.0 38.0 40.0 38.0 40.0 14-15 38.089166493310316 40.0 38.0 40.0 38.0 40.0 16-17 38.0917348722473 40.0 38.0 40.0 38.0 40.0 18-19 38.079319346970735 40.0 38.0 40.0 38.0 40.0 20-21 38.07186649010231 40.0 38.0 40.0 38.0 40.0 22-23 38.06485820411112 40.0 38.0 40.0 38.0 40.0 24-25 38.006355725412334 40.0 38.0 40.0 38.0 40.0 26-27 38.00587660966396 40.0 38.0 40.0 38.0 40.0 28-29 37.91381869926388 40.0 38.0 40.0 38.0 40.0 30-31 37.907788818064105 40.0 38.0 40.0 38.0 40.0 32-33 37.89788926666206 40.0 38.0 40.0 38.0 40.0 34-35 37.85649632038347 40.0 38.0 40.0 32.0 40.0 36-37 37.86610650635015 40.0 38.0 40.0 35.0 40.0 38-39 37.848193265320006 40.0 38.0 40.0 35.0 40.0 40-41 37.80021241114176 40.0 38.0 40.0 32.0 40.0 42-43 37.68957622354255 40.0 38.0 40.0 32.0 40.0 44-45 37.58118124347733 40.0 38.0 40.0 32.0 40.0 46-47 37.524567698050376 40.0 38.0 40.0 32.0 40.0 48-49 37.49992764916122 40.0 38.0 40.0 32.0 40.0 50-51 37.427397905117104 40.0 38.0 40.0 32.0 40.0 52-53 37.391045248927455 40.0 38.0 40.0 32.0 40.0 54-55 37.36812774154885 40.0 38.0 40.0 32.0 40.0 56-57 37.30816903158099 40.0 38.0 40.0 32.0 40.0 58-59 37.24928178141416 40.0 38.0 40.0 32.0 40.0 60-61 37.21291710467111 40.0 38.0 40.0 32.0 40.0 62-63 37.191666662242696 40.0 38.0 40.0 32.0 40.0 64-65 37.117556613583346 38.0 38.0 40.0 32.0 40.0 66-67 37.10902039011967 38.0 38.0 40.0 32.0 40.0 68-69 37.055870697978555 38.0 38.0 40.0 32.0 40.0 70-71 36.95055832626573 38.0 38.0 40.0 32.0 40.0 72-73 36.91600716045785 38.0 38.0 40.0 32.0 40.0 74-75 36.811604065874455 38.0 38.0 40.0 32.0 40.0 76-77 36.747810761451625 38.0 38.0 40.0 32.0 40.0 78-79 36.71561759593634 38.0 38.0 40.0 32.0 40.0 80-81 36.632497971863415 38.0 38.0 40.0 32.0 40.0 82-83 36.55126523339706 38.0 38.0 40.0 32.0 40.0 84-85 36.421359491978805 38.0 38.0 40.0 32.0 40.0 86-87 36.34261497014694 38.0 38.0 40.0 27.0 40.0 88-89 36.303717066053665 38.0 38.0 40.0 27.0 40.0 90-91 36.20177693356669 38.0 38.0 40.0 27.0 40.0 92-93 36.143380212986 38.0 38.0 40.0 27.0 40.0 94-95 36.05439531724743 38.0 38.0 40.0 27.0 40.0 96-97 35.94428166348381 38.0 38.0 40.0 27.0 40.0 98-99 35.838926859596675 38.0 38.0 40.0 27.0 40.0 100-101 33.88874329399111 38.0 32.5 39.0 20.0 40.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2201 1 -0.02333477815000151 2201 2 -0.13586431565811807 2201 3 -0.1454994441588937 2201 4 -0.0047214937908819365 2201 5 0.09028411439853556 2201 6 0.048613206548957066 2201 7 0.08542034375442142 2201 8 0.13326780733765275 2201 9 0.09658776605056119 2201 10-11 0.16727327092814193 2201 12-13 0.16752623225737295 2201 14-15 0.1569265519770937 2201 16-17 0.033214571659740955 2201 18-19 0.05898910118310852 2201 20-21 0.11436230689970017 2201 22-23 0.08112380548926978 2201 24-25 0.08019213203427711 2201 26-27 0.052258416858236956 2201 28-29 0.05526673761343659 2201 30-31 0.09821626265319594 2201 32-33 0.1274537540426124 2201 34-35 0.14874267546004205 2201 36-37 0.1726677548977804 2201 38-39 0.05792314688202538 2201 40-41 0.09482215302791275 2201 42-43 0.08032060858636214 2201 44-45 0.06838909134093285 2201 46-47 0.07193318770649171 2201 48-49 -0.007743091825574311 2201 50-51 0.04503034794257843 2201 52-53 -0.029883610430587737 2201 54-55 0.005996193626998547 2201 56-57 0.003025170456169235 2201 58-59 0.04612663149922014 2201 60-61 -0.06591343929999738 2201 62-63 0.04820430426073585 2201 64-65 0.01778682807491805 2201 66-67 -0.09974524330779388 2201 68-69 -0.032028496688759844 2201 70-71 -0.0906010250984508 2201 72-73 -0.0695636934862165 2201 74-75 -0.05214860181505543 2201 76-77 0.025662730664556932 2201 78-79 -0.02934375685475743 2201 80-81 -0.051166891135771664 2201 82-83 -0.10756822547527634 2201 84-85 -0.0026586253742379995 2201 86-87 0.0596287872558392 2201 88-89 -0.025600586187060514 2201 90-91 -0.11624440369659794 2201 92-93 -0.07465172494975292 2201 94-95 -0.0804189581816317 2201 96-97 -0.0844077784525723 2201 98-99 -0.14467619208976856 2201 100-101 -0.02565488247685721 1101 1 -0.09653306703878428 1101 2 -0.019718254890015885 1101 3 0.006370612741541493 1101 4 0.02916927085431098 1101 5 0.02073047773388481 1101 6 0.11185351196388638 1101 7 0.1402122531711072 1101 8 0.14504001826796298 1101 9 0.18252181941493006 1101 10-11 0.21489584627497038 1101 12-13 0.2313951110172212 1101 14-15 0.15675984473622862 1101 16-17 0.13204198535112255 1101 18-19 0.16021044403478868 1101 20-21 0.19952168157789885 1101 22-23 0.07710925625287501 1101 24-25 0.15328947225798828 1101 26-27 0.1360074937714444 1101 28-29 0.23157290761326976 1101 30-31 0.17162456038783347 1101 32-33 0.1563695971763721 1101 34-35 0.1292534745478946 1101 36-37 0.15292370514385567 1101 38-39 0.14900514813627552 1101 40-41 0.1089826539593659 1101 42-43 0.05490510471361176 1101 44-45 0.061530132861925324 1101 46-47 -0.0014792943871313469 1101 48-49 0.08804537770723186 1101 50-51 0.05965894409489181 1101 52-53 0.031984387598043895 1101 54-55 0.04756206447638078 1101 56-57 0.10706912466041985 1101 58-59 0.11129906587848382 1101 60-61 0.049681041614419996 1101 62-63 0.1431573728730129 1101 64-65 0.10208864707443865 1101 66-67 0.014598418091956944 1101 68-69 0.054062443613354105 1101 70-71 0.05962101164951861 1101 72-73 -0.03260304807525927 1101 74-75 0.04613857624903517 1101 76-77 0.06668851056292624 1101 78-79 0.07314751563819755 1101 80-81 0.03390873366980429 1101 82-83 0.02947826632809125 1101 84-85 0.1078875317238186 1101 86-87 0.20550680834011814 1101 88-89 0.1016895815605352 1101 90-91 0.09593411025887377 1101 92-93 -5.058097645473936E-4 1101 94-95 0.009787533069903986 1101 96-97 0.03328433303152423 1101 98-99 0.1393125706285261 1101 100-101 0.2569490197781974 2202 1 -0.22556014155055948 2202 2 -0.0998948179709842 2202 3 -0.07371118436237367 2202 4 0.032838575387124536 2202 5 -0.04111646995628604 2202 6 0.010879724062647256 2202 7 0.1051810933506303 2202 8 0.12246866637801901 2202 9 0.15500558736700754 2202 10-11 0.16801993386827974 2202 12-13 0.11744094166231633 2202 14-15 0.11211050655652599 2202 16-17 0.11452228518101037 2202 18-19 0.14299358296526066 2202 20-21 0.17107668612369054 2202 22-23 0.08852933791641959 2202 24-25 0.12112921006169586 2202 26-27 0.08733287481378937 2202 28-29 0.08616602413663088 2202 30-31 0.07856522338879302 2202 32-33 0.15089405596247474 2202 34-35 0.1107613220090542 2202 36-37 0.060497396346853805 2202 38-39 0.07915125681366675 2202 40-41 0.1067275931081042 2202 42-43 0.03126894980263728 2202 44-45 0.05544791890137901 2202 46-47 0.006116475725661985 2202 48-49 0.03552605901636241 2202 50-51 0.021074912602472295 2202 52-53 0.06919438346216111 2202 54-55 0.06745418186514485 2202 56-57 0.07495439843961549 2202 58-59 0.04603585444280611 2202 60-61 0.005885875929536155 2202 62-63 0.025687201068116394 2202 64-65 -0.024733449055204915 2202 66-67 -0.09342172457984788 2202 68-69 -0.028432689152076307 2202 70-71 -0.014733482154532851 2202 72-73 -0.012345222103391507 2202 74-75 0.06258160989123951 2202 76-77 0.024166789334309158 2202 78-79 0.013082367225720759 2202 80-81 0.00429393960678226 2202 82-83 -0.003650967327729404 2202 84-85 -0.005025064351052322 2202 86-87 0.030570377556436767 2202 88-89 0.06509492519660398 2202 90-91 0.06314678713346211 2202 92-93 0.08739446460213429 2202 94-95 0.0487357813272169 2202 96-97 0.02943274251885697 2202 98-99 0.05622980355860818 2202 100-101 0.18623015803834875 1102 1 -0.3580301950592002 1102 2 -0.18017146896286818 1102 3 -0.14442749609808203 1102 4 -0.07317315255227896 1102 5 -0.16670221786751682 1102 6 -0.15417633475304626 1102 7 -0.07578533447284741 1102 8 0.02537837963519962 1102 9 -0.01811552391363591 1102 10-11 0.08049613813268763 1102 12-13 0.04693692754303669 1102 14-15 0.07831773787592056 1102 16-17 0.04587082778376583 1102 18-19 0.005553116598193242 1102 20-21 0.08109952435673051 1102 22-23 0.04347544718680041 1102 24-25 -0.04735701127324177 1102 26-27 -0.04153890490517398 1102 28-29 0.018962946245949297 1102 30-31 0.019227603427125928 1102 32-33 0.039825192014255606 1102 34-35 -0.003576197209085308 1102 36-37 0.04182409196374692 1102 38-39 0.03782487494633102 1102 40-41 -0.010481078441152647 1102 42-43 -0.10042048704188744 1102 44-45 -0.14132010478438417 1102 46-47 -0.11987100070260936 1102 48-49 -0.08134568597294844 1102 50-51 -0.0758107490147566 1102 52-53 -0.08969287416781668 1102 54-55 -0.0639283480277868 1102 56-57 -0.1855423440259898 1102 58-59 -0.16758370932337385 1102 60-61 -0.1876576685867022 1102 62-63 -0.13583978859573875 1102 64-65 -0.1992810259674087 1102 66-67 -0.14978710033769005 1102 68-69 -0.15600225895042286 1102 70-71 -0.13280612857047203 1102 72-73 -0.07064180127343889 1102 74-75 -0.12378745899561494 1102 76-77 -0.09389918865961278 1102 78-79 -0.12500681102220312 1102 80-81 -0.1121714048718161 1102 82-83 -0.14716385744750227 1102 84-85 -0.18106751969413182 1102 86-87 -0.09134090296434039 1102 88-89 -0.14412554818332524 1102 90-91 -0.07159707533712378 1102 92-93 -0.1591921002844643 1102 94-95 -0.2541379766063656 1102 96-97 -0.20975734214764685 1102 98-99 -0.13896763256602185 1102 100-101 0.12465831664994909 2203 1 -0.3604571754981265 2203 2 -0.07501405346381773 2203 3 -0.13155660779775502 2203 4 -0.09684154543423062 2203 5 -0.09344299052913385 2203 6 -0.18266048160711534 2203 7 -0.07770736747891505 2203 8 -0.03384936014996498 2203 9 0.050446791128678115 2203 10-11 0.07921086326606996 2203 12-13 0.08243196081112103 2203 14-15 0.023928221794164983 2203 16-17 0.07781221486390422 2203 18-19 0.014100091842820461 2203 20-21 0.020544096014212698 2203 22-23 0.03399856410390356 2203 24-25 0.059077873578615936 2203 26-27 0.05937586729488231 2203 28-29 0.05223895429091385 2203 30-31 0.02894403088255615 2203 32-33 0.0726196932632277 2203 34-35 0.015512434118036822 2203 36-37 -0.005194049680305568 2203 38-39 0.019162634161425274 2203 40-41 0.03678065703120126 2203 42-43 -0.050893311390844076 2203 44-45 -0.06480004645510462 2203 46-47 -0.093197072550538 2203 48-49 -0.03165887502090925 2203 50-51 -0.07978664132466662 2203 52-53 -0.07779117782060041 2203 54-55 -0.05321669701103815 2203 56-57 -0.05555273169503039 2203 58-59 -0.10740322486712728 2203 60-61 -0.17782672388280218 2203 62-63 -0.14135043598130892 2203 64-65 -0.1658155892856783 2203 66-67 -0.11587084172798967 2203 68-69 -0.12372342336480813 2203 70-71 -0.1092489518580706 2203 72-73 -0.08102793387544693 2203 74-75 -0.10185447831351979 2203 76-77 -0.06655054350390088 2203 78-79 -0.09489487649592121 2203 80-81 -0.11279033945289996 2203 82-83 -0.1155383137435706 2203 84-85 -0.15610713923204145 2203 86-87 -0.04693587782133335 2203 88-89 -0.05728898039408392 2203 90-91 -0.1113161913080205 2203 92-93 -0.2259243873164749 2203 94-95 -0.159798226507597 2203 96-97 -0.20176587087695452 2203 98-99 -0.1987086681545378 2203 100-101 0.048782399594557546 1103 1 -0.28022904021369044 1103 2 -0.2597584747861106 1103 3 -0.19738146659357625 1103 4 -0.12906792941034695 1103 5 -0.22845738401833415 1103 6 -0.24352062841551714 1103 7 -0.14623092599940435 1103 8 -0.1074912838063895 1103 9 -0.036579552174046626 1103 10-11 0.050990941718566773 1103 12-13 0.022622587582574738 1103 14-15 0.042116523565951525 1103 16-17 0.032969768034995184 1103 18-19 -0.06078387479399794 1103 20-21 0.00958287645661926 1103 22-23 -0.009613295850265047 1103 24-25 -0.02118826662287887 1103 26-27 -0.032941528007100374 1103 28-29 -0.06537992168132689 1103 30-31 -0.013669375784502336 1103 32-33 0.007429789463095915 1103 34-35 -0.04031972168709075 1103 36-37 -0.030289857241392326 1103 38-39 -0.10960729977497152 1103 40-41 -0.106938224149701 1103 42-43 -0.12450630260487117 1103 44-45 -0.15767377498093538 1103 46-47 -0.159433186513823 1103 48-49 -0.13320933973980686 1103 50-51 -0.11251887012162598 1103 52-53 -0.2140674960014337 1103 54-55 -0.16961470545228252 1103 56-57 -0.22039996856224775 1103 58-59 -0.18500554191518148 1103 60-61 -0.23619564878607235 1103 62-63 -0.1542041702189394 1103 64-65 -0.13290948996370844 1103 66-67 -0.18047433289233794 1103 68-69 -0.1503704786825253 1103 70-71 -0.2208439533496218 1103 72-73 -0.2166446711898189 1103 74-75 -0.2401763405563031 1103 76-77 -0.23732677166120908 1103 78-79 -0.26929896657188834 1103 80-81 -0.22408782193956256 1103 82-83 -0.22134166267500177 1103 84-85 -0.30030951563550445 1103 86-87 -0.24876601665607012 1103 88-89 -0.32246233956637127 1103 90-91 -0.4390550099613577 1103 92-93 -0.44439648196627246 1103 94-95 -0.40322636151154256 1103 96-97 -0.3812986895837227 1103 98-99 -0.3329925263065263 1103 100-101 -0.09376678330163202 2204 1 -0.27724990972583896 2204 2 -0.2560823282708711 2204 3 -0.14175036706934918 2204 4 -0.05755552790957097 2204 5 -0.05441130508877734 2204 6 -0.1468731700320518 2204 7 -0.09250508711356531 2204 8 -0.10201335412088497 2204 9 0.013514928333457021 2204 10-11 0.04523829006834035 2204 12-13 -0.003134493404274963 2204 14-15 -0.018367014004184057 2204 16-17 -7.994831524840151E-4 2204 18-19 0.004587935471903393 2204 20-21 -0.06635780587403417 2204 22-23 -0.0430063349684886 2204 24-25 -0.05026059614134226 2204 26-27 -0.09757497059160869 2204 28-29 -0.13879462347679805 2204 30-31 -0.06898799343565543 2204 32-33 -0.030105639950896546 2204 34-35 -0.025798340532318775 2204 36-37 -0.022476951293910474 2204 38-39 -0.07181522615938007 2204 40-41 -0.12298409949869438 2204 42-43 -0.14982472483194442 2204 44-45 -0.15769346747700297 2204 46-47 -0.16801928040904812 2204 48-49 -0.1595568902582798 2204 50-51 -0.11995666119017301 2204 52-53 -0.21803523637026956 2204 54-55 -0.1384020060415594 2204 56-57 -0.20024152372229764 2204 58-59 -0.11735675121143174 2204 60-61 -0.19293016363190674 2204 62-63 -0.1170811601547257 2204 64-65 -0.22227559488135284 2204 66-67 -0.21087262208694568 2204 68-69 -0.17196458670425585 2204 70-71 -0.20763427874769746 2204 72-73 -0.1880469230632258 2204 74-75 -0.1501918757361338 2204 76-77 -0.17857767461779162 2204 78-79 -0.2223834966810898 2204 80-81 -0.1982545172780874 2204 82-83 -0.27107353730829686 2204 84-85 -0.28612070218224517 2204 86-87 -0.2079986992975833 2204 88-89 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-0.10808590463173573 1215 18-19 -0.08376717947956536 1215 20-21 -0.029032862550330663 1215 22-23 -0.057287880812090464 1215 24-25 -0.09965360369369591 1215 26-27 -0.07348650776378918 1215 28-29 0.09359231808743118 1215 30-31 0.03296417174335886 1215 32-33 0.037234908498710695 1215 34-35 0.050706175678712384 1215 36-37 -0.08603742208719467 1215 38-39 0.0091867139605597 1215 40-41 9.592408112837347E-4 1215 42-43 0.07583796340077242 1215 44-45 0.17187513996805848 1215 46-47 0.19135691391556975 1215 48-49 0.09488843068027109 1215 50-51 0.15334206231568004 1215 52-53 0.15843048173606178 1215 54-55 0.16928220873374755 1215 56-57 0.14714676478960342 1215 58-59 0.2536163261531712 1215 60-61 0.2673003476385105 1215 62-63 0.19098330610616898 1215 64-65 0.2693473138786189 1215 66-67 0.2478377778663372 1215 68-69 0.20595020948008624 1215 70-71 0.24833305727036503 1215 72-73 0.2468624392393508 1215 74-75 0.22219188239386511 1215 76-77 0.279540232972721 1215 78-79 0.2578194501140487 1215 80-81 0.2535038577734028 1215 82-83 0.2812732363011463 1215 84-85 0.3215859784205364 1215 86-87 0.2708187235921784 1215 88-89 0.3418774498632544 1215 90-91 0.3078155555883555 1215 92-93 0.28473697586310465 1215 94-95 0.2936717338671926 1215 96-97 0.4224724731362173 1215 98-99 0.4003535770752933 1215 100-101 0.28139348109674955 1212 1 0.2796939659262918 1212 2 0.14990527940488363 1212 3 0.2034141193479968 1212 4 0.11950021765274954 1212 5 0.12350672848321409 1212 6 0.08592394459045494 1212 7 0.012062187665861757 1212 8 -0.08878986027684022 1212 9 -0.13134022617605723 1212 10-11 -0.04021883211935062 1212 12-13 -0.05946008806676417 1212 14-15 -0.022436868343383765 1212 16-17 0.003684476887407584 1212 18-19 -0.11783950554432465 1212 20-21 -0.01897871722641753 1212 22-23 -0.07203819403799372 1212 24-25 -0.007983092795775804 1212 26-27 0.01203088447677203 1212 28-29 0.054820076706000975 1212 30-31 0.05285870433685602 1212 32-33 0.07215831457935451 1212 34-35 0.008242088152684346 1212 36-37 0.02041361905284589 1212 38-39 0.026719317556064937 1212 40-41 0.08133958122046892 1212 42-43 0.1492001485540797 1212 44-45 0.17164164284811534 1212 46-47 0.12172450890945186 1212 48-49 0.2213966060068273 1212 50-51 0.2833973178397571 1212 52-53 0.19968134125073078 1212 54-55 0.15831587374810852 1212 56-57 0.09008640207050433 1212 58-59 0.16102030348410779 1212 60-61 0.20579629348071649 1212 62-63 0.20744635147974577 1212 64-65 0.22507426911694495 1212 66-67 0.22027378496019168 1212 68-69 0.18872467447260277 1212 70-71 0.2234098178349413 1212 72-73 0.09203889379656971 1212 74-75 0.2403370031559362 1212 76-77 0.2498942746675894 1212 78-79 0.18468371430815012 1212 80-81 0.06917864091785475 1212 82-83 0.14235672375205155 1212 84-85 0.2232982883783876 1212 86-87 0.1810097343000976 1212 88-89 0.31451358572189747 1212 90-91 0.30618284203444546 1212 92-93 0.23060834207263525 1212 94-95 0.2058456882670967 1212 96-97 0.29765710347526664 1212 98-99 0.2827789009564228 1212 100-101 -0.14414585214238684 1213 1 0.45351803195951135 1213 2 0.22451712321872463 1213 3 0.2741430664711473 1213 4 0.14762383869364015 1213 5 0.17618284219800984 1213 6 0.09993894026856509 1213 7 0.041574447852219976 1213 8 -0.05891787878933741 1213 9 -0.12199897473985288 1213 10-11 -0.2553727580836096 1213 12-13 -0.13235729688204856 1213 14-15 -0.09609100929245074 1213 16-17 -0.07069092566274549 1213 18-19 -0.03427035521244903 1213 20-21 -0.061931340948930824 1213 22-23 -0.06646107312911198 1213 24-25 -0.18497393149744568 1213 26-27 -0.11336331710648295 1213 28-29 -0.01890575330550348 1213 30-31 -0.17584124680234936 1213 32-33 -0.11737042049090718 1213 34-35 -0.1439471928942595 1213 36-37 -0.10191678839346707 1213 38-39 -0.2041547153989498 1213 40-41 -0.14048732668734942 1213 42-43 -0.11056211032098417 1213 44-45 -0.003233338810602504 1213 46-47 -0.15194644516986955 1213 48-49 -0.21637691458901287 1213 50-51 -0.31105086080112443 1213 52-53 -0.22533265091836086 1213 54-55 -0.2341831468168607 1213 56-57 -0.16596681403368052 1213 58-59 -0.10278670430793113 1213 60-61 -0.035778167991402654 1213 62-63 -0.08475733227188442 1213 64-65 -0.08304723435381334 1213 66-67 -0.16479726156865127 1213 68-69 -0.07702037734633649 1213 70-71 -0.11951626733401355 1213 72-73 -0.09818373719789975 1213 74-75 -0.07845122860106102 1213 76-77 -0.1312199014322104 1213 78-79 -0.0452375398555418 1213 80-81 -0.10284135656984006 1213 82-83 -0.10610725449043912 1213 84-85 -0.015123560708865114 1213 86-87 -0.14650825260553546 1213 88-89 -0.056268423378071475 1213 90-91 -0.04306704287438379 1213 92-93 -0.155562121112375 1213 94-95 -0.26798670447686845 1213 96-97 -0.2602237978981421 1213 98-99 -0.33200146452179524 1213 100-101 -1.1813684228846313 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 25.0 4 67.0 5 82.0 6 91.0 7 78.0 8 100.0 9 100.0 10 145.0 11 217.0 12 316.0 13 287.0 14 880.0 15 6098.0 16 22768.0 17 37306.0 18 39163.0 19 39212.0 20 43138.0 21 49894.0 22 58826.0 23 67109.0 24 75708.0 25 84515.0 26 94004.0 27 105357.0 28 120143.0 29 136895.0 30 156084.0 31 181883.0 32 215283.0 33 261223.0 34 330030.0 35 436775.0 36 639552.0 37 1108107.0 38 2849737.0 39 6024550.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 17.86608739321637 32.47677330426345 29.659225475894075 19.997913826626096 2 20.846920067219056 33.20584106662005 27.85207619859117 18.095162667569717 3 20.466881857422585 30.952359819335655 28.959072563193793 19.621685760047967 4 20.422232751128643 29.82611245504298 29.292610490609526 20.45904430321885 5 20.329799660785035 30.18887275084616 29.966787761408035 19.51453982696077 6 20.369629878632615 30.166980268850413 28.941717018981173 20.521672833535796 7 20.667381704226113 30.26065144164056 29.47224175500283 19.599725099130495 8 19.895070485247803 30.4790156899708 29.798398370790498 19.827515453990895 9 19.995742060809196 30.134134944362373 30.07176000331291 19.79836299151552 10-11 20.027631564226795 30.449077389807 29.6475686138652 19.875722432101007 12-13 19.896221132778788 30.355438126114144 29.881162612150064 19.867178128957004 14-15 19.961574525706297 30.309618708211712 29.856344685054538 19.872462081027457 16-17 20.07170880017308 30.09056508391566 29.90937953897877 19.928346576932494 18-19 19.97483334916917 30.008191014690155 29.90761923672791 20.109356399412764 20-21 20.133111291557032 30.01654542695693 29.72749821744234 20.1228450640437 22-23 20.192193160480247 29.904049466077424 29.698469358723894 20.205288014718434 24-25 20.28991967596021 29.87029364133219 29.613681460825564 20.226105221882033 26-27 20.393323217640134 29.85158866214413 29.54309934334734 20.211988776868395 28-29 20.340812226362935 29.897083236649834 29.493324599479955 20.268779937507276 30-31 20.377027056967165 29.72775347563768 29.59999778442988 20.29522168296527 32-33 20.402432673336737 29.8374902162872 29.44789055716556 20.3121865532105 34-35 20.47238413882661 29.801739125685234 29.402437834669726 20.323438900818434 36-37 20.417843343550956 29.796184295055085 29.4435501415616 20.342422219832358 38-39 20.4288853627932 29.719039304374878 29.481598807239646 20.37047652559228 40-41 20.507465991615163 29.798562912254674 29.390567361653176 20.303403734476994 42-43 20.502472344898383 29.7655159657425 29.400395145768183 20.331616543590933 44-45 20.492681537469192 29.692192697779767 29.421582573535098 20.39354319121594 46-47 20.52845552358539 29.754585086407655 29.33826688485372 20.378692505153236 48-49 20.468961839250298 29.68262099223117 29.402353152578765 20.44606401593977 50-51 20.48420631615322 29.74034457353087 29.364448383647524 20.411000726668387 52-53 20.497072061443845 29.75219305200097 29.37956805224754 20.371166834307644 54-55 20.501083871444436 29.699352028799257 29.39395407173696 20.405610028019343 56-57 20.526912848638354 29.756333676192305 29.319411349776274 20.397342125393067 58-59 20.495507839873405 29.803425204533486 29.32164427872349 20.37942267686962 60-61 20.46538547498918 29.73805689755863 29.3056437060355 20.49091392141669 62-63 20.43337448080149 29.717526120752645 29.365056787147424 20.484042611298438 64-65 20.489461632184366 29.738698062191148 29.322714999655012 20.449125305969467 66-67 20.474308027525918 29.71808793654186 29.294643467100336 20.512960568831886 68-69 20.407434090127317 29.745775609261667 29.319729057547733 20.527061243063287 70-71 20.54368469612162 29.734519565339934 29.25475292325198 20.467042815286465 72-73 20.496244630207528 29.756740098763252 29.282617763707623 20.464397507321593 74-75 20.51714899621192 29.726679207869566 29.29859819170895 20.457573604209557 76-77 20.488034834735224 29.78549898612639 29.255240091962172 20.471226087176213 78-79 20.484982401887876 29.767595371518418 29.30125592639711 20.44616630019659 80-81 20.526163295526214 29.771241629239263 29.235851311876598 20.46674376335792 82-83 20.496949874455687 29.84960857259587 29.26963898849863 20.38380256444981 84-85 20.501668530790752 29.773439308553495 29.259268083249218 20.465624077406535 86-87 20.49433887763323 29.77488519707286 29.24984798409535 20.480927941198562 88-89 20.522527310875354 29.770015977461973 29.233683137887787 20.473773573774885 90-91 20.5236353703507 29.735598737423558 29.235610118821796 20.50515577340395 92-93 20.478219841574223 29.75323333534281 29.280022890560804 20.488523932522167 94-95 20.524504816527912 29.766735838234894 29.222522853439862 20.48623649179733 96-97 20.562224022682 29.793787073225747 29.186088267593263 20.457900636498998 98-99 20.53744080303006 29.78790540989662 29.17615027190466 20.498503515168657 100-101 20.67605616489015 29.704927663514813 28.94095673568201 20.678059435913028 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 719.0 1 752.5 2 900.5 3 1111.5 4 1326.5 5 1519.0 6 1832.5 7 2313.0 8 2868.0 9 3436.5 10 3994.5 11 4736.0 12 5891.0 13 7104.5 14 8422.0 15 10374.0 16 12837.0 17 16761.5 18 22939.0 19 31510.5 20 43202.5 21 58499.5 22 77095.0 23 99011.0 24 124893.0 25 154449.5 26 186639.0 27 220644.0 28 256308.0 29 292182.5 30 326800.0 31 360284.0 32 392991.5 33 423011.5 34 451029.5 35 478110.0 36 502494.0 37 521833.5 38 532682.0 39 533115.5 40 523580.5 41 506147.0 42 481466.0 43 453311.5 44 425631.5 45 401443.5 46 382306.0 47 366767.0 48 350302.0 49 328828.5 50 305644.0 51 282782.0 52 258678.5 53 236453.0 54 217468.5 55 199639.0 56 182870.5 57 166639.5 58 150070.5 59 131751.5 60 112622.0 61 94633.0 62 78661.5 63 65141.0 64 54171.5 65 44869.5 66 36704.5 67 30133.0 68 24634.0 69 19808.0 70 15999.0 71 12935.0 72 10368.5 73 8602.0 74 7209.5 75 6018.0 76 5216.0 77 4636.5 78 4152.5 79 3673.0 80 3223.0 81 2766.0 82 2384.0 83 2104.0 84 1752.0 85 1384.5 86 1072.0 87 811.0 88 569.5 89 409.0 90 303.0 91 213.5 92 151.0 93 97.5 94 66.0 95 48.0 96 38.5 97 30.5 98 22.5 99 15.0 100 31.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02820469280888025 2 0.028614225858538636 3 0.030002087860158722 4 0.03870845713808142 5 0.022433310386842643 6 0.009988056044446168 7 0.010314165695100066 8 0.018375899617079014 9 0.007333675167030708 10-11 0.011766491232314524 12-13 0.01403788286884575 14-15 0.03526155397012335 16-17 0.025830917909934432 18-19 0.02746525813588595 20-21 0.013222608742211005 22-23 0.03791214287485679 24-25 0.04651233691768287 26-27 0.03473067779464026 28-29 0.0368162627697524 30-31 0.047756103957386116 32-33 0.04308439361313491 34-35 0.02823502859033643 36-37 0.03861365782103087 38-39 0.04729727526286144 40-41 0.02838291552493529 42-43 0.038249628443556755 44-45 0.0545702788669798 46-47 0.04494625220000775 48-49 0.05794513455397945 50-51 0.05392564351103604 52-53 0.03642568958350413 54-55 0.027336331064697194 56-57 0.03728646738232314 58-59 0.027427338409065728 60-61 0.03317976096769323 62-63 0.02952429930222394 64-65 0.03096145694871031 66-67 0.031924618009943916 68-69 0.03636501802059178 70-71 0.03766187267784333 72-73 0.030521588117595748 74-75 0.0313368622442305 76-77 0.03876912870099378 78-79 0.033092545596006724 80-81 0.03714616439308832 82-83 0.03901181495264319 84-85 0.04694462180343339 86-87 0.04707354887462214 88-89 0.047373114716501886 90-91 0.04815046911631641 92-93 0.04942836391015785 94-95 0.04885577603517252 96-97 0.053212752646815895 98-99 0.0520637849241632 100-101 0.05538176102093252 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1.3185749E7 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 94.58580544778312 #Duplication Level Percentage of deduplicated Percentage of total 1 98.11733537407325 92.8050719474698 2 1.2025567923387843 2.2748960560013276 3 0.22912799818998056 0.6501676877831251 4 0.13553510375212152 0.5127878781937306 5 0.0793617104563117 0.3753245652611996 6 0.04189806326356276 0.23777772362917532 7 0.03403281538912348 0.22533148786651663 8 0.01654875123850783 0.12522215720394092 9 0.011771572472521195 0.1002081297330332 >10 0.130494626426875 2.580676434925821 >50 0.0011744093925368728 0.06355827561332322 >100 1.5153405377492453E-4 0.02710199638598639 >500 0.0 0.0 >1k 1.1248952597520013E-5 0.021875659933060253 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 1.4409496191683917E-4 0.0 7.583945364044166E-6 2 1.4409496191683917E-4 0.0 3.0335781456176665E-5 3 1.4409496191683917E-4 7.583945364044166E-6 3.0335781456176665E-5 4 1.5167890728088332E-4 1.5167890728088332E-5 3.791972682022083E-5 5 1.6684679800897166E-4 2.27518360921325E-5 3.791972682022083E-5 6 1.8201468873706E-4 2.27518360921325E-5 4.5503672184265E-5 7 1.9718257946514832E-4 3.0335781456176665E-5 6.067156291235333E-5 8 1.9718257946514832E-4 3.0335781456176665E-5 7.583945364044166E-5 9 2.1993441555728083E-4 3.0335781456176665E-5 7.583945364044166E-5 10-11 2.654380877415458E-4 3.791972682022083E-5 7.583945364044166E-5 12-13 2.995658418797446E-4 3.791972682022083E-5 8.72153716865079E-5 14-15 3.602374047920979E-4 3.791972682022083E-5 1.1755115314268458E-4 16-17 4.3607685843253955E-4 3.791972682022083E-5 1.3651101655279502E-4 18-19 4.777885579347825E-4 3.791972682022083E-5 1.7822271605503792E-4 20-21 5.4604406621118E-4 3.791972682022083E-5 2.009745521471704E-4 22-23 5.95339711077467E-4 3.791972682022083E-5 2.085584975112146E-4 24-25 6.749711373999307E-4 3.791972682022083E-5 2.237263882393029E-4 26-27 7.356427003122842E-4 3.791972682022083E-5 2.4647822433143543E-4 28-29 7.887303178605933E-4 3.791972682022083E-5 2.881899238336783E-4 30-31 9.100734436853E-4 3.791972682022083E-5 3.0714978724378876E-4 32-33 0.001046584460238095 3.791972682022083E-5 3.2610965065389913E-4 34-35 0.0011717195587448237 3.791972682022083E-5 3.374855686999654E-4 36-37 0.0012930626845695302 4.1711699502242914E-5 3.6402937747412E-4 38-39 0.0014068218650301928 4.5503672184265E-5 3.981571316123187E-4 40-41 0.0015471248542650098 4.5503672184265E-5 4.171169950224292E-4 42-43 0.0016874278434998269 4.5503672184265E-5 4.436608037965837E-4 44-45 0.0018011870239604895 4.929564486628708E-5 4.474527764786058E-4 46-47 0.0019149462044211522 5.308761754830916E-5 4.5882869452467203E-4 48-49 0.0020704170843840576 6.067156291235333E-5 4.815805306168045E-4 50-51 0.002237263882393029 6.067156291235333E-5 5.232922301190475E-4 52-53 0.002449614352586266 6.82555082763975E-5 5.612119569392683E-4 54-55 0.0027188444130098336 6.82555082763975E-5 5.91547738395445E-4 56-57 0.0029918664461154234 6.82555082763975E-5 6.256754925336437E-4 58-59 0.0034051914684558307 7.204748095841957E-5 6.635952193538646E-4 60-61 0.0038185164907962375 7.583945364044166E-5 6.863470554459971E-4 62-63 0.004531407355016389 7.583945364044166E-5 7.128908642201517E-4 64-65 0.005316345700194961 8.72153716865079E-5 7.318507276302621E-4 66-67 0.006522193013077982 9.100734436853E-5 7.470186183583503E-4 68-69 0.008012438277112661 9.100734436853E-5 7.659784817684608E-4 70-71 0.010135942979045028 9.100734436853E-5 7.735624271325049E-4 72-73 0.013108849561750341 9.100734436853E-5 8.114821539527259E-4 74-75 0.017579585353854378 9.859128973257416E-5 8.494018807729466E-4 76-77 0.02377566871627846 1.0996720777864042E-4 8.759456895471012E-4 78-79 0.032110424671363 1.2134312582470666E-4 8.835296349111454E-4 80-81 0.043755572777852816 1.2134312582470666E-4 9.062814710032779E-4 82-83 0.059010678877627655 1.2134312582470666E-4 9.517851431875429E-4 84-85 0.07979068917510868 1.4409496191683917E-4 9.669530339156312E-4 86-87 0.10573157429282173 1.4409496191683917E-4 9.821209246437196E-4 88-89 0.13883928777955656 1.4409496191683917E-4 0.0010162486787819182 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGCCGTA 375 0.0 26.60679 46-47 ATTGGCG 1565 0.0 24.278597 1 TCGCCGT 565 0.0 18.079664 44-45 TTGGCGT 1580 0.0 16.833673 2 TCCGATC 910 0.0 11.746055 32-33 GTCGCCG 880 0.0 11.607966 44-45 GCCGTAT 900 0.0 11.35012 46-47 GCGTGAC 1745 0.0 10.885867 5 CCGTATC 1140 0.0 9.794278 48-49 ATTCGCG 490 2.2538105E-4 9.692858 1 CCGATCT 1090 0.0 9.370502 32-33 TTCCGAT 1125 0.0 9.290538 30-31 CGTATCA 1280 0.0 9.279818 48-49 GTGGTCG 1190 0.0 9.182284 40-41 TGGTCGC 1195 0.0 9.144108 42-43 AGATCGG 5125 0.0 8.944133 94-95 AGCGTCG 1745 0.0 8.846931 94-95 GCGGGAC 925 6.613482E-8 8.7278185 5 TTGGCGG 1285 4.7293724E-11 8.501051 2 GATCGGA 4535 0.0 8.484231 94-95 >>END_MODULE