FastQCFastQC Report
Fri 3 Apr 2015
H2K5YBCXX l01n02 10868-bar4.342000000043ff.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2K5YBCXX l01n02 10868-bar4.342000000043ff.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13185749
Sequences flagged as poor quality0
Sequence length101
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTA3750.026.6067946-47
ATTGGCG15650.024.2785971
TCGCCGT5650.018.07966444-45
TTGGCGT15800.016.8336732
TCCGATC9100.011.74605532-33
GTCGCCG8800.011.60796644-45
GCCGTAT9000.011.3501246-47
GCGTGAC17450.010.8858675
CCGTATC11400.09.79427848-49
ATTCGCG4902.2538105E-49.6928581
CCGATCT10900.09.37050232-33
TTCCGAT11250.09.29053830-31
CGTATCA12800.09.27981848-49
GTGGTCG11900.09.18228440-41
TGGTCGC11950.09.14410842-43
AGATCGG51250.08.94413394-95
AGCGTCG17450.08.84693194-95
GCGGGAC9256.613482E-88.72781855
TTGGCGG12854.7293724E-118.5010512
GATCGGA45350.08.48423194-95
Produced by FastQC (version 0.11.2)