FastQCFastQC Report
Fri 3 Apr 2015
H2K5YBCXX l01n02 10551-bar6.34200000004441.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2K5YBCXX l01n02 10551-bar6.34200000004441.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13509011
Sequences flagged as poor quality0
Sequence length101
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTA5300.034.06262646-47
TCGCCGT7450.023.59429244-45
GTCGCCG9750.021.19562144-45
TCCGATC9850.017.84381132-33
GCCGTAT11150.015.76511846-47
TGGTCGC13950.015.15458642-43
CCGATCT12050.014.98023432-33
GGTCGCC14750.014.33264242-43
CCGTATC13400.014.00454748-49
GTGGTCG14550.013.87645640-41
TTCCGAT13750.013.81990630-31
CGATCTA8450.012.64819634-35
GAGCGTC33100.012.3400059
CGTATCA15100.012.11324648-49
TCGGTGG18200.011.74614538-39
TCTCGGT17700.011.40596836-37
CTTCCGA17050.011.00577330-31
CGTCGTG19500.010.71674612-13
AGAGCGT38700.010.4307968
AGATCGG63850.09.67168494-95
Produced by FastQC (version 0.11.2)