##FastQC	0.11.2
>>Basic Statistics	pass
#Measure	Value
Filename	H2K5YBCXX l01n02 10551-bar6.34200000004441.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	13509011
Sequences flagged as poor quality	0
Sequence length	101
%GC	40
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.98521601618357	38.0	38.0	38.0	32.0	38.0
2	36.00708416034305	38.0	38.0	38.0	32.0	38.0
3	35.929616461190236	38.0	38.0	38.0	32.0	38.0
4	35.877296198811294	38.0	38.0	38.0	32.0	38.0
5	35.76914572058606	38.0	38.0	38.0	32.0	38.0
6	37.971991213864584	40.0	38.0	40.0	38.0	40.0
7	38.06073768094496	40.0	38.0	40.0	38.0	40.0
8	38.133357578878275	40.0	38.0	40.0	38.0	40.0
9	38.16803221198058	40.0	38.0	40.0	38.0	40.0
10-11	38.174809614116086	40.0	38.0	40.0	38.0	40.0
12-13	38.19809862468837	40.0	38.0	40.0	38.0	40.0
14-15	38.157422071830425	40.0	38.0	40.0	38.0	40.0
16-17	38.169746919297054	40.0	38.0	40.0	38.0	40.0
18-19	38.15763048827186	40.0	38.0	40.0	38.0	40.0
20-21	38.147363785550255	40.0	38.0	40.0	38.0	40.0
22-23	38.14811880011054	40.0	38.0	40.0	38.0	40.0
24-25	38.098238316631765	40.0	38.0	40.0	38.0	40.0
26-27	38.095581830527784	40.0	38.0	40.0	38.0	40.0
28-29	38.00757727564216	40.0	38.0	40.0	38.0	40.0
30-31	38.00066359410026	40.0	38.0	40.0	38.0	40.0
32-33	37.992007520017566	40.0	38.0	40.0	38.0	40.0
34-35	37.95656558426076	40.0	38.0	40.0	38.0	40.0
36-37	37.96611709769131	40.0	38.0	40.0	38.0	40.0
38-39	37.94694063836353	40.0	38.0	40.0	38.0	40.0
40-41	37.87600058212996	40.0	38.0	40.0	35.0	40.0
42-43	37.7382459752235	40.0	38.0	40.0	32.0	40.0
44-45	37.63461092747649	40.0	38.0	40.0	32.0	40.0
46-47	37.58709375541999	40.0	38.0	40.0	32.0	40.0
48-49	37.56309921577531	40.0	38.0	40.0	32.0	40.0
50-51	37.495709493463295	40.0	38.0	40.0	32.0	40.0
52-53	37.45973098992961	40.0	38.0	40.0	32.0	40.0
54-55	37.43914021537179	40.0	38.0	40.0	32.0	40.0
56-57	37.38314566477146	40.0	38.0	40.0	32.0	40.0
58-59	37.32847204728755	40.0	38.0	40.0	32.0	40.0
60-61	37.293172016811596	40.0	38.0	40.0	32.0	40.0
62-63	37.27294670201986	40.0	38.0	40.0	32.0	40.0
64-65	37.20473134561812	40.0	38.0	40.0	32.0	40.0
66-67	37.195107436066195	40.0	38.0	40.0	32.0	40.0
68-69	37.14668816244209	38.0	38.0	40.0	32.0	40.0
70-71	37.045053816300836	38.0	38.0	40.0	32.0	40.0
72-73	37.01023120789523	38.0	38.0	40.0	32.0	40.0
74-75	36.91143274663112	38.0	38.0	40.0	32.0	40.0
76-77	36.84864828372706	38.0	38.0	40.0	32.0	40.0
78-79	36.821793727164774	38.0	38.0	40.0	32.0	40.0
80-81	36.742892392344636	38.0	38.0	40.0	32.0	40.0
82-83	36.6642656150032	38.0	38.0	40.0	32.0	40.0
84-85	36.54061914673102	38.0	38.0	40.0	32.0	40.0
86-87	36.46649688122987	38.0	38.0	40.0	32.0	40.0
88-89	36.43043269414763	38.0	38.0	40.0	32.0	40.0
90-91	36.332456313789365	38.0	38.0	40.0	29.5	40.0
92-93	36.27829764888044	38.0	38.0	40.0	27.0	40.0
94-95	36.192653592479864	38.0	38.0	40.0	27.0	40.0
96-97	36.08556906941596	38.0	38.0	40.0	27.0	40.0
98-99	35.985780935406744	38.0	38.0	40.0	27.0	40.0
100-101	34.060569904044044	38.0	35.0	39.0	20.0	40.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
2201	1	0.138392610611362
2201	2	-0.021481880556436295
2201	3	-0.023275849274398297
2201	4	0.01636166644539827
2201	5	0.08445991521806206
2201	6	0.03093284981343203
2201	7	0.09481792657471289
2201	8	0.13790668286100072
2201	9	0.08165490722290514
2201	10-11	0.17873863147014646
2201	12-13	0.17873781891169216
2201	14-15	0.16602010162732483
2201	16-17	0.0866672586976236
2201	18-19	0.06826087620770238
2201	20-21	0.11006588711810394
2201	22-23	0.06829717336238161
2201	24-25	0.09833156638037366
2201	26-27	0.10847191685778768
2201	28-29	0.04691413852896176
2201	30-31	0.11386267051485532
2201	32-33	0.16641892523737312
2201	34-35	0.1435455757264279
2201	36-37	0.13389080435536016
2201	38-39	0.08317364039467634
2201	40-41	0.08445733892162366
2201	42-43	0.09627301063630966
2201	44-45	0.062293077631451865
2201	46-47	0.05529409438712207
2201	48-49	0.04139084471093213
2201	50-51	0.06303072858518988
2201	52-53	0.005400542547498333
2201	54-55	0.0047136193043826324
2201	56-57	-0.01127453549688795
2201	58-59	0.062284513810702435
2201	60-61	-0.07101605394286281
2201	62-63	0.04262512438172905
2201	64-65	0.018251966010261356
2201	66-67	-0.08463419100721836
2201	68-69	-0.04166575534441108
2201	70-71	-0.05105392239041606
2201	72-73	-0.01457804314660649
2201	74-75	-0.016438594902517423
2201	76-77	0.03217453832316863
2201	78-79	0.019196864768758815
2201	80-81	-0.05700014152117916
2201	82-83	-0.07452987673529776
2201	84-85	-0.027422847854609245
2201	86-87	0.0630015453421322
2201	88-89	-0.03175156597052364
2201	90-91	-0.07283530956058115
2201	92-93	-0.03760778117989361
2201	94-95	-0.06570031481645344
2201	96-97	-0.09447121103130485
2201	98-99	-0.10921080548892803
2201	100-101	0.030201716625008146
1101	1	0.06937726166640346
1101	2	0.08006604185790422
1101	3	0.13663652328096987
1101	4	0.03261816999697942
1101	5	0.04771141428577863
1101	6	0.10615420198402603
1101	7	0.17948104223569317
1101	8	0.14236783884877724
1101	9	0.13923144991700553
1101	10-11	0.1717448187518542
1101	12-13	0.16652851046396933
1101	14-15	0.14823151364308274
1101	16-17	0.09793005948458955
1101	18-19	0.12457928808485264
1101	20-21	0.14111752665225197
1101	22-23	0.058908464355631907
1101	24-25	0.11283606590995277
1101	26-27	0.10590362313797641
1101	28-29	0.17009349917162098
1101	30-31	0.07843326469078704
1101	32-33	0.10850116495205953
1101	34-35	0.06946738666585617
1101	36-37	0.09452915889007585
1101	38-39	0.057863064124788366
1101	40-41	0.06573639766921247
1101	42-43	0.016471634966485738
1101	44-45	0.047841277501269985
1101	46-47	-0.04364271604539738
1101	48-49	0.08408899641172951
1101	50-51	0.03086504897109421
1101	52-53	0.028643010231157007
1101	54-55	0.054324832668875445
1101	56-57	0.03491340251176922
1101	58-59	0.03239774038040366
1101	60-61	-0.004650496673647808
1101	62-63	0.04624265530711824
1101	64-65	0.014792300641651934
1101	66-67	-0.04403035678307532
1101	68-69	-0.001797331198488905
1101	70-71	-0.011846181455965166
1101	72-73	-0.0032109945235703208
1101	74-75	0.015769198927245043
1101	76-77	0.04986358639863653
1101	78-79	0.05573537010407392
1101	80-81	0.03957407916686151
1101	82-83	0.04699507907508149
1101	84-85	0.0351211887136742
1101	86-87	0.1482762997340359
1101	88-89	0.06838390479036605
1101	90-91	0.048059818634619944
1101	92-93	0.03781452418373732
1101	94-95	-0.04149324854205361
1101	96-97	-0.014101657717482396
1101	98-99	0.058514810141439
1101	100-101	0.27813869553888537
2202	1	-0.0013076767922513
2202	2	-0.054449605285221025
2202	3	-0.0035159112593419195
2202	4	-0.03569836438956031
2202	5	-0.041431668353489215
2202	6	-0.06708584101184556
2202	7	0.02935601170928237
2202	8	0.02712048111215637
2202	9	0.07122932744925947
2202	10-11	0.07777864746160645
2202	12-13	0.09727678453810285
2202	14-15	0.08967500268106932
2202	16-17	0.04196739446835096
2202	18-19	0.07025729316035978
2202	20-21	0.0873534760386363
2202	22-23	0.05302002859001931
2202	24-25	0.05030926524820245
2202	26-27	0.053444927214492566
2202	28-29	0.043082189447360975
2202	30-31	0.018972365584701834
2202	32-33	0.08896499091888899
2202	34-35	0.06625543772127429
2202	36-37	0.037385533922758896
2202	38-39	0.06790608755402161
2202	40-41	0.025297550252091128
2202	42-43	-0.004834767696394238
2202	44-45	0.008267350615611235
2202	46-47	-0.018262970158268388
2202	48-49	-0.04258749735559775
2202	50-51	-0.012665263533179427
2202	52-53	0.012343044090059152
2202	54-55	0.01218001420094339
2202	56-57	-0.005924146728020219
2202	58-59	-0.013064174492861014
2202	60-61	-0.06503324152527057
2202	62-63	-0.06388630848979915
2202	64-65	-0.061984506880286006
2202	66-67	-0.16536451020077436
2202	68-69	-0.11746201085982477
2202	70-71	-0.06076036571982968
2202	72-73	-0.09064829986213141
2202	74-75	-0.0514497282356956
2202	76-77	-0.009170908926272148
2202	78-79	-0.009804468475394401
2202	80-81	-0.003097844337041522
2202	82-83	-0.029830375508574036
2202	84-85	-0.021653569075738233
2202	86-87	-0.055425816866666366
2202	88-89	0.01669809523126986
2202	90-91	0.0067406898912096835
2202	92-93	0.002875083862612371
2202	94-95	-0.06120211562581801
2202	96-97	-0.02880066679624349
2202	98-99	9.33516618708552E-4
2202	100-101	0.1871212557911477
1102	1	-0.16043132454132092
1102	2	-0.03794964764833253
1102	3	-0.06994567408988672
1102	4	-0.15565616025907048
1102	5	-0.08504777983070966
1102	6	-0.21646864825735435
1102	7	-0.036489489143889386
1102	8	-0.039259706212298795
1102	9	-0.06501732785206826
1102	10-11	0.011783906304110303
1102	12-13	0.026325016251341538
1102	14-15	0.06640323836500528
1102	16-17	0.01044240381828132
1102	18-19	0.022510845990844075
1102	20-21	0.08038374540406323
1102	22-23	0.0028981643571057703
1102	24-25	-0.054167459177712374
1102	26-27	-0.04129476647984376
1102	28-29	0.013292622638800822
1102	30-31	0.037704384284964476
1102	32-33	0.04354055614654584
1102	34-35	-0.018849623769220614
1102	36-37	0.042734250204091495
1102	38-39	0.008241112891433033
1102	40-41	-0.014693732834516027
1102	42-43	-0.08901600310559843
1102	44-45	-0.16779412650181058
1102	46-47	-0.13806033875943058
1102	48-49	-0.13587631742947082
1102	50-51	-0.15578418522617454
1102	52-53	-0.1576614481448857
1102	54-55	-0.07001417792285025
1102	56-57	-0.21590189106769486
1102	58-59	-0.16089021972270956
1102	60-61	-0.2251232335741804
1102	62-63	-0.1910010082186986
1102	64-65	-0.23346446660720943
1102	66-67	-0.23530895732724133
1102	68-69	-0.2386906710473511
1102	70-71	-0.16346570128123972
1102	72-73	-0.17230511056144593
1102	74-75	-0.1930954799327793
1102	76-77	-0.16080855831440743
1102	78-79	-0.1953882622288745
1102	80-81	-0.1382946020950584
1102	82-83	-0.15787609432336325
1102	84-85	-0.1925722030097745
1102	86-87	-0.1164359609419705
1102	88-89	-0.1387066237287371
1102	90-91	-0.07926003638898038
1102	92-93	-0.2000369487932474
1102	94-95	-0.3105420168897126
1102	96-97	-0.23781150457963918
1102	98-99	-0.23920079286933316
1102	100-101	0.03748813780202198
2203	1	-0.16320909918479032
2203	2	-0.13988930461564308
2203	3	-0.2569171416915168
2203	4	-0.2629653282887574
2203	5	-0.20272170131112688
2203	6	-0.29527112820104406
2203	7	-0.20075232745510618
2203	8	-0.13274044840294152
2203	9	-0.11990940402822758
2203	10-11	-0.06404277875790143
2203	12-13	-0.0574761260590364
2203	14-15	-0.11000536420498008
2203	16-17	-0.05741151114884957
2203	18-19	-0.09707345980648086
2203	20-21	-0.08947950489498879
2203	22-23	-0.0827665974917764
2203	24-25	-0.06862483864470192
2203	26-27	-0.08116320558185208
2203	28-29	-0.04844432391105613
2203	30-31	-0.08587491023664029
2203	32-33	-0.06716179725525961
2203	34-35	-0.070272520129258
2203	36-37	-0.11154222449835771
2203	38-39	-0.1316351494445982
2203	40-41	-0.11446855963396985
2203	42-43	-0.1796492681092161
2203	44-45	-0.24654923182021804
2203	46-47	-0.21778659971725745
2203	48-49	-0.19397899297013765
2203	50-51	-0.21556450924454396
2203	52-53	-0.22677778377215674
2203	54-55	-0.21096980476512073
2203	56-57	-0.24927720443361778
2203	58-59	-0.22313967944403856
2203	60-61	-0.2602770431372221
2203	62-63	-0.2668717204645148
2203	64-65	-0.25508538159353833
2203	66-67	-0.2827915577508904
2203	68-69	-0.2557878976732866
2203	70-71	-0.20780098134254388
2203	72-73	-0.17335038811941672
2203	74-75	-0.12678461728098966
2203	76-77	-0.17901841162393595
2203	78-79	-0.2034860992927321
2203	80-81	-0.21585977256284394
2203	82-83	-0.20098729616822908
2203	84-85	-0.2838888618935087
2203	86-87	-0.21052713031074433
2203	88-89	-0.18415742388813783
2203	90-91	-0.2553202337031948
2203	92-93	-0.3240523375674087
2203	94-95	-0.2522081153510243
2203	96-97	-0.2676638695148057
2203	98-99	-0.2499324001156893
2203	100-101	-0.07054200357122653
1103	1	-0.07387711793290919
1103	2	-0.0025526189876572403
1103	3	-0.10903452576100392
1103	4	-0.06296225087913854
1103	5	-0.14485315705768897
1103	6	-0.15940894021041885
1103	7	-0.04978234618432964
1103	8	-0.05744811793535831
1103	9	-0.049554857685762954
1103	10-11	0.06448747308046876
1103	12-13	0.0809276740285938
1103	14-15	0.06305732630829652
1103	16-17	0.05985438828640355
1103	18-19	-0.005690059368404832
1103	20-21	0.042759937629263334
1103	22-23	0.014030681200033257
1103	24-25	0.022690518164203866
1103	26-27	0.024965766408641343
1103	28-29	0.01499975535638498
1103	30-31	-0.002360055082611723
1103	32-33	0.024834861560094623
1103	34-35	0.001070005074147673
1103	36-37	0.019669877488155407
1103	38-39	-0.07747495335115673
1103	40-41	-0.06330646493543668
1103	42-43	-0.050767072328596896
1103	44-45	-0.1256710734031472
1103	46-47	-0.11509452537549691
1103	48-49	-0.09408346566758752
1103	50-51	-0.0887392902292845
1103	52-53	-0.19368418281261057
1103	54-55	-0.14747953601150954
1103	56-57	-0.19864631072655214
1103	58-59	-0.16874590270420953
1103	60-61	-0.24501317644129017
1103	62-63	-0.08426106257050492
1103	64-65	-0.10759056204573625
1103	66-67	-0.15964240552828812
1103	68-69	-0.13683556539821495
1103	70-71	-0.17383601019975004
1103	72-73	-0.21172783492416158
1103	74-75	-0.191849015581397
1103	76-77	-0.21032386857304175
1103	78-79	-0.25335537791957563
1103	80-81	-0.22315321790605225
1103	82-83	-0.18747521861857308
1103	84-85	-0.31670204858755113
1103	86-87	-0.17136659045641522
1103	88-89	-0.21458809159626213
1103	90-91	-0.28424309694695893
1103	92-93	-0.33832395608915533
1103	94-95	-0.3410197531165764
1103	96-97	-0.3383120142361662
1103	98-99	-0.260920378255193
1103	100-101	0.03632783211210011
2204	1	-0.10142443378312294
2204	2	-0.08487957340839358
2204	3	-0.0182533448103257
2204	4	-0.08590897741461134
2204	5	-0.021765065789338678
2204	6	-0.11632216443061338
2204	7	-0.03004740304702125
2204	8	-0.09216062233792144
2204	9	0.009050157222098676
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1213	3	0.047426141876393046
1213	4	0.11909356976727992
1213	5	0.13638663369756188
1213	6	0.15539665766488042
1213	7	0.04802191038722725
1213	8	0.027349240223301763
1213	9	-0.048873039287528286
1213	10-11	-0.18187984674940338
1213	12-13	-0.07183373555009354
1213	14-15	-0.023398221773781813
1213	16-17	-0.053539635693759635
1213	18-19	-0.00940452322198837
1213	20-21	-0.05121953984385641
1213	22-23	-0.027925096896019852
1213	24-25	-0.11671490925493089
1213	26-27	-0.10796444357976753
1213	28-29	-0.03069898876388777
1213	30-31	-0.21882947865580604
1213	32-33	-0.0865431753720074
1213	34-35	-0.09647054200427618
1213	36-37	-0.10544464504269513
1213	38-39	-0.17195164493573145
1213	40-41	-0.10980531836892737
1213	42-43	-0.09374094603965233
1213	44-45	0.030576542639558113
1213	46-47	-0.1964211199504149
1213	48-49	-0.1897423286940736
1213	50-51	-0.31289144631969634
1213	52-53	-0.19579522836666996
1213	54-55	-0.19796562885871793
1213	56-57	-0.16183187825797773
1213	58-59	-0.0568681593249849
1213	60-61	-0.019170641676950595
1213	62-63	-0.09805108538160567
1213	64-65	-0.09690471394040401
1213	66-67	-0.14442044475443083
1213	68-69	-0.07246093194251557
1213	70-71	-0.09612610624672868
1213	72-73	-0.10983360062966341
1213	74-75	-0.1166060341113564
1213	76-77	-0.09726145847714207
1213	78-79	-0.07063175336469385
1213	80-81	-0.09922863871136656
1213	82-83	-0.1687851893928496
1213	84-85	0.0017425759007210218
1213	86-87	-0.1659980680393076
1213	88-89	-0.054912858571988465
1213	90-91	-0.03576912939907828
1213	92-93	-0.15099585401951288
1213	94-95	-0.2622707421006538
1213	96-97	-0.23567548419507034
1213	98-99	-0.3558390499128521
1213	100-101	-1.206279435870286
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	1.0
3	15.0
4	74.0
5	67.0
6	90.0
7	67.0
8	107.0
9	112.0
10	139.0
11	268.0
12	328.0
13	305.0
14	908.0
15	6540.0
16	28361.0
17	48563.0
18	49016.0
19	43758.0
20	43333.0
21	48087.0
22	55255.0
23	63092.0
24	70590.0
25	79058.0
26	88182.0
27	99286.0
28	112478.0
29	128437.0
30	148256.0
31	172832.0
32	204475.0
33	249528.0
34	315134.0
35	421956.0
36	618708.0
37	1074678.0
38	2794694.0
39	6542233.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	17.754394497047283	31.88116772327312	30.79539963388879	19.56903814579081
2	20.776009311394976	33.66744728112702	28.159846601002446	17.396696806475564
3	20.551186203980656	31.3750147629906	28.938586329341128	19.135212703687618
4	20.461453801338397	30.24775253286919	29.097813297440112	20.192980368352302
5	20.211035439269928	30.567656781315648	29.727125277414196	19.494182502000225
6	20.803140264263526	30.219653999107027	28.696051046025538	20.281154690603913
7	20.852575007177453	30.293248809889917	29.24810184271432	19.60607434021831
8	20.059562582839952	30.63469748489353	29.475815509401198	19.829924422865314
9	20.14666283782138	30.20148298906669	29.82303287336109	19.82882129975084
10-11	20.03427545361653	30.553353459683457	29.535439784046208	19.8769313026538
12-13	19.922999741432236	30.409523585655617	29.84301401540931	19.824462657502842
14-15	19.973635505213156	30.395482602325146	29.814683216003324	19.816198676458374
16-17	20.068012490207202	30.115537341359072	29.910216686058206	19.90623348237552
18-19	19.947197328907972	30.039355986751165	29.928224620049715	20.085222064291145
20-21	20.132128284994582	30.11740944221057	29.696647915152656	20.05381435764219
22-23	20.160170995982433	29.957128077332673	29.65465716198595	20.228043764698945
24-25	20.280436661058527	29.903310025019085	29.638992371336307	20.17726094258608
26-27	20.403772720884145	29.869679120712465	29.56565902504746	20.160889133355933
28-29	20.32872620594603	29.926846141337045	29.46518306718511	20.279244585531817
30-31	20.33816569126467	29.734859390474504	29.6415733247473	20.285401593513523
32-33	20.360429586993632	29.846419072822417	29.48207705351412	20.311074286669832
34-35	20.479935847134918	29.83183382305057	29.349273069328447	20.33895726048607
36-37	20.382987144959984	29.842117218814863	29.473114977046354	20.3017806591788
38-39	20.383454411285058	29.74079206694772	29.490943815544803	20.384809706222416
40-41	20.50054429201997	29.847494940544383	29.404471632080437	20.247489135355202
42-43	20.489126200880996	29.80422007494759	29.413042338488115	20.293611385683295
44-45	20.462937067494934	29.724626221412965	29.44872880025265	20.363707910839445
46-47	20.511747047778393	29.7948097302487	29.338618229797163	20.354824992175743
48-49	20.443439993532447	29.728253489003475	29.381155753799064	20.44715076366501
50-51	20.442063321627078	29.807269107675534	29.384446249888953	20.366221320808442
52-53	20.46129173266833	29.805838665487666	29.380315384707096	20.352554217136912
54-55	20.47326386479855	29.74826029397794	29.37304090786883	20.405434933354677
56-57	20.48262870775216	29.79462239495782	29.34822146083488	20.374527436455143
58-59	20.46730270168769	29.840875488029322	29.327682036196656	20.364139774086333
60-61	20.445963916371714	29.793857186826468	29.2931442972294	20.46703459957242
62-63	20.40680209582442	29.772495734649056	29.38731370987439	20.43338845965213
64-65	20.469143364429968	29.809470509610186	29.314612757703856	20.40677336825599
66-67	20.435376249200928	29.778856049949304	29.30559604547532	20.480171655374452
68-69	20.3953532484448	29.758949883407638	29.336641118747597	20.50905574939997
70-71	20.516048052517867	29.79935167237795	29.248095468186218	20.436504806917963
72-73	20.475104364684668	29.786805593736066	29.28862816923675	20.44946187234252
74-75	20.492149996447576	29.792997213263828	29.296835329693927	20.41801746059467
76-77	20.455416418990186	29.8179910172211	29.274382640373602	20.45220992341511
78-79	20.482350859534296	29.80129118135552	29.288688428939764	20.42766953017042
80-81	20.497158511855304	29.799560555796983	29.249083436008917	20.454197496338796
82-83	20.47479274211781	29.886190057649575	29.283064825539483	20.355952374693135
84-85	20.508014497391404	29.818920356253862	29.244445040203754	20.42862010615098
86-87	20.4569597750505	29.832851259194044	29.25550650222429	20.45468246353117
88-89	20.53220630386286	29.787500733646716	29.24604446389727	20.434248498593156
90-91	20.475562610462035	29.78760591570868	29.25267426020778	20.484157213621508
92-93	20.4507691273694	29.81118094808897	29.254249821930827	20.4838001026108
94-95	20.496791324285354	29.830623129111842	29.209743211886945	20.462842334715855
96-97	20.545512025258823	29.85095032945364	29.164872947666066	20.43866469762147
98-99	20.53560193607355	29.846069962220156	29.163305787890586	20.45502231381571
100-101	20.666027440461196	29.776826558886	28.91705595596894	20.640090044683866
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	735.0
1	731.0
2	805.5
3	937.0
4	1128.0
5	1369.0
6	1568.0
7	1801.5
8	2113.5
9	2469.0
10	2833.0
11	3329.5
12	3976.0
13	4828.0
14	6142.0
15	8067.0
16	10838.5
17	15314.0
18	21960.5
19	31440.0
20	44544.0
21	60824.0
22	80331.5
23	103925.5
24	131036.5
25	161238.0
26	194968.0
27	230893.0
28	267944.0
29	305413.5
30	342307.0
31	376944.5
32	410701.5
33	443051.5
34	472882.0
35	500636.5
36	525106.5
37	543830.0
38	551578.5
39	547550.5
40	533629.0
41	511683.0
42	484164.5
43	454225.0
44	425573.5
45	400726.0
46	380551.5
47	364769.5
48	348704.5
49	329752.0
50	309396.0
51	287174.0
52	263649.5
53	242034.5
54	222571.0
55	204947.0
56	188921.5
57	172277.0
58	154767.0
59	135686.5
60	116398.0
61	98086.5
62	81094.0
63	67257.0
64	55634.5
65	45690.5
66	37705.5
67	30822.5
68	25035.0
69	20296.5
70	16380.5
71	13174.0
72	10728.5
73	8832.0
74	7323.5
75	6193.5
76	5368.5
77	4741.0
78	4224.5
79	3713.0
80	3224.5
81	2789.0
82	2438.0
83	2102.0
84	1719.5
85	1351.0
86	1049.0
87	802.5
88	580.5
89	409.5
90	271.0
91	175.0
92	129.0
93	90.5
94	52.5
95	46.5
96	37.0
97	23.5
98	18.5
99	16.5
100	33.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.028314433972997727
2	0.02841066603617393
3	0.029284157071157915
4	0.03889255845598171
5	0.022451680585647612
6	0.010052549368713965
7	0.010637344214169342
8	0.01883927698334097
9	0.007565320658929065
10-11	0.012413936149729984
12-13	0.014164619452897032
14-15	0.03600929779389476
16-17	0.02628245694670024
18-19	0.02829962904020139
20-21	0.013583525840640738
22-23	0.037515699705922216
24-25	0.046772483936832976
26-27	0.03556885104320368
28-29	0.03706414925563389
30-31	0.047808829232576684
32-33	0.043689356681995445
34-35	0.027896194621501162
36-37	0.03822263524694739
38-39	0.04748682194425632
40-41	0.028518001798947385
42-43	0.03830776361052633
44-45	0.05503733767038905
46-47	0.04536971655437989
48-49	0.05752086514697486
50-51	0.05372339988471399
52-53	0.03650896427577119
54-55	0.02747795527000459
56-57	0.03706414925563389
58-59	0.027204064013272328
60-61	0.03305571370102519
62-63	0.02936928543473686
64-65	0.030498161560457683
66-67	0.03166775125136844
68-69	0.036349811248210545
70-71	0.0372788207811808
72-73	0.030227971536924504
74-75	0.030679521987212834
76-77	0.038285556211331825
78-79	0.03258195585154235
80-81	0.03632760384901604
82-83	0.03835958087531352
84-85	0.04565841274390849
86-87	0.04624320758936387
88-89	0.04632833595294282
90-91	0.04701676532797257
92-93	0.04836401421243939
94-95	0.04737578494828378
96-97	0.05208005234432039
98-99	0.05061806523068195
100-101	0.05381593071469111
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	1.3509011E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	95.61239004146887
#Duplication Level	Percentage of deduplicated	Percentage of total
1	98.17032178841053	93.86299097330017
2	1.137444980832553	2.175076663161463
3	0.2441218199876473	0.7002321201087655
4	0.1504410428175141	0.5753611065645391
5	0.09159994020519492	0.4379044605337162
6	0.019634373663353806	0.11263736357523171
7	0.03553939117406092	0.23786042915394542
8	0.026194676271109527	0.20036284837146667
9	0.02183536188359604	0.18789580233999065
>10	0.10259140036258625	1.456492761865585
>50	1.4440628732627603E-4	0.012840538149500702
>100	1.2162968014791261E-4	0.012908527905002668
>500	0.0	0.0
>1k	9.188424388274947E-6	0.02743640497061697
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA Adapter	Nextera Transposase Sequence
1	8.142713037986274E-5	0.0	7.402466398169341E-6
2	8.142713037986274E-5	0.0	2.2207399194508022E-5
3	8.882959677803209E-5	0.0	2.9609865592677363E-5
4	9.623206317620142E-5	7.402466398169341E-6	3.70123319908467E-5
5	1.1843946237070945E-4	1.4804932796338681E-5	4.4414798389016044E-5
6	1.258419287688788E-4	2.2207399194508022E-5	5.1817264787185385E-5
7	1.4064686156521747E-4	2.2207399194508022E-5	5.9219731185354725E-5
8	1.5545179436155616E-4	2.9609865592677363E-5	8.882959677803209E-5
9	1.6285426075972548E-4	2.9609865592677363E-5	1.110369959725401E-4
10-11	1.8876289315331818E-4	3.3311098791762036E-5	1.369456283661328E-4
12-13	2.220739919450802E-4	3.70123319908467E-5	1.6655549395881014E-4
14-15	2.7019002353318095E-4	3.70123319908467E-5	1.9246412635240284E-4
16-17	3.14604821922197E-4	3.70123319908467E-5	2.220739919450802E-4
18-19	3.70123319908467E-4	3.70123319908467E-5	2.4058015794050356E-4
20-21	4.0343441870022904E-4	3.70123319908467E-5	2.516838575377576E-4
22-23	4.5155045028832973E-4	3.70123319908467E-5	2.5538509073684226E-4
24-25	5.107701814736844E-4	3.70123319908467E-5	2.738912567322656E-4
26-27	6.033010114508012E-4	3.70123319908467E-5	2.997998891258583E-4
28-29	6.995330746270027E-4	3.70123319908467E-5	3.0350112232494295E-4
30-31	7.735577386086961E-4	3.70123319908467E-5	3.331109879176203E-4
32-33	8.660885685858129E-4	3.70123319908467E-5	3.59019620311213E-4
34-35	9.697230981601836E-4	4.071356518993137E-5	3.8122701950572103E-4
36-37	0.0010659551613363852	4.4414798389016044E-5	3.849282527048057E-4
38-39	0.0012103032561006873	4.4414798389016044E-5	4.145381182974831E-4
40-41	0.0013620538172631588	4.4414798389016044E-5	4.478492170892451E-4
42-43	0.0015249080780228841	4.4414798389016044E-5	4.6265414988558377E-4
44-45	0.0016396463071945088	4.4414798389016044E-5	4.922640154782611E-4
46-47	0.0017802931687597263	4.4414798389016044E-5	5.218738810709386E-4
48-49	0.0019764585283112137	4.4414798389016044E-5	5.625874462608699E-4
50-51	0.002146715255469109	4.4414798389016044E-5	6.07002244649886E-4
52-53	0.0023391793818215113	4.4414798389016044E-5	6.29209643844394E-4
54-55	0.002587162006160185	5.1817264787185385E-5	6.514170430389019E-4
56-57	0.002942480393272313	5.1817264787185385E-5	6.514170430389019E-4
58-59	0.003338512345574372	5.1817264787185385E-5	6.773256754324947E-4
60-61	0.0037937640290617866	5.1817264787185385E-5	7.254417070205953E-4
62-63	0.004519205736082383	5.1817264787185385E-5	7.550515726132727E-4
64-65	0.005496331300640735	5.1817264787185385E-5	7.772589718077808E-4
66-67	0.006832476485510301	5.9219731185354725E-5	7.994663710022888E-4
68-69	0.008361085796732271	5.9219731185354725E-5	8.179725369977121E-4
70-71	0.010792796008530899	6.662219758352406E-5	8.327774697940508E-4
72-73	0.014442211942828383	6.662219758352406E-5	8.401799361922201E-4
74-75	0.01952030389197255	7.40246639816934E-5	8.512836357894742E-4
76-77	0.02628245694670024	8.142713037986274E-5	8.845947345812362E-4
78-79	0.03528385608687416	8.142713037986274E-5	9.216070665720829E-4
80-81	0.04844544134281925	8.882959677803209E-5	9.549181653638449E-4
82-83	0.06566357818496113	9.623206317620142E-5	9.623206317620142E-4
84-85	0.08838544879414192	1.1843946237070945E-4	9.956317305537762E-4
86-87	0.11880218322421976	1.1843946237070945E-4	0.0010178391297482844
88-89	0.15850161051760192	1.1843946237070945E-4	0.0010289428293455383
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCCGTA	530	0.0	34.062626	46-47
TCGCCGT	745	0.0	23.594292	44-45
GTCGCCG	975	0.0	21.195621	44-45
TCCGATC	985	0.0	17.843811	32-33
GCCGTAT	1115	0.0	15.765118	46-47
TGGTCGC	1395	0.0	15.154586	42-43
CCGATCT	1205	0.0	14.980234	32-33
GGTCGCC	1475	0.0	14.332642	42-43
CCGTATC	1340	0.0	14.004547	48-49
GTGGTCG	1455	0.0	13.876456	40-41
TTCCGAT	1375	0.0	13.819906	30-31
CGATCTA	845	0.0	12.648196	34-35
GAGCGTC	3310	0.0	12.340005	9
CGTATCA	1510	0.0	12.113246	48-49
TCGGTGG	1820	0.0	11.746145	38-39
TCTCGGT	1770	0.0	11.405968	36-37
CTTCCGA	1705	0.0	11.005773	30-31
CGTCGTG	1950	0.0	10.716746	12-13
AGAGCGT	3870	0.0	10.430796	8
AGATCGG	6385	0.0	9.671684	94-95
>>END_MODULE