##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename H2K5YBCXX l01n01 64249-bar7.3410000000446d.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 11605012 Sequences flagged as poor quality 0 Sequence length 101 %GC 36 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 37.00949253650061 38.0 38.0 38.0 32.0 38.0 2 36.997197762484 38.0 38.0 38.0 32.0 38.0 3 36.97670411715214 38.0 38.0 38.0 32.0 38.0 4 36.96269439445646 38.0 38.0 38.0 32.0 38.0 5 36.95302400376665 38.0 38.0 38.0 32.0 38.0 6 38.874403145813204 40.0 38.0 40.0 38.0 40.0 7 38.82963567810184 40.0 38.0 40.0 38.0 40.0 8 38.81635383056907 40.0 38.0 40.0 38.0 40.0 9 38.83069220436825 40.0 38.0 40.0 38.0 40.0 10-11 38.82789401682652 40.0 38.0 40.0 38.0 40.0 12-13 38.81510755869964 40.0 38.0 40.0 38.0 40.0 14-15 38.80093247641622 40.0 38.0 40.0 38.0 40.0 16-17 38.789085698489586 40.0 38.0 40.0 38.0 40.0 18-19 38.74851555517564 40.0 38.0 40.0 38.0 40.0 20-21 38.72618989967438 40.0 38.0 40.0 38.0 40.0 22-23 38.70399229229578 40.0 38.0 40.0 38.0 40.0 24-25 38.66085088925371 40.0 38.0 40.0 38.0 40.0 26-27 38.58485109709494 40.0 38.0 40.0 38.0 40.0 28-29 38.524064817856285 40.0 38.0 40.0 38.0 40.0 30-31 38.49707341965696 40.0 38.0 40.0 38.0 40.0 32-33 38.44802870518359 40.0 38.0 40.0 38.0 40.0 34-35 38.41949702421678 40.0 38.0 40.0 38.0 40.0 36-37 38.38356879768845 40.0 38.0 40.0 38.0 40.0 38-39 38.34579111163349 40.0 38.0 40.0 38.0 40.0 40-41 38.29958439508722 40.0 38.0 40.0 38.0 40.0 42-43 38.237407897553226 40.0 38.0 40.0 38.0 40.0 44-45 38.21300555311791 40.0 38.0 40.0 38.0 40.0 46-47 38.171829206208486 40.0 38.0 40.0 38.0 40.0 48-49 38.1015391453279 40.0 38.0 40.0 38.0 40.0 50-51 38.07884304643545 40.0 38.0 40.0 38.0 40.0 52-53 38.04512571809491 40.0 38.0 40.0 38.0 40.0 54-55 37.97097685034707 40.0 38.0 40.0 38.0 40.0 56-57 37.95484976663531 40.0 38.0 40.0 38.0 40.0 58-59 37.91903653352534 40.0 38.0 40.0 38.0 40.0 60-61 37.853690414107284 40.0 38.0 40.0 32.0 40.0 62-63 37.82141164524431 40.0 38.0 40.0 32.0 40.0 64-65 37.75159508667462 40.0 38.0 40.0 32.0 40.0 66-67 37.6950664505991 40.0 38.0 40.0 32.0 40.0 68-69 37.64585344676938 40.0 38.0 40.0 32.0 40.0 70-71 37.61783020991275 40.0 38.0 40.0 32.0 40.0 72-73 37.58158944600832 40.0 38.0 40.0 32.0 40.0 74-75 37.48754430413342 40.0 38.0 40.0 32.0 40.0 76-77 37.420207665446625 40.0 38.0 40.0 32.0 40.0 78-79 37.33854510447728 40.0 38.0 40.0 32.0 40.0 80-81 37.294924770435394 40.0 38.0 40.0 32.0 40.0 82-83 37.21891037251836 40.0 38.0 40.0 32.0 40.0 84-85 37.17744548648463 40.0 38.0 40.0 32.0 40.0 86-87 37.12935260213432 38.0 38.0 40.0 32.0 40.0 88-89 37.0577568984849 38.0 38.0 40.0 32.0 40.0 90-91 36.99669491940206 38.0 38.0 40.0 32.0 40.0 92-93 36.911866355674604 38.0 38.0 40.0 32.0 40.0 94-95 36.834515380078884 38.0 38.0 40.0 32.0 40.0 96-97 36.74515558450091 38.0 38.0 40.0 32.0 40.0 98-99 36.71889985981919 38.0 38.0 40.0 32.0 40.0 100-101 35.882700121292416 38.0 38.0 40.0 29.5 40.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2201 1 0.1121322400972744 2201 2 0.10793114383499613 2201 3 0.03962619830086567 2201 4 0.0793734637489365 2201 5 -2.7063999588960996E-4 2201 6 0.04881662393798081 2201 7 0.07531847917981338 2201 8 0.010024456758451095 2201 9 0.031595420659222384 2201 10-11 0.04147476643830572 2201 12-13 0.023163777819888764 2201 14-15 -0.03375774881932614 2201 16-17 -0.19032143956889058 2201 18-19 -0.17541842053177703 2201 20-21 -0.0857138952009393 2201 22-23 -0.12299198022510893 2201 24-25 -0.13842207347619961 2201 26-27 -0.19598199869743382 2201 28-29 -0.19119706351715848 2201 30-31 -0.19359694582584552 2201 32-33 -0.21591156156109292 2201 34-35 -0.2375232452255105 2201 36-37 -0.2260472816720025 2201 38-39 -0.21297803507974322 2201 40-41 -0.23986467831660008 2201 42-43 -0.1921764148376539 2201 44-45 -0.30062438980240813 2201 46-47 -0.2458660150315879 2201 48-49 -0.2589487545889213 2201 50-51 -0.35292220835940924 2201 52-53 -0.2477093278986544 2201 54-55 -0.22123512002244183 2201 56-57 -0.2585064854392556 2201 58-59 -0.2998585169534991 2201 60-61 -0.26504051763861725 2201 62-63 -0.23710671740959555 2201 64-65 -0.3357655058333151 2201 66-67 -0.28843052868667485 2201 68-69 -0.30365270978508363 2201 70-71 -0.35261346505294 2201 72-73 -0.35613075440485886 2201 74-75 -0.30610835775822665 2201 76-77 -0.3230284397217531 2201 78-79 -0.28663028154488046 2201 80-81 -0.32105314746579694 2201 82-83 -0.37608343924328835 2201 84-85 -0.38310681689238635 2201 86-87 -0.45093671442320016 2201 88-89 -0.4427344449609478 2201 90-91 -0.3918310028669225 2201 92-93 -0.48117383847807105 2201 94-95 -0.4145918533882238 2201 96-97 -0.48247423232856335 2201 98-99 -0.44449608217436776 2201 100-101 -0.4929437351307513 1101 1 0.0868994575597668 1101 2 0.11930700624439083 1101 3 0.06126297795857738 1101 4 0.05025951993306421 1101 5 0.08108558503344199 1101 6 0.08413885762951878 1101 7 0.09419103231457626 1101 8 0.09174043738165238 1101 9 0.08761161813981033 1101 10-11 0.05760252307582192 1101 12-13 0.07370587511759652 1101 14-15 0.025165061300512548 1101 16-17 -0.01345872594354347 1101 18-19 -0.038867062469940095 1101 20-21 -0.0614049392393099 1101 22-23 -0.08378675096675181 1101 24-25 -0.052311099948383344 1101 26-27 -0.023249598967957752 1101 28-29 -0.09049422882373648 1101 30-31 -0.12990857036236036 1101 32-33 -0.13241496536245023 1101 34-35 -0.0976227576825579 1101 36-37 -0.13456981815125602 1101 38-39 -0.11367656738034526 1101 40-41 -0.06832182094348127 1101 42-43 -0.08061162491531348 1101 44-45 -0.09468236137656305 1101 46-47 -0.16345689164181465 1101 48-49 -0.13962517217068893 1101 50-51 -0.26030130734348944 1101 52-53 -0.16674528381394538 1101 54-55 -0.22215402298195386 1101 56-57 -0.16817019278722256 1101 58-59 -0.25483381240406544 1101 60-61 -0.2656137894450765 1101 62-63 -0.2940764216803231 1101 64-65 -0.33213575120654326 1101 66-67 -0.32987114693506925 1101 68-69 -0.3421437880075402 1101 70-71 -0.30483227828761983 1101 72-73 -0.32432901135231873 1101 74-75 -0.3099975067961438 1101 76-77 -0.28460225417537544 1101 78-79 -0.2561337291676793 1101 80-81 -0.31963999401418164 1101 82-83 -0.35361887920723944 1101 84-85 -0.3272894990938937 1101 86-87 -0.3234804133023701 1101 88-89 -0.4210721951377394 1101 90-91 -0.42177533368155196 1101 92-93 -0.39262351315841215 1101 94-95 -0.327365485571498 1101 96-97 -0.3753551741639072 1101 98-99 -0.3583729654051595 1101 100-101 -0.4469425427173377 2202 1 0.04303464367694687 2202 2 0.06866166340847712 2202 3 0.03737893081870425 2202 4 0.07747535306097575 2202 5 0.05556784708271323 2202 6 -0.03774559588995885 2202 7 0.025650812074758278 2202 8 -0.01667500883262818 2202 9 -0.017576522289850516 2202 10-11 0.044031848148016195 2202 12-13 -0.009522823395712976 2202 14-15 -0.01489637482016093 2202 16-17 -0.05123551308314234 2202 18-19 -0.0020652974506703003 2202 20-21 -0.05276422515274248 2202 22-23 -0.09918511992145795 2202 24-25 -0.02901016115175281 2202 26-27 -0.09227555250458863 2202 28-29 -0.09565453826502335 2202 30-31 -0.1021550547247898 2202 32-33 -0.13704835518193903 2202 34-35 -0.13300825755678147 2202 36-37 -0.09835294218677149 2202 38-39 -0.14694844716588307 2202 40-41 -0.20429078687564584 2202 42-43 -0.17593125261372933 2202 44-45 -0.18994784443741963 2202 46-47 -0.15073582052927748 2202 48-49 -0.17121175588895454 2202 50-51 -0.22752424720076192 2202 52-53 -0.24233024853914742 2202 54-55 -0.23416365266116657 2202 56-57 -0.2669809919497297 2202 58-59 -0.26169271821617457 2202 60-61 -0.18131665614301795 2202 62-63 -0.233392926804747 2202 64-65 -0.20990508490870496 2202 66-67 -0.2146431366490802 2202 68-69 -0.26493996976889633 2202 70-71 -0.3157150556189947 2202 72-73 -0.2617116716009349 2202 74-75 -0.2758971932218728 2202 76-77 -0.3135974544698499 2202 78-79 -0.29908584976517005 2202 80-81 -0.34743238262409193 2202 82-83 -0.3324929655656206 2202 84-85 -0.41307513821740827 2202 86-87 -0.3300852355557211 2202 88-89 -0.36288110875078416 2202 90-91 -0.41179094301372743 2202 92-93 -0.45741545548977314 2202 94-95 -0.3698276766037907 2202 96-97 -0.42001789029777115 2202 98-99 -0.4106038196014765 2202 100-101 -0.44619000143951837 1102 1 0.06684878870015609 1102 2 0.08269443669504284 1102 3 0.05250818857128792 1102 4 0.06443831893785301 1102 5 0.038681886629802875 1102 6 -0.03751919763420375 1102 7 -0.10886103573223238 1102 8 -0.061596019489748244 1102 9 -0.09616002302001192 1102 10-11 -0.0551871522463685 1102 12-13 -0.04825091214364363 1102 14-15 -0.035215824665058904 1102 16-17 -0.01864277369563183 1102 18-19 -0.034620745744341264 1102 20-21 -0.05900170082646383 1102 22-23 -0.18604976011785368 1102 24-25 -0.10615138260399704 1102 26-27 -0.1343141826462002 1102 28-29 -0.18966224715815372 1102 30-31 -0.1569776021710112 1102 32-33 -0.1359043837302849 1102 34-35 -0.17231851841125945 1102 36-37 -0.13948687715254948 1102 38-39 -0.14180793531728142 1102 40-41 -0.17238138528755798 1102 42-43 -0.19131878409949366 1102 44-45 -0.20392354736093665 1102 46-47 -0.2548809261026932 1102 48-49 -0.22199877126095657 1102 50-51 -0.22002762834680567 1102 52-53 -0.22459162049188564 1102 54-55 -0.26667829836543433 1102 56-57 -0.24368131146349015 1102 58-59 -0.2664885138108346 1102 60-61 -0.22542089026671164 1102 62-63 -0.29198611287122844 1102 64-65 -0.3054850878202444 1102 66-67 -0.31061976545849035 1102 68-69 -0.33076650763227633 1102 70-71 -0.3258396503679677 1102 72-73 -0.29208250170231054 1102 74-75 -0.3530421354137374 1102 76-77 -0.3410071407915254 1102 78-79 -0.4311225059745212 1102 80-81 -0.49669055825985 1102 82-83 -0.496265014110314 1102 84-85 -0.46614209766341475 1102 86-87 -0.4672691983385846 1102 88-89 -0.4480069271018863 1102 90-91 -0.48529967447301203 1102 92-93 -0.6433886894096119 1102 94-95 -0.5458594252412752 1102 96-97 -0.6034473652475754 1102 98-99 -0.6062003337706443 1102 100-101 -0.5890875593769636 2203 1 0.0861394447753625 2203 2 0.060524711466229064 2203 3 0.03937932482766371 2203 4 0.0864453946881909 2203 5 -0.005230392216112989 2203 6 -0.08356126607886694 2203 7 -0.11468854631076653 2203 8 -0.08227209202315322 2203 9 -0.10092350572199393 2203 10-11 -0.051733267145557704 2203 12-13 -0.08382367929398526 2203 14-15 -0.05384061945105145 2203 16-17 -0.07748740166539392 2203 18-19 -0.06278636747695288 2203 20-21 -0.08743737824227082 2203 22-23 -0.1258239027427095 2203 24-25 -0.059094525654835195 2203 26-27 -0.07241090338148837 2203 28-29 -0.10635501219837096 2203 30-31 -0.17615287450698247 2203 32-33 -0.1350006535734849 2203 34-35 -0.1655800656157993 2203 36-37 -0.19361663465671342 2203 38-39 -0.18525213117211337 2203 40-41 -0.2004360870112336 2203 42-43 -0.1746106819033102 2203 44-45 -0.19770862372715214 2203 46-47 -0.15172837046492305 2203 48-49 -0.1474036680284314 2203 50-51 -0.18298119383899092 2203 52-53 -0.20633853270183522 2203 54-55 -0.21077058359152545 2203 56-57 -0.2386839532724636 2203 58-59 -0.2910627878390599 2203 60-61 -0.3051329962629694 2203 62-63 -0.3433637500804423 2203 64-65 -0.37287895825161144 2203 66-67 -0.3222321875034524 2203 68-69 -0.3972719112821608 2203 70-71 -0.3776158046226783 2203 72-73 -0.3437123581186796 2203 74-75 -0.34924349170527336 2203 76-77 -0.46801829799608896 2203 78-79 -0.3874166562098438 2203 80-81 -0.4222484761288783 2203 82-83 -0.48459337385820334 2203 84-85 -0.5494139271260465 2203 86-87 -0.5031378167375138 2203 88-89 -0.5086981929189562 2203 90-91 -0.5169595116630603 2203 92-93 -0.7055255582632753 2203 94-95 -0.6594923492732931 2203 96-97 -0.6305855174237109 2203 98-99 -0.6160174231989828 2203 100-101 -0.6504639340145246 1103 1 0.04317123360858233 1103 2 0.0418915858950939 1103 3 0.07497768610468114 1103 4 0.03287327839414189 1103 5 0.08154136555170766 1103 6 -0.08385124854915915 1103 7 -0.08163036402088863 1103 8 -0.07408590608814336 1103 9 -0.068617869994128 1103 10-11 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28-29 -0.05422601377611613 2204 30-31 -0.01469082820558043 2204 32-33 0.0409488831570215 2204 34-35 0.019857879872724027 2204 36-37 -0.008810741010485401 2204 38-39 0.031212553811002408 2204 40-41 0.08035825797234963 2204 42-43 0.07293010780046671 2204 44-45 0.08020285733596921 2204 46-47 0.030967185965522503 2204 48-49 0.024067519300686513 2204 50-51 0.035975276304938575 2204 52-53 0.04354706658642726 2204 54-55 0.023167913985247424 2204 56-57 0.06879921028210845 2204 58-59 0.03207926250158977 2204 60-61 0.08168690927362121 2204 62-63 0.04876332870709632 2204 64-65 0.027856335373385832 2204 66-67 0.09241702708266075 2204 68-69 0.03327685737799868 2204 70-71 0.009571762328960176 2204 72-73 0.052362183011908314 2204 74-75 0.036563882966447636 2204 76-77 0.008722829504300478 2204 78-79 0.04021554239672298 2204 80-81 0.04851523114789558 2204 82-83 0.022019511769727274 2204 84-85 -0.044758462474554506 2204 86-87 -0.08122105992583073 2204 88-89 0.008793470868859288 2204 90-91 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1214 88-89 0.47143932155827883 1214 90-91 0.5952512135105934 1214 92-93 0.5393055823397788 1214 94-95 0.5345607074039691 1214 96-97 0.5453149540017179 1214 98-99 0.5941557182184738 1214 100-101 0.6687388143614683 1215 1 -0.11356383170073059 1215 2 -0.07203455108202661 1215 3 -0.03766317317380441 1215 4 0.040776823344018 1215 5 -0.05673344308484474 1215 6 0.05493475625294053 1215 7 0.024641492145875077 1215 8 0.021874940464677195 1215 9 0.061368029940396696 1215 10-11 0.0034972456034765287 1215 12-13 0.01785811094286771 1215 14-15 0.06233003719191288 1215 16-17 0.03117380143847015 1215 18-19 0.07005401439396053 1215 20-21 0.007358602180481455 1215 22-23 0.057845477243219534 1215 24-25 0.02482607237082135 1215 26-27 0.040081452369186366 1215 28-29 0.08559956355087195 1215 30-31 0.0975411007426743 1215 32-33 0.053192194562484474 1215 34-35 0.05962179019856961 1215 36-37 0.120618539782825 1215 38-39 0.08493386012483484 1215 40-41 0.07715428609016328 1215 42-43 0.10748150694961822 1215 44-45 0.14543914909251754 1215 46-47 0.14259336925950805 1215 48-49 0.1447684138917822 1215 50-51 0.21703210628709968 1215 52-53 0.23856857873010995 1215 54-55 0.2360474480119592 1215 56-57 0.24670662992809866 1215 58-59 0.17635903699584077 1215 60-61 0.25639480495146927 1215 62-63 0.23696830259573431 1215 64-65 0.3177948175699967 1215 66-67 0.3865748369718105 1215 68-69 0.36397731838502523 1215 70-71 0.32145869565333385 1215 72-73 0.3819461181270043 1215 74-75 0.3303516852451125 1215 76-77 0.3744646088310333 1215 78-79 0.35519751643701625 1215 80-81 0.4285874932904292 1215 82-83 0.4341125187032162 1215 84-85 0.4093442871418631 1215 86-87 0.46071840011141063 1215 88-89 0.4979129667065578 1215 90-91 0.5062218844070898 1215 92-93 0.5519699120505237 1215 94-95 0.5762878478654443 1215 96-97 0.587604476465323 1215 98-99 0.5622969390540575 1215 100-101 0.6281034481831682 1212 1 -0.10055229819312217 1212 2 -0.07764004116074119 1212 3 -0.09349296665038054 1212 4 -0.015789553738017048 1212 5 -0.04011271680541029 1212 6 -0.053905185888815765 1212 7 -0.08046978522456527 1212 8 -0.19555327804702927 1212 9 -0.16596035668882791 1212 10-11 -0.1862022083813173 1212 12-13 -0.21192591818577 1212 14-15 -0.1050667230983251 1212 16-17 0.005886948346187637 1212 18-19 -0.0067792734625555795 1212 20-21 0.006293517520248315 1212 22-23 0.03872009325751691 1212 24-25 0.03590852037321923 1212 26-27 0.04538940670459368 1212 28-29 0.13000703583202267 1212 30-31 0.05428632200938921 1212 32-33 0.032179246872566125 1212 34-35 0.03996841156445896 1212 36-37 0.028786451927480528 1212 38-39 0.011141639301143869 1212 40-41 -0.04720358418647663 1212 42-43 -0.035478194722458056 1212 44-45 0.06978944381163643 1212 46-47 0.05828817580699308 1212 48-49 0.12247590700424382 1212 50-51 0.1251659489607917 1212 52-53 0.07174607621421814 1212 54-55 0.005179456943025684 1212 56-57 0.03383270633501212 1212 58-59 0.17361579323716825 1212 60-61 -0.15370830712981132 1212 62-63 -0.07932323020771292 1212 64-65 0.005410834798375674 1212 66-67 0.0210208207820628 1212 68-69 0.19345809636133993 1212 70-71 0.10779124561181419 1212 72-73 0.24944282179126276 1212 74-75 0.23595012788417336 1212 76-77 0.2198494219213174 1212 78-79 0.3101920210771212 1212 80-81 0.3416000712978402 1212 82-83 0.2880020305755977 1212 84-85 0.3379548500861773 1212 86-87 0.33442809958300046 1212 88-89 0.3648045939844309 1212 90-91 0.3245202592710257 1212 92-93 0.4266249835958291 1212 94-95 0.35094263187990293 1212 96-97 0.39530644781198987 1212 98-99 0.4042524363210447 1212 100-101 0.4594149761162072 1213 1 -0.23113561189381215 1213 2 -0.14579134319152587 1213 3 -0.2022858898625799 1213 4 -0.16726306987468575 1213 5 -0.128989591965194 1213 6 -0.049090201093505925 1213 7 -0.01593496741124767 1213 8 -0.036455396669346385 1213 9 -0.021789314678997584 1213 10-11 -0.06929341770460695 1213 12-13 -0.12256942397104353 1213 14-15 -0.1444382339349275 1213 16-17 -0.24637943182961664 1213 18-19 -0.28161308552152065 1213 20-21 -0.13407773972674875 1213 22-23 -0.20468366379186165 1213 24-25 -0.22006588423342777 1213 26-27 -0.07085820589853853 1213 28-29 -0.10555079039818338 1213 30-31 -0.054321876360475585 1213 32-33 -0.09992684421757048 1213 34-35 -0.10104802631155962 1213 36-37 -0.006445524290377591 1213 38-39 0.03163658638106881 1213 40-41 0.08023471672564852 1213 42-43 0.11352734495110184 1213 44-45 0.12642444382490226 1213 46-47 0.10505496666588954 1213 48-49 0.12744388395290684 1213 50-51 0.13014489299607845 1213 52-53 0.2172360592955087 1213 54-55 0.190057025994399 1213 56-57 0.21469885304264835 1213 58-59 0.2504865118085817 1213 60-61 0.2551340359500429 1213 62-63 0.26407405600158285 1213 64-65 0.2545241516707222 1213 66-67 0.27366786698113543 1213 68-69 0.3072103212588644 1213 70-71 0.29453057149335393 1213 72-73 0.312251605327063 1213 74-75 0.33999701198531085 1213 76-77 0.3499961918391534 1213 78-79 0.3824979034818554 1213 80-81 0.4055263193882368 1213 82-83 0.40490543158311 1213 84-85 0.43904295470104415 1213 86-87 0.3936066382504251 1213 88-89 0.4568983959813835 1213 90-91 0.465758366310439 1213 92-93 0.540755343152469 1213 94-95 0.45424548914262175 1213 96-97 0.4799156404706224 1213 98-99 0.5386796264867684 1213 100-101 0.31210410705357106 >>END_MODULE >>Per sequence quality scores pass #Quality Count 13 26.0 14 52.0 15 110.0 16 218.0 17 413.0 18 1002.0 19 2182.0 20 4523.0 21 7945.0 22 13776.0 23 21754.0 24 31873.0 25 44470.0 26 58748.0 27 75876.0 28 94996.0 29 116249.0 30 140175.0 31 168108.0 32 201331.0 33 240680.0 34 294140.0 35 377322.0 36 520534.0 37 824051.0 38 1805255.0 39 6559203.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 16.12261123494769 31.32889982380202 33.46936336135833 19.079125579891958 2 17.953464356776657 33.37482982546226 32.16921124581075 16.50249457195034 3 17.96712489396823 32.42925556647421 32.09734724961939 17.506272289938174 4 18.11831818872742 31.812237677996368 31.969928165520205 18.099515967756 5 18.617301610554343 31.67713081768594 32.14746611286806 17.558101458891656 6 18.484849477105236 32.29927724331522 31.023931728808208 18.19194155077134 7 18.578558988133747 32.168669881599435 31.615684671416112 17.63708645885071 8 18.09889776763349 32.59825899091374 31.352200612943015 17.950642628509762 9 18.003490164383795 31.86758263590223 32.09949045254511 18.029436747168862 10-11 18.042820636462935 32.25757543378671 31.75657638268707 17.943027547063288 12-13 17.790643973634985 32.282421505934316 31.98419233293507 17.942742187495625 14-15 17.7618667917509 32.20390867558189 32.12015152633402 17.914073006333187 16-17 17.857830070942008 32.021267694462765 32.18818955657346 17.932712678021765 18-19 17.80130429852205 31.925490469118 32.22400804066381 18.04919719169614 20-21 17.908331843673768 31.87563843706857 32.162342431609545 18.053687287648113 22-23 17.91175374837629 31.897744173272336 32.12917476070534 18.061327317646032 24-25 17.93372386876958 31.775472786208887 32.13208608439073 18.158717260630812 26-27 18.008493508666845 31.766622055150933 32.02989204220007 18.194992393982158 28-29 18.063741506818996 31.820468288856933 32.00194884463785 18.113841359686223 30-31 18.05093751824421 31.73433281278309 32.06600855730314 18.148721111669552 32-33 18.07554007979888 31.814258634064167 31.97407355001959 18.13612773611736 34-35 18.069751750192168 31.809660785009875 32.0265519059946 18.094035558803352 36-37 18.106435213754672 31.75352570922009 31.979029082506848 18.16100999451839 38-39 18.15622758999303 31.70486187518213 31.978852386168743 18.1600581486561 40-41 18.13898943404392 31.77295554582026 31.974535636935908 18.11351938319991 42-43 18.12376344971974 31.70494233999871 32.00230908904748 18.16898512123407 44-45 18.151275451604352 31.65266015918094 31.992620293494838 18.203444095719874 46-47 18.1510074195169 31.729934635014 32.01383889449142 18.105219050977674 48-49 18.16374923841607 31.622171291240853 32.0302238560125 18.18385561433058 50-51 18.09697293681004 31.657192555835607 32.00745335116624 18.238381156188108 52-53 18.171150556492496 31.654755529708005 32.066292935326246 18.10780097847325 54-55 18.1280698472705 31.612040637606526 32.073931904185535 18.185957610937432 56-57 18.143673745231226 31.62116288095525 32.04507787423565 18.190085499577872 58-59 18.157307975911728 31.62498180056888 32.08285557358036 18.134854649939033 60-61 18.120698201241456 31.60959056389412 32.02202293465402 18.247688300210402 62-63 18.130218046899056 31.597153315133387 32.10077263738435 18.171856000583205 64-65 18.10656221004768 31.694669045851004 32.04696670154442 18.151802042556895 66-67 18.108733921613553 31.67728110821853 32.009406980067794 18.20457799010012 68-69 18.074515619774097 31.668685967905997 32.01937994884283 18.237418463477077 70-71 18.16601661313969 31.66787439519859 32.00964045710478 18.156468534556936 72-73 18.145544924649133 31.67933853133558 32.00953257187538 18.165583972139906 74-75 18.129126258778268 31.66827551256106 32.03620420391432 18.16639402474635 76-77 18.13502147930483 31.67454964531014 32.026560870500475 18.163868004884556 78-79 18.149796060917474 31.66078991035456 32.02018801453764 18.169226014190333 80-81 18.165297622472192 31.650767567604838 32.0236233655668 18.160311444356168 82-83 18.134477997388487 31.711715385278193 32.055057704259994 18.098748913073322 84-85 18.142451891726413 31.697878128522355 32.0047339054884 18.15493607426283 86-87 18.10389420560174 31.70611669327178 32.0216117611997 18.16837733992678 88-89 18.18204892147739 31.66070728937218 32.00619977485137 18.15104401429906 90-91 18.141461332145727 31.65348565137316 31.993794377884914 18.211258638596192 92-93 18.13185392552968 31.68236973916332 31.995829329675406 18.189947005631595 94-95 18.15987010140022 31.6927468383456 31.959196001823216 18.18818705843097 96-97 18.17712644594446 31.705582115856533 31.907614679096746 18.20967675910226 98-99 18.20348005191391 31.711493298733252 31.883028768685374 18.201997880667463 100-101 18.24609569353088 31.730379975676016 31.84358543201098 18.17993889878213 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 8115.0 1 8196.5 2 9667.0 3 13099.5 4 17852.5 5 23686.0 6 30404.5 7 37609.5 8 45999.5 9 55676.5 10 65376.5 11 75027.0 12 85223.0 13 95407.5 14 104177.5 15 112640.5 16 120988.5 17 129470.0 18 137699.5 19 145525.0 20 153711.0 21 162241.5 22 172127.5 23 181923.0 24 191714.0 25 203430.0 26 216364.5 27 230463.5 28 245487.0 29 261818.0 30 279117.5 31 295689.0 32 311724.0 33 326442.5 34 339724.0 35 350511.5 36 360699.5 37 370193.5 38 374968.5 39 373326.0 40 365177.0 41 353336.5 42 336356.5 43 315742.5 44 297061.0 45 280826.5 46 266506.5 47 255519.0 48 243590.5 49 226920.5 50 209588.0 51 191702.5 52 174411.5 53 159776.0 54 146700.0 55 134868.0 56 123768.5 57 112829.5 58 101445.0 59 89029.5 60 77041.5 61 65973.0 62 55319.5 63 46472.5 64 39433.0 65 33630.5 66 28942.0 67 24584.0 68 20826.0 69 17839.5 70 15183.0 71 12863.5 72 10760.5 73 9008.5 74 7620.5 75 6401.5 76 5293.5 77 4540.0 78 3840.0 79 3132.5 80 2560.0 81 2095.5 82 1754.0 83 1417.5 84 1137.5 85 882.0 86 630.5 87 447.0 88 292.0 89 181.5 90 123.5 91 70.5 92 37.5 93 23.0 94 10.5 95 11.0 96 9.5 97 3.0 98 2.0 99 1.5 100 1.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.08214554194342927 2 1.292544979703597E-4 3 0.0 4 0.0 5 0.002688493557783482 6 0.0 7 0.0 8 1.5510539756443165E-4 9 0.0034554035790742826 10-11 0.0 12-13 9.047814857925179E-5 14-15 2.800514122691127E-4 16-17 0.0015596709421756736 18-19 0.0 20-21 9.909511511060911E-5 22-23 1.7664781389282493E-4 24-25 3.877634939110791E-5 26-27 0.003317532114572566 28-29 0.0016156812246294963 30-31 0.002623866308798302 32-33 0.0029125346875987722 34-35 0.004928904855936384 36-37 0.006471341865049342 38-39 0.0082765963533687 40-41 0.01023264775598681 42-43 0.018681583439982656 44-45 0.011189131040967472 46-47 0.014032729996315385 48-49 0.01488580968291976 50-51 0.011826786564287912 52-53 0.016678138721442082 54-55 0.013127948510522867 56-57 0.010947855978089466 58-59 0.008104257022741554 60-61 0.004859969123685525 62-63 0.0018526478042418222 64-65 0.0016759999903489975 66-67 0.0019388174695553957 68-69 0.010021532075968556 70-71 0.005023691487781314 72-73 0.0014562673437993861 74-75 0.021473480596142427 76-77 0.034136112913972 78-79 0.03762598435917171 80-81 0.025329573118924822 82-83 0.044967639843888144 84-85 0.02007753201806254 86-87 0.01704435979902477 88-89 0.0173201027280282 90-91 0.0059974087058246904 92-93 0.01793190735175457 94-95 0.02088321838874445 96-97 0.012382580905560459 98-99 0.003576041110513285 100-101 5.1701799188143884E-5 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1.1605012E7 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 94.5022741640138 #Duplication Level Percentage of deduplicated Percentage of total 1 97.34537108345052 91.99358946725901 2 2.0689395417670173 3.910389836096715 3 0.23804321848509294 0.6748687648848746 4 0.12236997584667957 0.4625696402762664 5 0.05293500382490448 0.2501239122167121 6 0.0330957909596476 0.18765765065661041 7 0.01891840408701514 0.1251482546843693 8 0.008279656492132954 0.06259570942427241 9 0.001472446551477629 0.0125234592929636 >10 0.1090957902912582 2.152912533080116 >50 0.0014575255998759684 0.076351895467864 >100 0.0 0.0 >500 0.0 0.0 >1k 1.2404086694567754E-5 0.022565127230111573 >5k 9.158557670966807E-6 0.06870374943015038 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 4.308483265678657E-5 0.0 8.616966531357313E-6 2 4.308483265678657E-5 0.0 8.616966531357313E-6 3 5.1701799188143884E-5 0.0 1.7233933062714626E-5 4 5.1701799188143884E-5 8.616966531357313E-6 2.5850899594071942E-5 5 6.0318765719501194E-5 8.616966531357313E-6 3.446786612542925E-5 6 6.0318765719501194E-5 8.616966531357313E-6 3.446786612542925E-5 7 8.616966531357314E-5 8.616966531357313E-6 3.446786612542925E-5 8 9.478663184493045E-5 2.5850899594071942E-5 5.1701799188143884E-5 9 9.478663184493045E-5 2.5850899594071942E-5 6.0318765719501194E-5 10-11 1.0771208164196642E-4 3.0159382859750597E-5 8.186118204789448E-5 12-13 1.6372236409578896E-4 4.308483265678657E-5 1.2494601470468104E-4 14-15 2.0680719675257554E-4 4.308483265678657E-5 1.4648843103307434E-4 16-17 2.628174792063981E-4 4.308483265678657E-5 1.8095629715850358E-4 18-19 3.5329562778564985E-4 4.308483265678657E-5 2.1542416328393285E-4 20-21 4.954755755530455E-4 4.308483265678657E-5 2.4127506287800477E-4 22-23 7.065912555712997E-4 4.308483265678657E-5 2.5420051267504077E-4 24-25 9.004730025268394E-4 6.0318765719501194E-5 2.585089959407194E-4 26-27 0.0010857377829510215 6.462724898517985E-5 2.671259624720767E-4 28-29 0.0013097789127663117 6.89357322508585E-5 2.75742929003434E-4 30-31 0.0014864267266591366 6.89357322508585E-5 2.8005141226911264E-4 32-33 0.0016975424066773908 7.755269878221583E-5 2.9728534533182737E-4 34-35 0.0018871156703672517 8.186118204789448E-5 3.059023118631846E-4 36-37 0.00208530590058847 8.616966531357314E-5 3.102107951288633E-4 38-39 0.002283496130809688 8.616966531357314E-5 3.14519278394542E-4 40-41 0.002520462710422014 8.616966531357314E-5 3.2313624492589924E-4 42-43 0.002710035974111875 8.616966531357314E-5 3.274447281915779E-4 44-45 0.0029340771039271653 8.616966531357314E-5 3.274447281915779E-4 46-47 0.0031839691333365276 8.616966531357314E-5 3.4037017798861386E-4 48-49 0.0034209357129488535 9.478663184493045E-5 3.7052956084836447E-4 50-51 0.0037526889244061104 9.478663184493045E-5 3.920719771767577E-4 52-53 0.004058591236269294 1.0340359837628777E-4 4.222313600365084E-4 54-55 0.00452390742896259 1.2063753143900239E-4 4.480822596305803E-4 56-57 0.004928904855936384 1.2063753143900239E-4 4.782416424903309E-4 58-59 0.00542007194822375 1.2063753143900239E-4 5.084010253500815E-4 60-61 0.0059887917392933325 1.2063753143900239E-4 5.342519249441534E-4 62-63 0.006850488392429064 1.2063753143900239E-4 5.342519249441534E-4 64-65 0.00804824674028773 1.2063753143900239E-4 5.342519249441534E-4 66-67 0.009633768582057476 1.2063753143900239E-4 5.38560408209832E-4 68-69 0.011861254430413342 1.2063753143900239E-4 5.51485858006868E-4 70-71 0.015351125875613054 1.2063753143900239E-4 5.687197910695828E-4 72-73 0.020012904769077362 1.2063753143900239E-4 5.859537241322974E-4 74-75 0.026527331466783488 1.292544979703597E-4 5.859537241322974E-4 76-77 0.03578195352146124 1.292544979703597E-4 5.945706906636547E-4 78-79 0.048944369898109547 1.292544979703597E-4 6.118046237263692E-4 80-81 0.06734590192582308 1.292544979703597E-4 6.333470400547625E-4 82-83 0.09171037479323588 1.292544979703597E-4 6.678149061801917E-4 84-85 0.12401107383602877 1.4648843103307432E-4 6.936658057742637E-4 86-87 0.16616096562416308 1.5510539756443165E-4 7.238251886340143E-4 88-89 0.21965940233409498 1.5510539756443165E-4 7.410591216967289E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATGCCG 1765 0.0 24.35711 46-47 CTCGTAT 1915 0.0 21.705019 42-43 TCTCGTA 1845 0.0 21.242226 40-41 GCCGTCT 2095 0.0 20.973104 48-49 ATGCCGT 2145 0.0 20.927935 46-47 TGCCGTC 2130 0.0 19.959442 48-49 CGTATGC 2315 0.0 18.568842 44-45 TCACGAT 2175 0.0 18.560425 34-35 CGTCTGA 2395 0.0 18.441029 16-17 ATCTCGT 2235 0.0 17.322975 40-41 ACACGTC 2725 0.0 16.731031 12-13 ACGTCTG 2920 0.0 15.857684 14-15 ACGATCT 2530 0.0 15.583715 36-37 CACGATC 2435 0.0 15.508921 36-37 CATCACG 2945 0.0 15.400709 32-33 CGGAAGA 5990 0.0 14.985064 4 TCGGAAG 6050 0.0 14.836452 3 CACGTCT 3175 0.0 14.210123 14-15 CGTCTTC 3265 0.0 14.039268 50-51 TCGTATG 3280 0.0 13.686074 42-43 >>END_MODULE