FastQCFastQC Report
Fri 3 Apr 2015
H2K5YBCXX l01n01 64249-bar7.3410000000446d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2K5YBCXX l01n01 64249-bar7.3410000000446d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11605012
Sequences flagged as poor quality0
Sequence length101
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG17650.024.3571146-47
CTCGTAT19150.021.70501942-43
TCTCGTA18450.021.24222640-41
GCCGTCT20950.020.97310448-49
ATGCCGT21450.020.92793546-47
TGCCGTC21300.019.95944248-49
CGTATGC23150.018.56884244-45
TCACGAT21750.018.56042534-35
CGTCTGA23950.018.44102916-17
ATCTCGT22350.017.32297540-41
ACACGTC27250.016.73103112-13
ACGTCTG29200.015.85768414-15
ACGATCT25300.015.58371536-37
CACGATC24350.015.50892136-37
CATCACG29450.015.40070932-33
CGGAAGA59900.014.9850644
TCGGAAG60500.014.8364523
CACGTCT31750.014.21012314-15
CGTCTTC32650.014.03926850-51
TCGTATG32800.013.68607442-43
Produced by FastQC (version 0.11.2)