##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename H2K5YBCXX l01n01 61495-bar5.3410000000441d.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 15526190 Sequences flagged as poor quality 0 Sequence length 101 %GC 40 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.989474107942776 38.0 38.0 38.0 32.0 38.0 2 36.967905068790216 38.0 38.0 38.0 32.0 38.0 3 36.94975902008155 38.0 38.0 38.0 32.0 38.0 4 36.94445759069031 38.0 38.0 38.0 32.0 38.0 5 36.94260182311307 38.0 38.0 38.0 32.0 38.0 6 38.88710778368679 40.0 38.0 40.0 38.0 40.0 7 38.84616361129163 40.0 38.0 40.0 38.0 40.0 8 38.836993557337635 40.0 38.0 40.0 38.0 40.0 9 38.85394092175866 40.0 38.0 40.0 38.0 40.0 10-11 38.85312581515491 40.0 38.0 40.0 38.0 40.0 12-13 38.84252888184416 40.0 38.0 40.0 38.0 40.0 14-15 38.833080073089405 40.0 38.0 40.0 38.0 40.0 16-17 38.82653600142727 40.0 38.0 40.0 38.0 40.0 18-19 38.791928122739705 40.0 38.0 40.0 38.0 40.0 20-21 38.77771884151875 40.0 38.0 40.0 38.0 40.0 22-23 38.76207765717153 40.0 38.0 40.0 38.0 40.0 24-25 38.73222335936891 40.0 38.0 40.0 38.0 40.0 26-27 38.6706285637365 40.0 38.0 40.0 38.0 40.0 28-29 38.620296898337585 40.0 38.0 40.0 38.0 40.0 30-31 38.60409981457138 40.0 38.0 40.0 38.0 40.0 32-33 38.56702413792437 40.0 38.0 40.0 38.0 40.0 34-35 38.545092839904704 40.0 38.0 40.0 38.0 40.0 36-37 38.51651789653482 40.0 38.0 40.0 38.0 40.0 38-39 38.48915010701273 40.0 38.0 40.0 38.0 40.0 40-41 38.45557403329471 40.0 38.0 40.0 38.0 40.0 42-43 38.40294083738509 40.0 38.0 40.0 38.0 40.0 44-45 38.382119502595295 40.0 38.0 40.0 38.0 40.0 46-47 38.347233287754435 40.0 38.0 40.0 38.0 40.0 48-49 38.284303554188114 40.0 38.0 40.0 38.0 40.0 50-51 38.271651094054626 40.0 38.0 40.0 38.0 40.0 52-53 38.245461925945776 40.0 38.0 40.0 38.0 40.0 54-55 38.18053022022788 40.0 38.0 40.0 38.0 40.0 56-57 38.17179211384119 40.0 38.0 40.0 38.0 40.0 58-59 38.14284499287977 40.0 38.0 40.0 38.0 40.0 60-61 38.08599740180946 40.0 38.0 40.0 38.0 40.0 62-63 38.06131478488928 40.0 38.0 40.0 38.0 40.0 64-65 37.998639427960114 40.0 38.0 40.0 38.0 40.0 66-67 37.94655053815521 40.0 38.0 40.0 38.0 40.0 68-69 37.905271351181455 40.0 38.0 40.0 38.0 40.0 70-71 37.885873224532226 40.0 38.0 40.0 38.0 40.0 72-73 37.85745459768302 40.0 38.0 40.0 38.0 40.0 74-75 37.78119026625335 40.0 38.0 40.0 32.0 40.0 76-77 37.72061896704858 40.0 38.0 40.0 32.0 40.0 78-79 37.64651559719416 40.0 38.0 40.0 32.0 40.0 80-81 37.61395938089126 40.0 38.0 40.0 32.0 40.0 82-83 37.54118627944138 40.0 38.0 40.0 32.0 40.0 84-85 37.50582180818346 40.0 38.0 40.0 32.0 40.0 86-87 37.46718039003774 40.0 38.0 40.0 32.0 40.0 88-89 37.400490203971486 40.0 38.0 40.0 32.0 40.0 90-91 37.34360941093726 38.0 38.0 40.0 32.0 40.0 92-93 37.264906973314126 38.0 38.0 40.0 32.0 40.0 94-95 37.193629989070075 38.0 38.0 40.0 32.0 40.0 96-97 37.11320104932375 38.0 38.0 40.0 32.0 40.0 98-99 37.09311659847007 38.0 38.0 40.0 32.0 40.0 100-101 36.27331673127792 38.0 38.0 40.0 29.5 40.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2201 1 0.11344863194757693 2201 2 0.09517434281092818 2201 3 0.06654248218413983 2201 4 0.10041838201944842 2201 5 0.030629322462822017 2201 6 0.04122713678500389 2201 7 0.057376802431853946 2201 8 0.0049210479093133586 2201 9 -7.64736084732931E-4 2201 10-11 0.02627026174633329 2201 12-13 0.04199907026684713 2201 14-15 -0.0015490298088138843 2201 16-17 -0.1251644218538459 2201 18-19 -0.06748679087426979 2201 20-21 -0.08655739271548413 2201 22-23 -0.10316890564852343 2201 24-25 -0.09399087950811236 2201 26-27 -0.10402007647422096 2201 28-29 -0.1539357284931242 2201 30-31 -0.13001531939791278 2201 32-33 -0.1536365771752557 2201 34-35 -0.16575301847665713 2201 36-37 -0.1872588866293583 2201 38-39 -0.16496074650381587 2201 40-41 -0.18142690261322514 2201 42-43 -0.12885580534424435 2201 44-45 -0.24667715193590567 2201 46-47 -0.21337891450420443 2201 48-49 -0.19325027848821463 2201 50-51 -0.3004762757700945 2201 52-53 -0.2024745065424014 2201 54-55 -0.16850507237830925 2201 56-57 -0.29642916430324817 2201 58-59 -0.2243168029561815 2201 60-61 -0.23402093446542693 2201 62-63 -0.21726100556762873 2201 64-65 -0.2814997226507714 2201 66-67 -0.2090300113361252 2201 68-69 -0.2970297771156325 2201 70-71 -0.333888802818322 2201 72-73 -0.25507316770866595 2201 74-75 -0.251826150657358 2201 76-77 -0.2665613045480484 2201 78-79 -0.291581397230928 2201 80-81 -0.3009295035490567 2201 82-83 -0.331325160298654 2201 84-85 -0.3923569574887438 2201 86-87 -0.42248674233171357 2201 88-89 -0.45180279872736406 2201 90-91 -0.40651156397292 2201 92-93 -0.5117785251019242 2201 94-95 -0.4436428115484361 2201 96-97 -0.41600531878127356 2201 98-99 -0.4304141712079783 2201 100-101 -0.46461005986135717 1101 1 0.116402899791197 1101 2 0.14219348687871758 1101 3 0.07306113170427864 1101 4 0.09027030660605817 1101 5 0.11547321956268775 1101 6 0.07858551726312868 1101 7 0.05864603406093494 1101 8 0.07829622359539457 1101 9 0.05858101845532104 1101 10-11 0.0645583582222713 1101 12-13 0.07819299596500429 1101 14-15 0.06406822922789956 1101 16-17 -0.001600650139359061 1101 18-19 -0.04227017155248802 1101 20-21 -0.01121747458287814 1101 22-23 -0.03590271591757954 1101 24-25 0.025077706628415797 1101 26-27 0.030786795102265785 1101 28-29 -0.021431012787409998 1101 30-31 -0.06614718628928529 1101 32-33 -0.07645497850617033 1101 34-35 -0.022446017777731697 1101 36-37 -0.05764846146741576 1101 38-39 -0.017857371555365376 1101 40-41 -0.004792940368858467 1101 42-43 5.259507557653365E-4 1101 44-45 -0.055848800495837736 1101 46-47 -0.015361438309973607 1101 48-49 -0.021505520734685035 1101 50-51 -0.1261478849079012 1101 52-53 -0.0586793476169305 1101 54-55 -0.14296516397933345 1101 56-57 -0.0714970812295519 1101 58-59 -0.10793379086798893 1101 60-61 -0.08085883102930325 1101 62-63 -0.1399722863416244 1101 64-65 -0.08639866537404828 1101 66-67 -0.135323222845102 1101 68-69 -0.1757243323258919 1101 70-71 -0.17686245880653928 1101 72-73 -0.12698559832089273 1101 74-75 -0.09896452942243883 1101 76-77 -0.10501873218819924 1101 78-79 -0.15427809273632676 1101 80-81 -0.11273445618316202 1101 82-83 -0.1958280587223058 1101 84-85 -0.1488535407353595 1101 86-87 -0.22093488346092727 1101 88-89 -0.2377537079157932 1101 90-91 -0.24175320829807845 1101 92-93 -0.24312800675839696 1101 94-95 -0.2217995306666225 1101 96-97 -0.2560708519796364 1101 98-99 -0.287789267681795 1101 100-101 -0.2926882866180094 2202 1 0.015826799795704005 2202 2 0.030439298214503197 2202 3 0.038376817623522186 2202 4 0.07720932349916154 2202 5 0.05021171397221735 2202 6 -0.03572996396479766 2202 7 -0.007650087438165087 2202 8 -0.022099283946403148 2202 9 -0.01398911252163515 2202 10-11 0.02965973941122968 2202 12-13 -0.017788415755234155 2202 14-15 -0.017782531653878664 2202 16-17 -0.03703286103511516 2202 18-19 -0.011813227013647065 2202 20-21 -0.01576016685412185 2202 22-23 -0.11717233838113117 2202 24-25 -0.04344629650820053 2202 26-27 -0.11367522644687966 2202 28-29 -0.073322616437423 2202 30-31 -0.06837441651837395 2202 32-33 -0.09343746086963733 2202 34-35 -0.09781905875060204 2202 36-37 -0.04772200925982162 2202 38-39 -0.07357591221208537 2202 40-41 -0.15186261250797628 2202 42-43 -0.12930446958998942 2202 44-45 -0.150480451824194 2202 46-47 -0.127096484424726 2202 48-49 -0.10608491484750004 2202 50-51 -0.22702968234403897 2202 52-53 -0.18272932514064166 2202 54-55 -0.19011805651578584 2202 56-57 -0.15419471411719599 2202 58-59 -0.20415351004462678 2202 60-61 -0.11835118751300655 2202 62-63 -0.17953745468824422 2202 64-65 -0.19298761948348186 2202 66-67 -0.1410803780859453 2202 68-69 -0.19856185187840225 2202 70-71 -0.2596378912499091 2202 72-73 -0.24548242379048446 2202 74-75 -0.27644673041635315 2202 76-77 -0.24173761732438948 2202 78-79 -0.22163039684030394 2202 80-81 -0.23345002736810017 2202 82-83 -0.31530740363056253 2202 84-85 -0.31539757337396424 2202 86-87 -0.2779241944860047 2202 88-89 -0.2779237149285336 2202 90-91 -0.3912053176363486 2202 92-93 -0.4201170245956689 2202 94-95 -0.3047734439088714 2202 96-97 -0.3453748973707249 2202 98-99 -0.35748125909159256 2202 100-101 -0.3785263368469387 1102 1 0.02270963736167886 1102 2 0.05680893194494274 1102 3 0.0016618639071808161 1102 4 0.0436893648292056 1102 5 0.020960080568606543 1102 6 -0.08316155265166003 1102 7 -0.1143518865120896 1102 8 -0.0629476895443517 1102 9 -0.07876010890547036 1102 10-11 -0.047096259286526276 1102 12-13 -0.053342432220510716 1102 14-15 -0.04117364348024921 1102 16-17 -0.04728353325194945 1102 18-19 -0.042660026369375714 1102 20-21 -0.07173860473585592 1102 22-23 -0.18697797600116672 1102 24-25 -0.10889816080909753 1102 26-27 -0.10672763626721604 1102 28-29 -0.1830563104141376 1102 30-31 -0.14785044339765818 1102 32-33 -0.13889700904137214 1102 34-35 -0.15961453216006305 1102 36-37 -0.18750330195148734 1102 38-39 -0.18120119125507017 1102 40-41 -0.18842379341105442 1102 42-43 -0.2063885043341358 1102 44-45 -0.222315377403703 1102 46-47 -0.25918702559107487 1102 48-49 -0.24107763543992178 1102 50-51 -0.22972322015431246 1102 52-53 -0.22530314772794924 1102 54-55 -0.32813393568007854 1102 56-57 -0.26374076630805376 1102 58-59 -0.24876816652300704 1102 60-61 -0.22970151588723553 1102 62-63 -0.2944108852843712 1102 64-65 -0.31007133552727595 1102 66-67 -0.3155056308052906 1102 68-69 -0.33892899129012477 1102 70-71 -0.3507284579620773 1102 72-73 -0.34473033029959765 1102 74-75 -0.3639492132152853 1102 76-77 -0.38101576764714906 1102 78-79 -0.41377660123788473 1102 80-81 -0.4762212883146475 1102 82-83 -0.4764717198082451 1102 84-85 -0.4676605882063285 1102 86-87 -0.44405378216877267 1102 88-89 -0.5201685167494006 1102 90-91 -0.4781987062317228 1102 92-93 -0.64454991313184 1102 94-95 -0.595418818201253 1102 96-97 -0.5793462871128767 1102 98-99 -0.576113738534417 1102 100-101 -0.6535231349377213 2203 1 0.06620223273604608 2203 2 0.04461369335967902 2203 3 0.033521480995197805 2203 4 0.01053810716354775 2203 5 0.038501744629620305 2203 6 -0.08947779893283325 2203 7 -0.07151966563534273 2203 8 -0.07310073363680658 2203 9 -0.04995714990896971 2203 10-11 -0.03354036926394599 2203 12-13 -0.06193566105611836 2203 14-15 -0.03813063256225746 2203 16-17 -0.0509743166668315 2203 18-19 -0.031876059509244214 2203 20-21 -0.04444602808156617 2203 22-23 -0.15434549274278453 2203 24-25 -0.08162871609361844 2203 26-27 -0.09086230340651724 2203 28-29 -0.090871712114442 2203 30-31 -0.10521458296153696 2203 32-33 -0.08658731369600758 2203 34-35 -0.1183905325649306 2203 36-37 -0.14822561925926436 2203 38-39 -0.14051511741111256 2203 40-41 -0.18964356794852932 2203 42-43 -0.14867655462847296 2203 44-45 -0.17278185396498458 2203 46-47 -0.15784310788806977 2203 48-49 -0.14967612650212914 2203 50-51 -0.14625531210651133 2203 52-53 -0.16448511412614408 2203 54-55 -0.17221129212562403 2203 56-57 -0.18446508896620628 2203 58-59 -0.2478965107021338 2203 60-61 -0.18253281319282877 2203 62-63 -0.24839599341874674 2203 64-65 -0.26918434067340513 2203 66-67 -0.2550395624403592 2203 68-69 -0.3099770875537047 2203 70-71 -0.2997021200222747 2203 72-73 -0.3120666086650772 2203 74-75 -0.29458296024500186 2203 76-77 -0.33907754227082876 2203 78-79 -0.28892457901523727 2203 80-81 -0.3451754258971391 2203 82-83 -0.3877290593386178 2203 84-85 -0.42668330119032305 2203 86-87 -0.48347672965824984 2203 88-89 -0.4669051251058889 2203 90-91 -0.4668774545378369 2203 92-93 -0.5990443616940411 2203 94-95 -0.5238794498962847 2203 96-97 -0.55008447085455 2203 98-99 -0.49618264644102794 2203 100-101 -0.44752595542941975 1103 1 0.07979231386850927 1103 2 0.08228739607090318 1103 3 0.11397593424950259 1103 4 0.03594783073731378 1103 5 0.09255632010698633 1103 6 -0.0672214120186041 1103 7 -0.008639433390278839 1103 8 -0.03410748351402759 1103 9 -0.001830167831066376 1103 10-11 0.02922613724744849 1103 12-13 0.010142170294244579 1103 14-15 -0.018140328814567397 1103 16-17 -0.003914230737883884 1103 18-19 0.03204148634658566 1103 20-21 0.029024964746113824 1103 22-23 -0.043880749300271304 1103 24-25 -0.029647946561773608 1103 26-27 -0.03720835438171122 1103 28-29 -0.05627465685050481 1103 30-31 -0.028922342928964895 1103 32-33 0.024222456495756717 1103 34-35 -0.01268553485051882 1103 36-37 -0.06656654450332411 1103 38-39 -0.03652212962791879 1103 40-41 -0.04596229810744035 1103 42-43 -0.013087460035038134 1103 44-45 -0.019538564045959106 1103 46-47 -0.017401418454575435 1103 48-49 -0.053735252102569575 1103 50-51 -0.11094225844342986 1103 52-53 -0.07510891187960311 1103 54-55 -0.022239287189016466 1103 56-57 -0.025488204521124658 1103 58-59 -0.05971652149696638 1103 60-61 -0.09317305209153659 1103 62-63 -0.06506980118297889 1103 64-65 -0.14818003310116268 1103 66-67 -0.029435199573683235 1103 68-69 -0.20858315421364892 1103 70-71 -0.12205611495729585 1103 72-73 -0.07056235856067161 1103 74-75 -0.06780731616772329 1103 76-77 -0.09713003357815353 1103 78-79 -0.1090772387792569 1103 80-81 -0.18343760090698424 1103 82-83 -0.21259622140835432 1103 84-85 -0.2787470647171375 1103 86-87 -0.18929084419512066 1103 88-89 -0.25058712263074057 1103 90-91 -0.21199967357416938 1103 92-93 -0.24209471576966735 1103 94-95 -0.16758362464720022 1103 96-97 -0.2161578783845215 1103 98-99 -0.2478194046371769 1103 100-101 -0.26650454594117434 2204 1 0.056414981892672245 2204 2 0.07513408055899617 2204 3 0.07014718498756167 2204 4 0.019815650117493533 2204 5 0.060866018100448116 2204 6 -0.027664613526944493 2204 7 0.027975695896138575 2204 8 0.016700016981538113 2204 9 0.07054413947707872 2204 10-11 0.039696694603009064 2204 12-13 0.02908565844108324 2204 14-15 0.031082261646872666 2204 16-17 0.047076182495089824 2204 18-19 0.03537875797356094 2204 20-21 0.052302842501461555 2204 22-23 -0.02022869202361477 2204 24-25 -0.03548000783298022 2204 26-27 0.041603346861073476 2204 28-29 -0.06086646023108244 2204 30-31 -0.02197437250605816 2204 32-33 -0.0037088169921233316 2204 34-35 -0.01567143831756823 2204 36-37 -0.03174084340955119 2204 38-39 -0.022965390448035805 2204 40-41 0.03526207988713992 2204 42-43 0.013593824229488405 2204 44-45 0.014478697036309995 2204 46-47 0.061233945451789396 2204 48-49 0.01370597165812626 2204 50-51 0.02735322169111498 2204 52-53 0.04595636457985819 2204 54-55 0.02922480914083536 2204 56-57 0.026604505172457493 2204 58-59 0.032414207144682905 2204 60-61 0.034155261688141536 2204 62-63 0.0482523171479059 2204 64-65 0.045293373799076164 2204 66-67 0.07405075396643213 2204 68-69 0.02004475549185969 2204 70-71 0.04675545477746823 2204 72-73 0.07672287180601955 2204 74-75 0.009068220490824785 2204 76-77 0.0266851536142525 2204 78-79 0.03461523662406307 2204 80-81 0.03787172452636156 2204 82-83 -0.05255811954373968 2204 84-85 -0.07812327381078177 2204 86-87 -0.08082307780814801 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0.36498780228733096 1214 88-89 0.40948244983324855 1214 90-91 0.49445449485045145 1214 92-93 0.4908547305662836 1214 94-95 0.4470110647281018 1214 96-97 0.47900189482736977 1214 98-99 0.5006051931915962 1214 100-101 0.6207632151926248 1215 1 -0.12372596325747054 1215 2 -0.0392344517131491 1215 3 -0.04328863653159232 1215 4 -0.034050628529570304 1215 5 -0.05784628448918028 1215 6 0.00417481615206583 1215 7 0.03710189999073776 1215 8 0.03839608018360252 1215 9 -0.004415815617505814 1215 10-11 -0.04496287071548011 1215 12-13 -0.006575617795256505 1215 14-15 0.022929356729918027 1215 16-17 -0.005963635126455813 1215 18-19 0.010997663362516619 1215 20-21 -0.027999610715880863 1215 22-23 -0.009299621789395474 1215 24-25 8.69159114650131E-4 1215 26-27 -0.01169315383024383 1215 28-29 -2.2092123680295117E-4 1215 30-31 0.015981159643921217 1215 32-33 0.042457278192181036 1215 34-35 -0.0039681425478477195 1215 36-37 0.05709346488730915 1215 38-39 0.04170881277591576 1215 40-41 0.02685094168082003 1215 42-43 0.0711636191449827 1215 44-45 0.05141577181299084 1215 46-47 0.03229146157408991 1215 48-49 0.05349196919694066 1215 50-51 0.1095733428989405 1215 52-53 0.10752340232847502 1215 54-55 0.10414420094095789 1215 56-57 0.14197019068539873 1215 58-59 0.08814276366597085 1215 60-61 0.12001400904479453 1215 62-63 0.0748396978143333 1215 64-65 0.17990781107815934 1215 66-67 0.24004014403097074 1215 68-69 0.23002841683683783 1215 70-71 0.19602497559120025 1215 72-73 0.2358750300636956 1215 74-75 0.19856278946527084 1215 76-77 0.26918825860046525 1215 78-79 0.20481813301039864 1215 80-81 0.2366087868403497 1215 82-83 0.3354242043904989 1215 84-85 0.25606971702721637 1215 86-87 0.3272658371217787 1215 88-89 0.33668997921743227 1215 90-91 0.33026646822445116 1215 92-93 0.42288465377823314 1215 94-95 0.3535506583504855 1215 96-97 0.4336890258462418 1215 98-99 0.3722241036041396 1215 100-101 0.49273649452850776 1212 1 -0.08058316111238639 1212 2 -0.06305636063917319 1212 3 -0.07834542279505996 1212 4 -0.03648881628708267 1212 5 -0.032046528730255375 1212 6 -0.1256977424325072 1212 7 -0.1086744461461393 1212 8 -0.2215088968282828 1212 9 -0.12075133304198005 1212 10-11 -0.21266118626530783 1212 12-13 -0.21813609018366975 1212 14-15 -0.10439233369142897 1212 16-17 -0.03052613473494148 1212 18-19 -0.023200052095774026 1212 20-21 -0.04120159412885016 1212 22-23 0.00458240034073043 1212 24-25 0.016514162497863083 1212 26-27 0.017166795399795376 1212 28-29 0.05901743338268517 1212 30-31 0.02772173116210297 1212 32-33 -0.012706298778105918 1212 34-35 -0.019208190313094065 1212 36-37 -0.03895508439775597 1212 38-39 -0.060236055300030955 1212 40-41 -0.13004918469726334 1212 42-43 -0.1324689466721125 1212 44-45 0.019394798974559535 1212 46-47 -0.023563302310805057 1212 48-49 0.014716604791566112 1212 50-51 0.030155018946544487 1212 52-53 -0.06591945859415915 1212 54-55 -0.04766031132911763 1212 56-57 -0.053827007889374556 1212 58-59 0.10322083343096722 1212 60-61 -0.2208169542348486 1212 62-63 -0.16569136966379716 1212 64-65 -0.09061442384238916 1212 66-67 -0.07614621714748182 1212 68-69 0.14297964411004926 1212 70-71 0.05581073855738339 1212 72-73 0.17398694002277892 1212 74-75 0.11564548828036436 1212 76-77 0.10090482592607941 1212 78-79 0.21643065155489438 1212 80-81 0.20448226180675277 1212 82-83 0.18258549507783783 1212 84-85 0.2492132836201293 1212 86-87 0.23358249206160053 1212 88-89 0.26810123324843005 1212 90-91 0.20165956449613986 1212 92-93 0.3168420918714929 1212 94-95 0.25938436275953336 1212 96-97 0.3069721330994355 1212 98-99 0.26799333745648113 1212 100-101 0.29874166086864307 1213 1 -0.20697981716864433 1213 2 -0.13654702269207775 1213 3 -0.13540819524702385 1213 4 -0.17102646845744118 1213 5 -0.14004300143156456 1213 6 -0.024539726045972543 1213 7 -8.616289546026223E-4 1213 8 -0.018916303090719566 1213 9 -0.044624024660450345 1213 10-11 -0.06573458011470734 1213 12-13 -0.1429837394927489 1213 14-15 -0.11604669568797021 1213 16-17 -0.22798609496567934 1213 18-19 -0.29205010858269986 1213 20-21 -0.1540065960644199 1213 22-23 -0.22090854131302962 1213 24-25 -0.27812869893862313 1213 26-27 -0.10276050150917371 1213 28-29 -0.13872311523265068 1213 30-31 -0.13296320762626124 1213 32-33 -0.10664887858714422 1213 34-35 -0.14874656037753198 1213 36-37 -0.051634180077918757 1213 38-39 -0.031614309738863255 1213 40-41 0.016533613736278596 1213 42-43 0.04904624262693602 1213 44-45 0.09959990028178822 1213 46-47 0.08846200311189989 1213 48-49 0.09265872106087158 1213 50-51 0.10832247323575928 1213 52-53 0.1118161343193691 1213 54-55 0.11805995025224547 1213 56-57 0.14261886684738556 1213 58-59 0.1551290405403165 1213 60-61 0.17178614342656573 1213 62-63 0.21196630254576831 1213 64-65 0.17380016359350492 1213 66-67 0.20591566652414173 1213 68-69 0.23993213223130283 1213 70-71 0.22692650224038857 1213 72-73 0.19917415924118842 1213 74-75 0.2243277215698356 1213 76-77 0.25939857337781547 1213 78-79 0.30623572699732193 1213 80-81 0.27739203076508545 1213 82-83 0.2687165157274265 1213 84-85 0.3102327344277285 1213 86-87 0.30641065209221807 1213 88-89 0.3450155716540806 1213 90-91 0.3733457276545664 1213 92-93 0.4153461070977187 1213 94-95 0.3382742451932188 1213 96-97 0.34081041818444646 1213 98-99 0.4356781642671095 1213 100-101 0.15819738638303704 >>END_MODULE >>Per sequence quality scores pass #Quality Count 13 24.0 14 30.0 15 96.0 16 159.0 17 264.0 18 583.0 19 1395.0 20 3110.0 21 6755.0 22 12875.0 23 21669.0 24 32716.0 25 46787.0 26 63441.0 27 83071.0 28 106444.0 29 131868.0 30 162257.0 31 197203.0 32 237168.0 33 290078.0 34 362903.0 35 471955.0 36 662865.0 37 1072182.0 38 2418619.0 39 9139673.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 18.32028671343592 30.609407863579708 29.296258625327447 21.774046797656926 2 20.457199918434448 31.31273524004194 29.64748789816827 18.58257694335535 3 20.2559417345788 29.86533721408794 29.76984694892952 20.108874102403746 4 20.184269289503735 29.51799507799402 29.902384293893093 20.39535133860915 5 20.64119755546926 29.795396180580134 29.876094318339693 19.68731194561091 6 20.480967964452322 29.899756475993144 29.23612940457382 20.383146154980714 7 20.672257649816213 30.068052754732488 29.481959192821932 19.777730402629366 8 19.992352265694425 29.976864218966597 29.84902841763843 20.181755097700545 9 19.96253555325499 29.67736043050814 30.235849017107363 20.124254999129505 10-11 20.128679347605562 29.93198911001347 29.79769344571978 20.14163809666119 12-13 19.9415110402573 29.988909698188444 29.966080512538245 20.103498749016016 14-15 19.965609594018343 29.88404227517703 30.034549814643153 20.115798316161477 16-17 20.044699953310477 29.784895201696614 30.0323612693496 20.13804357564331 18-19 20.01105873366228 29.667548832005792 30.04670495466048 20.274687479671446 20-21 20.107057937170282 29.621244385890982 29.972039148734176 20.299658528204553 22-23 20.17175652659601 29.583099212400437 29.91968911316408 20.32545514783948 24-25 20.214672174308365 29.561246708021105 29.852770340789757 20.371310776880776 26-27 20.281091814802675 29.552326431448222 29.776280762683317 20.39030099106579 28-29 20.300756117082162 29.568142560639615 29.747487250151256 20.383614072126967 30-31 20.389498272412926 29.47171862556535 29.75087572525438 20.387907376767338 32-33 20.425559350391328 29.543250586218612 29.67268371349893 20.35850634989113 34-35 20.419479471309902 29.509493846689093 29.67869608866469 20.392330593336315 36-37 20.424357070143913 29.514595700063147 29.652454776242443 20.4085924535505 38-39 20.459718481576488 29.446046553360983 29.672609824797043 20.421625140265494 40-41 20.495031682850907 29.456031857829867 29.61445335886336 20.434483100455864 42-43 20.470241784354663 29.448258993274763 29.62933652488965 20.45216269748093 44-45 20.45239329075345 29.379353791045084 29.650621000486648 20.51763191771482 46-47 20.511119687006033 29.443850423079986 29.588856910720875 20.456172979193106 48-49 20.47727897640177 29.381818060012506 29.618587216172603 20.522315747413124 50-51 20.464258278322305 29.426311198417054 29.579601534661876 20.529828988598766 52-53 20.503680586909848 29.42387234216069 29.606408813542245 20.466038257387215 54-55 20.528146745420035 29.36223118340176 29.617499619627257 20.492122451550948 56-57 20.53265974436316 29.38907737467778 29.582344821615703 20.49591805934336 58-59 20.515376104563014 29.39074002493135 29.611364383104938 20.4825194874007 60-61 20.511304810906744 29.36269291953877 29.532780686037192 20.5932215835173 62-63 20.49154693883821 29.347865397036905 29.615614020494146 20.54497364363074 64-65 20.522723703921166 29.357840739623853 29.574919299603387 20.544516256851594 66-67 20.52183556404861 29.389026211513713 29.53384241428525 20.555295810152423 68-69 20.450252868759584 29.35477982311232 29.586150519038945 20.60881678908915 70-71 20.584298012230462 29.346317375768955 29.51824835101847 20.551136260982116 72-73 20.54999722722299 29.39173903099398 29.51312612026149 20.54513762152154 74-75 20.555823064051005 29.360286207355387 29.53775337249941 20.5461373560942 76-77 20.53300909093633 29.412221206137 29.524653485750196 20.530116217176474 78-79 20.548631264862454 29.392618799367348 29.540984414587406 20.51776552118279 80-81 20.576679128438126 29.36176576991325 29.508606391790014 20.552948709858608 82-83 20.541510322938827 29.441507513520005 29.532098383269677 20.48488378027149 84-85 20.54909144530193 29.38991865070942 29.508310248861356 20.5526796551273 86-87 20.502602463442503 29.401114860179312 29.537681463679625 20.558601212698555 88-89 20.550114874333953 29.378132552065466 29.498946273984224 20.572806299616357 90-91 20.54924630964669 29.331664012392693 29.508916911294648 20.61017276666597 92-93 20.492792923226027 29.361825482499633 29.52798369821929 20.61739789605505 94-95 20.547162845362497 29.34764759923852 29.47926902197388 20.625920533425102 96-97 20.564015091247683 29.39535809505528 29.425758958737408 20.614867854959634 98-99 20.54600674078818 29.394329023310323 29.447975791002396 20.611688444899105 100-101 20.626735939805382 29.373157788023992 29.35601898523345 20.64408728693718 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 711.0 1 644.0 2 606.5 3 715.5 4 864.0 5 978.0 6 1103.5 7 1368.0 8 1680.5 9 1942.0 10 2312.5 11 2845.0 12 3607.0 13 4591.0 14 5826.5 15 7598.5 16 10543.5 17 15258.5 18 22250.5 19 32255.5 20 46424.5 21 64637.5 22 86439.5 23 112129.0 24 142133.0 25 176167.5 26 213874.5 27 254550.0 28 297374.0 29 339985.5 30 380990.5 31 421260.5 32 460035.0 33 496867.5 34 531755.0 35 564970.0 36 595983.0 37 620045.5 38 632932.5 39 632552.5 40 620954.5 41 601123.0 42 572240.5 43 538517.5 44 506773.5 45 479333.5 46 455996.5 47 438696.0 48 420235.0 49 395321.5 50 367574.5 51 338423.5 52 308290.0 53 281124.0 54 258204.0 55 236726.0 56 216784.0 57 197753.5 58 178071.0 59 156293.0 60 133671.0 61 111879.0 62 92717.0 63 77016.5 64 63634.0 65 52290.0 66 43075.5 67 35330.5 68 28855.0 69 23250.0 70 18345.0 71 14580.0 72 11807.5 73 9642.5 74 8086.5 75 6912.5 76 5875.5 77 5232.5 78 4763.5 79 4144.0 80 3500.0 81 3008.5 82 2606.0 83 2220.0 84 1895.5 85 1507.5 86 1114.0 87 775.0 88 534.0 89 394.5 90 266.5 91 162.5 92 90.5 93 51.5 94 27.5 95 18.0 96 12.5 97 4.5 98 2.0 99 2.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.08193252819912676 2 1.8034044411410657E-4 3 0.0 4 0.0 5 0.0027630732330339897 6 0.0 7 0.0 8 1.4813679337944468E-4 9 0.0034586720889026863 10-11 0.0 12-13 7.728876176318852E-5 14-15 3.7034198344861166E-4 16-17 0.0014362828227659202 18-19 0.0 20-21 1.127127775713166E-4 22-23 1.674589838202418E-4 24-25 2.8983285661195694E-5 26-27 0.0030915504705275407 28-29 0.0014266217275455215 30-31 0.0022188315356182035 32-33 0.0026535808205361395 34-35 0.004421561245869077 36-37 0.005896488449516591 38-39 0.007516332081470084 40-41 0.00944533076047633 42-43 0.017576752570978457 44-45 0.01059500109170376 46-47 0.013248581912239899 48-49 0.014224352529500155 50-51 0.011129581693899146 52-53 0.015734703748955796 54-55 0.012520779405636541 56-57 0.010234320203475547 58-59 0.0074648062402946245 60-61 0.004353933579326287 62-63 0.0015876399812188309 64-65 0.0015715381558514998 66-67 0.0019515412345205102 68-69 0.00989296150568813 70-71 0.004981904768652193 72-73 0.001539334505116838 74-75 0.02137356299259509 76-77 0.03408112357249267 78-79 0.03701809651949384 80-81 0.02523800108075452 82-83 0.044866126203530934 84-85 0.020153044629751406 86-87 0.01693267955628522 88-89 0.01742217504745208 90-91 0.0058932680844431245 92-93 0.01759929512649272 94-95 0.02100322100914648 96-97 0.012623831087987458 98-99 0.0035907070569148 100-101 1.1593314264478278E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1.552619E7 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 94.20610324174065 #Duplication Level Percentage of deduplicated Percentage of total 1 98.28857530280123 92.59383672459293 2 1.068052703588616 2.0123416652377877 3 0.23888081392168647 0.6751209185633229 4 0.08296611123501496 0.3126365616228623 5 0.06904559924189227 0.32522584252847764 6 0.04205783791268573 0.237726301231612 7 0.036058866984813094 0.23778757421660476 8 0.02325447171555547 0.17525705306142053 9 0.019199113498017503 0.162780630150972 >10 0.12108233733192048 2.605022886099607 >50 0.010582068303434763 0.6222492582890489 >100 2.4477346512309504E-4 0.040014584405396514 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 5.7966571322391394E-5 0.0 0.0 2 7.084803161625615E-5 6.4407301469323765E-6 0.0 3 7.728876176318852E-5 1.932219044079713E-5 0.0 4 9.017022205705327E-5 1.932219044079713E-5 2.5762920587729506E-5 5 1.2237387279171515E-4 1.932219044079713E-5 3.2203650734661885E-5 6 1.2237387279171515E-4 1.932219044079713E-5 4.5085111028526636E-5 7 1.416960632325123E-4 2.5762920587729506E-5 4.5085111028526636E-5 8 1.5457752352637704E-4 2.5762920587729506E-5 8.37294919101209E-5 9 1.6101825367330943E-4 2.5762920587729506E-5 9.661095220398565E-5 10-11 2.1254409484876844E-4 3.542401580812807E-5 1.0305168235091802E-4 12-13 2.7051066617115986E-4 4.5085111028526636E-5 1.2559423786518136E-4 14-15 3.41358697787416E-4 4.5085111028526636E-5 1.4813679337944465E-4 16-17 3.961049040363412E-4 4.5085111028526636E-5 1.674589838202418E-4 18-19 4.476307452118002E-4 5.7966571322391394E-5 1.7389971396717418E-4 20-21 5.152584117545902E-4 6.440730146932377E-5 1.8678117426103893E-4 22-23 5.98987903664711E-4 6.440730146932377E-5 1.932219044079713E-4 24-25 6.794970305013658E-4 6.440730146932377E-5 2.1576445992223462E-4 26-27 7.439043319706895E-4 6.440730146932377E-5 2.3186628528956558E-4 28-29 8.856003952032018E-4 6.440730146932377E-5 2.576292058772951E-4 30-31 9.757706172602551E-4 6.440730146932377E-5 2.89832856611957E-4 32-33 0.0010723815694642408 6.440730146932377E-5 3.0593468197928793E-4 34-35 0.0011850943470355572 7.084803161625615E-5 3.2525687242008507E-4 36-37 0.0012720442040191444 8.37294919101209E-5 3.349179676404836E-4 38-39 0.0014234013624720553 8.694985698358708E-5 3.5424015808128073E-4 40-41 0.0015650974257045676 9.339058713051947E-5 3.896641738894088E-4 42-43 0.0017100138540105461 9.661095220398565E-5 4.154270944771383E-4 44-45 0.0018581506473899908 9.661095220398565E-5 4.411900150648678E-4 46-47 0.0020835762025326237 9.661095220398565E-5 4.5729184043219874E-4 48-49 0.0022832388370875277 1.0305168235091804E-4 4.894954911668606E-4 50-51 0.002579512423846417 1.2237387279171515E-4 5.023769514607254E-4 52-53 0.0028017176139155837 1.2881460293864754E-4 5.152584117545902E-4 54-55 0.0031076522958948718 1.3525533308557993E-4 5.313602371219211E-4 56-57 0.003506977565004679 1.449164283059785E-4 5.539027926361843E-4 58-59 0.004015795246612337 1.4813679337944468E-4 5.764453481504478E-4 60-61 0.004576138769395454 1.4813679337944468E-4 6.022082687381772E-4 62-63 0.00527173762526415 1.5457752352637707E-4 6.311915543993729E-4 64-65 0.0061669991156877504 1.6101825367330943E-4 6.569544749871025E-4 66-67 0.007329550907209045 1.6101825367330943E-4 6.82717395574832E-4 68-69 0.008637019127036318 1.6101825367330943E-4 6.988192209421628E-4 70-71 0.01035025334612033 1.642386187467756E-4 7.117006812360277E-4 72-73 0.012594847802326264 1.674589838202418E-4 7.278025066033586E-4 74-75 0.015457752352637705 1.70679348893708E-4 7.567857922645544E-4 76-77 0.019518632710278566 1.7712007904064036E-4 7.728876176318852E-4 78-79 0.0251156272079628 1.8034044411410655E-4 7.793283477788176E-4 80-81 0.032805859003400065 1.8356080918757273E-4 7.922098080726823E-4 82-83 0.042779329635924845 1.8678117426103893E-4 8.083116334400133E-4 84-85 0.05616316688125032 1.964422694814375E-4 8.501763793950737E-4 86-87 0.07343720513532295 2.0288299962836987E-4 8.598374746154724E-4 88-89 0.09563196122165193 2.1254409484876844E-4 8.952614904236004E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GATCGGA 3675 0.0 9.952859 94-95 AGATCGG 4375 0.0 9.337591 94-95 ATCGGAA 3820 0.0 8.580255 94-95 TAGATCG 1885 0.0 8.064048 94-95 TCGGAAG 3790 0.0 7.0188065 94-95 CGGAAGA 4090 0.0 5.923267 94-95 AAGATCG 3190 0.0 5.360759 94-95 ACACGTC 2180 0.0 5.1206584 94-95 >>END_MODULE