##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename H2K5YBCXX l01n01 61439-bar3.341000000043d8.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 15107746 Sequences flagged as poor quality 0 Sequence length 101 %GC 40 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.993408480656214 38.0 38.0 38.0 32.0 38.0 2 36.975355556017426 38.0 38.0 38.0 32.0 38.0 3 36.95705911391415 38.0 38.0 38.0 32.0 38.0 4 36.952926995198354 38.0 38.0 38.0 32.0 38.0 5 36.95121972529853 38.0 38.0 38.0 32.0 38.0 6 38.8959750845692 40.0 38.0 40.0 38.0 40.0 7 38.854383241550394 40.0 38.0 40.0 38.0 40.0 8 38.843970305034254 40.0 38.0 40.0 38.0 40.0 9 38.86222656907258 40.0 38.0 40.0 38.0 40.0 10-11 38.86012632195431 40.0 38.0 40.0 38.0 40.0 12-13 38.84853988146213 40.0 38.0 40.0 38.0 40.0 14-15 38.84093858210219 40.0 38.0 40.0 38.0 40.0 16-17 38.83360251092387 40.0 38.0 40.0 38.0 40.0 18-19 38.80073149230864 40.0 38.0 40.0 38.0 40.0 20-21 38.785352196151564 40.0 38.0 40.0 38.0 40.0 22-23 38.77036538077884 40.0 38.0 40.0 38.0 40.0 24-25 38.73991020897492 40.0 38.0 40.0 38.0 40.0 26-27 38.67907989054092 40.0 38.0 40.0 38.0 40.0 28-29 38.63079525562582 40.0 38.0 40.0 38.0 40.0 30-31 38.61465012715993 40.0 38.0 40.0 38.0 40.0 32-33 38.5787740606706 40.0 38.0 40.0 38.0 40.0 34-35 38.557459895076335 40.0 38.0 40.0 38.0 40.0 36-37 38.53081760177858 40.0 38.0 40.0 38.0 40.0 38-39 38.501825917645164 40.0 38.0 40.0 38.0 40.0 40-41 38.468624042262825 40.0 38.0 40.0 38.0 40.0 42-43 38.41562877744966 40.0 38.0 40.0 38.0 40.0 44-45 38.39785858856774 40.0 38.0 40.0 38.0 40.0 46-47 38.3634858568578 40.0 38.0 40.0 38.0 40.0 48-49 38.302739468879075 40.0 38.0 40.0 38.0 40.0 50-51 38.28917219021289 40.0 38.0 40.0 38.0 40.0 52-53 38.26393454059924 40.0 38.0 40.0 38.0 40.0 54-55 38.20040547411904 40.0 38.0 40.0 38.0 40.0 56-57 38.19276363264248 40.0 38.0 40.0 38.0 40.0 58-59 38.164633129257005 40.0 38.0 40.0 38.0 40.0 60-61 38.10995468814474 40.0 38.0 40.0 38.0 40.0 62-63 38.085935023000786 40.0 38.0 40.0 38.0 40.0 64-65 38.02510536647889 40.0 38.0 40.0 38.0 40.0 66-67 37.9719749392133 40.0 38.0 40.0 38.0 40.0 68-69 37.93137007333854 40.0 38.0 40.0 38.0 40.0 70-71 37.912165620205684 40.0 38.0 40.0 38.0 40.0 72-73 37.88394943229784 40.0 38.0 40.0 38.0 40.0 74-75 37.80568189986779 40.0 38.0 40.0 32.0 40.0 76-77 37.74648061332246 40.0 38.0 40.0 32.0 40.0 78-79 37.673316654913314 40.0 38.0 40.0 32.0 40.0 80-81 37.641255684335704 40.0 38.0 40.0 32.0 40.0 82-83 37.57006630903114 40.0 38.0 40.0 32.0 40.0 84-85 37.538027711082776 40.0 38.0 40.0 32.0 40.0 86-87 37.50068226590518 40.0 38.0 40.0 32.0 40.0 88-89 37.43540780338775 40.0 38.0 40.0 32.0 40.0 90-91 37.37888395131875 39.0 38.0 40.0 32.0 40.0 92-93 37.30068803777877 38.0 38.0 40.0 32.0 40.0 94-95 37.22834829232633 38.0 38.0 40.0 32.0 40.0 96-97 37.14829783344253 38.0 38.0 40.0 32.0 40.0 98-99 37.13070665869019 38.0 38.0 40.0 32.0 40.0 100-101 36.31157242119374 38.0 38.0 40.0 29.5 40.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2201 1 0.10329596725923551 2201 2 0.10398143431055473 2201 3 0.01310543737287162 2201 4 0.05574975526450743 2201 5 0.05045844422508594 2201 6 0.019844856091168595 2201 7 0.04106466471662884 2201 8 -0.015873013837570227 2201 9 -0.010731700513680664 2201 10-11 -0.011821952569704308 2201 12-13 -0.0011636897236257937 2201 14-15 -0.033547114340869655 2201 16-17 -0.13719609445046643 2201 18-19 -0.0715262417763114 2201 20-21 -0.06316179934572119 2201 22-23 -0.10319802019926527 2201 24-25 -0.09217180929362456 2201 26-27 -0.1289979280664184 2201 28-29 -0.13000380273242484 2201 30-31 -0.1234424548110411 2201 32-33 -0.11844297551780159 2201 34-35 -0.14630106210609028 2201 36-37 -0.1487635625285222 2201 38-39 -0.17352664918607275 2201 40-41 -0.23502354501454903 2201 42-43 -0.14089160851349902 2201 44-45 -0.18911010315699883 2201 46-47 -0.16694327543891774 2201 48-49 -0.09715358156539367 2201 50-51 -0.3054522917668052 2201 52-53 -0.1890628412750388 2201 54-55 -0.17038466331462843 2201 56-57 -0.24001685269507078 2201 58-59 -0.2187823257368322 2201 60-61 -0.21884728665883557 2201 62-63 -0.18223818990736618 2201 64-65 -0.27704254811136053 2201 66-67 -0.20921440896337629 2201 68-69 -0.2928919484260888 2201 70-71 -0.30748606631149755 2201 72-73 -0.294115934292833 2201 74-75 -0.24762759632679376 2201 76-77 -0.30569421340376124 2201 78-79 -0.2731589622883064 2201 80-81 -0.25308982378219014 2201 82-83 -0.31281204874439084 2201 84-85 -0.3344897069626356 2201 86-87 -0.4558570135216158 2201 88-89 -0.41078434994846447 2201 90-91 -0.361363650000051 2201 92-93 -0.4480783647945117 2201 94-95 -0.4245275632897929 2201 96-97 -0.43457260114081464 2201 98-99 -0.41756053290261974 2201 100-101 -0.4792165706131115 1101 1 0.10313690844697021 1101 2 0.13486139695000787 1101 3 0.09605559664503005 1101 4 0.030101366353974868 1101 5 0.07636040999616966 1101 6 0.07980305758967177 1101 7 0.09399156800415653 1101 8 0.0991274119885972 1101 9 0.02990541345136677 1101 10-11 0.056601759654796524 1101 12-13 0.07605638906385082 1101 14-15 0.06873218573598905 1101 16-17 0.005001976572849287 1101 18-19 -0.0499569822659538 1101 20-21 -0.04955758351795936 1101 22-23 -0.08305474560361148 1101 24-25 -0.018750312023485094 1101 26-27 0.011781038554296686 1101 28-29 -0.06333330570272011 1101 30-31 -0.0669802824743968 1101 32-33 -0.0985724478841945 1101 34-35 -0.08090176650765812 1101 36-37 -0.07901818121856508 1101 38-39 -0.043033256976812595 1101 40-41 -0.05108727815952108 1101 42-43 -0.03622664568788991 1101 44-45 -0.07109638119224826 1101 46-47 -0.08614591917450554 1101 48-49 -0.08375045614031507 1101 50-51 -0.1629316424892977 1101 52-53 -0.11775871676400129 1101 54-55 -0.15346865610554516 1101 56-57 -0.08823748448359225 1101 58-59 -0.12060600522648457 1101 60-61 -0.1044561701655482 1101 62-63 -0.11122258609086089 1101 64-65 -0.13755456012743394 1101 66-67 -0.14715332474241904 1101 68-69 -0.21235735238468578 1101 70-71 -0.174726212202728 1101 72-73 -0.15246265987735796 1101 74-75 -0.13754105937996286 1101 76-77 -0.1392986223344863 1101 78-79 -0.16454193644403858 1101 80-81 -0.2238418335219876 1101 82-83 -0.25400355716841716 1101 84-85 -0.2307976100256326 1101 86-87 -0.2954807940928674 1101 88-89 -0.28329255310614343 1101 90-91 -0.2839677841247905 1101 92-93 -0.287756370237922 1101 94-95 -0.267337337927664 1101 96-97 -0.30908740008125335 1101 98-99 -0.3118757438399342 1101 100-101 -0.3163072535926901 2202 1 0.08962024024788917 2202 2 0.07986435824462745 2202 3 0.07490144615815808 2202 4 0.07164426116701605 2202 5 0.0699538589256008 2202 6 0.037222574293956256 2202 7 0.04972443000144011 2202 8 0.06819118998208751 2202 9 0.017050209804175154 2202 10-11 0.04648426860259036 2202 12-13 0.038647305598132675 2202 14-15 0.021221522405120652 2202 16-17 -0.0157352055298432 2202 18-19 0.015224818750652958 2202 20-21 0.012543096485771343 2202 22-23 -0.0396149652196911 2202 24-25 0.023561822099857466 2202 26-27 -0.06445664504467175 2202 28-29 -0.02160779041249583 2202 30-31 -0.01524794153748843 2202 32-33 -0.042032952761445586 2202 34-35 -0.031072967206746682 2202 36-37 0.02850707347442949 2202 38-39 -0.040593866511102306 2202 40-41 -0.10716125970566992 2202 42-43 -0.10145394512270656 2202 44-45 -0.049413536169879535 2202 46-47 -0.08038653210336832 2202 48-49 -0.06401570292968728 2202 50-51 -0.13691205419707586 2202 52-53 -0.16203219513367628 2202 54-55 -0.10597081930375651 2202 56-57 -0.13898512498887072 2202 58-59 -0.15478489041819188 2202 60-61 -0.09549582650558364 2202 62-63 -0.08758133912896682 2202 64-65 -0.10123469958676168 2202 66-67 -0.10841090331772563 2202 68-69 -0.14678284467417768 2202 70-71 -0.15502867693469113 2202 72-73 -0.12635644745516572 2202 74-75 -0.199950668971006 2202 76-77 -0.19242297401962816 2202 78-79 -0.20090305471569536 2202 80-81 -0.16868910970608653 2202 82-83 -0.23551962530682147 2202 84-85 -0.30030132338672644 2202 86-87 -0.23606981530859628 2202 88-89 -0.28107220894482765 2202 90-91 -0.2544739066324695 2202 92-93 -0.2859364966491569 2202 94-95 -0.21471603345985812 2202 96-97 -0.26313675172517037 2202 98-99 -0.26954699710563546 2202 100-101 -0.3410556034751693 1102 1 0.04884571193138498 1102 2 0.06666811672577211 1102 3 0.04484251009344575 1102 4 0.0554450768102015 1102 5 0.010815962359167486 1102 6 -0.05126704801650561 1102 7 -0.06931274705899426 1102 8 -0.07808756717977872 1102 9 -0.05745462924981837 1102 10-11 -0.045803656027658235 1102 12-13 -0.04548615871340189 1102 14-15 -0.01849031984992422 1102 16-17 -0.018566368950679646 1102 18-19 -0.029751244010739697 1102 20-21 -0.04319835746132128 1102 22-23 -0.14802416885883218 1102 24-25 -0.08598151245645624 1102 26-27 -0.1344085956249188 1102 28-29 -0.13977553589074887 1102 30-31 -0.14971715417787834 1102 32-33 -0.13283937769175935 1102 34-35 -0.13924568110639512 1102 36-37 -0.150908520054152 1102 38-39 -0.12985110468879668 1102 40-41 -0.17588395602695783 1102 42-43 -0.17182934917973114 1102 44-45 -0.1851052097335426 1102 46-47 -0.19605338366385894 1102 48-49 -0.17626495633843575 1102 50-51 -0.18784762311263137 1102 52-53 -0.18915665537636528 1102 54-55 -0.2899198022247944 1102 56-57 -0.27886892018344867 1102 58-59 -0.26983629993737424 1102 60-61 -0.1981420469929418 1102 62-63 -0.29991106011483737 1102 64-65 -0.29568033474798483 1102 66-67 -0.2870332531396258 1102 68-69 -0.30952624330153355 1102 70-71 -0.3410491290844675 1102 72-73 -0.37064702658771154 1102 74-75 -0.2988094211942709 1102 76-77 -0.3714665627995899 1102 78-79 -0.4319899577152313 1102 80-81 -0.4481555821347598 1102 82-83 -0.4862194387356169 1102 84-85 -0.4617712284170281 1102 86-87 -0.4721158611259071 1102 88-89 -0.4815557104942272 1102 90-91 -0.464242982746228 1102 92-93 -0.622376766071497 1102 94-95 -0.5853819912468055 1102 96-97 -0.5780101052865447 1102 98-99 -0.5312813689242759 1102 100-101 -0.5492876737986592 2203 1 0.026546041702182777 2203 2 0.06898514180655724 2203 3 -0.018681729193183116 2203 4 -0.008982678502199803 2203 5 0.029115490658227827 2203 6 -0.1350437083278635 2203 7 -0.10050324469376903 2203 8 -0.09584712136639695 2203 9 -0.12350018001612995 2203 10-11 -0.051276575302338756 2203 12-13 -0.07506711091777163 2203 14-15 -0.06728337448904398 2203 16-17 -0.08511051980533324 2203 18-19 -0.05442239895739931 2203 20-21 -0.07047914342228978 2203 22-23 -0.14319941138237624 2203 24-25 -0.09997149762395452 2203 26-27 -0.11550772174432922 2203 28-29 -0.1484338951533104 2203 30-31 -0.16870062741876524 2203 32-33 -0.16919406788258584 2203 34-35 -0.1369503860078467 2203 36-37 -0.14702868602846308 2203 38-39 -0.17036885888160214 2203 40-41 -0.2238059904884011 2203 42-43 -0.16080520167016488 2203 44-45 -0.1946159719403866 2203 46-47 -0.16415391842225802 2203 48-49 -0.14814595419818488 2203 50-51 -0.2084030302101496 2203 52-53 -0.197447607917681 2203 54-55 -0.23645541662702385 2203 56-57 -0.24787038832345587 2203 58-59 -0.2737916663383615 2203 60-61 -0.24376809247898024 2203 62-63 -0.3138870125924953 2203 64-65 -0.31286145960615386 2203 66-67 -0.28454737894256255 2203 68-69 -0.3100205939328191 2203 70-71 -0.29300654321519914 2203 72-73 -0.32542756600689415 2203 74-75 -0.2827979960247262 2203 76-77 -0.35736027029408035 2203 78-79 -0.28938915048733804 2203 80-81 -0.37110839391387174 2203 82-83 -0.39044131505088586 2203 84-85 -0.4436931157938133 2203 86-87 -0.48655821306471125 2203 88-89 -0.4592267271999262 2203 90-91 -0.4984362518975871 2203 92-93 -0.6005665871806514 2203 94-95 -0.5458921520563464 2203 96-97 -0.5441141009562074 2203 98-99 -0.48818157825184016 2203 100-101 -0.5613595074913533 1103 1 0.06315899244130208 1103 2 0.05112279705834766 1103 3 0.03377400618607851 1103 4 0.006062295683314289 1103 5 0.07073298921481097 1103 6 -0.0939733205779163 1103 7 -0.05239015247196477 1103 8 -0.06401220051272105 1103 9 -0.03126710640228225 1103 10-11 -0.009745622052228953 1103 12-13 -0.036360360754557064 1103 14-15 -0.04594387688258195 1103 16-17 -0.013358329463152074 1103 18-19 -0.009055458111035364 1103 20-21 -0.042861232506417934 1103 22-23 -0.08305783924697607 1103 24-25 -0.01439154635079376 1103 26-27 -0.04725883112865148 1103 28-29 -0.08307737358571643 1103 30-31 -0.03064732679615645 1103 32-33 -0.04441478551251521 1103 34-35 -0.03267647270685359 1103 36-37 -0.11467915591830291 1103 38-39 -0.06899414922146718 1103 40-41 -0.02576612273661283 1103 42-43 -0.014926143380407098 1103 44-45 -0.04421443009928083 1103 46-47 -0.04000920440967093 1103 48-49 -0.1231693237891136 1103 50-51 -0.12054165297880104 1103 52-53 -0.10050383467272184 1103 54-55 -0.062367203431684004 1103 56-57 -0.10240155660672912 1103 58-59 -0.11454906573245438 1103 60-61 -0.1283320562991932 1103 62-63 -0.06759589827635182 1103 64-65 -0.15306912523246297 1103 66-67 -0.07842256028061456 1103 68-69 -0.198876197968751 1103 70-71 -0.1227234833425257 1103 72-73 -0.1166374650887505 1103 74-75 -0.14208092734747169 1103 76-77 -0.10276751082857061 1103 78-79 -0.10953012488545966 1103 80-81 -0.23989940840027657 1103 82-83 -0.24823195937707254 1103 84-85 -0.32155822300830295 1103 86-87 -0.2125271663560966 1103 88-89 -0.28315530507656206 1103 90-91 -0.24968804148937096 1103 92-93 -0.3033751389673398 1103 94-95 -0.2164851288552896 1103 96-97 -0.1673229287777076 1103 98-99 -0.23891884133038843 1103 100-101 -0.2716150328086684 2204 1 0.03334548098998624 2204 2 0.060601947680602564 2204 3 0.05252102813184223 2204 4 0.03149520662787353 2204 5 0.04110215813510365 2204 6 -0.049716307594593445 2204 7 -0.0025611755523584634 2204 8 -0.013063908672222624 2204 9 0.012094083771671649 2204 10-11 0.035397004899863305 2204 12-13 0.03817081152877222 2204 14-15 0.02604223104683001 2204 16-17 0.012938537589739951 2204 18-19 0.02817782136125402 2204 20-21 0.04286723866493958 2204 22-23 -0.04785956202682229 2204 24-25 -0.012453549738239644 2204 26-27 0.05235872239519068 2204 28-29 -0.0525695782953548 2204 30-31 -0.059911161277156566 2204 32-33 -0.019486099953141434 2204 34-35 -0.007742039015099067 2204 36-37 -0.028748704824003823 2204 38-39 -0.010070930010002144 2204 40-41 0.024855690932859886 2204 42-43 0.02489208106701568 2204 44-45 0.008837347478767299 2204 46-47 0.002314048255925627 2204 48-49 0.00465318736345921 2204 50-51 0.002722097059773887 2204 52-53 0.05741131881450201 2204 54-55 -0.02318503542339556 2204 56-57 -0.017851919117426007 2204 58-59 0.014807840460797195 2204 60-61 0.011309309926048172 2204 62-63 0.02495155674944982 2204 64-65 -0.021432429026198463 2204 66-67 0.04011653400002757 2204 68-69 -0.02782616434257079 2204 70-71 -0.020029019516073276 2204 72-73 0.01377098515089159 2204 74-75 0.006463043947896097 2204 76-77 0.015964078956578476 2204 78-79 0.03623489409270064 2204 80-81 0.012370804672997338 2204 82-83 -0.09779852578554937 2204 84-85 -0.10615161559627495 2204 86-87 -0.0497883337194196 2204 88-89 -0.06122568357162805 2204 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0.5154008336179459 1214 92-93 0.4978969617126836 1214 94-95 0.4674592083269218 1214 96-97 0.48855062746173417 1214 98-99 0.5158248807949661 1214 100-101 0.5721023357430894 1215 1 -0.1254400582491897 1215 2 -0.06776723288564312 1215 3 -0.015857861730403044 1215 4 -0.0335829543056434 1215 5 -0.024346525802322105 1215 6 0.02043318076913181 1215 7 -0.016843997734682148 1215 8 -0.013969225718319933 1215 9 -0.014880751197743791 1215 10-11 -0.038713293058314946 1215 12-13 0.02100633667805596 1215 14-15 0.04053951957310886 1215 16-17 -0.0099239068871384 1215 18-19 0.04199093646674612 1215 20-21 -0.01584828547773043 1215 22-23 0.01814951109614782 1215 24-25 -0.024822709360307726 1215 26-27 0.020863488597306912 1215 28-29 0.006870702759485425 1215 30-31 0.024517071003600677 1215 32-33 0.026910473095490772 1215 34-35 -0.01598096129210802 1215 36-37 0.05239584047063772 1215 38-39 0.03959084602075791 1215 40-41 0.013906944419055378 1215 42-43 0.047141311459924395 1215 44-45 0.09432060343711868 1215 46-47 0.09198014167798618 1215 48-49 0.08794082016008531 1215 50-51 0.11482236173497284 1215 52-53 0.11859323070454764 1215 54-55 0.10115411908118688 1215 56-57 0.17415203215390562 1215 58-59 0.09597816270596837 1215 60-61 0.1357848272425528 1215 62-63 0.10446532482266235 1215 64-65 0.1966321274853584 1215 66-67 0.23195874383188197 1215 68-69 0.23914794518320548 1215 70-71 0.19198880007196806 1215 72-73 0.25157639924862707 1215 74-75 0.2174219153027579 1215 76-77 0.31461086194207866 1215 78-79 0.2226593032728772 1215 80-81 0.24026452594897307 1215 82-83 0.3424784293010319 1215 84-85 0.2694081690804424 1215 86-87 0.30359761930162676 1215 88-89 0.3491108426822862 1215 90-91 0.33671731250048964 1215 92-93 0.4215890055713629 1215 94-95 0.39180838582644384 1215 96-97 0.478387790979923 1215 98-99 0.3709277917354683 1215 100-101 0.4791092177952265 1212 1 -0.08924335189197308 1212 2 -0.05208647208498007 1212 3 -0.06995466760576363 1212 4 0.004051303669491801 1212 5 0.005433174232095439 1212 6 -0.07632843626588226 1212 7 -0.07627799363550025 1212 8 -0.1999119257493689 1212 9 -0.12364584777640175 1212 10-11 -0.213725794719835 1212 12-13 -0.20001453155410331 1212 14-15 -0.08677465103227888 1212 16-17 0.01544611636632709 1212 18-19 0.008349780861237832 1212 20-21 -0.01787286582285219 1212 22-23 0.025453075413786053 1212 24-25 0.0049527537080251705 1212 26-27 0.03345975688565517 1212 28-29 0.062114569010432774 1212 30-31 -0.004148919093957204 1212 32-33 0.0038171693650923544 1212 34-35 4.10126456138471E-4 1212 36-37 -0.011334083878331569 1212 38-39 -0.04432621818426696 1212 40-41 -0.11898109297057857 1212 42-43 -0.1484085984566974 1212 44-45 0.015346584860019163 1212 46-47 -0.004041932564369688 1212 48-49 0.037755181084222045 1212 50-51 0.030917354498058103 1212 52-53 -0.029997840807013176 1212 54-55 -0.04917907463700999 1212 56-57 -0.023464879999615107 1212 58-59 0.08503065712385904 1212 60-61 -0.20239731265087357 1212 62-63 -0.17179204720062557 1212 64-65 -0.11472433223681833 1212 66-67 -0.06966034465138193 1212 68-69 0.11207273277989316 1212 70-71 0.08298895718652943 1212 72-73 0.19723230539613468 1212 74-75 0.11371766788757753 1212 76-77 0.11800234771860119 1212 78-79 0.23200990093100415 1212 80-81 0.20682166787783274 1212 82-83 0.16495995022678045 1212 84-85 0.2588978126838626 1212 86-87 0.25376171169173745 1212 88-89 0.27926520250222353 1212 90-91 0.2251685647159647 1212 92-93 0.3640826119623739 1212 94-95 0.27658800189455945 1212 96-97 0.30021544441159165 1212 98-99 0.2843706860756896 1212 100-101 0.32748557123063904 1213 1 -0.20627998848134865 1213 2 -0.1508305729336854 1213 3 -0.1492218172659392 1213 4 -0.14819481673418977 1213 5 -0.14990243372797352 1213 6 -0.04033192865361457 1213 7 -0.008086955610956181 1213 8 -0.053278194131323175 1213 9 -0.043813441034409095 1213 10-11 -0.06237525657839171 1213 12-13 -0.1250819879591063 1213 14-15 -0.1380297805968027 1213 16-17 -0.22285652238454645 1213 18-19 -0.2664113708165132 1213 20-21 -0.1677396505645561 1213 22-23 -0.23861227084766057 1213 24-25 -0.27322810593527436 1213 26-27 -0.1526216782554357 1213 28-29 -0.16008845971467167 1213 30-31 -0.10827489521784628 1213 32-33 -0.11490944455410812 1213 34-35 -0.12272250382751082 1213 36-37 -0.06888987621010756 1213 38-39 -0.008088449971559442 1213 40-41 0.020417741374593845 1213 42-43 0.029822399832355018 1213 44-45 0.08130564191039724 1213 46-47 0.08927995028450653 1213 48-49 0.1050029813441995 1213 50-51 0.10572221726471298 1213 52-53 0.13450629088409727 1213 54-55 0.12637187515628767 1213 56-57 0.14474395358617898 1213 58-59 0.1905590436459761 1213 60-61 0.18237705626351186 1213 62-63 0.20219945779808768 1213 64-65 0.18206631120163053 1213 66-67 0.19646254355845372 1213 68-69 0.24352233417732805 1213 70-71 0.20910829312317247 1213 72-73 0.21100185696729312 1213 74-75 0.23254349698755306 1213 76-77 0.2783719864741059 1213 78-79 0.3145134377552523 1213 80-81 0.3049330177341858 1213 82-83 0.29880641429873833 1213 84-85 0.31687746073185963 1213 86-87 0.29843466579428224 1213 88-89 0.3550848740248469 1213 90-91 0.3560955837205029 1213 92-93 0.3719411239155903 1213 94-95 0.3230591292697369 1213 96-97 0.349252720411684 1213 98-99 0.43776897487234123 1213 100-101 0.19330490029904013 >>END_MODULE >>Per sequence quality scores pass #Quality Count 13 22.0 14 56.0 15 122.0 16 160.0 17 247.0 18 536.0 19 1283.0 20 2967.0 21 6389.0 22 11859.0 23 20466.0 24 31152.0 25 44054.0 26 60440.0 27 79303.0 28 101755.0 29 127276.0 30 155603.0 31 189618.0 32 229221.0 33 280311.0 34 348742.0 35 453499.0 36 640850.0 37 1033408.0 38 2338381.0 39 8950026.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 18.264127959692594 30.556850854023644 29.871579630890395 21.307441555393368 2 20.040295978656196 30.977756987805765 29.719750397661166 19.262196635876872 3 20.107910207121567 29.766597876347667 29.866791512115704 20.25870040441506 4 20.046564192964325 29.468803619017685 30.183602504304748 20.301029683713242 5 20.518951352828353 29.81345059928596 30.08499460393748 19.5826034439482 6 20.399826684933675 29.964833933533168 29.37169449367232 20.26364488786084 7 20.553410151322375 30.14778644014799 29.678411326216363 19.62039208231327 8 19.986187202590145 30.12470636718464 29.808901126039526 20.080205304185693 9 19.91591432170467 29.806946484493164 30.22448221743128 20.052656976370887 10-11 20.090657468029978 30.01553971055643 29.843432633829032 20.05037018758457 12-13 19.879221467652876 30.034496568628473 30.0525436159869 20.033738347731752 14-15 19.893072399708256 29.925725287484216 30.11037948267304 20.070822830134492 16-17 19.96538546646317 29.811755424961344 30.120459159199253 20.102399949376228 18-19 19.94084028153505 29.712959828686557 30.137318300162047 20.208881589616347 20-21 20.02814122306925 29.642659751001638 30.066532165983904 20.26266685994521 22-23 20.10059893905855 29.62793047313445 29.991138963843238 20.280331623963757 24-25 20.156277484872408 29.583079669249063 29.945483251385834 20.31515959449269 26-27 20.2305775167874 29.575967232400913 29.84180664893764 20.351648601874047 28-29 20.24290931187113 29.639321290300373 29.78044334300403 20.337326054824462 30-31 20.329417832652766 29.52087191210291 29.807937508027965 20.341772747216364 32-33 20.377716957990682 29.562841544523106 29.73023091962682 20.329210577859392 34-35 20.383322871831908 29.54266331567648 29.711509135430962 20.36250467706065 36-37 20.406959652621072 29.55050632727888 29.689457633157033 20.353076386943012 38-39 20.421145634804557 29.485284981494974 29.70301004796829 20.390559335732178 40-41 20.463574661809176 29.510331667158024 29.64266846619823 20.383425204834573 42-43 20.45646656834079 29.491073543546275 29.649529019573418 20.402930868539517 44-45 20.433049638206793 29.416900762312736 29.665047112059078 20.485002487421394 46-47 20.473906121027284 29.471443416025156 29.62529751906659 20.42935294388097 48-49 20.454452622153962 29.421346966404453 29.63454189471663 20.48965851672495 50-51 20.444405828186337 29.4443098392019 29.61599772274409 20.49528660986767 52-53 20.46119806791557 29.454019516671714 29.649251112386466 20.43553130302625 54-55 20.47454402829082 29.393126605128884 29.64566737961043 20.486661986969867 56-57 20.501071509369357 29.409816710581254 29.62199691480768 20.467114865241705 58-59 20.472578290210144 29.427284156150176 29.63713132064833 20.463006232991354 60-61 20.46278036392733 29.414230065429393 29.550322857552132 20.57266671309115 62-63 20.45780310258071 29.40279387220436 29.644330244758955 20.49507278045598 64-65 20.4940540611742 29.430835876745032 29.57574406851592 20.49936599356485 66-67 20.488721033092894 29.43701409612712 29.544875058679803 20.52938981210018 68-69 20.43479977695311 29.390353609159074 29.609034756198938 20.56581185768888 70-71 20.532429090686197 29.42193976609662 29.522548708688625 20.52308243452856 72-73 20.527515804163503 29.425535630818366 29.534501444829637 20.512447120188494 74-75 20.51159724000114 29.412441948587638 29.565482443343537 20.510478368067687 76-77 20.489489699266315 29.4458391670554 29.54097532439026 20.523695809288025 78-79 20.51015812235939 29.44099106203891 29.56130212867056 20.48754868693113 80-81 20.53160511496475 29.41765486530995 29.52705998107582 20.523680038649477 82-83 20.490859521513862 29.481625671480487 29.573186075587248 20.4543287314184 84-85 20.510271971838115 29.43947039427308 29.531544564312078 20.518713069576723 86-87 20.472531778255963 29.449926533093908 29.532617144784968 20.544924543865168 88-89 20.519621144366244 29.407663447636846 29.509460053628832 20.563255354368078 90-91 20.501129573239197 29.394313287089073 29.51013190308226 20.594425236589466 92-93 20.48631805327132 29.399956603866954 29.534868594291343 20.578856748570384 94-95 20.51539572947307 29.396317839625524 29.49606606415645 20.592220366744957 96-97 20.5368457389294 29.456026209252396 29.446599373135257 20.56052867868295 98-99 20.52115830004144 29.441698707496677 29.446643383882332 20.59049960857955 100-101 20.592200735358556 29.444462610271348 29.351271918246898 20.612064736123198 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 506.0 1 458.0 2 417.5 3 500.5 4 601.5 5 702.5 6 828.5 7 1034.5 8 1327.5 9 1636.0 10 2056.5 11 2533.5 12 3159.5 13 4125.0 14 5280.5 15 7090.5 16 10007.5 17 14784.5 18 22262.5 19 32582.0 20 46878.5 21 64976.5 22 86753.5 23 112451.5 24 141932.0 25 175693.5 26 213644.0 27 254024.5 28 295284.5 29 336920.5 30 377856.5 31 416977.5 32 453988.0 33 489616.0 34 523926.0 35 555722.5 36 582598.0 37 605357.0 38 617656.5 39 615572.0 40 604543.0 41 583917.0 42 553668.5 43 518807.0 44 486036.5 45 457977.0 46 433858.0 47 415701.0 48 398052.0 49 375604.5 50 350978.5 51 324283.0 52 296627.0 53 270724.5 54 248158.5 55 228011.0 56 209289.0 57 191232.5 58 171706.5 59 151090.0 60 130158.0 61 109255.5 62 90743.5 63 75020.0 64 61826.0 65 51111.5 66 42351.0 67 34913.0 68 28553.0 69 23113.0 70 18348.5 71 14691.0 72 11847.5 73 9686.5 74 8267.5 75 7145.0 76 6242.0 77 5540.0 78 4994.5 79 4420.5 80 3758.0 81 3228.0 82 2828.5 83 2404.0 84 2003.5 85 1635.0 86 1255.5 87 908.0 88 635.5 89 445.5 90 280.0 91 165.0 92 103.0 93 55.0 94 28.0 95 21.5 96 14.0 97 5.5 98 4.0 99 2.5 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0831030651428744 2 2.31669237753931E-4 3 0.0 4 0.0 5 0.002872698548148744 6 0.0 7 0.0 8 1.1252505833762362E-4 9 0.003547848898174486 10-11 0.0 12-13 7.611989240486304E-5 14-15 3.110986906981359E-4 16-17 0.0014495875162317397 18-19 0.0 20-21 1.0590593725893989E-4 22-23 2.1181187451787978E-4 24-25 2.31669237753931E-5 26-27 0.003345965705274632 28-29 0.0016944949961430382 30-31 0.0026311006287767875 32-33 0.0030117000908011027 34-35 0.005086794548968456 36-37 0.006718407894863998 38-39 0.008664429491997019 40-41 0.01054095031780386 42-43 0.01901011573797971 44-45 0.011523889797988397 46-47 0.01457199505472226 48-49 0.015362980023624968 50-51 0.012218897511250187 52-53 0.017269286894285883 54-55 0.013678413709099953 56-57 0.011374959573718013 58-59 0.008442688935861115 60-61 0.0051231997149012165 62-63 0.0019228546733576271 64-65 0.0016514707091315937 66-67 0.00221078644028037 68-69 0.009965086783958375 70-71 0.004891530477147286 72-73 0.0016911854356036963 74-75 0.021492286142486113 76-77 0.03429366630866047 78-79 0.037358319368091045 80-81 0.025089778448750728 82-83 0.04491735563994788 84-85 0.02035710687749185 86-87 0.01751088481365784 88-89 0.017378502392084164 90-91 0.006185568648029958 92-93 0.017960985047008337 94-95 0.021290402949586257 96-97 0.012811308847792384 98-99 0.003901971875884066 100-101 9.26676951015724E-5 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1.5107746E7 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 94.99478505677989 #Duplication Level Percentage of deduplicated Percentage of total 1 98.16085069714137 93.24768912965607 2 1.1776632415617592 2.2374373300285995 3 0.23252395676206056 0.6626568987949176 4 0.11190436072990467 0.42521322777814613 5 0.08427971220768543 0.40030665729081727 6 0.03951657531223271 0.22523211467793602 7 0.047055996872147385 0.3129052015951503 8 0.02306354241271424 0.17527330033149727 9 0.011717916157324235 0.10018268340105455 >10 0.1101629392876586 2.112337303355612 >50 0.0012173271531458652 0.06338292684971246 >100 3.455817402919045E-5 0.013952096833895391 >500 0.0 0.0 >1k 9.176227956161217E-6 0.02343112940656453 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 2.6476484314734972E-5 0.0 6.619121078683743E-6 2 2.6476484314734972E-5 0.0 1.3238242157367486E-5 3 3.309560539341871E-5 0.0 3.971472647210246E-5 4 3.971472647210246E-5 0.0 5.2952968629469945E-5 5 5.2952968629469945E-5 0.0 5.2952968629469945E-5 6 5.2952968629469945E-5 6.619121078683743E-6 7.942945294420492E-5 7 5.2952968629469945E-5 6.619121078683743E-6 1.0590593725893989E-4 8 7.281033186552116E-5 6.619121078683743E-6 1.1914417941630737E-4 9 8.604857402288866E-5 6.619121078683743E-6 1.2576330049499112E-4 10-11 1.1583461887696549E-4 6.619121078683743E-6 1.5885890588840983E-4 12-13 1.4231110319170046E-4 6.619121078683743E-6 1.9857363236051227E-4 14-15 1.8202582966380293E-4 6.619121078683743E-6 2.2505011667524723E-4 16-17 2.184309955965635E-4 6.619121078683743E-6 2.5152660098998224E-4 18-19 2.713839642260335E-4 6.619121078683743E-6 2.8131264584405904E-4 20-21 3.210273723161615E-4 6.619121078683743E-6 3.0778913015879405E-4 22-23 3.772899014849733E-4 6.619121078683743E-6 3.144082512374778E-4 24-25 4.2031418849641767E-4 1.6547802696709356E-5 3.309560539341871E-4 26-27 4.898149598225969E-4 2.6476484314734972E-5 3.6074209878826397E-4 28-29 5.824826549241694E-4 2.9786044854076842E-5 3.87218583102999E-4 30-31 6.817694711044255E-4 3.309560539341871E-5 4.1038550687839205E-4 32-33 7.645084845879723E-4 3.309560539341871E-5 4.401715517324689E-4 34-35 8.73723982386254E-4 3.640516593276059E-5 4.7988627820457137E-4 36-37 9.697012380271683E-4 3.971472647210246E-5 4.931245203619388E-4 38-39 0.0010458211304320312 3.971472647210246E-5 5.162914441373319E-4 40-41 0.001161655749308997 3.971472647210246E-5 5.262201257553575E-4 42-43 0.001287419049803988 4.964340809012807E-5 5.6593485222746E-4 44-45 0.0014429683951530559 5.2952968629469945E-5 5.857922154635112E-4 46-47 0.001595208179962782 5.9572089708153684E-5 6.056495786995624E-4 48-49 0.0018070200544806616 6.619121078683742E-5 6.354356235536393E-4 50-51 0.0019890458841444647 6.619121078683742E-5 6.453643051716649E-4 52-53 0.002181000395426293 7.281033186552116E-5 6.850790316437674E-4 54-55 0.0024523843596523267 7.281033186552116E-5 7.18174637037186E-4 56-57 0.0028164360189799325 7.281033186552116E-5 7.281033186552117E-4 58-59 0.0033029414182631873 7.281033186552116E-5 7.545798029699466E-4 60-61 0.003905281436423408 7.611989240486304E-5 7.777467267453397E-4 62-63 0.00473929069233756 8.604857402288866E-5 8.042232110600748E-4 64-65 0.005947280289197342 9.26676951015724E-5 8.306996953748097E-4 66-67 0.007191675051989886 1.0921549779828175E-4 8.50557058610861E-4 68-69 0.00912776796750488 1.1914417941630737E-4 8.637953007682285E-4 70-71 0.01191772750217008 1.2576330049499112E-4 8.968909061616471E-4 72-73 0.015849485422908222 1.2576330049499112E-4 9.00200466700989E-4 74-75 0.021472428779250062 1.2576330049499112E-4 9.134387088583565E-4 76-77 0.02884943922144309 1.2576330049499112E-4 9.332960720944077E-4 78-79 0.03881121644486213 1.3238242157367485E-4 9.564629958698008E-4 80-81 0.05208917332870171 1.3569198211301674E-4 9.76320359105852E-4 82-83 0.06892821735287316 1.390015426523586E-4 9.796299196451939E-4 84-85 0.09096658098435068 1.4231110319170046E-4 9.961777223419033E-4 86-87 0.11865436445648478 1.4562066373104233E-4 0.0010094159644992709 88-89 0.15351065605683337 1.5223978480972608E-4 0.0010392020093533477 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTCGTAT 1180 0.0 12.681624 42-43 TATGCCG 1250 0.0 11.781137 46-47 TCTCGTA 1265 0.0 10.703501 40-41 CGTATGC 1790 0.0 8.890023 44-45 ATGCCGT 1650 0.0 8.781151 46-47 AGATCGG 5950 0.0 8.223102 94-95 TAGATCG 2405 0.0 7.703089 94-95 ATCTCGT 1825 0.0 7.6794596 40-41 ATCGGAA 5360 0.0 7.6659627 94-95 GCCGTCT 2035 0.0 7.586514 48-49 TGCCGTC 1955 0.0 7.4109926 48-49 GATCGGA 5450 0.0 7.4086294 94-95 TCGGAAG 5355 0.0 7.052175 94-95 CGGAAGA 5345 0.0 6.8431873 94-95 TCGTATG 2335 0.0 6.612152 42-43 CGTCTGA 2410 0.0 6.1084957 16-17 ACACGTC 2645 0.0 5.9249454 12-13 CGTCTTC 2900 0.0 5.405471 50-51 AAGATCG 3660 0.0 5.3861995 94-95 ACGTCTG 3085 0.0 5.3108015 14-15 >>END_MODULE