FastQCFastQC Report
Fri 3 Apr 2015
H2K5YBCXX l01n01 61439-bar3.341000000043d8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2K5YBCXX l01n01 61439-bar3.341000000043d8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15107746
Sequences flagged as poor quality0
Sequence length101
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT11800.012.68162442-43
TATGCCG12500.011.78113746-47
TCTCGTA12650.010.70350140-41
CGTATGC17900.08.89002344-45
ATGCCGT16500.08.78115146-47
AGATCGG59500.08.22310294-95
TAGATCG24050.07.70308994-95
ATCTCGT18250.07.679459640-41
ATCGGAA53600.07.665962794-95
GCCGTCT20350.07.58651448-49
TGCCGTC19550.07.410992648-49
GATCGGA54500.07.408629494-95
TCGGAAG53550.07.05217594-95
CGGAAGA53450.06.843187394-95
TCGTATG23350.06.61215242-43
CGTCTGA24100.06.108495716-17
ACACGTC26450.05.924945412-13
CGTCTTC29000.05.40547150-51
AAGATCG36600.05.386199594-95
ACGTCTG30850.05.310801514-15
Produced by FastQC (version 0.11.2)