##FastQC	0.11.2
>>Basic Statistics	pass
#Measure	Value
Filename	H2K5YBCXX l01n01 60355-bar2.341000000043be.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	15805611
Sequences flagged as poor quality	0
Sequence length	101
%GC	30
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	37.114776328482336	38.0	38.0	38.0	32.0	38.0
2	37.1093645794522	38.0	38.0	38.0	32.0	38.0
3	37.09165605809228	38.0	38.0	38.0	32.0	38.0
4	37.080679323311195	38.0	38.0	38.0	32.0	38.0
5	37.06364853595347	38.0	38.0	38.0	32.0	38.0
6	38.973533386339824	40.0	38.0	40.0	38.0	40.0
7	38.92973280185119	40.0	38.0	40.0	38.0	40.0
8	38.91346996962028	40.0	38.0	40.0	38.0	40.0
9	38.92532930235978	40.0	38.0	40.0	38.0	40.0
10-11	38.92253447209349	40.0	38.0	40.0	38.0	40.0
12-13	38.90777803528127	40.0	38.0	40.0	38.0	40.0
14-15	38.89137319651863	40.0	38.0	40.0	38.0	40.0
16-17	38.87793274806017	40.0	38.0	40.0	38.0	40.0
18-19	38.83915085598399	40.0	38.0	40.0	38.0	40.0
20-21	38.817855348964365	40.0	38.0	40.0	38.0	40.0
22-23	38.80127870412602	40.0	38.0	40.0	38.0	40.0
24-25	38.76525444033767	40.0	38.0	40.0	38.0	40.0
26-27	38.701293420418864	40.0	38.0	40.0	38.0	40.0
28-29	38.651340400570405	40.0	38.0	40.0	38.0	40.0
30-31	38.632711225146565	40.0	38.0	40.0	38.0	40.0
32-33	38.592825579473015	40.0	38.0	40.0	38.0	40.0
34-35	38.567932552559974	40.0	38.0	40.0	38.0	40.0
36-37	38.53945472275637	40.0	38.0	40.0	38.0	40.0
38-39	38.50897491403528	40.0	38.0	40.0	38.0	40.0
40-41	38.47601813685026	40.0	38.0	40.0	38.0	40.0
42-43	38.419330483332786	40.0	38.0	40.0	38.0	40.0
44-45	38.39439604707468	40.0	38.0	40.0	38.0	40.0
46-47	38.35761113569099	40.0	38.0	40.0	38.0	40.0
48-49	38.28982530317873	40.0	38.0	40.0	38.0	40.0
50-51	38.2722943769779	40.0	38.0	40.0	38.0	40.0
52-53	38.24605398045036	40.0	38.0	40.0	38.0	40.0
54-55	38.17823679831169	40.0	38.0	40.0	38.0	40.0
56-57	38.16608775832836	40.0	38.0	40.0	38.0	40.0
58-59	38.13560513415141	40.0	38.0	40.0	38.0	40.0
60-61	38.0765374714081	40.0	38.0	40.0	38.0	40.0
62-63	38.04804024975687	40.0	38.0	40.0	38.0	40.0
64-65	37.983290775661885	40.0	38.0	40.0	38.0	40.0
66-67	37.928505895785996	40.0	38.0	40.0	38.0	40.0
68-69	37.88375507912981	40.0	38.0	40.0	38.0	40.0
70-71	37.86463506535749	40.0	38.0	40.0	38.0	40.0
72-73	37.833440067581066	40.0	38.0	40.0	32.0	40.0
74-75	37.75256527571126	40.0	38.0	40.0	32.0	40.0
76-77	37.690153863713334	40.0	38.0	40.0	32.0	40.0
78-79	37.613274678213955	40.0	38.0	40.0	32.0	40.0
80-81	37.57373251815447	40.0	38.0	40.0	32.0	40.0
82-83	37.50158845488478	40.0	38.0	40.0	32.0	40.0
84-85	37.46716925400733	40.0	38.0	40.0	32.0	40.0
86-87	37.42055356164339	40.0	38.0	40.0	32.0	40.0
88-89	37.351474106252525	40.0	38.0	40.0	32.0	40.0
90-91	37.29770595391725	38.0	38.0	40.0	32.0	40.0
92-93	37.21529474564444	38.0	38.0	40.0	32.0	40.0
94-95	37.139537250410626	38.0	38.0	40.0	32.0	40.0
96-97	37.05913551839281	38.0	38.0	40.0	32.0	40.0
98-99	37.03582287960902	38.0	38.0	40.0	32.0	40.0
100-101	36.2152825664253	38.0	38.0	40.0	29.5	40.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
2201	1	0.1093193192791233
2201	2	0.07575019959602969
2201	3	0.05246268705015211
2201	4	0.05897206908494468
2201	5	0.01497307757350086
2201	6	0.054468390080451456
2201	7	0.01808680875591051
2201	8	-0.014705362778869357
2201	9	-0.016787950352579628
2201	10-11	-0.03292895038637056
2201	12-13	-0.018679739010359242
2201	14-15	-0.02570695889770036
2201	16-17	-0.280661826895809
2201	18-19	-0.23474297586929538
2201	20-21	-0.2104992527470131
2201	22-23	-0.2456575886741419
2201	24-25	-0.2561472272870233
2201	26-27	-0.24724856969413622
2201	28-29	-0.25427428304970334
2201	30-31	-0.255040048077376
2201	32-33	-0.2799718259940107
2201	34-35	-0.2670587978095753
2201	36-37	-0.28669391984205816
2201	38-39	-0.30042460302654206
2201	40-41	-0.3261743683011389
2201	42-43	-0.27402305666695526
2201	44-45	-0.35920085998894535
2201	46-47	-0.3226935580423813
2201	48-49	-0.3167535489728124
2201	50-51	-0.4188065079063321
2201	52-53	-0.30497986605320904
2201	54-55	-0.3184484273796784
2201	56-57	-0.4163225173976244
2201	58-59	-0.37056097839320046
2201	60-61	-0.3849343806739398
2201	62-63	-0.36026111678798145
2201	64-65	-0.45495103792445946
2201	66-67	-0.3655951359291336
2201	68-69	-0.40906628315853766
2201	70-71	-0.5254603849451556
2201	72-73	-0.4178665250332614
2201	74-75	-0.3449169527443132
2201	76-77	-0.47626070198896286
2201	78-79	-0.4510487772964282
2201	80-81	-0.4531711404079104
2201	82-83	-0.5358240434855261
2201	84-85	-0.49022468387945395
2201	86-87	-0.5820384556919436
2201	88-89	-0.6340471724807983
2201	90-91	-0.6010937464348913
2201	92-93	-0.6442701206711021
2201	94-95	-0.6262055347411106
2201	96-97	-0.6303175614735039
2201	98-99	-0.5613147536707004
2201	100-101	-0.6565547382835106
1101	1	0.08671690750686167
1101	2	0.09146509475881004
1101	3	0.06600957051830392
1101	4	0.021979749591665154
1101	5	0.05546903768460254
1101	6	0.044811655314916266
1101	7	0.07121054205428123
1101	8	0.05775032891926202
1101	9	-0.037649144929353895
1101	10-11	-0.006978915881454384
1101	12-13	0.019434915975871547
1101	14-15	0.0015731851787919027
1101	16-17	-0.04578476736656967
1101	18-19	-0.13286946282870815
1101	20-21	-0.1081553839701499
1101	22-23	-0.17781064472016794
1101	24-25	-0.11782182176708744
1101	26-27	-0.0679748903209969
1101	28-29	-0.1467930230088399
1101	30-31	-0.1705690574362393
1101	32-33	-0.1498427298381273
1101	34-35	-0.13015938578426756
1101	36-37	-0.14983926749007992
1101	38-39	-0.1411917356192447
1101	40-41	-0.12368180586722843
1101	42-43	-0.12398072106214642
1101	44-45	-0.1308739584098717
1101	46-47	-0.1392508035351554
1101	48-49	-0.1479752151680387
1101	50-51	-0.21494671823305822
1101	52-53	-0.18930917242202128
1101	54-55	-0.2748540402707036
1101	56-57	-0.2218034714287569
1101	58-59	-0.2470214272104414
1101	60-61	-0.25386418033758673
1101	62-63	-0.26773707053256146
1101	64-65	-0.27124758630613144
1101	66-67	-0.295998035032504
1101	68-69	-0.30872711488314053
1101	70-71	-0.3053496876737043
1101	72-73	-0.32699588513173694
1101	74-75	-0.2818959510889556
1101	76-77	-0.33739302891891754
1101	78-79	-0.34615379897947207
1101	80-81	-0.31394760852513315
1101	82-83	-0.3465500291144892
1101	84-85	-0.37893192505006823
1101	86-87	-0.4444558944127621
1101	88-89	-0.4314022359945682
1101	90-91	-0.47271200748515696
1101	92-93	-0.510895702191668
1101	94-95	-0.4304264211380584
1101	96-97	-0.43801623408941737
1101	98-99	-0.4602458020777789
1101	100-101	-0.5617445294208352
2202	1	0.047888723604302186
2202	2	0.038428439432536265
2202	3	0.03468507831994572
2202	4	0.05207473607998736
2202	5	0.015456868052119432
2202	6	-0.05163156898049692
2202	7	-0.052009908204020405
2202	8	-0.05214774377204634
2202	9	-0.03867373302104937
2202	10-11	0.0128717322951033
2202	12-13	-0.03701195117807288
2202	14-15	-0.03800943453336458
2202	16-17	-0.0590415164801783
2202	18-19	-0.01750399717090545
2202	20-21	-0.055459926874455334
2202	22-23	-0.1139643550533549
2202	24-25	-0.050349119268950915
2202	26-27	-0.11291381652238641
2202	28-29	-0.10058245245751607
2202	30-31	-0.09949515934408026
2202	32-33	-0.11470495535607483
2202	34-35	-0.15308864867396466
2202	36-37	-0.0886533123500044
2202	38-39	-0.14605296979544846
2202	40-41	-0.17559145524676012
2202	42-43	-0.14753277431557166
2202	44-45	-0.1516606224661956
2202	46-47	-0.13413258015128804
2202	48-49	-0.19904060690029723
2202	50-51	-0.21027659908640572
2202	52-53	-0.23082595549524143
2202	54-55	-0.17626575884457907
2202	56-57	-0.20986901221092324
2202	58-59	-0.19692995807202607
2202	60-61	-0.14441356921883397
2202	62-63	-0.2194725983223762
2202	64-65	-0.2274529129868199
2202	66-67	-0.2308655476014181
2202	68-69	-0.2597324044480658
2202	70-71	-0.31858025765322395
2202	72-73	-0.30108286987373845
2202	74-75	-0.32188725722220113
2202	76-77	-0.31050848530797026
2202	78-79	-0.2976520921492849
2202	80-81	-0.2890105719678715
2202	82-83	-0.3721909493949127
2202	84-85	-0.41401472230043623
2202	86-87	-0.3437718205128206
2202	88-89	-0.4470438412169102
2202	90-91	-0.4338162629348048
2202	92-93	-0.3824712223158855
2202	94-95	-0.38055863500611053
2202	96-97	-0.4019883742990231
2202	98-99	-0.49569990933851216
2202	100-101	-0.5469519031207994
1102	1	0.05746494853203643
1102	2	0.053747370332288824
1102	3	0.050082090300740845
1102	4	0.03957040442014659
1102	5	0.013140383216317275
1102	6	-0.11260945125000177
1102	7	-0.11349199461946569
1102	8	-0.09761245885473357
1102	9	-0.07655192717006543
1102	10-11	-0.056174607780647534
1102	12-13	-0.05130249678504839
1102	14-15	-0.040340786282357044
1102	16-17	-0.054331299757386375
1102	18-19	-0.0660019994178569
1102	20-21	-0.09313895998867139
1102	22-23	-0.1935706516557616
1102	24-25	-0.1475681804150355
1102	26-27	-0.13342046609682257
1102	28-29	-0.2121185231103766
1102	30-31	-0.18987672456235316
1102	32-33	-0.2014126322858445
1102	34-35	-0.20311606310303176
1102	36-37	-0.21508493122155414
1102	38-39	-0.2526952577252217
1102	40-41	-0.22856028116995475
1102	42-43	-0.27160492271718084
1102	44-45	-0.2476009040219651
1102	46-47	-0.2915572913492568
1102	48-49	-0.30471947119628595
1102	50-51	-0.29921415413237895
1102	52-53	-0.2640773491459001
1102	54-55	-0.3834572170443806
1102	56-57	-0.3446853318293748
1102	58-59	-0.33082082227311815
1102	60-61	-0.3163405372221604
1102	62-63	-0.4111439655636744
1102	64-65	-0.3912045349763247
1102	66-67	-0.39327620844114364
1102	68-69	-0.4155219670118484
1102	70-71	-0.45470309166444167
1102	72-73	-0.39430541880031456
1102	74-75	-0.4025053379516308
1102	76-77	-0.3755101777266532
1102	78-79	-0.48865287290713866
1102	80-81	-0.49182893360914903
1102	82-83	-0.5627684004259734
1102	84-85	-0.5260200075608878
1102	86-87	-0.5031481880570183
1102	88-89	-0.543560768369197
1102	90-91	-0.5473362655655691
1102	92-93	-0.7540997294246736
1102	94-95	-0.6655277859626594
1102	96-97	-0.6583535975097732
1102	98-99	-0.6638000041478449
1102	100-101	-0.7211928671089325
2203	1	0.0701425152034858
2203	2	0.020718771739474562
2203	3	0.007070084574834823
2203	4	0.011584282080569608
2203	5	0.009933583612799168
2203	6	-0.15860370697851778
2203	7	-0.17577209681336114
2203	8	-0.11838356230308023
2203	9	-0.1802050368788528
2203	10-11	-0.10101530355258603
2203	12-13	-0.11854352821972469
2203	14-15	-0.10764981432252796
2203	16-17	-0.1125179878835425
2203	18-19	-0.08351391858751356
2203	20-21	-0.12323457522472125
2203	22-23	-0.22325740205140931
2203	24-25	-0.12544362746657356
2203	26-27	-0.13761361650661286
2203	28-29	-0.19610062022360353
2203	30-31	-0.22490997540940327
2203	32-33	-0.2336553072754768
2203	34-35	-0.22584864761503098
2203	36-37	-0.23569551507380737
2203	38-39	-0.23526857690283975
2203	40-41	-0.27283613637222714
2203	42-43	-0.24463238170938695
2203	44-45	-0.2593043484239246
2203	46-47	-0.2108806408021593
2203	48-49	-0.2778326497559078
2203	50-51	-0.27652389917077613
2203	52-53	-0.23513901688654215
2203	54-55	-0.2839432491997371
2203	56-57	-0.32535249441816205
2203	58-59	-0.373800269396078
2203	60-61	-0.3073560421275374
2203	62-63	-0.3669564083182806
2203	64-65	-0.4062643849801191
2203	66-67	-0.44887464209845973
2203	68-69	-0.4649271893998801
2203	70-71	-0.3867231328242511
2203	72-73	-0.4490229512450057
2203	74-75	-0.3824946702712282
2203	76-77	-0.49381838574392134
2203	78-79	-0.4421547598763098
2203	80-81	-0.5214459635221758
2203	82-83	-0.5183113034561231
2203	84-85	-0.6026367670638351
2203	86-87	-0.6290014220814015
2203	88-89	-0.6148378357947379
2203	90-91	-0.5909041865145426
2203	92-93	-0.7659048360394607
2203	94-95	-0.6613296649249563
2203	96-97	-0.7175004089537609
2203	98-99	-0.6973531505291675
2203	100-101	-0.7513921050843493
1103	1	0.07192282323190824
1103	2	0.061444866842016665
1103	3	0.054148478906022035
1103	4	0.07522000179012878
1103	5	0.05950373172987611
1103	6	-0.04134034980017276
1103	7	-0.005691289384529341
1103	8	-0.023542845902262854
1103	9	-0.025151291974907508
1103	10-11	0.023288274960968636
1103	12-13	-0.03726240363805999
1103	14-15	-0.04785675901719344
1103	16-17	0.001270989603156636
1103	18-19	0.012570663337641008
1103	20-21	-0.002743899101204761
1103	22-23	-0.09233939957498905
1103	24-25	-0.04390636686596139
1103	26-27	-0.05085155786103002
1103	28-29	-0.05598783197717694
1103	30-31	-0.07589998465500969
1103	32-33	-0.0747337629519933
1103	34-35	-0.07558589762601997
1103	36-37	-0.11239130874090364
1103	38-39	-0.06936071819235679
1103	40-41	0.006140828447236402
1103	42-43	-0.05995965390682301
1103	44-45	-0.060307515821676816
1103	46-47	-0.04738054350751497
1103	48-49	-0.09164671459785012
1103	50-51	-0.1203110867947359
1103	52-53	-0.09023415002586432
1103	54-55	-0.09169825760402972
1103	56-57	-0.10682138040115063
1103	58-59	-0.1205466182599153
1103	60-61	-0.10924692096068611
1103	62-63	-0.0838677436496198
1103	64-65	-0.1797519418879503
1103	66-67	-0.07198191870695325
1103	68-69	-0.2344082305400974
1103	70-71	-0.1768556926255016
1103	72-73	-0.15027074645593075
1103	74-75	-0.13633230767238302
1103	76-77	-0.14339231716307665
1103	78-79	-0.16505029979663988
1103	80-81	-0.26493708676526495
1103	82-83	-0.23335998947015213
1103	84-85	-0.3400104814843985
1103	86-87	-0.26232415048085045
1103	88-89	-0.306583712937325
1103	90-91	-0.3245175324292262
1103	92-93	-0.33176832095134756
1103	94-95	-0.2707181026545058
1103	96-97	-0.26253137756215494
1103	98-99	-0.3132393187994609
1103	100-101	-0.2714552941724193
2204	1	0.030101387439728455
2204	2	0.07945805224847646
2204	3	0.09984434250090857
2204	4	0.039852274265982146
2204	5	0.06510000291985563
2204	6	-0.02711966127831289
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1212	98-99	0.44946598775152324
1212	100-101	0.503364056481729
1213	1	-0.21586975607250025
1213	2	-0.15760012884614838
1213	3	-0.16052765349651565
1213	4	-0.13543474495513408
1213	5	-0.13205857631357532
1213	6	-0.03419405364520145
1213	7	0.023834906459249794
1213	8	-0.013064506962258804
1213	9	-0.04320979854612261
1213	10-11	-0.0390478604199771
1213	12-13	-0.0820202135990371
1213	14-15	-0.09830049111136674
1213	16-17	-0.16460299028487668
1213	18-19	-0.23077890546487367
1213	20-21	-0.12865516054285564
1213	22-23	-0.1408283032892257
1213	24-25	-0.18145233353809687
1213	26-27	-0.038194405379393004
1213	28-29	-0.07167830390052643
1213	30-31	-0.06779751726385541
1213	32-33	-0.03627303049222519
1213	34-35	-0.050894207184974505
1213	36-37	0.013460338087334378
1213	38-39	0.050082118664334985
1213	40-41	0.06463697881169139
1213	42-43	0.11058489887889067
1213	44-45	0.13697049915211323
1213	46-47	0.1377361989484882
1213	48-49	0.16228028667383398
1213	50-51	0.1950878862832539
1213	52-53	0.20710583842065233
1213	54-55	0.1996211493840292
1213	56-57	0.20009113500181996
1213	58-59	0.23673786803468744
1213	60-61	0.24143187385870846
1213	62-63	0.2692053105308361
1213	64-65	0.28095685281996907
1213	66-67	0.3046795628996577
1213	68-69	0.3575593489715416
1213	70-71	0.2567236459391964
1213	72-73	0.2629102993197989
1213	74-75	0.3397526511372604
1213	76-77	0.37057233439031734
1213	78-79	0.3711185253585043
1213	80-81	0.36328954766484145
1213	82-83	0.38039304288959386
1213	84-85	0.3923648155346484
1213	86-87	0.40561649387291254
1213	88-89	0.45362117053745266
1213	90-91	0.4733785301264959
1213	92-93	0.49313530596100463
1213	94-95	0.44362342758991247
1213	96-97	0.4638514013499133
1213	98-99	0.5085222653364241
1213	100-101	0.36189123905469245
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	19.0
14	58.0
15	153.0
16	258.0
17	463.0
18	1014.0
19	2268.0
20	4118.0
21	7481.0
22	12541.0
23	20370.0
24	31549.0
25	44921.0
26	62296.0
27	82574.0
28	106053.0
29	132962.0
30	163532.0
31	198570.0
32	240485.0
33	294709.0
34	369625.0
35	483108.0
36	678010.0
37	1101393.0
38	2493666.0
39	9273415.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	13.465731329734638	35.306385660328424	36.10518209283665	15.122700917100293
2	14.153506079400142	36.335197631673196	35.26817633939488	14.243119949531776
3	14.249496587003186	35.102097603186614	35.41647330179137	15.231932508018831
4	14.697331219906651	34.729179403440966	35.60093311166522	14.97255626498716
5	15.134094659452895	34.924060468679144	35.31490165130447	14.626943220563488
6	14.923194047987135	35.32996604813316	34.48767655992546	15.259163343954246
7	14.977263454098674	35.067590870103025	35.201328186553496	14.753817489244799
8	14.746470742759904	35.32282751572988	34.99677202853269	14.933929712977534
9	14.682379044549984	34.753844882586144	35.364163088798165	15.19961298406571
10-11	14.706755088430304	35.11353341544341	35.18768113425036	14.99203036187592
12-13	14.52072917980811	35.050460615399835	35.36322329929517	15.065586905496877
14-15	14.583935639632761	34.8865246232411	35.32824244725695	15.201297289869192
16-17	14.67788369250011	34.81684525355863	35.414322264231835	15.090948789709428
18-19	14.723116366713063	34.71277383708861	35.24509112618298	15.31901867001535
20-21	14.839540947221838	34.53065655885038	35.17568784208625	15.454114651841532
22-23	14.794686433525376	34.642566106400395	35.17233154367002	15.390415916404207
24-25	14.929836741739946	34.536964632443755	35.04824223235035	15.484956393465948
26-27	14.988787763711532	34.55612679336622	34.99349005704125	15.461595385880997
28-29	15.020876304648434	34.523396952786804	34.95744592206207	15.498280820502691
30-31	15.030542755460363	34.45844572499176	34.923842570755575	15.587168948792302
32-33	15.121496382004814	34.54214199133169	34.84492914403535	15.491432482628145
34-35	15.11135362455003	34.54651566957146	34.847265094518605	15.494865611359904
36-37	15.091215555805906	34.404991822459436	34.81785029436267	15.685942327371988
38-39	15.211436333151557	34.46167629163374	34.795895533850555	15.53099184136415
40-41	15.202522753413977	34.556259863799056	34.714596340850875	15.526621041936087
42-43	15.113735012783119	34.34357365624574	34.80720084393304	15.735490487038097
44-45	15.210378962951602	34.40339781270521	34.726927310078445	15.659295914264746
46-47	15.266725865186709	34.50580298839681	34.69597185351461	15.531499292901879
48-49	15.174541597913619	34.34259693036969	34.73665856526812	15.746202906448573
50-51	15.279800330731966	34.31094195840046	34.68600838257592	15.723249328291654
52-53	15.259671761761377	34.49270615441002	34.68153211256254	15.566089971266056
54-55	15.233815752152724	34.3646993890128	34.671431638104735	15.730053220729745
56-57	15.319655087940731	34.34239096544454	34.62024723145863	15.717706715156105
58-59	15.303126431373512	34.375379050009954	34.7072821197606	15.614212398855933
60-61	15.337878462172883	34.37696666510069	34.55657312407455	15.728581748651877
62-63	15.302460121205558	34.278280160289285	34.6418354579004	15.777424260604759
64-65	15.282370985708502	34.35062335253486	34.667677548514924	15.699328113241712
66-67	15.340904830969974	34.38129407802144	34.57531147732972	15.70248961367887
68-69	15.348783852116732	34.32142735451077	34.605311567590405	15.724477225782103
70-71	15.367822780229723	34.38937290307052	34.54270285072474	15.700101465975017
72-73	15.338240870226908	34.31973884284365	34.578388620206994	15.76363166672245
74-75	15.38118742113816	34.35531064390497	34.55638757256049	15.707114362396386
76-77	15.352637494466412	34.415495294823934	34.58053539472964	15.651331815980019
78-79	15.38522640281197	34.296243482036424	34.58818432486129	15.730345790290325
80-81	15.437628078524146	34.32690979015998	34.561762805384305	15.673699325931564
82-83	15.41276476960807	34.46610907873295	34.50622072291475	15.61490542874423
84-85	15.31764285766041	34.26525294835169	34.590758404139535	15.82634578984836
86-87	15.405310049679974	34.395407814661745	34.51653789081606	15.68274424484222
88-89	15.417787418957085	34.44655479272033	34.534108811179884	15.601548977142713
90-91	15.35770685146087	34.322326224816756	34.518739559461515	15.801227364260859
92-93	15.425412557836438	34.31983437823559	34.54538683573762	15.709366228190353
94-95	15.405532611335278	34.42685320172729	34.52629180882107	15.641322378116357
96-97	15.384290239388026	34.34643548373727	34.540891312726494	15.728382964148208
98-99	15.443516055109288	34.31654768661953	34.51345650634915	15.726479751922032
100-101	15.448445745151092	34.39183393046887	34.55391576015496	15.605804564225084
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1934.0
1	1758.5
2	1700.0
3	2012.0
4	2578.5
5	3363.5
6	4406.5
7	5657.5
8	7463.5
9	10520.0
10	15049.5
11	21506.5
12	31685.0
13	47675.0
14	71087.0
15	103645.0
16	147362.0
17	202271.0
18	266155.5
19	337896.0
20	413693.5
21	487257.0
22	558386.0
23	618948.0
24	664696.0
25	699710.5
26	718161.0
27	719942.5
28	718979.5
29	715608.0
30	706319.0
31	698074.5
32	678688.0
33	641349.5
34	586914.5
35	518980.0
36	454747.5
37	401796.0
38	356145.5
39	320627.5
40	294981.5
41	272051.0
42	252603.0
43	235126.5
44	216393.0
45	196148.0
46	179522.5
47	177916.0
48	169015.0
49	141189.5
50	112673.5
51	89360.5
52	73267.5
53	62092.5
54	54375.5
55	48276.5
56	42920.5
57	37660.5
58	33146.5
59	29299.0
60	24819.0
61	20460.0
62	16677.0
63	13355.0
64	10689.0
65	8520.5
66	6794.0
67	5267.0
68	3933.0
69	3004.0
70	2298.0
71	1768.5
72	1410.0
73	1064.0
74	819.0
75	654.0
76	506.5
77	402.5
78	361.5
79	287.5
80	216.0
81	170.0
82	112.5
83	83.0
84	56.0
85	33.5
86	18.0
87	7.0
88	5.5
89	5.5
90	2.5
91	1.5
92	1.5
93	0.5
94	1.0
95	1.5
96	0.5
97	0.0
98	0.0
99	0.5
100	1.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0836854709381371
2	1.391910758780537E-4
3	0.0
4	0.0
5	0.002973627530122056
6	0.0
7	0.0
8	1.2021047462195546E-4
9	0.0036506023082562263
10-11	0.0
12-13	1.0755674045122331E-4
14-15	2.8787245238415645E-4
16-17	0.0014741600308902957
18-19	0.0
20-21	1.3286420879268763E-4
22-23	1.9613287964634837E-4
24-25	6.010523731097773E-5
26-27	0.003524064966548905
28-29	0.0015912070719695684
30-31	0.00246747816329277
32-33	0.002840763321329368
34-35	0.004805255551335536
36-37	0.006595758936494135
38-39	0.008316666783713709
40-41	0.010179929140354017
42-43	0.018572518329092118
44-45	0.011369380152402839
46-47	0.014475871891317584
48-49	0.015010492160031016
50-51	0.011875529519232125
52-53	0.01707621426340304
54-55	0.013213661907787052
56-57	0.011113142035445514
58-59	0.008212273476805167
60-61	0.00492546602595749
62-63	0.001977145964176899
64-65	0.0017019272459634746
66-67	0.00207204897045739
68-69	0.010328610516860121
70-71	0.005096291437262375
72-73	0.001657639176365912
74-75	0.021827691444512964
76-77	0.034870527940995134
78-79	0.037999163714708656
80-81	0.025468803452141142
82-83	0.04559140421714795
84-85	0.020654057600177557
86-87	0.01749062405749452
88-89	0.017686756937140866
90-91	0.005887149822933134
92-93	0.01807585926289088
94-95	0.02150185778961661
96-97	0.012792925246610208
98-99	0.0036695829095123246
100-101	9.173957273780812E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	1.5805611E7
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.27745598728223
#Duplication Level	Percentage of deduplicated	Percentage of total
1	59.57834407314968	31.146042600792395
2	22.7267162630604	23.76189818355183
3	9.357385441992644	14.675409167994177
4	3.6980992722546167	7.733088877675646
5	1.7879669905792472	4.673518282836002
6	0.8408123567406611	2.6373318583843774
7	0.4748917374890689	1.7378292333716145
8	0.3588220108061902	1.5006641501750972
9	0.2419338593942358	1.138291801768394
>10	0.8734161145533228	8.740917505649005
>50	0.05751406737650313	1.967729462472481
>100	0.004069953517778842	0.2709089154416972
>500	0.0	0.0
>1k	2.785908567471327E-5	0.016369959887312605
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA Adapter	Nextera Transposase Sequence
1	4.4288069597562536E-5	0.0	2.5307468341464308E-5
2	4.4288069597562536E-5	0.0	2.5307468341464308E-5
3	4.4288069597562536E-5	0.0	3.1634335426830384E-5
4	4.4288069597562536E-5	0.0	5.0614936682928615E-5
5	5.0614936682928615E-5	0.0	6.959553793902685E-5
6	5.0614936682928615E-5	0.0	7.592240502439293E-5
7	5.0614936682928615E-5	0.0	9.490300628049115E-5
8	5.0614936682928615E-5	1.2653734170732154E-5	1.1388360753658938E-4
9	5.0614936682928615E-5	1.2653734170732154E-5	1.391910758780537E-4
10-11	5.0614936682928615E-5	1.2653734170732154E-5	1.4551794296341976E-4
12-13	6.643210439634381E-5	1.2653734170732154E-5	1.7082541130488408E-4
14-15	9.490300628049115E-5	1.898060125609823E-5	2.0878661381708053E-4
16-17	1.1704704107927242E-4	2.5307468341464308E-5	2.404209492439109E-4
18-19	1.5500824359146887E-4	3.4797768969513423E-5	2.4358438278659396E-4
20-21	1.8664257901829926E-4	4.4288069597562536E-5	2.562381169573261E-4
22-23	2.4991124987196005E-4	4.4288069597562536E-5	2.688918511280583E-4
24-25	3.1317992072562076E-4	4.4288069597562536E-5	3.036896200975717E-4
26-27	3.954291928353798E-4	4.4288069597562536E-5	3.1634335426830384E-4
28-29	4.903321991158709E-4	4.4288069597562536E-5	3.195067878109869E-4
30-31	5.75744904768313E-4	4.4288069597562536E-5	3.3532395552440206E-4
32-33	6.927919458475855E-4	4.4288069597562536E-5	3.479776896951342E-4
34-35	8.003486862988087E-4	5.694180376829469E-5	3.5746799032318336E-4
36-37	8.857613919512507E-4	6.959553793902685E-5	3.922657592926968E-4
38-39	9.96481565945157E-4	6.959553793902685E-5	4.1757322763416105E-4
40-41	0.0011230189076524787	6.959553793902685E-5	4.397172624329423E-4
42-43	0.0012685368506158983	7.275897148170988E-5	4.4920756306099147E-4
44-45	0.0014646697302622468	9.490300628049115E-5	4.681881643170897E-4
46-47	0.0016291682744817646	9.490300628049115E-5	4.808418984878218E-4
48-49	0.0018569354895549436	1.1072017399390634E-4	4.903321991158709E-4
50-51	0.0020277609008598277	1.2021047462195546E-4	4.998224997439201E-4
52-53	0.002233384081134225	1.2653734170732153E-4	5.124762339146522E-4
54-55	0.002476968463920819	1.2653734170732153E-4	5.251299680853844E-4
56-57	0.0027110625460793638	1.2653734170732153E-4	5.441105693414826E-4
58-59	0.003036896200975717	1.328642087926876E-4	5.78908338310996E-4
60-61	0.003308951485646458	1.328642087926876E-4	5.915620724817282E-4
62-63	0.0035841702038598823	1.328642087926876E-4	5.947255060244112E-4
64-65	0.003954291928353798	1.328642087926876E-4	5.978889395670942E-4
66-67	0.0043465576876464945	1.4235450942073674E-4	6.073792401951434E-4
68-69	0.00475780404819529	1.486813765061028E-4	6.168695408231926E-4
70-71	0.0051975213106282316	1.5184481004878585E-4	6.548307433353889E-4
72-73	0.005691016943286786	1.5184481004878585E-4	6.864650787622192E-4
74-75	0.00617502227531729	1.7082541130488408E-4	6.991188129329515E-4
76-77	0.006624229838378282	1.7715227839025014E-4	7.054456800183176E-4
78-79	0.007212628477317327	1.834791454756162E-4	7.275897148170988E-4
80-81	0.007968689094018573	1.834791454756162E-4	7.40243448987831E-4
82-83	0.00883863331825641	1.834791454756162E-4	7.528971831585632E-4
84-85	0.009850932051914982	1.834791454756162E-4	7.718777844146613E-4
86-87	0.011043546497506487	1.898060125609823E-4	8.31983021725639E-4
88-89	0.01265057073718947	1.898060125609823E-4	8.636173571524694E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGATCGG	695	4.0030813E-5	5.8097506	94-95
>>END_MODULE