##FastQC	0.11.2
>>Basic Statistics	pass
#Measure	Value
Filename	H2K5YBCXX l01n01 50617-bar10.341000000044bd.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	14184117
Sequences flagged as poor quality	0
Sequence length	101
%GC	40
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.9800600206555	38.0	38.0	38.0	32.0	38.0
2	36.96070703590502	38.0	38.0	38.0	32.0	38.0
3	36.9399739158948	38.0	38.0	38.0	32.0	38.0
4	36.93664751919348	38.0	38.0	38.0	32.0	38.0
5	36.934077320428194	38.0	38.0	38.0	32.0	38.0
6	38.8749828417236	40.0	38.0	40.0	38.0	40.0
7	38.8336799534296	40.0	38.0	40.0	38.0	40.0
8	38.827672882280936	40.0	38.0	40.0	38.0	40.0
9	38.842499113621244	40.0	38.0	40.0	38.0	40.0
10-11	38.839818051416245	40.0	38.0	40.0	38.0	40.0
12-13	38.83139338176638	40.0	38.0	40.0	38.0	40.0
14-15	38.82278138991663	40.0	38.0	40.0	38.0	40.0
16-17	38.81446994550313	40.0	38.0	40.0	38.0	40.0
18-19	38.781266856442315	40.0	38.0	40.0	38.0	40.0
20-21	38.76652198370896	40.0	38.0	40.0	38.0	40.0
22-23	38.749465581819436	40.0	38.0	40.0	38.0	40.0
24-25	38.718928925924686	40.0	38.0	40.0	38.0	40.0
26-27	38.653379656978295	40.0	38.0	40.0	38.0	40.0
28-29	38.606810772922984	40.0	38.0	40.0	38.0	40.0
30-31	38.58944420015712	40.0	38.0	40.0	38.0	40.0
32-33	38.55275961133147	40.0	38.0	40.0	38.0	40.0
34-35	38.530007331439805	40.0	38.0	40.0	38.0	40.0
36-37	38.50006838635073	40.0	38.0	40.0	38.0	40.0
38-39	38.47283732924651	40.0	38.0	40.0	38.0	40.0
40-41	38.40543782175514	40.0	38.0	40.0	38.0	40.0
42-43	38.3523655367479	40.0	38.0	40.0	38.0	40.0
44-45	38.3586639901518	40.0	38.0	40.0	38.0	40.0
46-47	38.32998585671565	40.0	38.0	40.0	38.0	40.0
48-49	38.267548307730394	40.0	38.0	40.0	38.0	40.0
50-51	38.24954549514785	40.0	38.0	40.0	38.0	40.0
52-53	38.22003385899876	40.0	38.0	40.0	38.0	40.0
54-55	38.15232167783162	40.0	38.0	40.0	38.0	40.0
56-57	38.14479572468275	40.0	38.0	40.0	38.0	40.0
58-59	38.11541345858893	40.0	38.0	40.0	38.0	40.0
60-61	38.057472382665765	40.0	38.0	40.0	38.0	40.0
62-63	38.03138831271626	40.0	38.0	40.0	38.0	40.0
64-65	37.97248464603049	40.0	38.0	40.0	38.0	40.0
66-67	37.9236071233761	40.0	38.0	40.0	38.0	40.0
68-69	37.881671590836426	40.0	38.0	40.0	38.0	40.0
70-71	37.85787797012672	40.0	38.0	40.0	35.0	40.0
72-73	37.820651225592684	40.0	38.0	40.0	32.0	40.0
74-75	37.69428019382525	40.0	38.0	40.0	32.0	40.0
76-77	37.62553051416595	40.0	38.0	40.0	32.0	40.0
78-79	37.54977116305513	40.0	38.0	40.0	32.0	40.0
80-81	37.51720723961879	40.0	38.0	40.0	32.0	40.0
82-83	37.44557429976078	40.0	38.0	40.0	32.0	40.0
84-85	37.40834769622953	40.0	38.0	40.0	32.0	40.0
86-87	37.36868065174589	40.0	38.0	40.0	32.0	40.0
88-89	37.30321270615577	39.0	38.0	40.0	32.0	40.0
90-91	37.248647236905896	38.0	38.0	40.0	32.0	40.0
92-93	37.167666376412434	38.0	38.0	40.0	32.0	40.0
94-95	37.097003923473	38.0	38.0	40.0	32.0	40.0
96-97	37.01489377167433	38.0	38.0	40.0	32.0	40.0
98-99	36.99447332533988	38.0	38.0	40.0	32.0	40.0
100-101	36.17569817705254	38.0	38.0	40.0	29.5	40.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
2201	1	0.11042926161655942
2201	2	0.12435875194428547
2201	3	0.08576085475910844
2201	4	0.10214863487864534
2201	5	0.006349035040457807
2201	6	0.04295054779053231
2201	7	0.05153254481411551
2201	8	0.012956267211393424
2201	9	-0.02820804340674954
2201	10-11	0.0018111590884899442
2201	12-13	-0.014124862196304377
2201	14-15	-0.0105062728726395
2201	16-17	-0.13929500692836427
2201	18-19	-0.09238469910702207
2201	20-21	-0.06548855511184115
2201	22-23	-0.11218663450239319
2201	24-25	-0.09813394993628322
2201	26-27	-0.1105226168561515
2201	28-29	-0.10185702346483083
2201	30-31	-0.11416057600738583
2201	32-33	-0.14968065108063655
2201	34-35	-0.1785725094116728
2201	36-37	-0.19025482690387463
2201	38-39	-0.1673204926961276
2201	40-41	-0.15832411921886091
2201	42-43	-0.1554725435580906
2201	44-45	-0.25294013333096643
2201	46-47	-0.17839140573110512
2201	48-49	-0.1705034600101527
2201	50-51	-0.27206831798541486
2201	52-53	-0.20219480099973453
2201	54-55	-0.16171020191676888
2201	56-57	-0.21522663897415129
2201	58-59	-0.2165857903081374
2201	60-61	-0.1766890913752448
2201	62-63	-0.17472219992327354
2201	64-65	-0.2508227004237895
2201	66-67	-0.22749159803893093
2201	68-69	-0.24915412717160734
2201	70-71	-0.24975198661253728
2201	72-73	-0.2340714629284406
2201	74-75	-0.22981067368633745
2201	76-77	-0.2827515443698658
2201	78-79	-0.3105330733739393
2201	80-81	-0.27518873155478474
2201	82-83	-0.3006313017725333
2201	84-85	-0.2508491612461583
2201	86-87	-0.36209330947338003
2201	88-89	-0.4160845448328061
2201	90-91	-0.32599453166445613
2201	92-93	-0.4568181083180676
2201	94-95	-0.41050879838699217
2201	96-97	-0.4115685732161012
2201	98-99	-0.4164807456801469
2201	100-101	-0.4840857082562877
1101	1	0.11404734837357466
1101	2	0.11480423886421676
1101	3	0.07678987666806592
1101	4	0.03880247633114209
1101	5	0.0732679659140274
1101	6	0.07932429444623779
1101	7	0.07560470270368569
1101	8	0.08293437726108266
1101	9	0.010498630565351164
1101	10-11	0.04445009200995287
1101	12-13	0.09429525696705099
1101	14-15	0.06233339770560775
1101	16-17	0.019442450610171136
1101	18-19	-0.06991790413734122
1101	20-21	-0.0521751544857878
1101	22-23	-0.06601772267170958
1101	24-25	-0.0433786041584483
1101	26-27	0.01586892951892338
1101	28-29	-0.07122707240191772
1101	30-31	-0.10080933658277758
1101	32-33	-0.141105634538647
1101	34-35	-0.08361557546432863
1101	36-37	-0.08037022608225186
1101	38-39	-0.06042260958488299
1101	40-41	-0.06579987648391494
1101	42-43	-0.09229718406383824
1101	44-45	-0.11205757998348531
1101	46-47	-0.13294822685023888
1101	48-49	-0.09380354379884182
1101	50-51	-0.18012803896451146
1101	52-53	-0.12279488144463357
1101	54-55	-0.20329959240159923
1101	56-57	-0.12602777341662375
1101	58-59	-0.14412046882056728
1101	60-61	-0.10014882736665243
1101	62-63	-0.15027173729567522
1101	64-65	-0.1247374440829887
1101	66-67	-0.15565303090731675
1101	68-69	-0.18105446816755943
1101	70-71	-0.18539469051048485
1101	72-73	-0.17657734957271032
1101	74-75	-0.14870107783529107
1101	76-77	-0.1685133367012881
1101	78-79	-0.20985451127603483
1101	80-81	-0.16843013967644538
1101	82-83	-0.2401683023373593
1101	84-85	-0.22188435596293488
1101	86-87	-0.22291782399779692
1101	88-89	-0.2922593755662035
1101	90-91	-0.325793821756875
1101	92-93	-0.3408560542285102
1101	94-95	-0.26354759439394115
1101	96-97	-0.26795966866679777
1101	98-99	-0.2975250462943606
1101	100-101	-0.37639575497621536
2202	1	0.0754975758113332
2202	2	0.12244688324066999
2202	3	0.06688227820335158
2202	4	0.08053768118445959
2202	5	0.058391687272731474
2202	6	0.007034318864270972
2202	7	0.041637642003991004
2202	8	0.00791225303937182
2202	9	-0.03185205815072578
2202	10-11	0.015098498414388928
2202	12-13	0.0015643527144106883
2202	14-15	0.012183610547445767
2202	16-17	0.0015805687570704663
2202	18-19	-0.00426218090889563
2202	20-21	-0.02650201613150216
2202	22-23	-0.08763584923880074
2202	24-25	-0.0359505280698329
2202	26-27	-0.04854731534262413
2202	28-29	-0.06626894698104735
2202	30-31	-0.06022102810165819
2202	32-33	-0.06727928208675138
2202	34-35	-0.10163476439768004
2202	36-37	-0.040727194343539
2202	38-39	-0.0957911369095541
2202	40-41	-0.12696945014597816
2202	42-43	-0.1639925395326003
2202	44-45	-0.1166313195885138
2202	46-47	-0.11022136331229149
2202	48-49	-0.1205525026418286
2202	50-51	-0.2034781714240239
2202	52-53	-0.17201396982837025
2202	54-55	-0.18644405715678403
2202	56-57	-0.14623878993879202
2202	58-59	-0.23795472018792196
2202	60-61	-0.1190867208565507
2202	62-63	-0.1591567688709148
2202	64-65	-0.14617392794670536
2202	66-67	-0.13851031970575178
2202	68-69	-0.17600059185465966
2202	70-71	-0.2565435964863241
2202	72-73	-0.23581498683756053
2202	74-75	-0.2556092087397772
2202	76-77	-0.18876910918455536
2202	78-79	-0.1606321486684692
2202	80-81	-0.20215204579245238
2202	82-83	-0.2501610982387277
2202	84-85	-0.3079133102285567
2202	86-87	-0.3030005212173563
2202	88-89	-0.34307360260500985
2202	90-91	-0.3121136815776495
2202	92-93	-0.30536459137185545
2202	94-95	-0.27504609634811317
2202	96-97	-0.30369155551687754
2202	98-99	-0.35514280227860695
2202	100-101	-0.37954405252376944
1102	1	0.09694311125723232
1102	2	0.09337224094189622
1102	3	0.02980384070499298
1102	4	0.01839268562146401
1102	5	0.05213307845944826
1102	6	-0.04931888356264835
1102	7	-0.03860498130713097
1102	8	-0.03939905267291266
1102	9	-0.05916559470399818
1102	10-11	-0.01003730099948541
1102	12-13	-0.015816184758968177
1102	14-15	-0.026196286564889704
1102	16-17	-0.005789724218033143
1102	18-19	-0.005902894598023067
1102	20-21	-0.048374273349061525
1102	22-23	-0.13605059534021535
1102	24-25	-0.09826261222254118
1102	26-27	-0.06694580908715153
1102	28-29	-0.1197816979185049
1102	30-31	-0.10800524480985274
1102	32-33	-0.11321192715621464
1102	34-35	-0.11072844849235963
1102	36-37	-0.13147416852257265
1102	38-39	-0.13416473197474232
1102	40-41	-0.12469923563281782
1102	42-43	-0.15163363151196307
1102	44-45	-0.1844425087034125
1102	46-47	-0.2034609753330514
1102	48-49	-0.21250423190808476
1102	50-51	-0.1743009461544318
1102	52-53	-0.1888678938339865
1102	54-55	-0.2748518075673232
1102	56-57	-0.2554554206447648
1102	58-59	-0.20941787625404373
1102	60-61	-0.15822722521726007
1102	62-63	-0.2620100504608871
1102	64-65	-0.26297546746948086
1102	66-67	-0.22445457210861974
1102	68-69	-0.2547253844206665
1102	70-71	-0.3414423267104496
1102	72-73	-0.2895020707385214
1102	74-75	-0.2632963314600332
1102	76-77	-0.3105165128159584
1102	78-79	-0.3744539197335044
1102	80-81	-0.37178647231887396
1102	82-83	-0.3474906488150822
1102	84-85	-0.379600610922985
1102	86-87	-0.3777028828482045
1102	88-89	-0.4362419313574506
1102	90-91	-0.4127436125997974
1102	92-93	-0.6043678845028495
1102	94-95	-0.5496159138406753
1102	96-97	-0.4792852493239863
1102	98-99	-0.4901290352035801
1102	100-101	-0.4687886777628023
2203	1	0.02805076563452502
2203	2	0.04959280173834202
2203	3	0.03711704518233461
2203	4	0.024158075130884527
2203	5	0.021277134059154434
2203	6	-0.08741677985269547
2203	7	-0.08834444985132706
2203	8	-0.10206751880779308
2203	9	-0.08897470471038815
2203	10-11	-0.02086397851288524
2203	12-13	-0.0494508382507135
2203	14-15	-0.027369656704500755
2203	16-17	-0.04301267730875935
2203	18-19	-0.038586331718470035
2203	20-21	-0.04744574888462694
2203	22-23	-0.10186472706559613
2203	24-25	-0.04785177524857431
2203	26-27	-0.04682889470995377
2203	28-29	-0.0785449436100194
2203	30-31	-0.13109698308367257
2203	32-33	-0.10082118159659359
2203	34-35	-0.09126205435550361
2203	36-37	-0.11132469125901423
2203	38-39	-0.11750700024640537
2203	40-41	-0.1715962875570085
2203	42-43	-0.09422665272946062
2203	44-45	-0.10657806675129677
2203	46-47	-0.0690054988398856
2203	48-49	-0.10731995376885095
2203	50-51	-0.1555682097317117
2203	52-53	-0.09113022021962536
2203	54-55	-0.13883135535166247
2203	56-57	-0.19617474274674152
2203	58-59	-0.22797072258502027
2203	60-61	-0.17768343636091544
2203	62-63	-0.2556765406979764
2203	64-65	-0.2748094954588183
2203	66-67	-0.22862959932472648
2203	68-69	-0.3136857660531547
2203	70-71	-0.2770674008137064
2203	72-73	-0.2828175739060299
2203	74-75	-0.2396198227460573
2203	76-77	-0.28366597362789037
2203	78-79	-0.2278818442806383
2203	80-81	-0.29526686622768494
2203	82-83	-0.3578078930191424
2203	84-85	-0.4052657532608137
2203	86-87	-0.41939058207606195
2203	88-89	-0.34774309743781373
2203	90-91	-0.3849999483220188
2203	92-93	-0.5491027380804852
2203	94-95	-0.4993184513064364
2203	96-97	-0.5078939858215961
2203	98-99	-0.5260044294045514
2203	100-101	-0.49970827296187537
1103	1	0.046016180721551336
1103	2	0.018348165122603177
1103	3	0.008342480453265466
1103	4	0.014201893736178306
1103	5	-0.0015306778813126698
1103	6	-0.10065494696476662
1103	7	-0.06536220399242865
1103	8	-0.07659538413793854
1103	9	-0.0641484194662425
1103	10-11	-0.008863214957557375
1103	12-13	-0.04678748032379332
1103	14-15	-0.06882030528259975
1103	16-17	-0.03784344326129485
1103	18-19	-0.0028807858799950736
1103	20-21	-0.013257138880874209
1103	22-23	-0.10854840503466079
1103	24-25	-0.0906217231314912
1103	26-27	-0.10124895076319973
1103	28-29	-0.09021678055248117
1103	30-31	-0.055120339590025935
1103	32-33	-0.07198513586614297
1103	34-35	-0.11036960615444258
1103	36-37	-0.12444798768716936
1103	38-39	-0.07947760632585243
1103	40-41	-0.06288703936305495
1103	42-43	-0.0894698806152121
1103	44-45	-0.06458804032281762
1103	46-47	-0.055177233679017945
1103	48-49	-0.1564847544601946
1103	50-51	-0.14137082180121752
1103	52-53	-0.12789888959129314
1103	54-55	-0.08853201206842698
1103	56-57	-0.1149403401400022
1103	58-59	-0.18041841460586738
1103	60-61	-0.1814816091552558
1103	62-63	-0.13922211873598656
1103	64-65	-0.1931960320251207
1103	66-67	-0.13880163603457873
1103	68-69	-0.2531191192152562
1103	70-71	-0.17298421471632963
1103	72-73	-0.13657787672377708
1103	74-75	-0.06486753725922512
1103	76-77	-0.09600546571562774
1103	78-79	-0.13522193123124993
1103	80-81	-0.265512636338336
1103	82-83	-0.2886554631078937
1103	84-85	-0.32369792557570776
1103	86-87	-0.2072892955741139
1103	88-89	-0.27503235764741163
1103	90-91	-0.26874483205740773
1103	92-93	-0.29302244047147497
1103	94-95	-0.25578584187090314
1103	96-97	-0.28175816175894397
1103	98-99	-0.30684693280059605
1103	100-101	-0.3381155202265447
2204	1	0.02736778878254853
2204	2	0.05838284695580853
2204	3	0.06064656001215951
2204	4	0.04462614846359969
2204	5	0.06463017445812369
2204	6	-0.015048232547428597
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1212	98-99	0.27517989161954404
1212	100-101	0.2230370696907329
1213	1	-0.25169054724621276
1213	2	-0.16316662444992147
1213	3	-0.17202963905185698
1213	4	-0.1666231339089208
1213	5	-0.16807597002974717
1213	6	-0.09979915252510096
1213	7	-0.06800360767850577
1213	8	-0.07155098561791817
1213	9	-0.10369352212453009
1213	10-11	-0.11247717637807852
1213	12-13	-0.16903837417462597
1213	14-15	-0.1796099703298566
1213	16-17	-0.27191313471261935
1213	18-19	-0.31791227593976146
1213	20-21	-0.19364881056651484
1213	22-23	-0.26390804576845284
1213	24-25	-0.30934973004210065
1213	26-27	-0.17307216802122127
1213	28-29	-0.18001046225707285
1213	30-31	-0.1599366435713634
1213	32-33	-0.14426951876434657
1213	34-35	-0.1659188971677139
1213	36-37	-0.08786139024520168
1213	38-39	-0.06080898189087236
1213	40-41	-0.01031557978183173
1213	42-43	6.322483466192352E-4
1213	44-45	0.06196007371644896
1213	46-47	0.0757662030673032
1213	48-49	0.08065676977008707
1213	50-51	0.08332322811921244
1213	52-53	0.08297553893578424
1213	54-55	0.07893500463605818
1213	56-57	0.09920849058124759
1213	58-59	0.12104307396823089
1213	60-61	0.1351795637798574
1213	62-63	0.14905286972112464
1213	64-65	0.1360680576348514
1213	66-67	0.1634080859664735
1213	68-69	0.17608320911156738
1213	70-71	0.13086284449444463
1213	72-73	0.11129915751521935
1213	74-75	0.17294100296383164
1213	76-77	0.21691938498304353
1213	78-79	0.2381422590453255
1213	80-81	0.2249819258282102
1213	82-83	0.21661136125964475
1213	84-85	0.2599359670056103
1213	86-87	0.24057047104163587
1213	88-89	0.25967974632485635
1213	90-91	0.2711469183600599
1213	92-93	0.3227000641078632
1213	94-95	0.25174495739497615
1213	96-97	0.30241563362194057
1213	98-99	0.3446127444967928
1213	100-101	0.13500016039449036
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	17.0
14	45.0
15	87.0
16	154.0
17	233.0
18	530.0
19	1269.0
20	2968.0
21	6431.0
22	12136.0
23	20525.0
24	31241.0
25	44631.0
26	59841.0
27	79334.0
28	100892.0
29	126163.0
30	154763.0
31	188825.0
32	234897.0
33	278941.0
34	332373.0
35	430812.0
36	604514.0
37	982625.0
38	2207722.0
39	8282148.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	18.477640018935464	30.836259982553354	29.154598273779698	21.531501724731488
2	20.336949380393765	31.968362578661203	29.52842441978473	18.1662636211603
3	20.00212632199805	30.066841665223148	30.18914043080722	19.741891581971583
4	19.907111595314674	29.656389608179346	29.86577874392886	20.57072005257712
5	20.79432916927	29.89909855946336	29.869536544023433	19.437035727243206
6	20.666919202654633	29.992617799190462	29.21628466544657	20.12417833270834
7	20.392295128417228	30.579027231656365	29.478479344184766	19.550198295741637
8	19.716919689381818	30.489346452810334	29.857956464216063	19.935777393591785
9	20.1392227545036	29.702950611766365	30.25216233874599	19.905664294984046
10-11	20.097779086283623	30.200195049152512	29.832357558810322	19.869668305753542
12-13	19.70585517877181	30.254070023399088	29.98238908541369	20.05768571241541
14-15	19.69518205384307	30.127541644772982	30.087137171389344	20.090139129994608
16-17	19.978454448625826	29.81582818973833	30.074783720488774	20.130933641147067
18-19	19.735955364722386	29.694389858741292	30.329385325854265	20.240269450682057
20-21	20.069883174125735	29.65411413807615	30.22251815606784	20.05348453173028
22-23	19.90667502391676	30.061682131703677	29.967886910487007	20.06375593389256
24-25	19.938058137886298	29.61776867588492	30.107700823181887	20.336472363046894
26-27	20.035820843328565	29.597166566850973	29.8089845262939	20.558028063526564
28-29	20.24762751866837	29.854167589075036	29.780918964086283	20.117285928170308
30-31	20.122142394692865	29.525691083747603	30.01448204226055	20.337684479298986
32-33	20.16445913939767	29.805109814216042	29.70711003552633	20.323321010859964
34-35	20.384453914894202	29.8021284223022	29.689341774599935	20.124075888203663
36-37	20.158938890109816	29.569278047310522	29.90289136003072	20.368891702548943
38-39	20.401308009777814	29.740480478581226	29.69908914746514	20.159122364175815
40-41	20.240351262763916	29.760177552037316	29.825507332581285	20.173963852617483
42-43	20.219785837673044	29.514736859362696	29.88748923996754	20.377988062996717
44-45	20.418932132756172	29.42907081622776	29.6897686390112	20.46222841200487
46-47	20.238919927002428	29.706484903468738	29.846203128158137	20.208392041370697
48-49	20.430224402517815	29.418054796473637	29.87842804078502	20.273292760223526
50-51	20.218501963220632	29.453484910966104	29.61492051206579	20.713092613747477
52-53	20.456673912017205	29.467903388572562	29.874034837125222	20.20138786228501
54-55	20.25711089052956	29.40782667296329	29.887515748165438	20.447546688341713
56-57	20.266524802142353	29.45249248283718	29.829921216537652	20.45106149848282
58-59	20.432064216878054	29.4406067346554	29.880617824036175	20.24671122443037
60-61	20.250168903228012	29.413531682332554	29.801156879151304	20.535142535288127
62-63	20.437440788783732	29.397528353084358	29.87216281883725	20.292868039294657
64-65	20.270047044187216	29.840839250194882	29.627615086655652	20.26149861896225
66-67	20.257692847852596	29.866004400505812	29.575419858565645	20.300882893075944
68-69	20.20365379024878	29.83252057066438	29.61922192936929	20.34460370971755
70-71	20.326238469069732	29.853494167506824	29.53792519206481	20.28234217135864
72-73	20.29550012935454	29.875258792803987	29.551440611953616	20.277800465887854
74-75	20.298530291095382	29.75770134750377	29.614221377528295	20.329546983872554
76-77	20.302011931837097	29.77450150953424	29.595341291276632	20.328145267352028
78-79	20.313974199395837	29.740965308254914	29.619999299657486	20.325061192691766
80-81	20.36157981648135	29.716017828823706	29.576953821970076	20.345448532724873
82-83	20.315571065365074	29.7778291730658	29.616706355772664	20.28989340579646
84-85	20.339310410480028	29.702619918360583	29.586724031093848	20.371345640065545
86-87	20.294293640403545	29.709979476347172	29.611479199299716	20.384247683949567
88-89	20.35309663812513	29.68250565695013	29.577345263287047	20.38705244163769
90-91	20.3454855730168	29.672440458782322	29.559673909089035	20.42240005911184
92-93	20.310252471045647	29.682481043852267	29.58873598204766	20.418530503054427
94-95	20.341657950261027	29.680258524137	29.538732256064808	20.439351269537166
96-97	20.385042757732553	29.723983625559296	29.470644033741383	20.42032958296677
98-99	20.37274230996281	29.709990687117827	29.499927345559257	20.417339657360106
100-101	20.45015601795658	29.737287962028198	29.38016636862764	20.43238965138758
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1029.0
1	983.5
2	989.0
3	1210.5
4	1532.5
5	1841.5
6	2384.5
7	3064.5
8	3873.5
9	4793.0
10	5647.5
11	6874.0
12	8447.0
13	10042.0
14	11742.5
15	13979.0
16	17074.5
17	21566.0
18	28297.5
19	37563.0
20	50435.0
21	67187.5
22	87146.5
23	110384.0
24	137111.0
25	167641.0
26	202003.0
27	238501.0
28	276238.5
29	314267.0
30	351627.0
31	387564.5
32	422401.5
33	456742.0
34	488329.5
35	516464.5
36	542846.5
37	564879.0
38	576569.5
39	575591.5
40	565904.5
41	547736.0
42	520092.5
43	487514.0
44	455534.5
45	429071.0
46	407085.0
47	390353.5
48	373368.5
49	350606.0
50	326579.0
51	301400.0
52	274349.0
53	250704.0
54	230230.0
55	210365.5
56	192636.0
57	176061.0
58	158233.5
59	138892.0
60	119226.0
61	99714.5
62	82250.0
63	67935.5
64	56005.0
65	45890.0
66	37646.0
67	30964.0
68	25234.0
69	20327.0
70	16232.5
71	12916.0
72	10516.5
73	8767.0
74	7370.0
75	6182.0
76	5282.0
77	4663.0
78	4243.5
79	3686.5
80	3143.0
81	2744.0
82	2360.5
83	1984.0
84	1634.0
85	1286.5
86	927.0
87	666.0
88	479.5
89	322.5
90	188.5
91	116.0
92	61.0
93	26.5
94	21.0
95	18.0
96	10.5
97	5.5
98	4.0
99	2.0
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.08295193842521181
2	1.9740389902311156E-4
3	0.0
4	0.0
5	0.00264380221905953
6	0.0
7	0.0
8	1.057520887623812E-4
9	0.003637871853425913
10-11	0.0
12-13	1.0927715838779389E-4
14-15	3.102061270363182E-4
16-17	0.001512254869302051
18-19	0.0
20-21	1.1280222801320659E-4
22-23	1.7977855089604803E-4
24-25	7.050139250825412E-5
26-27	0.0033523412137674838
28-29	0.001642682445442321
30-31	0.0024252479022839417
32-33	0.0029011323017146574
34-35	0.004938622545203201
36-37	0.006556629503267634
38-39	0.00842844147436178
40-41	0.010279103027703452
42-43	0.018221084893758278
44-45	0.011315473497574786
46-47	0.014110853710527064
48-49	0.015129598832271334
50-51	0.011854809150262931
52-53	0.016768756208088244
54-55	0.013462240899451125
56-57	0.011082818902297549
58-59	0.008312114176723161
60-61	0.004931572405952376
62-63	0.0017942604393350677
64-65	0.0015369303566799399
66-67	0.002065690800491846
68-69	0.010011197736172086
70-71	0.0048540208741932964
72-73	0.001632107236566083
74-75	0.021457098809887144
76-77	0.03460913358230195
78-79	0.03765126866903312
80-81	0.025355825815593595
82-83	0.04528304440805163
84-85	0.020124622491481142
86-87	0.017138888518756577
88-89	0.017251690746769785
90-91	0.005872765995937569
92-93	0.01811885787462131
94-95	0.02117861830947954
96-97	0.012478746473960982
98-99	0.003792974916944072
100-101	8.812674063531766E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	1.4184117E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	94.48504709259011
#Duplication Level	Percentage of deduplicated	Percentage of total
1	98.2602280332156	92.84122273047018
2	1.067632215859886	2.017505603861753
3	0.25050319383329367	0.7100641819854894
4	0.125287076449391	0.47351021273654575
5	0.055405764553494226	0.26175081365189307
6	0.037387490269715454	0.21195352672846915
7	0.03582673006817825	0.23695631943657344
8	0.029706883002389477	0.22454849915638672
9	0.02201132887566632	0.18717673008490385
>10	0.11382776896816767	2.2565035388974843
>50	0.001808052784184374	0.10130099996834377
>100	2.9412509218183325E-4	0.03885544027850368
>500	5.266601458252164E-5	0.02935253113944631
>1k	2.120564477505865E-5	0.044942224079211716
>5k	0.0	0.0
>10k+	7.465368461918003E-6	0.36435664752481384
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC	51655	0.3641749430013867	TruSeq Adapter, Index 9 (100% over 50bp)
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA Adapter	Nextera Transposase Sequence
1	4.230083550495247E-5	0.0	0.0
2	6.345125325742871E-5	0.0	0.0
3	9.165181026073037E-5	0.0	1.4100278501650826E-5
4	9.870194951155578E-5	0.0	2.1150417752476237E-5
5	1.128022280132066E-4	0.0	2.1150417752476237E-5
6	1.2690250651485743E-4	0.0	4.230083550495247E-5
7	1.5510306351815907E-4	0.0	4.230083550495247E-5
8	1.9035375977228614E-4	0.0	4.230083550495247E-5
9	2.0445403827393696E-4	0.0	4.935097475577789E-5
10-11	2.5380501302971485E-4	0.0	6.345125325742871E-5
12-13	3.0668105741090546E-4	0.0	9.517687988614307E-5
14-15	3.983328676716358E-4	0.0	1.3747771539109554E-4
16-17	5.146601653102552E-4	7.050139250825413E-6	1.4805292426733365E-4
18-19	5.675362096914456E-4	7.050139250825413E-6	1.692033420198099E-4
20-21	6.768133680792397E-4	7.050139250825413E-6	1.9387882939769885E-4
22-23	7.614150390891446E-4	7.050139250825413E-6	2.220793864010005E-4
24-25	8.495417797244622E-4	7.050139250825413E-6	2.5380501302971485E-4
26-27	9.447186596106053E-4	7.050139250825413E-6	2.9258077890925466E-4
28-29	0.0010962966535033518	7.050139250825413E-6	3.1725626628714357E-4
30-31	0.001261974925897749	7.050139250825413E-6	3.560320321666833E-4
32-33	0.0013536267361584794	7.050139250825413E-6	3.7718244991915954E-4
34-35	0.001501679660425813	1.057520887623812E-5	4.018579372970485E-4
36-37	0.0016532576543185594	1.4100278501650826E-5	4.300584943003502E-4
38-39	0.0017836852304588295	1.4100278501650826E-5	4.406337031765883E-4
40-41	0.0019634637813548774	1.4100278501650826E-5	4.6530919055447727E-4
42-43	0.0021256169841238617	1.4100278501650826E-5	4.864596083069535E-4
44-45	0.0023758969275281643	1.4100278501650826E-5	5.076100260594297E-4
46-47	0.002633227010183292	1.4100278501650826E-5	5.252353741864932E-4
48-49	0.002929332858717959	1.762534812706353E-5	5.428607223135568E-4
50-51	0.003179612802122261	2.1150417752476237E-5	5.569610008152076E-4
52-53	0.0035497451127905954	2.1150417752476237E-5	5.922116970693347E-4
54-55	0.003965703328589295	2.1150417752476237E-5	6.168871844472237E-4
56-57	0.004476838424274137	2.820055700330165E-5	6.48612811075938E-4
58-59	0.00511840109609925	3.525069625412706E-5	6.662381592030015E-4
60-61	0.0060525445468336166	3.525069625412706E-5	6.83863507330065E-4
62-63	0.007166466548464033	4.230083550495247E-5	6.873885769554777E-4
64-65	0.008629370443010306	4.935097475577789E-5	6.944387162063032E-4
66-67	0.010592834224365182	5.64011140066033E-5	7.050139250825413E-4
68-69	0.013070958171030315	5.64011140066033E-5	7.296894124604302E-4
70-71	0.01634574785303872	5.64011140066033E-5	7.543648998383191E-4
72-73	0.021227969284235315	6.345125325742871E-5	7.755153175907953E-4
74-75	0.028024303522031016	6.345125325742871E-5	8.072409442195097E-4
76-77	0.03698855557945553	6.345125325742871E-5	8.354415012228114E-4
78-79	0.04951312795854687	6.345125325742871E-5	8.530668493498749E-4
80-81	0.06647928806565823	6.345125325742871E-5	8.777423367277639E-4
82-83	0.08918778659256688	6.345125325742871E-5	8.847924759785894E-4
84-85	0.11873139512315078	6.697632288284143E-5	9.165181026073036E-4
86-87	0.15631568746930105	7.050139250825412E-5	9.447186596106053E-4
88-89	0.20533883074991555	7.755153175907954E-5	9.658690773630815E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGAAGA	11945	0.0	49.381424	4
TCGGAAG	12190	0.0	48.38893	3
ATCGGAA	12530	0.0	47.18762	2
GATCGGA	12755	0.0	46.504463	1
CGATCAG	7120	0.0	40.989185	32-33
TATGCCG	7095	0.0	40.406696	46-47
GTCACGA	7290	0.0	40.35829	28-29
TCTCGTA	6440	0.0	39.574844	40-41
CTCGTAT	6925	0.0	39.442833	42-43
ATGCCGT	7530	0.0	38.640217	46-47
AGAGCAC	15740	0.0	37.685177	8
GCCGTCT	7640	0.0	37.679058	48-49
CGTATGC	7680	0.0	37.604465	44-45
ACGATCA	7700	0.0	37.469933	32-33
TGCCGTC	7690	0.0	37.18698	48-49
AGTCACG	7985	0.0	36.340027	28-29
TCACGAT	7995	0.0	36.176155	30-31
TCGTATG	8150	0.0	36.04974	42-43
ATCTCGT	6955	0.0	35.92725	40-41
GAGCACA	16515	0.0	35.80195	9
>>END_MODULE