FastQCFastQC Report
Fri 3 Apr 2015
H2K5YBCXX l01n01 50617-bar10.341000000044bd.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2K5YBCXX l01n01 50617-bar10.341000000044bd.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14184117
Sequences flagged as poor quality0
Sequence length101
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC516550.3641749430013867TruSeq Adapter, Index 9 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGAAGA119450.049.3814244
TCGGAAG121900.048.388933
ATCGGAA125300.047.187622
GATCGGA127550.046.5044631
CGATCAG71200.040.98918532-33
TATGCCG70950.040.40669646-47
GTCACGA72900.040.3582928-29
TCTCGTA64400.039.57484440-41
CTCGTAT69250.039.44283342-43
ATGCCGT75300.038.64021746-47
AGAGCAC157400.037.6851778
GCCGTCT76400.037.67905848-49
CGTATGC76800.037.60446544-45
ACGATCA77000.037.46993332-33
TGCCGTC76900.037.1869848-49
AGTCACG79850.036.34002728-29
TCACGAT79950.036.17615530-31
TCGTATG81500.036.0497442-43
ATCTCGT69550.035.9272540-41
GAGCACA165150.035.801959
Produced by FastQC (version 0.11.2)