##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename H2K5YBCXX l01n01 50072-bar8.341000000044d7.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 13225565 Sequences flagged as poor quality 0 Sequence length 101 %GC 40 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.97100161694415 38.0 38.0 38.0 32.0 38.0 2 36.95502725214386 38.0 38.0 38.0 32.0 38.0 3 36.93720510239071 38.0 38.0 38.0 32.0 38.0 4 36.93064644119174 38.0 38.0 38.0 32.0 38.0 5 36.9278870883777 38.0 38.0 38.0 32.0 38.0 6 38.86494248071821 40.0 38.0 40.0 38.0 40.0 7 38.82337261205854 40.0 38.0 40.0 38.0 40.0 8 38.81297343440526 40.0 38.0 40.0 38.0 40.0 9 38.83190888253167 40.0 38.0 40.0 38.0 40.0 10-11 38.82933587336345 40.0 38.0 40.0 38.0 40.0 12-13 38.81804115740991 40.0 38.0 40.0 38.0 40.0 14-15 38.80759075321168 40.0 38.0 40.0 38.0 40.0 16-17 38.802048645936864 40.0 38.0 40.0 38.0 40.0 18-19 38.766373043420074 40.0 38.0 40.0 38.0 40.0 20-21 38.751394099231305 40.0 38.0 40.0 38.0 40.0 22-23 38.73647556834056 40.0 38.0 40.0 38.0 40.0 24-25 38.70433350106404 40.0 38.0 40.0 38.0 40.0 26-27 38.64006089720931 40.0 38.0 40.0 38.0 40.0 28-29 38.590450804937255 40.0 38.0 40.0 38.0 40.0 30-31 38.573351573259814 40.0 38.0 40.0 38.0 40.0 32-33 38.53674655865363 40.0 38.0 40.0 38.0 40.0 34-35 38.514754757169165 40.0 38.0 40.0 38.0 40.0 36-37 38.486923280782335 40.0 38.0 40.0 38.0 40.0 38-39 38.45769148614823 40.0 38.0 40.0 38.0 40.0 40-41 38.4235448542274 40.0 38.0 40.0 38.0 40.0 42-43 38.37213071804494 40.0 38.0 40.0 38.0 40.0 44-45 38.350900547538046 40.0 38.0 40.0 38.0 40.0 46-47 38.31481305335538 40.0 38.0 40.0 38.0 40.0 48-49 38.25208200935083 40.0 38.0 40.0 38.0 40.0 50-51 38.238224075871244 40.0 38.0 40.0 38.0 40.0 52-53 38.21205835062623 40.0 38.0 40.0 38.0 40.0 54-55 38.145891914636536 40.0 38.0 40.0 38.0 40.0 56-57 38.13802869669462 40.0 38.0 40.0 38.0 40.0 58-59 38.10930444181402 40.0 38.0 40.0 38.0 40.0 60-61 38.05249133023807 40.0 38.0 40.0 38.0 40.0 62-63 38.026083498134106 40.0 38.0 40.0 38.0 40.0 64-65 37.96392082304234 40.0 38.0 40.0 38.0 40.0 66-67 37.9121970214505 40.0 38.0 40.0 38.0 40.0 68-69 37.8702914771505 40.0 38.0 40.0 38.0 40.0 70-71 37.8522980303677 40.0 38.0 40.0 35.0 40.0 72-73 37.82264693417635 40.0 38.0 40.0 32.0 40.0 74-75 37.74234408889147 40.0 38.0 40.0 32.0 40.0 76-77 37.68012973358795 40.0 38.0 40.0 32.0 40.0 78-79 37.60703920777676 40.0 38.0 40.0 32.0 40.0 80-81 37.5755326521022 40.0 38.0 40.0 32.0 40.0 82-83 37.50372252527586 40.0 38.0 40.0 32.0 40.0 84-85 37.46945744850976 40.0 38.0 40.0 32.0 40.0 86-87 37.43027753445694 40.0 38.0 40.0 32.0 40.0 88-89 37.36379515733354 40.0 38.0 40.0 32.0 40.0 90-91 37.30685241046413 38.0 38.0 40.0 32.0 40.0 92-93 37.22871552179434 38.0 38.0 40.0 32.0 40.0 94-95 37.15798825985884 38.0 38.0 40.0 32.0 40.0 96-97 37.07607039850471 38.0 38.0 40.0 32.0 40.0 98-99 37.057536483318486 38.0 38.0 40.0 32.0 40.0 100-101 36.23372283150096 38.0 38.0 40.0 29.5 40.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2201 1 0.09762569942148502 2201 2 0.09269836018922462 2201 3 0.06280757209542287 2201 4 0.08108958635460084 2201 5 0.021434655946691805 2201 6 0.020938892856818825 2201 7 0.045681530699404505 2201 8 -0.034358932957175625 2201 9 -0.004333515716389513 2201 10-11 -0.0012642808726042176 2201 12-13 0.007896576245329356 2201 14-15 -0.027379867332776087 2201 16-17 -0.12204996737598606 2201 18-19 -0.08596469823140751 2201 20-21 -0.04576521615030771 2201 22-23 -0.07497747703612845 2201 24-25 -0.0782000712975659 2201 26-27 -0.10661573320606976 2201 28-29 -0.11250370555359268 2201 30-31 -0.11519099263344401 2201 32-33 -0.1621578040794205 2201 34-35 -0.15039033118325307 2201 36-37 -0.12854469832342375 2201 38-39 -0.12711801099994346 2201 40-41 -0.1851083373214948 2201 42-43 -0.14613994156017895 2201 44-45 -0.18196263545474523 2201 46-47 -0.15053502058168533 2201 48-49 -0.1194540351100386 2201 50-51 -0.23614312705253582 2201 52-53 -0.17271136235664386 2201 54-55 -0.17633203039195422 2201 56-57 -0.22890631493677205 2201 58-59 -0.17578348985896497 2201 60-61 -0.1659401319046978 2201 62-63 -0.16812990328202204 2201 64-65 -0.2882144144392953 2201 66-67 -0.21583535373166995 2201 68-69 -0.24527101869403367 2201 70-71 -0.24288389471657013 2201 72-73 -0.21628772366872084 2201 74-75 -0.1539187226715768 2201 76-77 -0.24067915775384563 2201 78-79 -0.2697139098272672 2201 80-81 -0.270207786583363 2201 82-83 -0.296714321806661 2201 84-85 -0.3195827273007623 2201 86-87 -0.37680017751094397 2201 88-89 -0.36394903536567114 2201 90-91 -0.2948219708149722 2201 92-93 -0.44210545942613066 2201 94-95 -0.3385794964811595 2201 96-97 -0.40681843645685944 2201 98-99 -0.3572444734174596 2201 100-101 -0.4595412636271803 1101 1 0.09301513527724836 1101 2 0.11475781481978231 1101 3 0.09642773935544113 1101 4 0.05972478025295658 1101 5 0.09667421588675751 1101 6 0.09557940044490465 1101 7 0.0905840833474656 1101 8 0.10078623732781722 1101 9 0.028031464661331995 1101 10-11 0.055278849645198136 1101 12-13 0.07574633324081503 1101 14-15 0.0429520139436832 1101 16-17 0.04556610072349798 1101 18-19 -0.0398527518044105 1101 20-21 -0.03772640330804222 1101 22-23 -0.06403594384583045 1101 24-25 -0.01937990188628902 1101 26-27 0.058102732616660546 1101 28-29 -0.02302483119586185 1101 30-31 -0.04266525097321505 1101 32-33 -0.03962003534577008 1101 34-35 -0.031289179438466874 1101 36-37 -0.014765325598901313 1101 38-39 -0.0013851786959975243 1101 40-41 -0.020868348900975775 1101 42-43 -9.447676400426985E-4 1101 44-45 -0.040410383925504334 1101 46-47 -0.04859865041089506 1101 48-49 -0.03986963460288706 1101 50-51 -0.11238261373498659 1101 52-53 -0.07966495430923004 1101 54-55 -0.14325361735986064 1101 56-57 -0.04140301073075392 1101 58-59 -0.08709317519993931 1101 60-61 -0.05769910338869266 1101 62-63 -0.10388486835181254 1101 64-65 -0.09389395751756524 1101 66-67 -0.08557845852008938 1101 68-69 -0.10225411608318069 1101 70-71 -0.1698092568570715 1101 72-73 -0.15651004146189962 1101 74-75 -0.11025508216425095 1101 76-77 -0.13799928488074897 1101 78-79 -0.16218824013844113 1101 80-81 -0.12134630193185814 1101 82-83 -0.17131049463434067 1101 84-85 -0.21888094405124292 1101 86-87 -0.20624305002814225 1101 88-89 -0.23127234064617852 1101 90-91 -0.24323948871288792 1101 92-93 -0.3261180999997819 1101 94-95 -0.23074077327181897 1101 96-97 -0.26945969640615175 1101 98-99 -0.2942278118461701 1101 100-101 -0.31717809965248733 2202 1 0.06502977805942578 2202 2 0.0804945112864317 2202 3 0.04797267948295314 2202 4 0.06977043276533124 2202 5 0.07227116081819673 2202 6 -0.04316825334252883 2202 7 -0.02881992103894504 2202 8 -0.01295483538501685 2202 9 0.009051552508438476 2202 10-11 0.00918264460695184 2202 12-13 -0.005325613843780275 2202 14-15 -0.0072778285291761335 2202 16-17 -0.03749169911055361 2202 18-19 -0.008413732965159681 2202 20-21 -0.0827122647738392 2202 22-23 -0.11216862734805488 2202 24-25 -0.03813630730757467 2202 26-27 -0.08729611102918966 2202 28-29 -0.06172912745562087 2202 30-31 -0.0850940205064461 2202 32-33 -0.06656095810894413 2202 34-35 -0.11284538117166676 2202 36-37 -0.08642157540551665 2202 38-39 -0.11591812367409204 2202 40-41 -0.16626452756484866 2202 42-43 -0.14736906594164623 2202 44-45 -0.11768987088678529 2202 46-47 -0.1200138803213946 2202 48-49 -0.1325831708738221 2202 50-51 -0.22035584868314828 2202 52-53 -0.17584981441430614 2202 54-55 -0.181797114450319 2202 56-57 -0.15915999664115787 2202 58-59 -0.19976815079151322 2202 60-61 -0.15598322106317397 2202 62-63 -0.15213425362036048 2202 64-65 -0.14701987226368374 2202 66-67 -0.15587067874137261 2202 68-69 -0.21100086950018948 2202 70-71 -0.2725685559360542 2202 72-73 -0.24154964589605754 2202 74-75 -0.250861054111013 2202 76-77 -0.2689519081429239 2202 78-79 -0.22194980199576975 2202 80-81 -0.21925185206146836 2202 82-83 -0.2657548889985861 2202 84-85 -0.3185177725901056 2202 86-87 -0.2824007137239448 2202 88-89 -0.37167030985598615 2202 90-91 -0.3269767426913788 2202 92-93 -0.37351128379626175 2202 94-95 -0.31585545626958833 2202 96-97 -0.3288578787310499 2202 98-99 -0.3881793157086051 2202 100-101 -0.39358737162913116 1102 1 0.10810260945692818 1102 2 0.07089927260069118 1102 3 0.041401762469554626 1102 4 0.07480108418056375 1102 5 0.04837721404670958 1102 6 -0.09066098485268981 1102 7 -0.11328000787408854 1102 8 -0.05715507714886314 1102 9 -0.0026218113105684893 1102 10-11 -0.03707010168791669 1102 12-13 -0.03000649972157987 1102 14-15 -0.03400155062286814 1102 16-17 -0.016034098500959715 1102 18-19 0.026420920727446173 1102 20-21 -0.018195191256431542 1102 22-23 -0.15758572942550586 1102 24-25 -0.0629914652143242 1102 26-27 -0.06735148776819955 1102 28-29 -0.13746606859137955 1102 30-31 -0.10656976038702481 1102 32-33 -0.09467320720693806 1102 34-35 -0.12984699643522646 1102 36-37 -0.1690530119514193 1102 38-39 -0.12628655635924702 1102 40-41 -0.15247732557039484 1102 42-43 -0.168756443596493 1102 44-45 -0.15267447723263672 1102 46-47 -0.21361600393233005 1102 48-49 -0.1820509392156211 1102 50-51 -0.18608480784723014 1102 52-53 -0.16874070029706445 1102 54-55 -0.2515328796004326 1102 56-57 -0.28295915064656896 1102 58-59 -0.27405230279752857 1102 60-61 -0.20424155848898096 1102 62-63 -0.29138998805068184 1102 64-65 -0.23696320364619794 1102 66-67 -0.2663023671931839 1102 68-69 -0.2807888423977971 1102 70-71 -0.32263288615228447 1102 72-73 -0.2781641841775553 1102 74-75 -0.2857088325918795 1102 76-77 -0.32313833359308575 1102 78-79 -0.3734147228269009 1102 80-81 -0.3846945281162917 1102 82-83 -0.4398062576367323 1102 84-85 -0.4194897865022753 1102 86-87 -0.4255498189238338 1102 88-89 -0.45813156480594586 1102 90-91 -0.394553172900622 1102 92-93 -0.5566462609651381 1102 94-95 -0.5699474876887081 1102 96-97 -0.5361193558000821 1102 98-99 -0.5359589523777757 1102 100-101 -0.5760000122710878 2203 1 0.060954778882823746 2203 2 0.03909955799424836 2203 3 0.027854813120818278 2203 4 0.07181307056626451 2203 5 0.04917528022404127 2203 6 -0.09269175383853678 2203 7 -0.05364639997388565 2203 8 -0.10665682738718374 2203 9 -0.039635247980768895 2203 10-11 -0.024963534350064265 2203 12-13 -0.07132441151055247 2203 14-15 -0.05944349177043051 2203 16-17 -0.037194893775705395 2203 18-19 -0.032068295753973075 2203 20-21 -0.04037326902500382 2203 22-23 -0.11044725646962661 2203 24-25 -0.07865168387863264 2203 26-27 -0.08415391278123252 2203 28-29 -0.122670130137152 2203 30-31 -0.14786987110151983 2203 32-33 -0.11612051421763425 2203 34-35 -0.13299757619535058 2203 36-37 -0.14585935738146105 2203 38-39 -0.15674603149550137 2203 40-41 -0.2047754568288127 2203 42-43 -0.13995308293868902 2203 44-45 -0.16834560034409662 2203 46-47 -0.14948065563218904 2203 48-49 -0.14718006684986307 2203 50-51 -0.18280758878498204 2203 52-53 -0.1728051270762947 2203 54-55 -0.18652069251159276 2203 56-57 -0.18980714234115936 2203 58-59 -0.2679512360502372 2203 60-61 -0.23996265443116016 2203 62-63 -0.319357245868531 2203 64-65 -0.3098957219996805 2203 66-67 -0.27503375660459284 2203 68-69 -0.31060139883928883 2203 70-71 -0.30000987767571274 2203 72-73 -0.32874843456165337 2203 74-75 -0.31811623217997465 2203 76-77 -0.3219157269787658 2203 78-79 -0.3065204922630045 2203 80-81 -0.34580476845096797 2203 82-83 -0.35602096318825716 2203 84-85 -0.4571175873893054 2203 86-87 -0.43074726305185607 2203 88-89 -0.4006042390139015 2203 90-91 -0.430758328748432 2203 92-93 -0.5752297340663048 2203 94-95 -0.5017550398404111 2203 96-97 -0.5432333613783555 2203 98-99 -0.5077821577692845 2203 100-101 -0.5499890805551573 1103 1 0.07581221585921583 1103 2 0.0368206138796765 1103 3 0.04889503224943326 1103 4 0.0557020890370481 1103 5 0.04757439973536748 1103 6 -0.06367707092037733 1103 7 -0.03616916916563184 1103 8 -0.020393109729482717 1103 9 -0.02768786846008453 1103 10-11 0.025088669558002152 1103 12-13 -0.025813148314895784 1103 14-15 -0.023439773436102485 1103 16-17 0.004872916658129611 1103 18-19 0.01527119282980749 1103 20-21 -3.619652130026907E-4 1103 22-23 -0.08560106293103331 1103 24-25 -0.014735951804468073 1103 26-27 -0.024564408350556732 1103 28-29 -0.07160752270849002 1103 30-31 -0.029148545370162537 1103 32-33 -0.06045910302292157 1103 34-35 -0.059195782457784674 1103 36-37 -0.10022700252251582 1103 38-39 -0.06917246500751162 1103 40-41 -0.009281391462351962 1103 42-43 -0.03170335370359112 1103 44-45 -0.03615589019450027 1103 46-47 -0.028199166269885723 1103 48-49 -0.09800734529282096 1103 50-51 -0.06930701851066345 1103 52-53 -0.07715158889001117 1103 54-55 -0.045582214209240135 1103 56-57 -0.08473981694182697 1103 58-59 -0.14458960864538284 1103 60-61 -0.12746855934776136 1103 62-63 -0.07273262672818248 1103 64-65 -0.13096464985831346 1103 66-67 0.0015314878833194712 1103 68-69 -0.17213873794793955 1103 70-71 -0.11260816873129897 1103 72-73 -0.11838033599438802 1103 74-75 -0.06841886717047885 1103 76-77 -0.13873644080218384 1103 78-79 -0.153304772291456 1103 80-81 -0.19114329226584204 1103 82-83 -0.24056905893706926 1103 84-85 -0.26869783331465413 1103 86-87 -0.232637870475223 1103 88-89 -0.2819868129937575 1103 90-91 -0.2546474152871099 1103 92-93 -0.3135602427957096 1103 94-95 -0.2607594552989667 1103 96-97 -0.22552949065957506 1103 98-99 -0.22624328595757248 1103 100-101 -0.2210557369015831 2204 1 0.041572103864034204 2204 2 0.13270918485704186 2204 3 0.07094608555360793 2204 4 0.0697184558083137 2204 5 0.08252223814555748 2204 6 0.029985159799139183 2204 7 0.019756467772971575 2204 8 0.03436858054404013 2204 9 0.05497459299383678 2204 10-11 0.03499269310650277 2204 12-13 0.05874081748528681 2204 14-15 0.043543776503334186 2204 16-17 0.05962623350565366 2204 18-19 0.060799264558440314 2204 20-21 0.09245955027677866 2204 22-23 0.007798461660627254 2204 24-25 -0.008221709537231447 2204 26-27 0.07530910437526472 2204 28-29 -0.015787954222275857 2204 30-31 -0.014276931227662715 2204 32-33 0.005448421224414801 2204 34-35 0.04792457146387363 2204 36-37 0.008405766075149756 2204 38-39 0.04448709976688292 2204 40-41 0.08979888578623729 2204 42-43 0.09595415225975046 2204 44-45 0.05275684329464525 2204 46-47 0.11056826299611089 2204 48-49 0.052777462149123266 2204 50-51 0.05023049411419578 2204 52-53 0.07196624944412378 2204 54-55 0.008100977784437191 2204 56-57 0.05663299674450428 2204 58-59 0.04044905413223887 2204 60-61 0.06668503776278101 2204 62-63 0.025191925588828212 2204 64-65 0.019255917104331388 2204 66-67 0.07666581968231156 2204 68-69 0.030244222793555764 2204 70-71 0.024897993018051068 2204 72-73 0.014402237603583501 2204 74-75 0.033714297486191924 2204 76-77 0.009351764462763867 2204 78-79 0.051323601511548134 2204 80-81 0.06767240332880675 2204 82-83 -0.04170742215638512 2204 84-85 -0.01682374832382294 2204 86-87 0.0317331988687215 2204 88-89 0.005309918045000472 2204 90-91 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0.5169504894542669 1214 92-93 0.5679537430545878 1214 94-95 0.5225047993775078 1214 96-97 0.5141447899031917 1214 98-99 0.5702880075097809 1214 100-101 0.6889659603158478 1215 1 -0.14656584022317531 1215 2 -0.06907531288302238 1215 3 -1.5719970945582418E-4 1215 4 -0.0023038942147408648 1215 5 -0.035922598128102834 1215 6 0.004231139381829507 1215 7 0.012870526665565762 1215 8 0.017698002960486292 1215 9 0.012282973623648274 1215 10-11 -0.015911891149599455 1215 12-13 0.013486677005282388 1215 14-15 0.016884284172924424 1215 16-17 -0.008859722567734707 1215 18-19 0.032100473473562374 1215 20-21 -0.0033758506703591706 1215 22-23 0.023704367581970587 1215 24-25 -0.012568062271576252 1215 26-27 0.021457343383502803 1215 28-29 0.018902970001924757 1215 30-31 0.051536986630779325 1215 32-33 0.0165396293676352 1215 34-35 0.013494848885166277 1215 36-37 0.08719073529805144 1215 38-39 0.06337502727470223 1215 40-41 0.03418024322207458 1215 42-43 0.07481007205342394 1215 44-45 0.0975320590608959 1215 46-47 0.08783359816222003 1215 48-49 0.0884223332627343 1215 50-51 0.1195963095643151 1215 52-53 0.11210104387635766 1215 54-55 0.12698647707000532 1215 56-57 0.14698455735628357 1215 58-59 0.11369083653521983 1215 60-61 0.1608070785727449 1215 62-63 0.14404181421438977 1215 64-65 0.19590128169303966 1215 66-67 0.25790277168895415 1215 68-69 0.2561240355187593 1215 70-71 0.22104429353849042 1215 72-73 0.2856829801349008 1215 74-75 0.2623914556862488 1215 76-77 0.29922735431797776 1215 78-79 0.25606943546906535 1215 80-81 0.29086666487491897 1215 82-83 0.33760629688137556 1215 84-85 0.3225022734226357 1215 86-87 0.3683767454540856 1215 88-89 0.3930505407252838 1215 90-91 0.38620591631944023 1215 92-93 0.45657207210158646 1215 94-95 0.43259900015689823 1215 96-97 0.4584841476313315 1215 98-99 0.4194696750031852 1215 100-101 0.5014538346563668 1212 1 -0.15356844168207573 1212 2 -0.10353129470959743 1212 3 -0.11964940048677164 1212 4 -0.049679349160115294 1212 5 -0.001581161347246507 1212 6 -0.10721502240383529 1212 7 -0.12161829715133621 1212 8 -0.2222639732720566 1212 9 -0.14465314720877842 1212 10-11 -0.23793510300254184 1212 12-13 -0.23922505408994965 1212 14-15 -0.11452025071469052 1212 16-17 -0.01681371604302484 1212 18-19 -0.03708985901274531 1212 20-21 -0.034110309791230975 1212 22-23 4.423536674380557E-5 1212 24-25 0.0031791473992583974 1212 26-27 -0.023165300883491113 1212 28-29 0.031252220673330555 1212 30-31 -0.03056140982582889 1212 32-33 -0.06520615909870031 1212 34-35 -0.034891710537898746 1212 36-37 -0.027195718064646712 1212 38-39 -0.08706228883885103 1212 40-41 -0.12065667079180287 1212 42-43 -0.18615539517453783 1212 44-45 0.03290782773190415 1212 46-47 -0.03915313628320405 1212 48-49 6.74033027770804E-4 1212 50-51 0.02923921877233937 1212 52-53 -0.03857803379688818 1212 54-55 -0.06696172567998815 1212 56-57 -0.08015527644094078 1212 58-59 0.05596254155496183 1212 60-61 -0.26887005039390033 1212 62-63 -0.20671816267338272 1212 64-65 -0.12320912618796598 1212 66-67 -0.09482312070331744 1212 68-69 0.06564705913943669 1212 70-71 0.012895908646854082 1212 72-73 0.1357635020196426 1212 74-75 0.06390637092974316 1212 76-77 0.053411482156491275 1212 78-79 0.20670370206540412 1212 80-81 0.19595939764514725 1212 82-83 0.15990367602810807 1212 84-85 0.20387446387064045 1212 86-87 0.22229853373475095 1212 88-89 0.21044537861811108 1212 90-91 0.18968198103612366 1212 92-93 0.31112815876427646 1212 94-95 0.28259212233565734 1212 96-97 0.30133805183547935 1212 98-99 0.24947602370660604 1212 100-101 0.3195284809289092 1213 1 -0.33433467586387167 1213 2 -0.23272226780999006 1213 3 -0.2617202122030733 1213 4 -0.2498236599023187 1213 5 -0.23321183038144966 1213 6 -0.15793171694399888 1213 7 -0.11678947058967992 1213 8 -0.107351911315277 1213 9 -0.13247052308054919 1213 10-11 -0.14634135136586224 1213 12-13 -0.2121623601529734 1213 14-15 -0.24168468821252276 1213 16-17 -0.38689066764295177 1213 18-19 -0.4399407716445154 1213 20-21 -0.2848875427611617 1213 22-23 -0.34370127840317366 1213 24-25 -0.3943431741611718 1213 26-27 -0.1976474936759942 1213 28-29 -0.24543306767201756 1213 30-31 -0.23819970522711742 1213 32-33 -0.22089348535197928 1213 34-35 -0.2435046113485484 1213 36-37 -0.1528226526068721 1213 38-39 -0.07138460568422289 1213 40-41 -0.04680160708884529 1213 42-43 -0.019626872437356724 1213 44-45 -0.01391811228899087 1213 46-47 -0.014826932416553973 1213 48-49 -0.02238124984175016 1213 50-51 -0.017230503835740762 1213 52-53 0.00956392224723146 1213 54-55 0.010668533799908175 1213 56-57 0.03511594764173509 1213 58-59 0.03128271978866337 1213 60-61 0.07583826627718793 1213 62-63 0.15578935442739805 1213 64-65 0.09850763046890165 1213 66-67 0.1383927063993653 1213 68-69 0.13580303313583642 1213 70-71 0.060579946473048096 1213 72-73 0.07013939152823667 1213 74-75 0.1264482245591836 1213 76-77 0.16046210289246687 1213 78-79 0.22041445004966675 1213 80-81 0.15286856465019838 1213 82-83 0.17823780492460628 1213 84-85 0.19022765847807932 1213 86-87 0.2193461701735373 1213 88-89 0.20628534625161166 1213 90-91 0.2498765023169014 1213 92-93 0.24047826237270442 1213 94-95 0.19071130804631764 1213 96-97 0.23355830184465276 1213 98-99 0.27172086738826096 1213 100-101 0.023653575032462015 >>END_MODULE >>Per sequence quality scores pass #Quality Count 13 23.0 14 40.0 15 88.0 16 144.0 17 253.0 18 538.0 19 1288.0 20 2740.0 21 5869.0 22 11078.0 23 18972.0 24 28589.0 25 40413.0 26 55721.0 27 72572.0 28 93699.0 29 116175.0 30 141922.0 31 172646.0 32 207805.0 33 253713.0 34 315570.0 35 409130.0 36 572637.0 37 924594.0 38 2064909.0 39 7714437.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 17.7853149414432 30.51833891809918 30.49490257686242 21.201443563595195 2 19.97976944302757 31.20553583529078 30.387898019725174 18.426796701956473 3 20.03245986088307 30.254140371318726 30.119318153893616 19.594081613904585 4 20.04768038265284 29.58640330299688 30.260249751144848 20.10566656320543 5 20.480398050969352 29.834027221129656 30.227607950584794 19.457966777316194 6 20.32372908076139 30.08759928214787 29.41428967306879 20.174381964021954 7 20.51579648960177 30.24625412978576 29.72607975538285 19.51186962522962 8 19.94378001907974 30.259837267978405 29.82781732959703 19.968565383344824 9 19.824323737807482 29.93424937781292 30.331417736637896 19.9100091477417 10-11 19.93335256376571 30.17127434631337 29.982533827477315 19.912839262443608 12-13 19.72733315093391 30.246287990895805 30.16866931126928 19.857709546901006 14-15 19.722187283479826 30.129053065818884 30.276495188831205 19.87226446187009 16-17 19.787595340440028 30.023355113948046 30.30011109708924 19.888938448522683 18-19 19.76374922356814 29.91787496413197 30.29911387528624 20.019261937013656 20-21 19.836567124003597 29.87307070434565 30.242238660435493 20.04812351121526 22-23 19.90325158745881 29.83833552354006 30.181920738208046 20.076492150793086 24-25 19.93459331531494 29.818360425507894 30.14211610480449 20.104930154372674 26-27 20.02020170858198 29.79535760699015 30.0620206139544 20.122420070473467 28-29 20.028547464838812 29.871112978737603 30.003215417672546 20.097124138751038 30-31 20.111132198735145 29.741853455967526 30.041209082028004 20.105805263269325 32-33 20.141021212311504 29.806051315697296 29.947123188969883 20.105804283021314 34-35 20.153280217988314 29.77516174188912 29.949582338022378 20.12197570210018 36-37 20.166814067348398 29.78195613004854 29.927282414362672 20.12394738824039 38-39 20.174759003130365 29.735061766997195 29.93517901152766 20.155000218344778 40-41 20.222237959306348 29.741440506052196 29.89412971179421 20.14219182284725 42-43 20.208961553778444 29.715334369189662 29.913346226835596 20.1623578501963 44-45 20.205107244883045 29.64613546182781 29.914801111534793 20.23395618175435 46-47 20.223880210441482 29.730325199252693 29.873507261400317 20.172287328905508 48-49 20.2181599646904 29.663111016028576 29.875061627509215 20.243667391771808 50-51 20.17483496312088 29.696232928973004 29.88316179943548 20.245770308470632 52-53 20.226977864398606 29.68554844123344 29.904327206519593 20.183146487848365 54-55 20.228469309919447 29.64503181490571 29.8977006137328 20.228798261442048 56-57 20.242575013807162 29.668161927351587 29.88503087408757 20.20423218475368 58-59 20.217839591155855 29.657533783031443 29.91703316756401 20.20759345824869 60-61 20.22325085925698 29.65303405644039 29.839068877685566 20.28464620661706 62-63 20.207946386525492 29.628840319471756 29.92137739907021 20.241835894932546 64-65 20.24601900265781 29.637827263763434 29.8693181180203 20.246835615558457 66-67 20.241169886496944 29.667126971876517 29.825357990449053 20.266345151177482 68-69 20.18467816904681 29.626076672712255 29.86226022597806 20.32698493226288 70-71 20.273783855730258 29.64965425314623 29.810951996955144 20.26560989416837 72-73 20.27667310253676 29.673169453674404 29.812235663995406 20.237921779793435 74-75 20.261591486100723 29.65577255480316 29.832403679581287 20.250232279514833 76-77 20.247126223345756 29.68672909202068 29.816030794000188 20.250113890633376 78-79 20.25140550611438 29.676156369228053 29.838425937534495 20.234012187123067 80-81 20.271841436731926 29.668943892217882 29.81386271453454 20.245351956515655 82-83 20.25327376406901 29.720458359875423 29.846339922277345 20.179927953778222 84-85 20.268352326245765 29.68085327630103 29.795994302315336 20.254800095137874 86-87 20.205501699008956 29.68414382585523 29.848580879639925 20.26177359549589 88-89 20.26811784359263 29.64908128007478 29.80696936995256 20.275831506380033 90-91 20.260447716276172 29.6477664243644 29.78843391554234 20.303351943817088 92-93 20.207269735837095 29.67788290946733 29.818427515751857 20.296419838943724 94-95 20.255212801425657 29.668205510198533 29.76900104089082 20.307580647484986 96-97 20.280519373275634 29.700505512764973 29.735234300593405 20.283740813365984 98-99 20.271317370791095 29.686605332880962 29.750011446068076 20.29206585025987 100-101 20.324834724346 29.7135095978759 29.644476585919087 20.317179091859007 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1401.0 1 1357.0 2 1507.0 3 1988.0 4 2545.0 5 3172.5 6 3996.0 7 4946.5 8 6074.5 9 7280.5 10 8537.5 11 10071.5 12 11806.5 13 13649.0 14 15613.5 15 18048.5 16 21244.0 17 25843.0 18 32406.0 19 41471.0 20 53733.5 21 69279.5 22 88115.0 23 109999.0 24 134022.5 25 162197.0 26 194061.0 27 227160.5 28 261638.0 29 296494.0 30 330857.0 31 364306.5 32 396036.0 33 426412.5 34 454766.5 35 480241.5 36 504605.0 37 523781.5 38 532616.5 39 529899.0 40 517911.0 41 498681.5 42 471849.0 43 441736.5 44 413564.5 45 389362.5 46 369970.5 47 355401.5 48 339932.5 49 319926.0 50 298760.5 51 276517.0 52 252379.0 53 230041.5 54 211083.5 55 193661.0 56 178104.5 57 162806.5 58 146222.5 59 128540.5 60 110179.5 61 92662.0 62 77156.5 63 64153.5 64 53513.5 65 44719.5 66 37133.5 67 30814.0 68 25339.5 69 20655.0 70 16889.5 71 13683.5 72 11167.5 73 9342.0 74 7909.0 75 6745.0 76 5733.5 77 5022.0 78 4464.0 79 3826.0 80 3211.0 81 2665.0 82 2263.0 83 1932.0 84 1570.5 85 1214.0 86 911.0 87 645.0 88 419.5 89 277.5 90 173.5 91 109.0 92 56.5 93 22.5 94 19.5 95 15.5 96 8.0 97 2.0 98 3.0 99 3.0 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.08351250022210771 2 2.041500684469813E-4 3 0.0 4 0.0 5 0.0024498008213637754 6 0.0 7 0.0 8 1.3610004563132085E-4 9 0.00334957334525973 10-11 0.0 12-13 8.695280693112166E-5 14-15 2.7598064808573395E-4 16-17 0.0014819782746521605 18-19 0.0 20-21 9.829447740039839E-5 22-23 1.890278411546123E-4 24-25 4.15861250540147E-5 26-27 0.0033722566861982836 28-29 0.0016823477862760495 30-31 0.00245736193500996 32-33 0.0029601759924812285 34-35 0.004808868278973337 36-37 0.006358896576441157 38-39 0.008071488817301945 40-41 0.009954206115201883 42-43 0.017897156000518694 44-45 0.011398378821623121 46-47 0.013923790779448744 48-49 0.014861368871575619 50-51 0.011727287265232146 52-53 0.016339566589404688 54-55 0.013095848835191541 56-57 0.01095983423014442 58-59 0.008041244362717206 60-61 0.004699232131103661 62-63 0.0017617394795609866 64-65 0.0015462477406447286 66-67 0.002132234048224027 68-69 0.009954206115201883 70-71 0.00486557663131972 72-73 0.0015878338656987432 74-75 0.021250509902601515 76-77 0.03438794486284707 78-79 0.0375900764920062 80-81 0.025216314010025283 82-83 0.04533643742252221 84-85 0.0200369511623889 86-87 0.017148605749546426 88-89 0.01720153354506972 90-91 0.005754007484746399 92-93 0.017851789318641585 94-95 0.020842209765707553 96-97 0.012593034777720271 98-99 0.0037767762662691538 100-101 6.426946599256817E-5 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1.3225565E7 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 95.72517363952535 #Duplication Level Percentage of deduplicated Percentage of total 1 98.3691775986564 94.16406606408692 2 1.1230668417837364 2.1501153687708308 3 0.16546298402494744 0.4751691863010631 4 0.10453454681140799 0.40026350579404485 5 0.039212311018501395 0.18768026405265595 6 0.032686807810354455 0.1877370212020784 7 0.016815441478387413 0.11267627387407386 8 0.01635328885398494 0.12523371321000182 9 0.017448781676724708 0.15032588902223767 >10 0.11410489569700358 1.9615012849522162 >50 0.0011252877807441043 0.0636218510309008 >100 0.0 0.0 >500 0.0 0.0 >1k 1.1214407736267419E-5 0.021609577702989123 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 6.805002281566043E-5 0.0 2.2683340938553474E-5 2 6.805002281566043E-5 0.0 4.536668187710695E-5 3 9.829447740039839E-5 0.0 6.048890916947594E-5 4 1.1341670469276738E-4 0.0 7.561113646184492E-5 5 1.2097781833895188E-4 7.561113646184492E-6 8.317225010802942E-5 6 1.2097781833895188E-4 7.561113646184492E-6 9.829447740039839E-5 7 1.4366115927750533E-4 2.2683340938553474E-5 1.0585559104658289E-4 8 1.5878338656987432E-4 2.2683340938553474E-5 1.2097781833895188E-4 9 1.5878338656987432E-4 2.2683340938553474E-5 1.2097781833895188E-4 10-11 1.8524728433152006E-4 2.2683340938553474E-5 1.3610004563132085E-4 12-13 2.2683340938553476E-4 2.2683340938553474E-5 1.398806024544131E-4 14-15 2.4951675032408826E-4 2.2683340938553474E-5 1.5122227292368984E-4 16-17 2.948834322011952E-4 2.2683340938553474E-5 1.6256394339296656E-4 18-19 3.440306709013944E-4 2.2683340938553474E-5 1.7390561386224331E-4 20-21 4.196418073632393E-4 2.2683340938553474E-5 1.9280839797770454E-4 22-23 4.952529438250842E-4 2.2683340938553474E-5 2.0415006844698127E-4 24-25 5.519612961714679E-4 3.024445458473797E-5 2.1927229573935026E-4 26-27 6.048890916947594E-4 3.024445458473797E-5 2.30613966208627E-4 28-29 7.22086353210619E-4 3.780556823092246E-5 2.45736193500996E-4 30-31 8.241613874341097E-4 3.780556823092246E-5 2.7976120490882624E-4 32-33 9.22455864834508E-4 4.15861250540147E-5 3.289084436090254E-4 34-35 0.0010509947968196445 5.2927795523291444E-5 3.553723413706711E-4 36-37 0.0011568503878662273 5.2927795523291444E-5 3.6293345501685564E-4 38-39 0.0012475837516204413 5.2927795523291444E-5 3.780556823092246E-4 40-41 0.0013156337744361017 5.2927795523291444E-5 3.8939735277850133E-4 42-43 0.0014101476950134078 5.670835234638369E-5 4.0451958007087035E-4 44-45 0.001538686626998544 6.048890916947594E-5 4.2342236418633155E-4 46-47 0.001739056138622433 6.048890916947594E-5 4.4232514830179275E-4 48-49 0.0018864978547230306 6.048890916947594E-5 4.876918301788997E-4 50-51 0.0020793062527007353 6.426946599256819E-5 5.2171684158673E-4 52-53 0.0022872368779708087 8.317225010802942E-5 5.368390688790989E-4 54-55 0.0024687036054792367 8.317225010802942E-5 5.557418529945602E-4 56-57 0.0027635870376804318 8.695280693112166E-5 5.670835234638369E-4 58-59 0.00316432606092821 9.07333637542139E-5 5.822057507562058E-4 60-61 0.003565065084175988 9.07333637542139E-5 5.935474212254827E-4 62-63 0.0040830013689396255 9.829447740039839E-5 6.237918758102206E-4 64-65 0.004782404381211691 9.829447740039839E-5 6.615974440411431E-4 66-67 0.005746446371100214 9.829447740039839E-5 7.107446827413422E-4 68-69 0.0071187884978826995 9.829447740039839E-5 7.22086353210619E-4 70-71 0.009111141943652312 1.0207503422349063E-4 7.409891373260803E-4 72-73 0.011553381651369904 1.0585559104658289E-4 7.598919214415415E-4 74-75 0.015054177269553324 1.0963614786967514E-4 7.787947055570027E-4 76-77 0.019991584480511797 1.1341670469276738E-4 7.863558192031871E-4 78-79 0.02693646736453225 1.1719726151585962E-4 7.976974896724638E-4 80-81 0.037147751343704405 1.2097781833895188E-4 8.27941944257202E-4 82-83 0.051086664350445524 1.2097781833895188E-4 8.619669556650321E-4 84-85 0.06915016485118028 1.2475837516204413E-4 8.808697397804932E-4 86-87 0.09294498949572287 1.3610004563132085E-4 8.997725238959546E-4 88-89 0.12487557242355998 1.3610004563132085E-4 9.186753080114157E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GATCGGA 4615 0.0 10.653604 94-95 AGATCGG 5080 0.0 9.865442 94-95 ATCGGAA 4635 0.0 9.787719 94-95 TATGCCG 985 0.0 8.921854 46-47 TCGGAAG 4330 0.0 8.557256 94-95 TAGATCG 2190 0.0 8.24336 92-93 CTCGTAT 1130 0.0 7.146432 42-43 TCTCGTA 1115 0.0 7.029848 40-41 ATGCCGT 1555 0.0 6.7206593 46-47 CGGAAGA 4375 0.0 6.351929 94-95 GCCGTCT 1725 0.0 6.0581965 48-49 TCGTATG 1945 0.0 5.983638 42-43 CGTATGC 1510 0.0 5.9767385 44-45 AAGATCG 3230 0.0 5.8092813 94-95 CAGATCG 1815 0.0 5.627165 94-95 TGCCGTC 1715 0.0 5.5395646 48-49 CGTCTGA 1725 0.0 5.368456 16-17 ACACGTC 1915 0.0 5.333318 94-95 >>END_MODULE