FastQCFastQC Report
Fri 3 Apr 2015
H2K5YBCXX l01n01 50072-bar8.341000000044d7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2K5YBCXX l01n01 50072-bar8.341000000044d7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13225565
Sequences flagged as poor quality0
Sequence length101
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA46150.010.65360494-95
AGATCGG50800.09.86544294-95
ATCGGAA46350.09.78771994-95
TATGCCG9850.08.92185446-47
TCGGAAG43300.08.55725694-95
TAGATCG21900.08.2433692-93
CTCGTAT11300.07.14643242-43
TCTCGTA11150.07.02984840-41
ATGCCGT15550.06.720659346-47
CGGAAGA43750.06.35192994-95
GCCGTCT17250.06.058196548-49
TCGTATG19450.05.98363842-43
CGTATGC15100.05.976738544-45
AAGATCG32300.05.809281394-95
CAGATCG18150.05.62716594-95
TGCCGTC17150.05.539564648-49
CGTCTGA17250.05.36845616-17
ACACGTC19150.05.33331894-95