FastQCFastQC Report
Fri 3 Apr 2015
H2K5YBCXX l01n01 12330-bar9.34100000004487.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2K5YBCXX l01n01 12330-bar9.34100000004487.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14900464
Sequences flagged as poor quality0
Sequence length101
%GC25

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGC238730.16021648721811615TruSeq Adapter, Index 8 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTCT38200.034.133948-49
TATGCCG39000.033.19162446-47
ATGCCGT41100.032.18918246-47
TGCCGTC40750.031.06536948-49
AGAGCAC92500.029.1112468
CGTCTGA47300.027.81186316-17
AATCTCG42600.027.71057538-39
GAGCACA97750.027.6448049
CGTATGC46900.027.44762644-45
CTCGTAT45300.027.10819642-43
ACGTCTG49000.027.04111914-15
TCTCGTA44550.026.92625840-41
ATCTCGT45850.025.59292440-41
CAGTCAC53400.025.12614326-27
CACGTCT53600.024.67612514-15
ACACGTC54200.024.44676812-13
CCGTCTT52850.024.17741250-51
GTATGCC56100.024.089544-45
CGGAAGA111450.024.0338234
TCGGAAG116200.023.1739863
Produced by FastQC (version 0.11.2)