##FastQC	0.11.2
>>Basic Statistics	pass
#Measure	Value
Filename	H2K5YBCXX l01n01 12330-bar9.34100000004487.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	14900464
Sequences flagged as poor quality	0
Sequence length	101
%GC	25
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	37.09692235087444	38.0	38.0	38.0	32.0	38.0
2	37.08700393491102	38.0	38.0	38.0	32.0	38.0
3	37.05898588124504	38.0	38.0	38.0	32.0	38.0
4	37.03140472672529	38.0	38.0	38.0	32.0	38.0
5	37.00381592143708	38.0	38.0	38.0	32.0	38.0
6	38.876361702561745	40.0	38.0	40.0	38.0	40.0
7	38.82306960373852	40.0	38.0	40.0	38.0	40.0
8	38.80136712521167	40.0	38.0	40.0	38.0	40.0
9	38.81026564004987	40.0	38.0	40.0	38.0	40.0
10-11	38.80318857855702	40.0	38.0	40.0	38.0	40.0
12-13	38.78227027024125	40.0	38.0	40.0	38.0	40.0
14-15	38.75389897254206	40.0	38.0	40.0	38.0	40.0
16-17	38.724759208840744	40.0	38.0	40.0	38.0	40.0
18-19	38.66748344212637	40.0	38.0	40.0	38.0	40.0
20-21	38.625256904751424	40.0	38.0	40.0	38.0	40.0
22-23	38.58236183114835	40.0	38.0	40.0	38.0	40.0
24-25	38.51171983637556	40.0	38.0	40.0	38.0	40.0
26-27	38.39951973978797	40.0	38.0	40.0	38.0	40.0
28-29	38.31509079180353	40.0	38.0	40.0	38.0	40.0
30-31	38.26167940139314	40.0	38.0	40.0	38.0	40.0
32-33	38.18562301147132	40.0	38.0	40.0	38.0	40.0
34-35	38.13648497791747	40.0	38.0	40.0	38.0	40.0
36-37	38.08016750350862	40.0	38.0	40.0	38.0	40.0
38-39	38.01994162732113	40.0	38.0	40.0	38.0	40.0
40-41	37.948772568424715	40.0	38.0	40.0	38.0	40.0
42-43	37.86327422421208	40.0	38.0	40.0	32.0	40.0
44-45	37.82299903546628	40.0	38.0	40.0	32.0	40.0
46-47	37.762926107535975	40.0	38.0	40.0	32.0	40.0
48-49	37.672961962795256	40.0	38.0	40.0	32.0	40.0
50-51	37.628348956113044	40.0	38.0	40.0	32.0	40.0
52-53	37.57527913224716	40.0	38.0	40.0	32.0	40.0
54-55	37.482614434020306	40.0	38.0	40.0	32.0	40.0
56-57	37.44802893386407	40.0	38.0	40.0	32.0	40.0
58-59	37.3931203081998	40.0	38.0	40.0	32.0	40.0
60-61	37.307994704057535	40.0	38.0	40.0	32.0	40.0
62-63	37.25774472526493	40.0	38.0	40.0	32.0	40.0
64-65	37.169998464477345	40.0	38.0	40.0	32.0	40.0
66-67	37.09535605736842	40.0	38.0	40.0	32.0	40.0
68-69	37.02740780421335	40.0	38.0	40.0	32.0	40.0
70-71	36.9830274412931	40.0	38.0	40.0	32.0	40.0
72-73	36.929085396266856	40.0	38.0	40.0	32.0	40.0
74-75	36.803671717874025	40.0	38.0	40.0	32.0	40.0
76-77	36.717836605625166	40.0	38.0	40.0	32.0	40.0
78-79	36.6198560662272	38.0	38.0	40.0	29.5	40.0
80-81	36.55973085804577	38.0	38.0	40.0	27.0	40.0
82-83	36.46428651483605	38.0	38.0	40.0	27.0	40.0
84-85	36.408169168423214	38.0	38.0	40.0	27.0	40.0
86-87	36.345390083154456	38.0	38.0	40.0	27.0	40.0
88-89	36.254018666801244	38.0	38.0	40.0	27.0	40.0
90-91	36.180368879787906	38.0	38.0	40.0	27.0	40.0
92-93	36.079130086150336	38.0	38.0	40.0	27.0	40.0
94-95	35.983481554668366	38.0	38.0	40.0	27.0	40.0
96-97	35.88084132816267	38.0	38.0	40.0	27.0	40.0
98-99	35.83509946401669	38.0	38.0	40.0	27.0	40.0
100-101	34.96858664938219	38.0	35.0	40.0	20.0	40.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
2201	1	0.10190733667025853
2201	2	0.10965290659926552
2201	3	0.05301156152371789
2201	4	0.06069538557583343
2201	5	0.020394369842620108
2201	6	0.06339731069788712
2201	7	0.03923233334963072
2201	8	0.02640458061146944
2201	9	-0.0033821994375742293
2201	10-11	-4.6404919766018793E-4
2201	12-13	-0.028858158891573282
2201	14-15	-0.0279410345147042
2201	16-17	-0.31661677224506946
2201	18-19	-0.31675374418895785
2201	20-21	-0.27154824675084654
2201	22-23	-0.3008377042690853
2201	24-25	-0.27652810807769157
2201	26-27	-0.2761700265726219
2201	28-29	-0.35706695779332875
2201	30-31	-0.33652801269228405
2201	32-33	-0.3993566396899766
2201	34-35	-0.40878490082950236
2201	36-37	-0.4297474139248365
2201	38-39	-0.3723821341705076
2201	40-41	-0.4240752543614903
2201	42-43	-0.42644719860545877
2201	44-45	-0.43698591641167894
2201	46-47	-0.39237361491694145
2201	48-49	-0.43042425317420197
2201	50-51	-0.4857721409918909
2201	52-53	-0.38137396223578435
2201	54-55	-0.43793478230572447
2201	56-57	-0.4848726727371613
2201	58-59	-0.46578059919517045
2201	60-61	-0.46625115058575517
2201	62-63	-0.43471218560911495
2201	64-65	-0.5240854916673356
2201	66-67	-0.45550109577437325
2201	68-69	-0.5351038114639408
2201	70-71	-0.5908039149099409
2201	72-73	-0.5182440712609377
2201	74-75	-0.4661983280962403
2201	76-77	-0.5697553344644959
2201	78-79	-0.5301844829490889
2201	80-81	-0.5847662385852246
2201	82-83	-0.6740424342967088
2201	84-85	-0.7095270510673544
2201	86-87	-0.6899966803204833
2201	88-89	-0.6932987728628319
2201	90-91	-0.6016130539488671
2201	92-93	-0.7595835544733021
2201	94-95	-0.7014872921388928
2201	96-97	-0.7172467679170325
2201	98-99	-0.6689694414420302
2201	100-101	-0.7056445029908573
1101	1	0.0640667549506091
1101	2	0.07798905617951135
1101	3	0.06262286870954625
1101	4	0.029742972853206595
1101	5	0.0787435524847595
1101	6	0.06504637807661595
1101	7	0.07722890641687741
1101	8	0.016064004414971578
1101	9	0.02006682532881854
1101	10-11	-0.0015762210822600764
1101	12-13	0.018852984947322682
1101	14-15	-0.013173711476881067
1101	16-17	-0.1042543732017549
1101	18-19	-0.16478415461875784
1101	20-21	-0.20064745833256126
1101	22-23	-0.221684422180509
1101	24-25	-0.1457223382547781
1101	26-27	-0.12733722515528
1101	28-29	-0.21096170242500278
1101	30-31	-0.22373795367170146
1101	32-33	-0.27196639121828525
1101	34-35	-0.23895331192282754
1101	36-37	-0.24075898343591007
1101	38-39	-0.24988533539229962
1101	40-41	-0.2692319877832787
1101	42-43	-0.2655194833047432
1101	44-45	-0.2911067331041721
1101	46-47	-0.30369950936524504
1101	48-49	-0.3402442410938349
1101	50-51	-0.39859123672491137
1101	52-53	-0.31373371784216886
1101	54-55	-0.42255993007930925
1101	56-57	-0.34720703589747615
1101	58-59	-0.48815759984836404
1101	60-61	-0.5468037723869941
1101	62-63	-0.6167200965669082
1101	64-65	-0.615317832400919
1101	66-67	-0.5673564605776917
1101	68-69	-0.627697416152877
1101	70-71	-0.6285018181390711
1101	72-73	-0.6498997466190559
1101	74-75	-0.6088537652833281
1101	76-77	-0.6531993300063093
1101	78-79	-0.6795613113199792
1101	80-81	-0.6487322583429744
1101	82-83	-0.6652005039184132
1101	84-85	-0.7459747969333534
1101	86-87	-0.708970122672298
1101	88-89	-0.8114731223938492
1101	90-91	-0.7598996481741409
1101	92-93	-0.8205383941040623
1101	94-95	-0.7724079159280777
1101	96-97	-0.7896242134869453
1101	98-99	-0.8085507798954978
1101	100-101	-0.8050107608919106
2202	1	0.034352495878287925
2202	2	0.05833985704437339
2202	3	0.06897113043756065
2202	4	0.04648743624353102
2202	5	0.041577658632213854
2202	6	-0.030599975802012125
2202	7	-0.03693261004717385
2202	8	-0.08005021809476176
2202	9	-0.07870971314487463
2202	10-11	-0.01428089873181193
2202	12-13	-0.03654445499662984
2202	14-15	-0.1006319103460811
2202	16-17	-0.07807206636618247
2202	18-19	-0.06958427534891598
2202	20-21	-0.10116170869365959
2202	22-23	-0.13861950916849253
2202	24-25	-0.0965197419428776
2202	26-27	-0.15401926283254141
2202	28-29	-0.16540879602123226
2202	30-31	-0.1426741774126583
2202	32-33	-0.18536507711220906
2202	34-35	-0.2447149305903622
2202	36-37	-0.14018523052846632
2202	38-39	-0.2352914405471651
2202	40-41	-0.2806897977148637
2202	42-43	-0.25758611064822645
2202	44-45	-0.2667955547980583
2202	46-47	-0.26690113333312837
2202	48-49	-0.27913304634682845
2202	50-51	-0.3935157665720368
2202	52-53	-0.35590413202083937
2202	54-55	-0.2995252020611048
2202	56-57	-0.3420883090117357
2202	58-59	-0.3839018617150174
2202	60-61	-0.3732103686215993
2202	62-63	-0.3633315058737381
2202	64-65	-0.37580210389286606
2202	66-67	-0.32200624956062995
2202	68-69	-0.3644344271550892
2202	70-71	-0.4541439568910306
2202	72-73	-0.41562870188909073
2202	74-75	-0.40148488096681234
2202	76-77	-0.42486738095959
2202	78-79	-0.5049464506519641
2202	80-81	-0.5356891105003712
2202	82-83	-0.6080585916888666
2202	84-85	-0.5814231153149763
2202	86-87	-0.5981557676845242
2202	88-89	-0.6670287446127432
2202	90-91	-0.6306456973747459
2202	92-93	-0.666620229889574
2202	94-95	-0.643591159219902
2202	96-97	-0.6289625312336398
2202	98-99	-0.6531139848640848
2202	100-101	-0.6855749277755976
1102	1	0.053859356154653426
1102	2	0.04834229745771523
1102	3	0.00425787245598741
1102	4	0.017752262766698834
1102	5	-0.03301270646826282
1102	6	-0.1736491183679334
1102	7	-0.17597557150275378
1102	8	-0.14860848154926032
1102	9	-0.114759499005892
1102	10-11	-0.13684934000189486
1102	12-13	-0.10716296909509992
1102	14-15	-0.1034556644567175
1102	16-17	-0.12285477997602356
1102	18-19	-0.11106014374520612
1102	20-21	-0.16951749583413545
1102	22-23	-0.2819274105648404
1102	24-25	-0.1732447405809694
1102	26-27	-0.2217979395498091
1102	28-29	-0.2640869005005797
1102	30-31	-0.2797436678699512
1102	32-33	-0.22445235418783227
1102	34-35	-0.2674291286579802
1102	36-37	-0.3083548794429376
1102	38-39	-0.3126457812411303
1102	40-41	-0.320272762689072
1102	42-43	-0.349595644871016
1102	44-45	-0.39715317031358666
1102	46-47	-0.4515833083433307
1102	48-49	-0.3859450990952382
1102	50-51	-0.41991415645888
1102	52-53	-0.37108342083593016
1102	54-55	-0.49331620230014295
1102	56-57	-0.48762388448103167
1102	58-59	-0.4591866783134009
1102	60-61	-0.3956981101437833
1102	62-63	-0.5314685643451256
1102	64-65	-0.4609848071433049
1102	66-67	-0.48807584748184496
1102	68-69	-0.48821914090365937
1102	70-71	-0.5895176059638843
1102	72-73	-0.5921030125050635
1102	74-75	-0.5933567018134411
1102	76-77	-0.6330025330627151
1102	78-79	-0.7493134426177761
1102	80-81	-0.667422042714449
1102	82-83	-0.7375507791214275
1102	84-85	-0.7465364081851149
1102	86-87	-0.7334237413228166
1102	88-89	-0.7336252801069421
1102	90-91	-0.7424926663712554
1102	92-93	-0.9846737326328778
1102	94-95	-0.932143696425193
1102	96-97	-0.8813365306805423
1102	98-99	-0.8225046263343785
1102	100-101	-0.9102203507205431
2203	1	0.04225510611952643
2203	2	0.02674780378463737
2203	3	-0.003521017670585991
2203	4	0.03551794431089661
2203	5	0.018616572141134213
2203	6	-0.18577208237862664
2203	7	-0.1707367452981572
2203	8	-0.19301625256500188
2203	9	-0.15003255668464277
2203	10-11	-0.13161036620738287
2203	12-13	-0.15106974960841058
2203	14-15	-0.12316742047923412
2203	16-17	-0.14374347331545323
2203	18-19	-0.13819836708859157
2203	20-21	-0.15644587344008443
2203	22-23	-0.2394318571318479
2203	24-25	-0.1844007047024121
2203	26-27	-0.2178286667920304
2203	28-29	-0.2524542751515284
2203	30-31	-0.30604207001381667
2203	32-33	-0.3040777686350111
2203	34-35	-0.2695211718668844
2203	36-37	-0.2976028250991547
2203	38-39	-0.3015238054625229
2203	40-41	-0.3429814299152767
2203	42-43	-0.30787183909601623
2203	44-45	-0.36139833995342485
2203	46-47	-0.3280391055597178
2203	48-49	-0.33193550534959826
2203	50-51	-0.37338526471516076
2203	52-53	-0.38558504305547814
2203	54-55	-0.41333425904975485
2203	56-57	-0.4281352538751477
2203	58-59	-0.4539780757557921
2203	60-61	-0.4006006469651098
2203	62-63	-0.47896126757474633
2203	64-65	-0.503070558732567
2203	66-67	-0.5018238536948942
2203	68-69	-0.5424090376365811
2203	70-71	-0.5390528152315852
2203	72-73	-0.530989501123301
2203	74-75	-0.5159028677020245
2203	76-77	-0.5485761178895032
2203	78-79	-0.5061378808803525
2203	80-81	-0.6543391746608194
2203	82-83	-0.6181618193049516
2203	84-85	-0.6601534131296773
2203	86-87	-0.710017202108638
2203	88-89	-0.7019278463664591
2203	90-91	-0.7216877464504137
2203	92-93	-0.8752195272590342
2203	94-95	-0.8861353197152013
2203	96-97	-0.8621373325199357
2203	98-99	-0.8522616343698814
2203	100-101	-0.9750374361181997
1103	1	0.08924519633237793
1103	2	0.033770161793228226
1103	3	0.04454515874733289
1103	4	-0.006168167783485501
1103	5	0.0647843434384967
1103	6	-0.1271650798524675
1103	7	-0.08685944795372791
1103	8	-0.08058426601654389
1103	9	-0.10003841837318106
1103	10-11	-0.02232593859132237
1103	12-13	-0.07342703989684907
1103	14-15	-0.10215549984258132
1103	16-17	-0.049767250858678835
1103	18-19	-0.014212668829728159
1103	20-21	-0.049286936403767356
1103	22-23	-0.14821288514139752
1103	24-25	-0.15138480142570643
1103	26-27	-0.15396141415575926
1103	28-29	-0.136295878355007
1103	30-31	-0.17367396897847698
1103	32-33	-0.11658145721482782
1103	34-35	-0.159984273310954
1103	36-37	-0.21650329883500063
1103	38-39	-0.1770852545142958
1103	40-41	-0.11486661655731467
1103	42-43	-0.11645824333663768
1103	44-45	-0.12477482435203768
1103	46-47	-0.14959456844711383
1103	48-49	-0.25004256275126835
1103	50-51	-0.23943585663368339
1103	52-53	-0.19997640276159956
1103	54-55	-0.17377922967045123
1103	56-57	-0.20221054182199083
1103	58-59	-0.24269007394282482
1103	60-61	-0.21220733813541415
1103	62-63	-0.22183095372085404
1103	64-65	-0.361211330240522
1103	66-67	-0.30730428502607054
1103	68-69	-0.48785269468856285
1103	70-71	-0.4017086819188975
1103	72-73	-0.389743696553964
1103	74-75	-0.35440397856541495
1103	76-77	-0.37126395043794247
1103	78-79	-0.42676035225182574
1103	80-81	-0.48959169773911526
1103	82-83	-0.5255275386669425
1103	84-85	-0.6219005787217782
1103	86-87	-0.507385340710762
1103	88-89	-0.5718765486823472
1103	90-91	-0.5742532231480055
1103	92-93	-0.5812251928826129
1103	94-95	-0.48671775333188805
1103	96-97	-0.5518883100234007
1103	98-99	-0.5089403518449487
1103	100-101	-0.4835132504622166
2204	1	0.020266495223218328
2204	2	0.07044903243512124
2204	3	0.07820662553555024
2204	4	0.04498413597145401
2204	5	0.06317400743651547
2204	6	-0.05264311090120799
2204	7	-0.01121980654888688
2204	8	0.04210083997167402
2204	9	0.05059765799368421
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1213	20-21	-0.06841860437982206
1213	22-23	-0.11427586504132137
1213	24-25	-0.10325698775866954
1213	26-27	0.04420152368407315
1213	28-29	0.028500629966302427
1213	30-31	0.08632787234627415
1213	32-33	0.11109005185091547
1213	34-35	0.08605664479519248
1213	36-37	0.14316105164814275
1213	38-39	0.1990252297454731
1213	40-41	0.2364723200804164
1213	42-43	0.2925507354835162
1213	44-45	0.3254177531072173
1213	46-47	0.34779894750420937
1213	48-49	0.34009402747184225
1213	50-51	0.35844333335192147
1213	52-53	0.4156706206383518
1213	54-55	0.3917895926873669
1213	56-57	0.4427594504136394
1213	58-59	0.4871543511670211
1213	60-61	0.5015375909824584
1213	62-63	0.521074132625543
1213	64-65	0.5358451372645803
1213	66-67	0.584015724225516
1213	68-69	0.6314684855561481
1213	70-71	0.5974624203799124
1213	72-73	0.5850312026630888
1213	74-75	0.628066088429037
1213	76-77	0.7055982867353876
1213	78-79	0.7501890004990912
1213	80-81	0.7487025487355226
1213	82-83	0.7019980020250003
1213	84-85	0.7745103009737733
1213	86-87	0.813627404766649
1213	88-89	0.8371191393299213
1213	90-91	0.851659975504603
1213	92-93	0.9351962049606186
1213	94-95	0.8761483240354906
1213	96-97	0.9175735453232363
1213	98-99	0.933196393497596
1213	100-101	0.7673937358665839
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	65.0
14	107.0
15	262.0
16	584.0
17	1183.0
18	2864.0
19	6354.0
20	12415.0
21	21007.0
22	32902.0
23	48053.0
24	66291.0
25	87212.0
26	111254.0
27	137970.0
28	166310.0
29	199254.0
30	234221.0
31	273817.0
32	322355.0
33	375234.0
34	444138.0
35	552233.0
36	735097.0
37	1116698.0
38	2285475.0
39	7667109.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	11.960961465829763	35.82276238964394	38.7194059346487	13.496870209877592
2	12.589249066268934	38.977013826977945	36.69958382425856	11.734153282494553
3	12.458719406321842	37.77567597894938	37.15866163630878	12.606942978420003
4	12.779971147207227	37.54156246409508	36.68950174974417	12.988964638953526
5	13.422667267738516	36.924368503754586	37.18095155257543	12.472012675931468
6	13.261224616897834	38.183992122661415	35.569543337710826	12.985239922729924
7	13.206092105588121	37.36552767752735	36.87355642079334	12.554823796091183
8	12.904077697008379	38.52189649369532	35.85072794805792	12.723297861238372
9	12.951719535261248	37.15516039103087	37.07863637724231	12.814483696465572
10-11	12.84418391266205	37.72190584132145	36.72794686125211	12.705963384764393
12-13	12.554145572654098	37.66816808599119	36.998772487272454	12.778913854082255
14-15	12.543138472462264	37.57342705330237	37.1266442702025	12.756790204032873
16-17	12.668246037253294	37.274967962763284	37.32466179486578	12.732124205117643
18-19	12.571984335521364	37.18881170411875	37.41555967653088	12.823644283829013
20-21	12.725320497204043	37.10438589674051	37.3802199961585	12.79007360989694
22-23	12.654333177621979	37.19786407556532	37.362470024781494	12.785332722031209
24-25	12.6708512804874	36.98220303146366	37.406418151279766	12.940527536769169
26-27	12.722041341407825	36.95372219248308	37.30035809569013	13.02387837041897
28-29	12.82465636197495	37.01662634795269	37.337698454497584	12.82101883557478
30-31	12.741219896600187	36.869872253072266	37.440810473819226	12.948097376508322
32-33	12.769380040840142	37.02002664958216	37.296948613447235	12.913644696130469
34-35	12.765002199361037	36.99063852075151	37.36407687038643	12.880282409501026
36-37	12.773847662152155	36.811379674449846	37.550217548553064	12.864555114844931
38-39	12.883559049708568	36.949107619329574	37.36724462882592	12.80008870213593
40-41	12.775036228067298	36.927442415738305	37.496020290469545	12.801501065724851
42-43	12.744680289791274	36.741598080838614	37.546232630858455	12.967488998511655
44-45	12.860281832358012	36.7825464731959	37.44854387151981	12.908627822926283
46-47	12.766469282882772	36.87112235707127	37.59212322783668	12.770285132209278
48-49	12.840651811753704	36.70103790990068	37.603146703354625	12.855163574990996
50-51	12.753491818611689	36.72697483783041	37.527313523848136	12.992219819709769
52-53	12.83781035365543	36.71731125860887	37.67713111098641	12.767747276749288
54-55	12.731694582136162	36.674687709741896	37.689993290944116	12.903624417177827
56-57	12.759846678428625	36.663311381350624	37.68639150239595	12.8904504378248
58-59	12.837773645669529	36.65438231579674	37.74188245045064	12.765961588083098
60-61	12.740245480464116	36.63447934731835	37.711144340579985	12.914130831637557
62-63	12.816298367708898	36.62808964569348	37.7650179865138	12.790594000083825
64-65	12.701868068634237	36.821212222492015	37.70875723698243	12.76816247189132
66-67	12.73627149193235	36.80368877417547	37.68675513213781	12.773284601754376
68-69	12.728420827111966	36.80228360084479	37.69304243809252	12.77625313395073
70-71	12.724556323698563	36.803132597041795	37.71700919896851	12.755301880291125
72-73	12.701754984095706	36.799290990358166	37.70903256096109	12.789921464585039
74-75	12.728951744362918	36.773811988311394	37.72681376501586	12.770422502309822
76-77	12.722091957365864	36.75703883387599	37.76865699998939	12.752212208768757
78-79	12.72610557502147	36.7349846233657	37.74343456336001	12.795475238252818
80-81	12.744106237655354	36.746500334739935	37.74832363192798	12.76106979567673
82-83	12.712862526684557	36.76471955771574	37.79316252911176	12.729255386487939
84-85	12.705721148459958	36.70751582271578	37.79402340446941	12.792739624354862
86-87	12.721458100134168	36.75272784291319	37.778942897271364	12.746871159681275
88-89	12.771709736441686	36.76586983748438	37.73835847100327	12.724061955070662
90-91	12.739935204168148	36.712251988220196	37.75678887903439	12.791023928577266
92-93	12.770263399100601	36.7580071735737	37.71889820835181	12.752831218973892
94-95	12.799078596619898	36.762067668010836	37.703169280852734	12.735684454516532
96-97	12.835514014175061	36.745499128170664	37.63267894785522	12.786307909799055
98-99	12.87825747065216	36.772884576974455	37.569627965388236	12.779229986985152
100-101	12.894358462517626	36.82225581031929	37.57182039563166	12.71156533153142
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	32968.0
1	33344.0
2	39366.5
3	53624.0
4	72456.5
5	95710.0
6	123529.0
7	153839.5
8	188290.5
9	227070.0
10	267429.5
11	307407.0
12	347595.5
13	385845.0
14	419900.5
15	452125.5
16	481887.0
17	507685.0
18	526730.5
19	538707.5
20	544302.0
21	542164.5
22	533358.0
23	518459.5
24	496851.0
25	470700.5
26	441594.5
27	411986.0
28	385136.5
29	361348.0
30	341027.0
31	323804.5
32	307799.0
33	288775.5
34	266560.0
35	244792.5
36	225886.0
37	211715.0
38	201128.5
39	190652.5
40	180163.5
41	172868.0
42	164193.0
43	154136.0
44	147856.0
45	142092.0
46	135392.0
47	130709.5
48	122541.5
49	108636.0
50	95389.0
51	83476.5
52	73036.5
53	65741.0
54	60005.0
55	53976.5
56	48724.0
57	44387.5
58	40333.5
59	36103.5
60	31854.5
61	28128.5
62	24796.5
63	22202.5
64	20410.0
65	18909.5
66	17571.5
67	16434.0
68	15531.0
69	14599.0
70	13307.5
71	11976.0
72	10764.0
73	9441.0
74	8028.5
75	6650.5
76	5414.5
77	4318.0
78	3406.5
79	2616.5
80	1971.5
81	1455.0
82	1087.0
83	825.5
84	598.5
85	424.0
86	293.5
87	205.0
88	152.0
89	103.0
90	64.0
91	42.0
92	23.5
93	11.0
94	6.0
95	5.5
96	3.0
97	1.5
98	1.0
99	0.5
100	1.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.08325244099781054
2	1.879136112808299E-4
3	0.0
4	0.0
5	0.002785148167198015
6	0.0
7	0.0
8	1.2751280765484885E-4
9	0.0037985394280339187
10-11	0.0
12-13	5.0334003021650865E-5
14-15	3.120708187342354E-4
16-17	0.0015066644904480828
18-19	0.0
20-21	1.4764640886350921E-4
22-23	2.0469161228804688E-4
24-25	3.355600201443391E-5
26-27	0.003597203415947316
28-29	0.00153350929205963
30-31	0.0022851637371829494
32-33	0.0028052817684066753
34-35	0.004577038674768785
36-37	0.006150815169245736
38-39	0.007791703667751555
40-41	0.009727884983984391
42-43	0.01816721949061452
44-45	0.010784899047439058
46-47	0.013939163236795848
48-49	0.014801552488566799
50-51	0.011469441488533511
52-53	0.016509552991101482
54-55	0.013049929183413347
56-57	0.01061711903736689
58-59	0.007754792065535677
60-61	0.004724685083632295
62-63	0.0018288021097866483
64-65	0.001758334505556337
66-67	0.0022683857361757327
68-69	0.010321826219639871
70-71	0.005033400302165087
72-73	0.0016341772981029313
74-75	0.02161006529729544
76-77	0.03469690608292467
78-79	0.03774714666603671
80-81	0.025670341541041943
82-83	0.04553213913338538
84-85	0.020489294830013348
86-87	0.017529655452340275
88-89	0.017472610248915738
90-91	0.006003168760382227
92-93	0.01831486589947803
94-95	0.021398662484604508
96-97	0.012791547967902207
98-99	0.003758272225616598
100-101	9.060120543897156E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	1.4900464E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	87.28042547074662
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.01550119320086	78.56591243105072
2	8.526543403148217	14.884006720431284
3	1.0580620360449722	2.7704431404134886
4	0.17519929064673212	0.6116587451927912
5	0.06294565590550018	0.27469618144836355
6	0.038159145480690534	0.1998327871892873
7	0.020447898447007746	0.12492908935062189
8	0.005369017110234048	0.0374888078192757
9	0.009547486465527805	0.07499778127987154
>10	0.08227189593758207	1.911119484601374
>50	0.005897215196446436	0.30412454791326216
>100	0.0	0.0
>500	4.021375318663517E-5	0.031504981943942
>1k	0.0	0.0
>5k	7.857938705348444E-6	0.049037862697099
>10k+	7.69072431890818E-6	0.16024743866857546
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGC	23873	0.16021648721811615	TruSeq Adapter, Index 8 (100% over 50bp)
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA Adapter	Nextera Transposase Sequence
1	4.697840282020748E-5	0.0	0.0
2	4.697840282020748E-5	0.0	1.3422400805773565E-5
3	6.0400803625981046E-5	0.0	2.013360120866035E-5
4	6.711200402886782E-5	0.0	2.013360120866035E-5
5	8.05344048346414E-5	0.0	2.684480161154713E-5
6	8.724560523752817E-5	0.0	3.355600201443391E-5
7	8.724560523752817E-5	0.0	4.02672024173207E-5
8	1.0066800604330174E-4	6.711200402886782E-6	5.368960322309426E-5
9	1.0066800604330174E-4	6.711200402886782E-6	6.711200402886782E-5
10-11	1.6442440987072615E-4	2.3489201410103735E-5	7.046760423031121E-5
12-13	2.2482521349670724E-4	4.02672024173207E-5	7.7178804633198E-5
14-15	2.885816173241317E-4	5.368960322309426E-5	1.0066800604330174E-4
16-17	4.0938322457609376E-4	6.0400803625981046E-5	1.0066800604330174E-4
18-19	5.670964340439332E-4	6.0400803625981046E-5	1.0066800604330174E-4
20-21	8.120552487493007E-4	6.0400803625981046E-5	1.0066800604330174E-4
22-23	0.0011543264692965266	6.0400803625981046E-5	1.1073480664763191E-4
24-25	0.0016039768962899412	6.375640382742443E-5	1.2415720745340548E-4
26-27	0.002090538925499233	7.382320443175461E-5	1.3086840785629227E-4
28-29	0.002607301356521515	7.382320443175461E-5	1.3757960825917903E-4
30-31	0.0030737297845221468	7.382320443175461E-5	1.4093520846062243E-4
32-33	0.0035602918137314383	8.05344048346414E-5	1.6442440987072618E-4
34-35	0.004013297840926296	8.389000503608479E-5	1.6778001007216958E-4
36-37	0.004385769463286513	1.0066800604330174E-4	1.7449121047505636E-4
38-39	0.0047347518842366255	1.0737920644618852E-4	1.8120241087794312E-4
40-41	0.00505688950357519	1.140904068490753E-4	1.8120241087794312E-4
42-43	0.005318626319287775	1.2080160725196209E-4	2.0133601208660349E-4
44-45	0.0055803631350003595	1.2751280765484888E-4	2.0804721248949027E-4
46-47	0.005855522351518718	1.3422400805773564E-4	2.2146961329526382E-4
48-49	0.0061877267714616135	1.3422400805773564E-4	2.382476143024808E-4
50-51	0.006466241588181415	1.4093520846062243E-4	2.449588147053676E-4
52-53	0.00674811200510266	1.4093520846062243E-4	2.650924159140279E-4
54-55	0.007103805626455659	1.4429080866206582E-4	2.785148167198015E-4
56-57	0.007576945254859177	1.4764640886350921E-4	2.8858161732413164E-4
58-59	0.008191020091723318	1.4764640886350921E-4	2.9529281772701843E-4
60-61	0.008912474135033648	1.4764640886350921E-4	3.020040181299052E-4
62-63	0.009848686591236354	1.4764640886350921E-4	3.020040181299052E-4
64-65	0.011039924662748758	1.54357609266396E-4	3.0535961833134864E-4
66-67	0.012757991965887772	1.6778001007216958E-4	3.187820191371222E-4
68-69	0.01529818131838042	1.8120241087794312E-4	3.2549321954000894E-4
70-71	0.019043031143191246	1.8120241087794312E-4	3.3556002014433915E-4
72-73	0.024613327477587276	1.879136112808299E-4	3.389156203457825E-4
74-75	0.03261978955823121	1.879136112808299E-4	3.523380211515561E-4
76-77	0.043622802618764084	1.879136112808299E-4	3.5569362135299946E-4
78-79	0.059226343555475854	1.912692114822733E-4	3.6911602215877303E-4
80-81	0.08166188650232636	1.946248116837167E-4	3.758272225616598E-4
82-83	0.1132548623989159	1.946248116837167E-4	3.892496233674334E-4
84-85	0.15572333854838344	2.0133601208660349E-4	4.127388247775371E-4
86-87	0.21327523760333905	2.0133601208660349E-4	4.228056253818673E-4
88-89	0.2877091612717564	2.0133601208660349E-4	4.228056253818673E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCCGTCT	3820	0.0	34.1339	48-49
TATGCCG	3900	0.0	33.191624	46-47
ATGCCGT	4110	0.0	32.189182	46-47
TGCCGTC	4075	0.0	31.065369	48-49
AGAGCAC	9250	0.0	29.111246	8
CGTCTGA	4730	0.0	27.811863	16-17
AATCTCG	4260	0.0	27.710575	38-39
GAGCACA	9775	0.0	27.644804	9
CGTATGC	4690	0.0	27.447626	44-45
CTCGTAT	4530	0.0	27.108196	42-43
ACGTCTG	4900	0.0	27.041119	14-15
TCTCGTA	4455	0.0	26.926258	40-41
ATCTCGT	4585	0.0	25.592924	40-41
CAGTCAC	5340	0.0	25.126143	26-27
CACGTCT	5360	0.0	24.676125	14-15
ACACGTC	5420	0.0	24.446768	12-13
CCGTCTT	5285	0.0	24.177412	50-51
GTATGCC	5610	0.0	24.0895	44-45
CGGAAGA	11145	0.0	24.033823	4
TCGGAAG	11620	0.0	23.173986	3
>>END_MODULE