##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename H2K5YBCXX l01n01 10868-bar4.341000000043f2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 13185749 Sequences flagged as poor quality 0 Sequence length 101 %GC 40 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.978207684675326 38.0 38.0 38.0 32.0 38.0 2 36.958514984624685 38.0 38.0 38.0 32.0 38.0 3 36.94133097786102 38.0 38.0 38.0 32.0 38.0 4 36.93598467557664 38.0 38.0 38.0 32.0 38.0 5 36.93431871029852 38.0 38.0 38.0 32.0 38.0 6 38.88137996559771 40.0 38.0 40.0 38.0 40.0 7 38.84078272686671 40.0 38.0 40.0 38.0 40.0 8 38.8295652374393 40.0 38.0 40.0 38.0 40.0 9 38.84803980418556 40.0 38.0 40.0 38.0 40.0 10-11 38.84349209134801 40.0 38.0 40.0 38.0 40.0 12-13 38.83367107170021 40.0 38.0 40.0 38.0 40.0 14-15 38.82499860265807 40.0 38.0 40.0 38.0 40.0 16-17 38.81883088325131 40.0 38.0 40.0 38.0 40.0 18-19 38.78515645944724 40.0 38.0 40.0 38.0 40.0 20-21 38.77013194320626 40.0 38.0 40.0 38.0 40.0 22-23 38.75601814504432 40.0 38.0 40.0 38.0 40.0 24-25 38.7252513679731 40.0 38.0 40.0 38.0 40.0 26-27 38.664609647885754 40.0 38.0 40.0 38.0 40.0 28-29 38.616142018174315 40.0 38.0 40.0 38.0 40.0 30-31 38.598018588098405 40.0 38.0 40.0 38.0 40.0 32-33 38.56208615832138 40.0 38.0 40.0 38.0 40.0 34-35 38.54112462629161 40.0 38.0 40.0 38.0 40.0 36-37 38.51412301265556 40.0 38.0 40.0 38.0 40.0 38-39 38.485079156292144 40.0 38.0 40.0 38.0 40.0 40-41 38.45046159304261 40.0 38.0 40.0 38.0 40.0 42-43 38.39877158286572 40.0 38.0 40.0 38.0 40.0 44-45 38.37887047599647 40.0 38.0 40.0 38.0 40.0 46-47 38.34548826160729 40.0 38.0 40.0 38.0 40.0 48-49 38.28134086277541 40.0 38.0 40.0 38.0 40.0 50-51 38.26749072047405 40.0 38.0 40.0 38.0 40.0 52-53 38.24223572737506 40.0 38.0 40.0 38.0 40.0 54-55 38.17757918037117 40.0 38.0 40.0 38.0 40.0 56-57 38.168850097176886 40.0 38.0 40.0 38.0 40.0 58-59 38.14322201188571 40.0 38.0 40.0 38.0 40.0 60-61 38.08558539222914 40.0 38.0 40.0 38.0 40.0 62-63 38.0622578209247 40.0 38.0 40.0 38.0 40.0 64-65 38.00165269337373 40.0 38.0 40.0 38.0 40.0 66-67 37.94857387320205 40.0 38.0 40.0 38.0 40.0 68-69 37.90722271446241 40.0 38.0 40.0 38.0 40.0 70-71 37.889914975630134 40.0 38.0 40.0 38.0 40.0 72-73 37.85863832991208 40.0 38.0 40.0 38.0 40.0 74-75 37.78112911902085 40.0 38.0 40.0 32.0 40.0 76-77 37.72099374104573 40.0 38.0 40.0 32.0 40.0 78-79 37.64822077987378 40.0 38.0 40.0 32.0 40.0 80-81 37.61456334410734 40.0 38.0 40.0 32.0 40.0 82-83 37.543867549731154 40.0 38.0 40.0 32.0 40.0 84-85 37.51008899835724 40.0 38.0 40.0 32.0 40.0 86-87 37.470292282979145 40.0 38.0 40.0 32.0 40.0 88-89 37.4031143395798 40.0 38.0 40.0 32.0 40.0 90-91 37.34860605946617 38.0 38.0 40.0 32.0 40.0 92-93 37.27026314546106 38.0 38.0 40.0 32.0 40.0 94-95 37.201605574321185 38.0 38.0 40.0 32.0 40.0 96-97 37.11942309837689 38.0 38.0 40.0 32.0 40.0 98-99 37.101886627752435 38.0 38.0 40.0 32.0 40.0 100-101 36.28274442354393 38.0 38.0 40.0 29.5 40.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2201 1 0.13099578513467236 2201 2 0.07471281802551744 2201 3 0.044209434412721293 2201 4 0.06272681879604391 2201 5 0.013073845570218623 2201 6 0.04558345289026278 2201 7 0.03441882030684695 2201 8 -0.01216170701147945 2201 9 -0.0011262040764137282 2201 10-11 -0.018140121141243526 2201 12-13 0.01081269723642464 2201 14-15 -0.017998102095745594 2201 16-17 -0.11047193541716638 2201 18-19 -0.043215666479149206 2201 20-21 -0.036163601887828634 2201 22-23 -0.08328935603037735 2201 24-25 -0.07033070330272295 2201 26-27 -0.07015610747827594 2201 28-29 -0.13908911001750113 2201 30-31 -0.11718197061214397 2201 32-33 -0.14490774814080254 2201 34-35 -0.11972654377677827 2201 36-37 -0.13343387063815015 2201 38-39 -0.16472691563662778 2201 40-41 -0.1848989387282387 2201 42-43 -0.13584284367323107 2201 44-45 -0.1983390180381761 2201 46-47 -0.14329952353442366 2201 48-49 -0.14723063921663027 2201 50-51 -0.26501370523953227 2201 52-53 -0.20139737825011395 2201 54-55 -0.226586578272439 2201 56-57 -0.21790582670827519 2201 58-59 -0.20647240111098597 2201 60-61 -0.19157693633382422 2201 62-63 -0.1491453349575167 2201 64-65 -0.24553935750203948 2201 66-67 -0.20169181637302813 2201 68-69 -0.22588400304950085 2201 70-71 -0.2543876085803447 2201 72-73 -0.22319119441090152 2201 74-75 -0.22840628922494233 2201 76-77 -0.23217872751114044 2201 78-79 -0.24952723873298766 2201 80-81 -0.2739861641954491 2201 82-83 -0.27354788585016365 2201 84-85 -0.3577976850692508 2201 86-87 -0.4271509298481888 2201 88-89 -0.44403336900382584 2201 90-91 -0.3323470706746292 2201 92-93 -0.4212878993722313 2201 94-95 -0.4763464516530149 2201 96-97 -0.4864235334303686 2201 98-99 -0.4181564615628304 2201 100-101 -0.43599691861573575 1101 1 0.11708916210652376 1101 2 0.15976340740783712 1101 3 0.10930378862127554 1101 4 0.0761488374344026 1101 5 0.07570524718172322 1101 6 0.06655550188990134 1101 7 0.08009417695529208 1101 8 0.0963895702051829 1101 9 0.045739953943453315 1101 10-11 0.04312321692096077 1101 12-13 0.06324763584750315 1101 14-15 0.06916494395799333 1101 16-17 0.013611659825478739 1101 18-19 -0.0544270061247758 1101 20-21 -0.0386025092902571 1101 22-23 -0.07560613991797993 1101 24-25 -0.03513475141139111 1101 26-27 0.02735034603944797 1101 28-29 -0.08375579952586776 1101 30-31 -0.09131283271460688 1101 32-33 -0.12414163936905709 1101 34-35 -0.0973660559877132 1101 36-37 -0.061520890477211765 1101 38-39 -0.03379179023723822 1101 40-41 -0.032174589402565346 1101 42-43 -0.028934350935884368 1101 44-45 -0.0799625701555371 1101 46-47 -0.07348022428122647 1101 48-49 -0.0768906661741795 1101 50-51 -0.2191241987891246 1101 52-53 -0.14345887267635504 1101 54-55 -0.1626765455463186 1101 56-57 -0.12832651990952826 1101 58-59 -0.15325377691272024 1101 60-61 -0.12468590872184393 1101 62-63 -0.1505042758647832 1101 64-65 -0.06409506956051558 1101 66-67 -0.1513169101863454 1101 68-69 -0.13805877696286473 1101 70-71 -0.18181058527775917 1101 72-73 -0.183298989992565 1101 74-75 -0.15321132646913327 1101 76-77 -0.18001121975402157 1101 78-79 -0.16603151085097778 1101 80-81 -0.1788845661107885 1101 82-83 -0.2290479498790532 1101 84-85 -0.2138311958722383 1101 86-87 -0.26764732258835267 1101 88-89 -0.24484679777981455 1101 90-91 -0.2593099736461184 1101 92-93 -0.3160807094468723 1101 94-95 -0.22200917388430952 1101 96-97 -0.2839797465799023 1101 98-99 -0.29609124162641365 1101 100-101 -0.3724140680394896 2202 1 0.0978927292854408 2202 2 0.13118826857283494 2202 3 0.10032545820456562 2202 4 0.08170721007951443 2202 5 0.07471808974758432 2202 6 -0.024023864900037495 2202 7 0.016847619610011577 2202 8 -0.010516823599871827 2202 9 0.03112567633369423 2202 10-11 0.04560652596133252 2202 12-13 0.011044582621281052 2202 14-15 0.00432853461386884 2202 16-17 -0.0028778332987329236 2202 18-19 0.00989870411212479 2202 20-21 -0.009903904737029734 2202 22-23 -0.060064377603268326 2202 24-25 -0.021484685650627 2202 26-27 -0.06820643675366256 2202 28-29 -0.048250951898047845 2202 30-31 -0.02948073216776237 2202 32-33 -0.06006378407514035 2202 34-35 -0.08691814253704422 2202 36-37 -0.04333576833779773 2202 38-39 -0.0837244381961284 2202 40-41 -0.1261253233132038 2202 42-43 -0.1006373864734087 2202 44-45 -0.0948359268644694 2202 46-47 -0.08632889949691247 2202 48-49 -0.0833513757312403 2202 50-51 -0.14883341784217663 2202 52-53 -0.16473411868072674 2202 54-55 -0.15589601857644908 2202 56-57 -0.11310017059190614 2202 58-59 -0.1464125826681837 2202 60-61 -0.10447361872545002 2202 62-63 -0.10860542426646447 2202 64-65 -0.14178867879591905 2202 66-67 -0.09804742004292422 2202 68-69 -0.15088862685327342 2202 70-71 -0.2394393814046083 2202 72-73 -0.1975136734337113 2202 74-75 -0.22703935051610102 2202 76-77 -0.22190559589766679 2202 78-79 -0.21115922540960952 2202 80-81 -0.2010070227915861 2202 82-83 -0.24979004846380093 2202 84-85 -0.28595996791686673 2202 86-87 -0.20258529349676024 2202 88-89 -0.26112459479335826 2202 90-91 -0.3037491785723603 2202 92-93 -0.3319392344989751 2202 94-95 -0.2822659143808295 2202 96-97 -0.29786054689348873 2202 98-99 -0.32494897345745244 2202 100-101 -0.358977900597246 1102 1 0.02817841967758028 1102 2 0.06910442527329508 1102 3 0.023102481982611778 1102 4 0.03632517912388522 1102 5 -0.0031372654049732773 1102 6 -0.10091932734124498 1102 7 -0.14550449653219033 1102 8 -0.07122268930225317 1102 9 -0.04866516050095271 1102 10-11 -0.051864920524465674 1102 12-13 -0.051659498572725226 1102 14-15 -0.05703432287791088 1102 16-17 -0.007872408209564696 1102 18-19 -0.020978263375717177 1102 20-21 -0.06063512546020888 1102 22-23 -0.19944349739749612 1102 24-25 -0.10801356043172206 1102 26-27 -0.07245582428559061 1102 28-29 -0.18014174844905284 1102 30-31 -0.16348723050696634 1102 32-33 -0.11492769348122067 1102 34-35 -0.11697116910882954 1102 36-37 -0.1473880559910441 1102 38-39 -0.16174867157546657 1102 40-41 -0.13894689563385043 1102 42-43 -0.1692294823272107 1102 44-45 -0.20457966148460116 1102 46-47 -0.21101847062028156 1102 48-49 -0.18354953646862526 1102 50-51 -0.21614208103307675 1102 52-53 -0.2017013094559914 1102 54-55 -0.24305006524028983 1102 56-57 -0.2273230957000294 1102 58-59 -0.259317456694248 1102 60-61 -0.21339321776312659 1102 62-63 -0.2869205425279304 1102 64-65 -0.3306906151800959 1102 66-67 -0.334737147964951 1102 68-69 -0.31581850232154096 1102 70-71 -0.37146491345181687 1102 72-73 -0.35215045896179475 1102 74-75 -0.299715794847792 1102 76-77 -0.3346074884851191 1102 78-79 -0.42694900926587565 1102 80-81 -0.39179790241479395 1102 82-83 -0.4226399616365839 1102 84-85 -0.44103061216397776 1102 86-87 -0.40620512812732557 1102 88-89 -0.43364505191234315 1102 90-91 -0.3971119066885578 1102 92-93 -0.6328804073279883 1102 94-95 -0.5400388274630927 1102 96-97 -0.4945151225127873 1102 98-99 -0.5781946046684254 1102 100-101 -0.6089828704451321 2203 1 0.04753992365968429 2203 2 0.05806550679439937 2203 3 0.003223121067065904 2203 4 0.030968544291312128 2203 5 0.053681721453052944 2203 6 -0.09280470555813025 2203 7 -0.10439499985199063 2203 8 -0.05713949854722955 2203 9 -0.05681770287898047 2203 10-11 -0.062257917499692894 2203 12-13 -0.09582502895152345 2203 14-15 -0.049044042206745075 2203 16-17 -0.07006504696910554 2203 18-19 -0.04786266968069697 2203 20-21 -0.03736405690085576 2203 22-23 -0.14177129857138482 2203 24-25 -0.04310647806454426 2203 26-27 -0.09963005653238355 2203 28-29 -0.12189193593233227 2203 30-31 -0.13244302678669584 2203 32-33 -0.13567893468193404 2203 34-35 -0.15234228222694668 2203 36-37 -0.13510892400285002 2203 38-39 -0.13721507993948734 2203 40-41 -0.1811348444517762 2203 42-43 -0.13772873187957657 2203 44-45 -0.1722567992293662 2203 46-47 -0.12288622722713427 2203 48-49 -0.12344463807372108 2203 50-51 -0.1577822953894028 2203 52-53 -0.1843964173304542 2203 54-55 -0.2144599465241086 2203 56-57 -0.21948515223823506 2203 58-59 -0.24076742282778696 2203 60-61 -0.1846853785187932 2203 62-63 -0.2749124632782838 2203 64-65 -0.27950376221547657 2203 66-67 -0.24547245874557433 2203 68-69 -0.28243785900416185 2203 70-71 -0.2876749240165992 2203 72-73 -0.30460903606955725 2203 74-75 -0.2387684083848285 2203 76-77 -0.31626343542358626 2203 78-79 -0.2749394557138558 2203 80-81 -0.34898680089175116 2203 82-83 -0.3733577589249535 2203 84-85 -0.4301650492282434 2203 86-87 -0.46044994384524074 2203 88-89 -0.37289735308539207 2203 90-91 -0.36392029230667333 2203 92-93 -0.5734563433504718 2203 94-95 -0.5481797685826422 2203 96-97 -0.5338669796338849 2203 98-99 -0.4992507904405983 2203 100-101 -0.47036880744014553 1103 1 0.0629626875520728 1103 2 0.047862946430072384 1103 3 0.04363765531714847 1103 4 0.06186313084407402 1103 5 0.07102951606818664 1103 6 -0.06001973926506565 1103 7 -0.047394628396936866 1103 8 -0.013507491882933209 1103 9 0.004001473742796691 1103 10-11 0.011740610400238438 1103 12-13 -0.0268485153107747 1103 14-15 -0.01456483204062664 1103 16-17 -3.783390337872561E-4 1103 18-19 1.1485121673615595E-4 1103 20-21 -0.02239926544095283 1103 22-23 -0.06673283350755099 1103 24-25 -0.045005870484878585 1103 26-27 -0.0702949381375646 1103 28-29 -0.09853758009700897 1103 30-31 -0.058752707538978655 1103 32-33 -0.028633315822347072 1103 34-35 -0.06741836533663559 1103 36-37 -0.12446112474221849 1103 38-39 -0.09030633759745399 1103 40-41 -0.06466490580029927 1103 42-43 -0.0630936454009543 1103 44-45 -0.1079897479318177 1103 46-47 -0.024568716293224213 1103 48-49 -0.08762435280532799 1103 50-51 -0.0859171294640575 1103 52-53 -0.08901429089481638 1103 54-55 -0.041842841777231854 1103 56-57 -0.0834542420507205 1103 58-59 -0.14531517632813973 1103 60-61 -0.13659624009088844 1103 62-63 -0.09413343771119997 1103 64-65 -0.21501406293143788 1103 66-67 -0.10071487706223792 1103 68-69 -0.17571521942696933 1103 70-71 -0.13630324195077037 1103 72-73 -0.10164754768621975 1103 74-75 -0.09476029790776863 1103 76-77 -0.0823960742529124 1103 78-79 -0.09450767653220993 1103 80-81 -0.21254277374973185 1103 82-83 -0.2024881804936598 1103 84-85 -0.3206721357659177 1103 86-87 -0.1869224214306584 1103 88-89 -0.2447139595278216 1103 90-91 -0.24778550386074727 1103 92-93 -0.3090193997727084 1103 94-95 -0.19700707132592754 1103 96-97 -0.24037109542261703 1103 98-99 -0.33701585007176504 1103 100-101 -0.3512801199071589 2204 1 0.04979061759571124 2204 2 0.06277593654654368 2204 3 0.07177834086898827 2204 4 0.07417541762428215 2204 5 0.09544546820518462 2204 6 -0.006010535453022214 2204 7 0.018602590701746635 2204 8 0.06701390827599596 2204 9 0.07887160884749989 2204 10-11 0.05446792887828167 2204 12-13 0.03854324622248839 2204 14-15 0.03667736129499133 2204 16-17 0.05661792919421771 2204 18-19 0.05760787753717267 2204 20-21 0.09489904334161281 2204 22-23 0.00484956045490037 2204 24-25 0.008343601068922624 2204 26-27 0.06559143596350481 2204 28-29 -0.036550679597134206 2204 30-31 -0.02876338050955951 2204 32-33 0.008777965471949756 2204 34-35 0.01487994489504274 2204 36-37 -0.02022369706310201 2204 38-39 0.021909055891640605 2204 40-41 0.06977729671859834 2204 42-43 0.06890428620897637 2204 44-45 0.05363778237020256 2204 46-47 0.04339455399803427 2204 48-49 0.02182213082598139 2204 50-51 0.024614051007972648 2204 52-53 0.04214243731843936 2204 54-55 0.043375319612515284 2204 56-57 0.04498842029441619 2204 58-59 0.018191307921739508 2204 60-61 0.04253411009375441 2204 62-63 0.06798908068753207 2204 64-65 0.03331799559095572 2204 66-67 0.0849495519986263 2204 68-69 0.04312688374858453 2204 70-71 0.06717736868822044 2204 72-73 0.04061076436886424 2204 74-75 0.04327697290211319 2204 76-77 0.03313925152835395 2204 78-79 0.07621967080400083 2204 80-81 0.08967525133998322 2204 82-83 -0.01139020620095721 2204 84-85 0.013958159331494358 2204 86-87 -0.01494260546797932 2204 88-89 -0.020427147726472583 2204 90-91 -0.0030512669559854544 2204 92-93 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1214 100-101 0.6105998600018836 1215 1 -0.08766188496434779 1215 2 -0.052956927516518704 1215 3 -0.010101620185473337 1215 4 -0.04187285549146935 1215 5 -0.06659441175185776 1215 6 0.010162212231477952 1215 7 -0.022991396615474002 1215 8 0.027227564808065097 1215 9 0.004914090517701197 1215 10-11 -0.006511047286899441 1215 12-13 -5.159101159080137E-4 1215 14-15 0.004525856449561161 1215 16-17 -0.03281399240627536 1215 18-19 0.008842753719612517 1215 20-21 -0.02999463940225411 1215 22-23 0.009290479837581245 1215 24-25 -0.07209193395843982 1215 26-27 -0.0028516485552430026 1215 28-29 3.7579767236906036E-4 1215 30-31 0.037826004855745055 1215 32-33 0.07070567374570658 1215 34-35 0.007022523528299018 1215 36-37 0.08655540037031528 1215 38-39 0.044559462423634955 1215 40-41 0.050369655961134185 1215 42-43 0.09482501297098622 1215 44-45 0.11834172728075742 1215 46-47 0.08606108954842995 1215 48-49 0.11704403079228598 1215 50-51 0.1381871825367682 1215 52-53 0.12790041959369347 1215 54-55 0.11191284076502228 1215 56-57 0.1812198310567723 1215 58-59 0.1371076777875544 1215 60-61 0.18608047730003108 1215 62-63 0.17113381701359032 1215 64-65 0.2207076525215328 1215 66-67 0.2491688462438546 1215 68-69 0.24897340958263925 1215 70-71 0.22755811257550818 1215 72-73 0.269234089380042 1215 74-75 0.22258329771604224 1215 76-77 0.2999406702178007 1215 78-79 0.22484979926072413 1215 80-81 0.2728257805429877 1215 82-83 0.31337197379877324 1215 84-85 0.2942210388565414 1215 86-87 0.3555502728861839 1215 88-89 0.40240881838140297 1215 90-91 0.372990506171071 1215 92-93 0.471677175625544 1215 94-95 0.41259483747369785 1215 96-97 0.4927444318174423 1215 98-99 0.3719487583031764 1215 100-101 0.4942835961509999 1212 1 -0.09290580988041341 1212 2 -0.06356828569979456 1212 3 -0.08416462216327858 1212 4 -0.056573761171478054 1212 5 -0.01976599953736269 1212 6 -0.10587518735815138 1212 7 -0.11008245983035181 1212 8 -0.19196216913858422 1212 9 -0.1056176502344286 1212 10-11 -0.20570753777008122 1212 12-13 -0.22175105776950943 1212 14-15 -0.10941061393958051 1212 16-17 -0.016641607082434007 1212 18-19 -0.019067536321941247 1212 20-21 -0.02761874376512452 1212 22-23 0.025082462804235206 1212 24-25 0.006951803565314663 1212 26-27 0.030315328090914306 1212 28-29 0.06164434158332455 1212 30-31 4.2279946114121003E-4 1212 32-33 -0.001396474082227428 1212 34-35 0.013033830104582478 1212 36-37 -0.013920734123871625 1212 38-39 -0.019797168755793848 1212 40-41 -0.11272639831639708 1212 42-43 -0.17607383863722248 1212 44-45 0.04664609760587979 1212 46-47 -0.016402699647194652 1212 48-49 0.036820570746307624 1212 50-51 0.03730320334680215 1212 52-53 -0.0359058003891235 1212 54-55 -0.06889994050843029 1212 56-57 -0.04695950635299084 1212 58-59 0.08335149989702018 1212 60-61 -0.23211529088199256 1212 62-63 -0.15159517391108324 1212 64-65 -0.07666053550918406 1212 66-67 -0.07603989188385185 1212 68-69 0.1200039242600468 1212 70-71 0.06967529994228272 1212 72-73 0.18291269430600465 1212 74-75 0.13214145245914466 1212 76-77 0.17351496818331213 1212 78-79 0.2578472481334515 1212 80-81 0.2194703063245811 1212 82-83 0.1739436680799642 1212 84-85 0.2707114012099865 1212 86-87 0.27441685114860803 1212 88-89 0.2968287471702453 1212 90-91 0.26194598671299474 1212 92-93 0.34725475151016383 1212 94-95 0.26013979248941155 1212 96-97 0.30494524363380293 1212 98-99 0.2889873698171286 1212 100-101 0.2611766982688266 1213 1 -0.21237469518315777 1213 2 -0.1182692275040722 1213 3 -0.1794393844527491 1213 4 -0.16337959054817475 1213 5 -0.1283060341205271 1213 6 -0.030870113662814447 1213 7 -0.031163848897186597 1213 8 -0.032016818452433427 1213 9 -0.07589087931974348 1213 10-11 -0.06396419184250135 1213 12-13 -0.12846428127880927 1213 14-15 -0.14782706823281444 1213 16-17 -0.23726546751835542 1213 18-19 -0.28280014168366563 1213 20-21 -0.16294845293109006 1213 22-23 -0.21593443182791816 1213 24-25 -0.2788899285438404 1213 26-27 -0.13850207657370817 1213 28-29 -0.15081066593543113 1213 30-31 -0.12556268096098222 1213 32-33 -0.11764258777952818 1213 34-35 -0.12662509114642262 1213 36-37 -0.031126709276009024 1213 38-39 -0.00578938350820124 1213 40-41 0.05087927556112959 1213 42-43 0.06813959165289418 1213 44-45 0.10583763468205376 1213 46-47 0.1018832127350322 1213 48-49 0.10120864274072972 1213 50-51 0.13428244820071455 1213 52-53 0.14095831192803843 1213 54-55 0.15746312674180274 1213 56-57 0.1269148158261899 1213 58-59 0.14715879180254632 1213 60-61 0.18938086521379205 1213 62-63 0.20880591834893636 1213 64-65 0.16965504713058266 1213 66-67 0.2071371599770444 1213 68-69 0.2414424523894354 1213 70-71 0.18562117460768235 1213 72-73 0.18904348011751182 1213 74-75 0.22221002333399298 1213 76-77 0.27610721462194476 1213 78-79 0.3057450571174698 1213 80-81 0.27929238201672035 1213 82-83 0.2832331268037507 1213 84-85 0.32120428263721834 1213 86-87 0.3389849014737436 1213 88-89 0.32165304735332967 1213 90-91 0.3503009413631091 1213 92-93 0.431660553867502 1213 94-95 0.3535926022138085 1213 96-97 0.40785203228524836 1213 98-99 0.45571904355558956 1213 100-101 0.17023318729317083 >>END_MODULE >>Per sequence quality scores pass #Quality Count 13 15.0 14 33.0 15 87.0 16 131.0 17 221.0 18 464.0 19 1141.0 20 2641.0 21 5646.0 22 10459.0 23 17660.0 24 27558.0 25 39088.0 26 52919.0 27 70240.0 28 90601.0 29 112841.0 30 137767.0 31 168098.0 32 203218.0 33 247596.0 34 308824.0 35 402788.0 36 565116.0 37 913946.0 38 2063544.0 39 7743107.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 18.294745133745664 30.619290261707636 29.504843616709834 21.58112098783686 2 20.131288970654094 30.997526643021395 29.65920791753323 19.211976468791285 3 20.078851796739038 29.76869194158026 29.764922720734333 20.387533540946364 4 19.977712301364146 29.507917980237604 30.166394036470738 20.34797568192751 5 20.45514012099756 29.865495066888425 30.083713868152557 19.59565094396146 6 20.325496867868484 29.959822532644903 29.453776194283694 20.260904405202922 7 20.50965781314357 30.18856190876984 29.685003104488032 19.616777173598557 8 19.95765878807666 30.111472739289844 29.872737469697363 20.05813100293613 9 19.899807838493626 29.80268956334053 30.280306126620303 20.017196471545542 10-11 20.056812851511125 30.039905203716526 29.88602316030739 20.017258784464957 12-13 19.908703631150566 30.045038055511192 30.02081491381066 20.025443399527582 14-15 19.890429850259082 29.957576036114254 30.112763000667087 20.039231112959577 16-17 19.97377209365326 29.83285369298295 30.12420240484032 20.069171808523468 18-19 19.93940579333036 29.714633579025357 30.152928741476874 20.193031886167407 20-21 20.027579046598465 29.676021094008032 30.052606092872335 20.24379376652117 22-23 20.097640462918907 29.654227504568958 30.0004770311402 20.24765500137194 24-25 20.144660009730206 29.626107588156756 29.917657288230586 20.311575113882455 26-27 20.19650365232438 29.608878128085347 29.863912104553997 20.330706115036275 28-29 20.238267730371867 29.63840593474119 29.813624320662353 20.30970201422459 30-31 20.305238086389956 29.547322824523558 29.833744708654127 20.313694380432363 32-33 20.347724432891923 29.60345446152552 29.729726860049237 20.31909424553332 34-35 20.363127360330115 29.575966888750248 29.72695899910247 20.333946751817162 36-37 20.349619508066233 29.570364746655358 29.717229665287693 20.362786079990716 38-39 20.36349243621914 29.53349782732621 29.748429161247497 20.354580575207155 40-41 20.412019703865578 29.558665388187784 29.670775001217347 20.35853990672929 42-43 20.397769390541924 29.5136020565268 29.706894488653052 20.38173406427822 44-45 20.400192350210876 29.440985992035213 29.717129142763525 20.441692514990383 46-47 20.412919051512674 29.512478818926663 29.675901134258805 20.398700995301862 48-49 20.424139184662035 29.451075178652847 29.683955524537716 20.440830112147406 50-51 20.388831077724536 29.49036720710235 29.656255056949384 20.464546658223735 52-53 20.436155826707914 29.49630555248776 29.681619573699624 20.385919047104696 54-55 20.440576412785656 29.43777256473441 29.69442789242495 20.42722313005498 56-57 20.43856572634246 29.45120483289314 29.676362478514246 20.433866962250153 58-59 20.424761146089203 29.46416107694077 29.69513368185145 20.415944095118572 60-61 20.424060913255147 29.444459339191738 29.608405633005503 20.52307411454762 62-63 20.418534658859812 29.429411983752157 29.682056877162744 20.469996480225287 64-65 20.442901677556748 29.44153237503645 29.650776664900846 20.464789282505958 66-67 20.428724486766143 29.478665933637053 29.59604142775137 20.49656815184543 68-69 20.386905032787173 29.45207162633726 29.651481172025413 20.509542168850153 70-71 20.502519834617758 29.456143705942427 29.569662068708435 20.47167439073138 72-73 20.462270260054325 29.49515846287145 29.575413084347325 20.4671581927269 74-75 20.474910713730342 29.445831175863113 29.62064125906346 20.458616851343084 76-77 20.45870756428331 29.491046413804934 29.582051419971698 20.46819460194006 78-79 20.46403375905552 29.483800955798973 29.603350747557005 20.4488145375885 80-81 20.473650235763476 29.47841268941801 29.568836383976176 20.479100690842337 82-83 20.465835386003466 29.513506081984875 29.610533465095727 20.410125066915928 84-85 20.46190856404766 29.494087935091155 29.575658637816677 20.468344863044507 86-87 20.407207446108487 29.468310977445476 29.60539165805804 20.519089918387994 88-89 20.474542877889725 29.4642589625759 29.56412316304068 20.497074996493698 90-91 20.463383495650397 29.450052207065347 29.544382907330203 20.542181389954052 92-93 20.425040822266297 29.44204929711062 29.593304260845617 20.53960561977746 94-95 20.47317557906235 29.45269078620491 29.555304389381735 20.518829245351004 96-97 20.491271900270053 29.500373328932067 29.483072164892153 20.525282605905726 98-99 20.471606708862826 29.484431271470495 29.51244743650952 20.531514583157158 100-101 20.563565031046995 29.46067517730238 29.40874027021547 20.56701952143515 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 667.0 1 611.5 2 662.5 3 849.0 4 1014.0 5 1210.5 6 1505.5 7 1913.0 8 2411.5 9 2872.0 10 3430.5 11 4154.0 12 5225.0 13 6477.5 14 7754.0 15 9578.0 16 12093.0 17 15965.5 18 21651.5 19 29998.0 20 41755.5 21 56806.5 22 75488.0 23 97700.5 24 123694.5 25 153295.5 26 185487.0 27 219948.0 28 256290.0 29 292328.5 30 326863.5 31 360318.5 32 392595.0 33 423884.0 34 452629.5 35 478952.5 36 503840.5 37 523210.0 38 534517.5 39 535371.0 40 525968.0 41 508941.0 42 484101.5 43 455177.0 44 427627.5 45 404622.5 46 385340.0 47 369826.0 48 353075.5 49 331609.5 50 308313.5 51 283748.0 52 258517.5 53 235754.5 54 216156.5 55 198049.0 56 181548.5 57 165198.5 58 148615.0 59 130767.5 60 112180.0 61 94451.5 62 78013.0 63 64229.0 64 53150.5 65 44019.5 66 36320.5 67 29685.0 68 24318.0 69 19715.5 70 15777.0 71 12527.0 72 10012.5 73 8195.0 74 6790.0 75 5798.0 76 5009.5 77 4476.5 78 4090.5 79 3563.0 80 2981.0 81 2591.5 82 2292.5 83 1920.0 84 1608.0 85 1283.5 86 965.0 87 735.0 88 505.5 89 333.5 90 228.0 91 147.0 92 88.5 93 47.0 94 27.0 95 13.0 96 9.0 97 5.0 98 1.5 99 0.5 100 1.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.08175493102439611 2 1.9718257946514832E-4 3 0.0 4 0.0 5 0.0027226363856918557 6 0.0 7 0.0 8 1.0617523509661833E-4 9 0.0036857974469254647 10-11 0.0 12-13 8.342339900448583E-5 14-15 3.109417599258108E-4 16-17 0.001327190438707729 18-19 0.0 20-21 1.0617523509661833E-4 22-23 2.0476652482919247E-4 24-25 3.0335781456176665E-5 26-27 0.0033900235777277426 28-29 0.001543332881582988 30-31 0.0023055193906694263 32-33 0.0027719320305581428 34-35 0.004724797961799516 36-37 0.006400849887253276 38-39 0.00813757337561939 40-41 0.010094231279542785 42-43 0.017871567250370077 44-45 0.01110289601296066 46-47 0.013719357163555896 48-49 0.014712854006245684 50-51 0.011683067833310037 52-53 0.016540584838980327 54-55 0.013082305752976187 56-57 0.010852625815947202 58-59 0.00810344562148119 60-61 0.0048423491149422 62-63 0.0018656505595548648 64-65 0.0015471248542650098 66-67 0.00218417626484472 68-69 0.01004872760735852 70-71 0.004986444076859039 72-73 0.0016343402259515179 74-75 0.021773507140170803 76-77 0.03493165234678743 78-79 0.038287548170376974 80-81 0.025508600231962553 82-83 0.04523444212384143 84-85 0.02052974010046756 86-87 0.017230723867108347 88-89 0.017791935824047613 90-91 0.005703126913761213 92-93 0.018049789966425117 94-95 0.02122746307395962 96-97 0.012627269031133535 98-99 0.0038223084634782596 100-101 9.479931705055208E-5 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1.3185749E7 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 94.57450986777552 #Duplication Level Percentage of deduplicated Percentage of total 1 98.09900026528034 92.77664868607668 2 1.2885597316295512 2.437298101084343 3 0.18508012234460272 0.5251158557102618 4 0.1090981735958663 0.41271625181194166 5 0.08465941860202043 0.4003311509988459 6 0.04190148559566692 0.2377687477765113 7 0.037817322345006364 0.2503588307703456 8 0.01820510775907862 0.1377391314723918 9 0.019130359056454264 0.16283198982228503 >10 0.11323265330364489 2.443759889235378 >50 0.0032644801742377 0.17800749212665556 >100 4.034370163867614E-5 0.01392652320186292 >500 0.0 0.0 >1k 1.0536611911825728E-5 0.02349734991243349 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 1.4409496191683917E-4 0.0 1.5167890728088332E-5 2 1.4409496191683917E-4 0.0 2.27518360921325E-5 3 1.4409496191683917E-4 7.583945364044166E-6 3.791972682022083E-5 4 1.5167890728088332E-4 7.583945364044166E-6 3.791972682022083E-5 5 1.6684679800897166E-4 7.583945364044166E-6 6.067156291235333E-5 6 1.8201468873706E-4 7.583945364044166E-6 6.82555082763975E-5 7 1.9718257946514832E-4 7.583945364044166E-6 8.342339900448583E-5 8 1.9718257946514832E-4 7.583945364044166E-6 8.342339900448583E-5 9 2.1993441555728083E-4 7.583945364044166E-6 9.859128973257416E-5 10-11 2.7681400578761204E-4 7.583945364044166E-6 1.1375918046066249E-4 12-13 3.0714978724378876E-4 1.137591804606625E-5 1.4030298923481708E-4 14-15 3.5644543211007585E-4 1.5167890728088332E-5 1.6305482532694957E-4 16-17 4.0953304965838495E-4 1.5167890728088332E-5 1.8580666141908208E-4 18-19 4.474527764786058E-4 1.5167890728088332E-5 1.9339060678312623E-4 20-21 5.081243393909591E-4 1.5167890728088332E-5 2.3131033360334707E-4 22-23 5.574199842572462E-4 1.5167890728088332E-5 2.654380877415458E-4 24-25 6.256754925336437E-4 2.27518360921325E-5 2.692300604235679E-4 26-27 6.635952193538646E-4 2.27518360921325E-5 2.919818965157004E-4 28-29 7.204748095841957E-4 2.27518360921325E-5 3.374855686999654E-4 30-31 8.266500446808141E-4 2.654380877415458E-5 3.5644543211007585E-4 32-33 9.404092251414765E-4 3.0335781456176665E-5 3.8678121356625246E-4 34-35 0.001050376432920117 3.0335781456176665E-5 4.0953304965838495E-4 36-37 0.0011527596953347131 3.0335781456176665E-5 4.209089677044512E-4 38-39 0.0012589349304313316 3.0335781456176665E-5 4.284929130684954E-4 40-41 0.0013878620016200824 3.0335781456176665E-5 4.436608037965837E-4 42-43 0.001509205127444789 3.0335781456176665E-5 4.5503672184264997E-4 44-45 0.0016229643079054515 3.0335781456176665E-5 4.739965852527604E-4 46-47 0.001740515461048136 3.412775413819875E-5 4.929564486628708E-4 48-49 0.0018921943683290194 4.5503672184265E-5 5.00540394026915E-4 50-51 0.002047665248291925 4.5503672184265E-5 5.157082847550034E-4 52-53 0.0022524317731211175 4.5503672184265E-5 5.4604406621118E-4 54-55 0.0025292457789087295 4.929564486628708E-5 5.725878749853346E-4 56-57 0.0028060597846963414 5.308761754830916E-5 5.763798476673567E-4 58-59 0.003226968752400793 6.067156291235333E-5 5.839637930314008E-4 60-61 0.003651669692787266 6.067156291235333E-5 6.029236564415112E-4 62-63 0.004372144502371462 6.067156291235333E-5 6.218835198516215E-4 64-65 0.005176042710960144 6.82555082763975E-5 6.446353559437541E-4 66-67 0.006385681996525188 7.583945364044166E-5 6.82555082763975E-4 68-69 0.007898679096652 8.342339900448583E-5 7.128908642201517E-4 70-71 0.010018391825902343 8.342339900448583E-5 7.470186183583503E-4 72-73 0.013036802080791922 8.342339900448583E-5 7.659784817684608E-4 74-75 0.017530289708988088 8.342339900448583E-5 7.811463724965492E-4 76-77 0.02371878912604813 9.859128973257416E-5 8.038982085886816E-4 78-79 0.03220901596109557 9.859128973257416E-5 8.494018807729466E-4 80-81 0.04399446705682021 9.859128973257416E-5 8.835296349111453E-4 82-83 0.05949605138092648 9.859128973257416E-5 9.062814710032779E-4 84-85 0.08052633187542096 1.0238326241459624E-4 9.214493617313662E-4 86-87 0.10673644705355759 1.0617523509661833E-4 9.59369088551587E-4 88-89 0.13993516788466093 1.0617523509661833E-4 9.707450065976533E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCTCGTA 1125 0.0 11.613104 40-41 CTCGTAT 1125 0.0 11.19013 42-43 TATGCCG 1070 0.0 10.6548815 46-47 TAGATCG 2385 0.0 9.361235 94-95 TATCTCG 1330 0.0 9.286568 38-39 AGATCGG 5145 0.0 9.09441 94-95 ATGCCGT 1430 0.0 8.803006 46-47 GATCGGA 4600 0.0 8.674514 94-95 CGCCAAT 1500 0.0 8.39045 32-33 ATCTCGT 1685 0.0 8.176477 40-41 GTCACGC 1565 0.0 8.041583 28-29 ACGCCAA 1605 0.0 7.8415413 32-33 TCGGAAG 4510 0.0 7.5836735 94-95 ATCGGAA 4520 0.0 7.4617996 94-95 CGTATGC 1675 0.0 7.373323 44-45 AGTCACG 1950 0.0 6.940972 28-29 GCCGTCT 1885 0.0 6.929833 48-49 TGCCGTC 1735 0.0 6.844504 48-49 CGTCTGA 2030 0.0 6.784113 16-17 CGGAAGA 4595 0.0 6.668035 94-95 >>END_MODULE