FastQCFastQC Report
Fri 3 Apr 2015
H2K5YBCXX l01n01 10868-bar4.341000000043f2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2K5YBCXX l01n01 10868-bar4.341000000043f2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13185749
Sequences flagged as poor quality0
Sequence length101
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGTA11250.011.61310440-41
CTCGTAT11250.011.1901342-43
TATGCCG10700.010.654881546-47
TAGATCG23850.09.36123594-95
TATCTCG13300.09.28656838-39
AGATCGG51450.09.0944194-95
ATGCCGT14300.08.80300646-47
GATCGGA46000.08.67451494-95
CGCCAAT15000.08.3904532-33
ATCTCGT16850.08.17647740-41
GTCACGC15650.08.04158328-29
ACGCCAA16050.07.841541332-33
TCGGAAG45100.07.583673594-95
ATCGGAA45200.07.461799694-95
CGTATGC16750.07.37332344-45
AGTCACG19500.06.94097228-29
GCCGTCT18850.06.92983348-49
TGCCGTC17350.06.84450448-49
CGTCTGA20300.06.78411316-17
CGGAAGA45950.06.66803594-95
Produced by FastQC (version 0.11.2)