##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename H2K5YBCXX l01n01 10551-bar6.34100000004444.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 13509011 Sequences flagged as poor quality 0 Sequence length 101 %GC 40 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.99957554257673 38.0 38.0 38.0 32.0 38.0 2 36.98439545278333 38.0 38.0 38.0 32.0 38.0 3 36.96653211696992 38.0 38.0 38.0 32.0 38.0 4 36.95904644684944 38.0 38.0 38.0 32.0 38.0 5 36.95583621924654 38.0 38.0 38.0 32.0 38.0 6 38.89614798596285 40.0 38.0 40.0 38.0 40.0 7 38.85708487468106 40.0 38.0 40.0 38.0 40.0 8 38.84778411979974 40.0 38.0 40.0 38.0 40.0 9 38.86511514425445 40.0 38.0 40.0 38.0 40.0 10-11 38.86436823539488 40.0 38.0 40.0 38.0 40.0 12-13 38.853621445715014 40.0 38.0 40.0 38.0 40.0 14-15 38.844886905488494 40.0 38.0 40.0 38.0 40.0 16-17 38.83919674060522 40.0 38.0 40.0 38.0 40.0 18-19 38.805661569155575 40.0 38.0 40.0 38.0 40.0 20-21 38.79095975271617 40.0 38.0 40.0 38.0 40.0 22-23 38.77757394675302 40.0 38.0 40.0 38.0 40.0 24-25 38.74826173433422 40.0 38.0 40.0 38.0 40.0 26-27 38.68809507964721 40.0 38.0 40.0 38.0 40.0 28-29 38.639300760063044 40.0 38.0 40.0 38.0 40.0 30-31 38.62423877662103 40.0 38.0 40.0 38.0 40.0 32-33 38.58847809066111 40.0 38.0 40.0 38.0 40.0 34-35 38.56894116823208 40.0 38.0 40.0 38.0 40.0 36-37 38.540984606497105 40.0 38.0 40.0 38.0 40.0 38-39 38.51340423810447 40.0 38.0 40.0 38.0 40.0 40-41 38.47960069023557 40.0 38.0 40.0 38.0 40.0 42-43 38.43014736608032 40.0 38.0 40.0 38.0 40.0 44-45 38.41027292819585 40.0 38.0 40.0 38.0 40.0 46-47 38.37756187333032 40.0 38.0 40.0 38.0 40.0 48-49 38.316745282093564 40.0 38.0 40.0 38.0 40.0 50-51 38.30203395348482 40.0 38.0 40.0 38.0 40.0 52-53 38.27826470790497 40.0 38.0 40.0 38.0 40.0 54-55 38.21448024581518 40.0 38.0 40.0 38.0 40.0 56-57 38.20746700110023 40.0 38.0 40.0 38.0 40.0 58-59 38.18005981340899 40.0 38.0 40.0 38.0 40.0 60-61 38.1241862191096 40.0 38.0 40.0 38.0 40.0 62-63 38.102487258319655 40.0 38.0 40.0 38.0 40.0 64-65 38.040228222480536 40.0 38.0 40.0 38.0 40.0 66-67 37.98890647879404 40.0 38.0 40.0 38.0 40.0 68-69 37.94960245424332 40.0 38.0 40.0 38.0 40.0 70-71 37.93033635104746 40.0 38.0 40.0 38.0 40.0 72-73 37.90210771166002 40.0 38.0 40.0 38.0 40.0 74-75 37.824880481628156 40.0 38.0 40.0 35.0 40.0 76-77 37.765017402088134 40.0 38.0 40.0 32.0 40.0 78-79 37.692135752942974 40.0 38.0 40.0 32.0 40.0 80-81 37.66121413329222 40.0 38.0 40.0 32.0 40.0 82-83 37.59371196751561 40.0 38.0 40.0 32.0 40.0 84-85 37.55802848928023 40.0 38.0 40.0 32.0 40.0 86-87 37.521028741482255 40.0 38.0 40.0 32.0 40.0 88-89 37.45525586588093 40.0 38.0 40.0 32.0 40.0 90-91 37.40042342848044 40.0 38.0 40.0 32.0 40.0 92-93 37.322728843732534 38.0 38.0 40.0 32.0 40.0 94-95 37.25374833139154 38.0 38.0 40.0 32.0 40.0 96-97 37.17462421934515 38.0 38.0 40.0 32.0 40.0 98-99 37.157770690985444 38.0 38.0 40.0 32.0 40.0 100-101 36.34542043825414 38.0 38.0 40.0 29.5 40.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2201 1 0.11714003877647627 2201 2 0.12300774406511295 2201 3 0.06595723051155034 2201 4 0.09859440996333291 2201 5 0.06653967188063348 2201 6 0.04193695337811931 2201 7 0.07777446278606703 2201 8 0.038708381874933195 2201 9 0.0173084447088101 2201 10-11 0.034649908700821186 2201 12-13 0.02448749552574725 2201 14-15 -0.015962684780220115 2201 16-17 -0.10707274521462296 2201 18-19 -0.08014412104949287 2201 20-21 -0.061926773860101036 2201 22-23 -0.08114415225931282 2201 24-25 -0.08986131908091011 2201 26-27 -0.056961747362429094 2201 28-29 -0.0922250855621698 2201 30-31 -0.0652587225669734 2201 32-33 -0.12875334223235768 2201 34-35 -0.12417850040367284 2201 36-37 -0.14402910677709002 2201 38-39 -0.15832919458425465 2201 40-41 -0.15733160733496732 2201 42-43 -0.13249826716609192 2201 44-45 -0.17485065910373976 2201 46-47 -0.16705593530450358 2201 48-49 -0.13273598925200503 2201 50-51 -0.22617167961060858 2201 52-53 -0.14056419900855843 2201 54-55 -0.13181069648028654 2201 56-57 -0.19334518636773623 2201 58-59 -0.1494665888643283 2201 60-61 -0.18413158512652217 2201 62-63 -0.1369635017653721 2201 64-65 -0.21998294682818909 2201 66-67 -0.11615646527038592 2201 68-69 -0.13954637764904732 2201 70-71 -0.23865114901555273 2201 72-73 -0.20322664830244008 2201 74-75 -0.15876494881988634 2201 76-77 -0.20924840674791056 2201 78-79 -0.2299400337776305 2201 80-81 -0.22581060699430822 2201 82-83 -0.2705854576891653 2201 84-85 -0.2614757128038079 2201 86-87 -0.3534480698691169 2201 88-89 -0.36084638279449877 2201 90-91 -0.2609372291928054 2201 92-93 -0.3995672384881459 2201 94-95 -0.3710683910675385 2201 96-97 -0.4381727951746086 2201 98-99 -0.3684396312858311 2201 100-101 -0.38444239073754716 1101 1 0.10797210645895916 1101 2 0.13686722082277214 1101 3 0.08121924105240907 1101 4 0.06558274213998772 1101 5 0.12125632934775865 1101 6 0.09452522364767191 1101 7 0.0982685172065203 1101 8 0.056822000506620896 1101 9 0.0504319028473148 1101 10-11 0.08823497390653756 1101 12-13 0.05273188801088935 1101 14-15 0.03392259852068946 1101 16-17 -0.009976764964164886 1101 18-19 -0.050776695057628274 1101 20-21 -0.04933491723968331 1101 22-23 -0.09794929769936545 1101 24-25 -0.06474712276389027 1101 26-27 -0.005586659142359451 1101 28-29 -0.08978397322281495 1101 30-31 -0.09200943481698687 1101 32-33 -0.0947715169132266 1101 34-35 -0.06090955323089986 1101 36-37 -0.07351211269010349 1101 38-39 -0.04730908163318048 1101 40-41 -0.048028614440084993 1101 42-43 -0.046876509758668305 1101 44-45 -0.13587964916089135 1101 46-47 -0.12174702058398879 1101 48-49 -0.12230021027798443 1101 50-51 -0.1858668263191774 1101 52-53 -0.1255490393602372 1101 54-55 -0.17281471676857763 1101 56-57 -0.10309831588435259 1101 58-59 -0.17294506512682517 1101 60-61 -0.11704286818547871 1101 62-63 -0.16009407505745799 1101 64-65 -0.14293763097708023 1101 66-67 -0.1922284033789552 1101 68-69 -0.2017702996500219 1101 70-71 -0.20735183822182535 1101 72-73 -0.20058532354924097 1101 74-75 -0.17656128743605848 1101 76-77 -0.2324656083510277 1101 78-79 -0.19862529518575656 1101 80-81 -0.20043055053346137 1101 82-83 -0.25612506207143326 1101 84-85 -0.23157585023365357 1101 86-87 -0.3034820722464744 1101 88-89 -0.3395778161740779 1101 90-91 -0.29112844450657605 1101 92-93 -0.32655672652524004 1101 94-95 -0.2752239480869605 1101 96-97 -0.28370412731844397 1101 98-99 -0.2943349823270083 1101 100-101 -0.3958217877212391 2202 1 0.05137320774025511 2202 2 0.10351591704822027 2202 3 0.07179563812265144 2202 4 0.0756835978207917 2202 5 0.06862752514351911 2202 6 -0.021468132245253457 2202 7 0.0034484677052404322 2202 8 -0.01962917911860984 2202 9 0.016440483192411648 2202 10-11 0.02298424348300898 2202 12-13 0.022520501199927878 2202 14-15 0.008465463103547677 2202 16-17 -0.02324276961209648 2202 18-19 0.055795948369976145 2202 20-21 -2.070553211055426E-4 2202 22-23 -0.08104387541772695 2202 24-25 -0.007726335358896108 2202 26-27 -0.06663074914436606 2202 28-29 -0.06913469769892089 2202 30-31 -0.04580007368482342 2202 32-33 -0.05879178064758861 2202 34-35 -0.0749139567257231 2202 36-37 -0.0249656445850448 2202 38-39 -0.08118873762026624 2202 40-41 -0.11795310959621474 2202 42-43 -0.09555630232951984 2202 44-45 -0.1129746388350199 2202 46-47 -0.04788402796646807 2202 48-49 -0.10767098998464064 2202 50-51 -0.18476353001861412 2202 52-53 -0.1303428373019031 2202 54-55 -0.14332825814951633 2202 56-57 -0.1474225502729496 2202 58-59 -0.1728044025554425 2202 60-61 -0.14791611537857108 2202 62-63 -0.14189397873926168 2202 64-65 -0.15878279100777348 2202 66-67 -0.14098659441552286 2202 68-69 -0.17013343452317287 2202 70-71 -0.20155853662485868 2202 72-73 -0.19379581204864138 2202 74-75 -0.21340674793727032 2202 76-77 -0.19663011975116262 2202 78-79 -0.20336621513762765 2202 80-81 -0.15776677745035528 2202 82-83 -0.23568030915132 2202 84-85 -0.27792067712898216 2202 86-87 -0.23612129704859086 2202 88-89 -0.268860092328417 2202 90-91 -0.2977825218180925 2202 92-93 -0.3429461851191462 2202 94-95 -0.28060599429007027 2202 96-97 -0.2965804129723466 2202 98-99 -0.3164939686632309 2202 100-101 -0.3290391915204438 1102 1 0.01065006067084795 1102 2 0.0553172606084118 1102 3 -0.004888247162298853 1102 4 -0.0011837575518285348 1102 5 -0.03762338973493229 1102 6 -0.10038675415863452 1102 7 -0.11748350527776097 1102 8 -0.08786349424860163 1102 9 -0.04745253375729419 1102 10-11 -0.04541741079171402 1102 12-13 -0.04628629760475178 1102 14-15 -0.06317602901643227 1102 16-17 -0.07903861546775204 1102 18-19 -0.06161073499765024 1102 20-21 -0.09843768282447485 1102 22-23 -0.15813425270923176 1102 24-25 -0.10159908781155025 1102 26-27 -0.12126797839123782 1102 28-29 -0.16245735009901097 1102 30-31 -0.20102741043707084 1102 32-33 -0.1562382252384822 1102 34-35 -0.17595513929445872 1102 36-37 -0.20779957788474945 1102 38-39 -0.2186301296585924 1102 40-41 -0.22289902731405675 1102 42-43 -0.22696118337448468 1102 44-45 -0.28333007601943905 1102 46-47 -0.31358887746690556 1102 48-49 -0.291900259541805 1102 50-51 -0.2916711604005471 1102 52-53 -0.2892558327734065 1102 54-55 -0.33250767204179255 1102 56-57 -0.27966310916358594 1102 58-59 -0.2760965593303766 1102 60-61 -0.27432303121014456 1102 62-63 -0.34123180547082654 1102 64-65 -0.32544958369742716 1102 66-67 -0.34955900790509986 1102 68-69 -0.34303630046048994 1102 70-71 -0.4054551384439691 1102 72-73 -0.38155072990200267 1102 74-75 -0.3803591281141294 1102 76-77 -0.36629343866553654 1102 78-79 -0.4275783339350383 1102 80-81 -0.5093616759621042 1102 82-83 -0.47230863962393244 1102 84-85 -0.5059409683816938 1102 86-87 -0.5083409438778901 1102 88-89 -0.48757213153746903 1102 90-91 -0.46316698209749063 1102 92-93 -0.6941581288415506 1102 94-95 -0.6225209504854519 1102 96-97 -0.5894380986977552 1102 98-99 -0.5882873087940581 1102 100-101 -0.5924109773767441 2203 1 0.1008991731824338 2203 2 0.0737993823425569 2203 3 0.028304767656869956 2203 4 0.03831920058167526 2203 5 0.019012488529739358 2203 6 -0.1045144181619051 2203 7 -0.12443156335602623 2203 8 -0.09878696953333588 2203 9 -0.10043967394024378 2203 10-11 -0.04030351960268774 2203 12-13 -0.061360210778360624 2203 14-15 -0.06323668057254395 2203 16-17 -0.0778201870662798 2203 18-19 -0.03772239317991222 2203 20-21 -0.05176447010972396 2203 22-23 -0.13986145890287105 2203 24-25 -0.08513822700541596 2203 26-27 -0.11496489921253072 2203 28-29 -0.13658006655153798 2203 30-31 -0.15762308447880002 2203 32-33 -0.10594314800312077 2203 34-35 -0.138312375348022 2203 36-37 -0.16699839812387296 2203 38-39 -0.15963229560905745 2203 40-41 -0.17641233908943832 2203 42-43 -0.16077584977829673 2203 44-45 -0.16964688508132753 2203 46-47 -0.11766676506906038 2203 48-49 -0.1329810017109594 2203 50-51 -0.18633255003795313 2203 52-53 -0.1912296787778871 2203 54-55 -0.19223097403329348 2203 56-57 -0.2144732632915023 2203 58-59 -0.25112156170931144 2203 60-61 -0.19874473651503877 2203 62-63 -0.26335520742348706 2203 64-65 -0.2956842388080645 2203 66-67 -0.26974748659181813 2203 68-69 -0.3046554390998537 2203 70-71 -0.2540614610033174 2203 72-73 -0.3078609160804646 2203 74-75 -0.2908221260171686 2203 76-77 -0.33721248167982765 2203 78-79 -0.29424796386651764 2203 80-81 -0.3403856481027958 2203 82-83 -0.382517820328772 2203 84-85 -0.4464938571780195 2203 86-87 -0.44320231171124647 2203 88-89 -0.43016151937424496 2203 90-91 -0.5170586864013842 2203 92-93 -0.578779485657904 2203 94-95 -0.5352815908493938 2203 96-97 -0.5610326317616341 2203 98-99 -0.5275515051563673 2203 100-101 -0.5244517577035026 1103 1 0.07447421632620177 1103 2 0.03494565332236732 1103 3 0.08611631902616068 1103 4 0.06347366959108314 1103 5 0.06347204484869451 1103 6 -0.05409445223335041 1103 7 -0.06706226852280395 1103 8 -0.05909649118814286 1103 9 -0.020293914999761853 1103 10-11 0.002032124230410659 1103 12-13 -0.00488112830566223 1103 14-15 -0.04712806442026363 1103 16-17 0.009850840669088257 1103 18-19 0.007993874270148638 1103 20-21 0.006752919578666194 1103 22-23 -0.04907030007191793 1103 24-25 -0.029287279804812272 1103 26-27 -0.03995551316472046 1103 28-29 -0.0649467256236207 1103 30-31 -0.05747146537125758 1103 32-33 -0.04041288182892799 1103 34-35 -0.04360458667677847 1103 36-37 -0.0900170557081168 1103 38-39 -0.053385789965943786 1103 40-41 -0.02537393801584642 1103 42-43 -0.05075693566423212 1103 44-45 -0.0546592530009562 1103 46-47 -0.07024455528014784 1103 48-49 -0.10517045817786652 1103 50-51 -0.14760865503335907 1103 52-53 -0.1069377235873219 1103 54-55 -0.11241117213499052 1103 56-57 -0.0798255390395255 1103 58-59 -0.11673930010861966 1103 60-61 -0.11014968716843043 1103 62-63 -0.08718771360250344 1103 64-65 -0.14454820779025113 1103 66-67 -0.09310467160175762 1103 68-69 -0.179766543017287 1103 70-71 -0.10260273998568437 1103 72-73 -0.08610030272490832 1103 74-75 -0.08702905015377382 1103 76-77 -0.15842130549336275 1103 78-79 -0.1450122822439397 1103 80-81 -0.19098352767687032 1103 82-83 -0.2222743290011664 1103 84-85 -0.3350675357390003 1103 86-87 -0.16225000709782478 1103 88-89 -0.25575803003695086 1103 90-91 -0.23225572662177285 1103 92-93 -0.32437448193827123 1103 94-95 -0.1852287070182257 1103 96-97 -0.23295090928373696 1103 98-99 -0.26781217875640095 1103 100-101 -0.31066047067462677 2204 1 0.02343366287161075 2204 2 0.0824934237603614 2204 3 0.05956326309999582 2204 4 0.056538088099884476 2204 5 0.09162068379073673 2204 6 -0.020692206609908226 2204 7 0.04119268361775141 2204 8 0.06200247679701221 2204 9 0.05717790106034215 2204 10-11 0.0640456423838458 2204 12-13 0.05202592076186363 2204 14-15 0.03208425965576822 2204 16-17 0.06200483248288435 2204 18-19 0.08087782343918803 2204 20-21 0.0846490008106926 2204 22-23 8.783878288198821E-4 2204 24-25 0.038828526232634886 2204 26-27 0.05997349269403429 2204 28-29 -0.013991357092720591 2204 30-31 0.014039367194207841 2204 32-33 0.024913645963181352 2204 34-35 0.045319271277008966 2204 36-37 -0.028737113509151868 2204 38-39 0.04827039804995792 2204 40-41 0.08842934857339912 2204 42-43 0.07130104157700856 2204 44-45 0.06333333158605825 2204 46-47 0.07322445641583641 2204 48-49 0.051098719744373966 2204 50-51 0.05267030296293029 2204 52-53 0.041533765209806006 2204 54-55 0.017245260070986035 2204 56-57 0.02953857712817154 2204 58-59 0.04575028812134008 2204 60-61 0.0635902447681147 2204 62-63 0.04025381001484618 2204 64-65 0.048276315088692456 2204 66-67 0.06602644821089143 2204 68-69 0.04604284953072835 2204 70-71 0.08390286180305395 2204 72-73 0.04639695114246223 2204 74-75 0.06509206450351712 2204 76-77 0.07047797655965837 2204 78-79 0.10876019294833839 2204 80-81 0.12903063494526634 2204 82-83 -0.022644918619626253 2204 84-85 -0.021458786573504085 2204 86-87 -0.002899639193493897 2204 88-89 0.023639518447545527 2204 90-91 0.00444622289604979 2204 92-93 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0.42585335967506666 1214 90-91 0.5042221034992025 1214 92-93 0.5070876170804297 1214 94-95 0.4344278071308594 1214 96-97 0.4664149570384808 1214 98-99 0.5384030606199488 1214 100-101 0.6195824796434124 1215 1 -0.12494531727423208 1215 2 -0.05907008379536194 1215 3 -0.03622727751785959 1215 4 -0.03382687439052745 1215 5 -0.037023880908812146 1215 6 0.04292127608999863 1215 7 -0.03092054346349471 1215 8 0.016220510540989608 1215 9 -0.027584022701368838 1215 10-11 -0.03275033227612312 1215 12-13 -0.017671152681373314 1215 14-15 -0.0014814401784946085 1215 16-17 -0.005443446943388608 1215 18-19 0.03496540014049998 1215 20-21 -0.02859900271310778 1215 22-23 0.010360614270553015 1215 24-25 -0.05469700960752277 1215 26-27 0.01457418141428235 1215 28-29 0.038875538370206186 1215 30-31 0.03153981352236457 1215 32-33 0.008262359918035145 1215 34-35 -3.108940091749446E-4 1215 36-37 0.06010911689845955 1215 38-39 0.06927306937994615 1215 40-41 0.025547904805307553 1215 42-43 0.08573263257179775 1215 44-45 0.09487862145455495 1215 46-47 0.05428504515825949 1215 48-49 0.05751528319240862 1215 50-51 0.12890103433557698 1215 52-53 0.11796743802757703 1215 54-55 0.11358663171488814 1215 56-57 0.15539497985739104 1215 58-59 0.12487368434115353 1215 60-61 0.17149926018169026 1215 62-63 0.14933950281203323 1215 64-65 0.2084417925418265 1215 66-67 0.25048199996260223 1215 68-69 0.264488764967993 1215 70-71 0.1919011242443105 1215 72-73 0.2317110335053414 1215 74-75 0.16501386468661394 1215 76-77 0.273147832013656 1215 78-79 0.21773816572613214 1215 80-81 0.24737512761224423 1215 82-83 0.3493070584144107 1215 84-85 0.30104465726754626 1215 86-87 0.33528214017930935 1215 88-89 0.39480598976934544 1215 90-91 0.36966387208762796 1215 92-93 0.45631276902629736 1215 94-95 0.3758499183679689 1215 96-97 0.4765373866874043 1215 98-99 0.39336793045892904 1215 100-101 0.5173830454941921 1212 1 -0.10899198972643376 1212 2 -0.06743744904347437 1212 3 -0.11754995609334173 1212 4 -0.039461130770057196 1212 5 -0.03866878049824862 1212 6 -0.13097694043580788 1212 7 -0.09680603291693757 1212 8 -0.21717228070024674 1212 9 -0.12997447041935573 1212 10-11 -0.22636532244936802 1212 12-13 -0.22037150872363043 1212 14-15 -0.08443628226030597 1212 16-17 -0.040302071453687915 1212 18-19 -0.05086459608954641 1212 20-21 -0.041055180583008166 1212 22-23 0.02709519785233283 1212 24-25 0.023069101142354498 1212 26-27 0.01122438517482749 1212 28-29 0.05435280365382056 1212 30-31 -0.002516624892614061 1212 32-33 -0.019909025169511096 1212 34-35 -0.0377650612499707 1212 36-37 -0.06359731366428889 1212 38-39 -0.05554462553578787 1212 40-41 -0.16970496629470233 1212 42-43 -0.17965394102629517 1212 44-45 0.016535572435834922 1212 46-47 -0.027394665249026673 1212 48-49 0.04299902376511966 1212 50-51 -0.00186216745233736 1212 52-53 -0.05835476538462814 1212 54-55 -0.06330288532108597 1212 56-57 -0.07900444117098004 1212 58-59 0.08028771456982042 1212 60-61 -0.23050626259810514 1212 62-63 -0.1743430072808465 1212 64-65 -0.1268180059939752 1212 66-67 -0.08932167168960348 1212 68-69 0.1340057657535496 1212 70-71 0.005309923003537165 1212 72-73 0.17191476123409188 1212 74-75 0.10263731129722942 1212 76-77 0.12144449825348147 1212 78-79 0.18972819938604601 1212 80-81 0.23263908942355016 1212 82-83 0.1615064382311786 1212 84-85 0.22821504848587892 1212 86-87 0.19403758447880648 1212 88-89 0.24367498046487412 1212 90-91 0.22352415509411117 1212 92-93 0.3039262253847568 1212 94-95 0.1961142662844324 1212 96-97 0.2668125508649979 1212 98-99 0.2736167410535799 1212 100-101 0.3517792879236197 1213 1 -0.23404204494345038 1213 2 -0.17902365973180423 1213 3 -0.19822208962354182 1213 4 -0.1753088477039384 1213 5 -0.14294067295406165 1213 6 -0.051610927894813585 1213 7 -0.05602310048389114 1213 8 -0.10957777081002718 1213 9 -0.1100058044821921 1213 10-11 -0.11767062641352766 1213 12-13 -0.17449450774986985 1213 14-15 -0.18259509691478115 1213 16-17 -0.2606131192575418 1213 18-19 -0.29807935233925775 1213 20-21 -0.23400251947665396 1213 22-23 -0.2852833123457046 1213 24-25 -0.28834236249649337 1213 26-27 -0.1499575789818266 1213 28-29 -0.15841528211871747 1213 30-31 -0.15040059675828843 1213 32-33 -0.14005972785511744 1213 34-35 -0.14327090113643948 1213 36-37 -0.08351039409258476 1213 38-39 -0.03910552354983565 1213 40-41 -0.03322966155442941 1213 42-43 0.022656241327858595 1213 44-45 0.041861415423561255 1213 46-47 0.02700311100506525 1213 48-49 0.06225092770584695 1213 50-51 0.05279712915422152 1213 52-53 0.07448919768773976 1213 54-55 0.06700299311522429 1213 56-57 0.06810384297224203 1213 58-59 0.09476374097786078 1213 60-61 0.12605046273301923 1213 62-63 0.14987124873884738 1213 64-65 0.11816917017197426 1213 66-67 0.17869473057391616 1213 68-69 0.20323377272951149 1213 70-71 0.14920777240330096 1213 72-73 0.14497794451055057 1213 74-75 0.13247156011381378 1213 76-77 0.2244912318364669 1213 78-79 0.2842114809040055 1213 80-81 0.25077970142051953 1213 82-83 0.23685039977755906 1213 84-85 0.2704493476623142 1213 86-87 0.25539667848621406 1213 88-89 0.2688751725087073 1213 90-91 0.2908724901885762 1213 92-93 0.327409172790567 1213 94-95 0.2694813222843919 1213 96-97 0.32697899518585416 1213 98-99 0.3407184087409334 1213 100-101 0.10882069741523281 >>END_MODULE >>Per sequence quality scores pass #Quality Count 13 24.0 14 57.0 15 101.0 16 148.0 17 214.0 18 482.0 19 1178.0 20 2612.0 21 5383.0 22 10529.0 23 17846.0 24 27121.0 25 38887.0 26 53024.0 27 70717.0 28 90640.0 29 112424.0 30 138489.0 31 168356.0 32 203336.0 33 248859.0 34 309624.0 35 402417.0 36 566103.0 37 919966.0 38 2074827.0 39 8045647.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 18.085522918082166 30.16788785852948 30.654549503666466 21.092039719721893 2 20.109654434411944 31.173513863070678 30.196726859695737 18.520104842821635 3 20.254080776157483 30.137654044400435 29.993513218695284 19.614751960746794 4 20.31139807347851 29.42470770065995 30.094771556555845 20.169122669305693 5 20.69151911414298 29.66993114626821 30.00602725545348 19.632522484135333 6 20.551578498233514 29.88557045367718 29.279471309927867 20.28337973816144 7 20.68231345729158 30.095852316649975 29.533790445503378 19.688043780555066 8 20.148471499349693 30.11194767336418 29.613672080592256 20.12590874669387 9 19.997810269878684 29.808282391386648 30.0956270985839 20.098280240150768 10-11 20.097903540088907 30.03804645654667 29.827002139534862 20.037047863829557 12-13 19.89425273606444 30.054796053772144 30.024860459714198 20.026090750449217 14-15 19.88595717212872 29.940384011796617 30.13785665217752 20.035802163897145 16-17 19.955517901102283 29.835866310898705 30.143791401237934 20.064824386761078 18-19 19.93000819971203 29.72645813968173 30.15422076419954 20.1893128964067 20-21 20.011330226811065 29.691486347950185 30.09299654149009 20.20418688374866 22-23 20.079550758254598 29.6556440028618 30.028225658700485 20.236579580183115 24-25 20.10380853364108 29.654682191619592 29.958164942569788 20.28334433216954 26-27 20.191006168010837 29.61569800620628 29.880692766198997 20.312603059583882 28-29 20.217807465889948 29.682589027169413 29.818585446987694 20.28101805995294 30-31 20.283584400940665 29.56929942691798 29.865811781315642 20.281304390825714 32-33 20.33114539093575 29.630451461927176 29.76282952152503 20.275573625612047 34-35 20.344866323214276 29.607691053489038 29.755021357971295 20.29242126532539 36-37 20.355896313084994 29.57449413704442 29.767291788012678 20.302317761857903 38-39 20.363817724539427 29.565734992215337 29.74328938916298 20.327157894082255 40-41 20.40651001766691 29.59267563416993 29.6964272917386 20.304387056424556 42-43 20.40370665122537 29.533117010086666 29.705739525577012 20.357436813110947 44-45 20.39635803156678 29.47546769183878 29.711675709918694 20.416498566675738 46-47 20.41232883517411 29.547940587962195 29.688557882597532 20.351172694266157 48-49 20.419568848337317 29.462929811624715 29.70008321561729 20.417418124420674 50-51 20.375992613303133 29.504181594764283 29.68232302495661 20.43750276697598 52-53 20.41022533015515 29.510968474227134 29.702226707676417 20.376579487941303 54-55 20.40017402411366 29.460762526343583 29.708405962143424 20.430657487399337 56-57 20.435833318341817 29.465433675650694 29.68881094781835 20.40992205818914 58-59 20.422075832163006 29.468794194955418 29.70857476014818 20.400555212733394 60-61 20.414467574471175 29.454277204246388 29.639657946546656 20.491597274735785 62-63 20.399340502625236 29.454456354195024 29.69144309743909 20.454760045740645 64-65 20.43655625158346 29.470024996673466 29.663595149753334 20.429823601989742 66-67 20.42022154969304 29.481791651792 29.63548141078634 20.46250538772862 68-69 20.36943201165416 29.47259983294624 29.63761400734511 20.520354148054498 70-71 20.460355594961726 29.491394506281445 29.586869009640242 20.461380889116583 72-73 20.44131357636211 29.505322999267626 29.594176293211284 20.459187131158988 74-75 20.46391003199271 29.49400696154918 29.620602122212603 20.4214808842455 76-77 20.43675420621355 29.497657316357724 29.609455226316257 20.456133251112472 78-79 20.459078673187374 29.495549084479677 29.597390403670776 20.447981838662173 80-81 20.48596654527641 29.480821003212256 29.588666138930154 20.444546312581185 82-83 20.439411565357368 29.534904887931457 29.63131446436919 20.394369082341985 84-85 20.437654369113762 29.524222350607864 29.59632266199858 20.44180061827979 86-87 20.402776097527532 29.513127891829953 29.607396717423633 20.47669929321888 88-89 20.473131334131168 29.487017742748943 29.57177638053371 20.46807454258618 90-91 20.469330087894825 29.470458292339686 29.55562514296882 20.504586476796668 92-93 20.427119677343395 29.461550555434606 29.603863563275905 20.507466203946095 94-95 20.456591925797483 29.50690410222756 29.53064899105139 20.50585498092357 96-97 20.49519451359986 29.525366715979807 29.49645254472285 20.482986225697484 98-99 20.4824220454658 29.521305242046793 29.492353064367034 20.50391964812037 100-101 20.562720491926935 29.50964979045818 29.410467748977386 20.517161968637495 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 599.0 1 581.5 2 595.0 3 683.0 4 828.0 5 928.0 6 1098.5 7 1303.0 8 1460.0 9 1752.5 10 2119.5 11 2586.0 12 3146.5 13 3814.0 14 4854.0 15 6595.5 16 9373.5 17 13669.0 18 20258.0 19 29753.0 20 42615.5 21 58652.0 22 78395.5 23 101995.5 24 129084.0 25 159921.5 26 193709.5 27 229579.5 28 267093.5 29 305541.5 30 342357.0 31 376801.5 32 411064.5 33 443847.0 34 473818.5 35 501382.0 36 526766.5 37 545820.0 38 554421.5 39 550889.0 40 536549.5 41 515986.0 42 488638.5 43 457175.5 44 427966.5 45 403494.5 46 383064.0 47 368224.5 48 352848.5 49 332269.0 50 310829.0 51 287713.5 52 263703.5 53 241639.5 54 222284.5 55 204564.5 56 187459.5 57 171122.5 58 153923.5 59 135048.0 60 115916.5 61 97274.0 62 80393.5 63 66198.5 64 54423.5 65 44924.5 66 37110.0 67 30494.0 68 24856.0 69 20036.0 70 16024.0 71 12711.0 72 10196.5 73 8394.0 74 7006.5 75 5973.0 76 5175.5 77 4583.0 78 4097.5 79 3591.0 80 3095.5 81 2638.5 82 2303.0 83 1969.0 84 1599.0 85 1244.5 86 946.0 87 668.0 88 446.0 89 301.5 90 196.5 91 121.0 92 65.5 93 31.5 94 15.0 95 11.5 96 8.0 97 7.5 98 5.5 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.08240425594442109 2 1.9986659275057222E-4 3 0.0 4 0.0 5 0.0027759248993135025 6 0.0 7 0.0 8 1.5545179436155616E-4 9 0.0036790257998901617 10-11 0.0 12-13 6.662219758352406E-5 14-15 3.7752578630663637E-4 16-17 0.0015249080780228841 18-19 0.0 20-21 1.0363452957437077E-4 22-23 1.9246412635240284E-4 24-25 4.4414798389016044E-5 26-27 0.0032718901479908483 28-29 0.0014767920464347833 30-31 0.002213337453052633 32-33 0.002609369405354693 34-35 0.004356351475322657 36-37 0.005973790383322658 38-39 0.007683760121299776 40-41 0.009438144657665909 42-43 0.017558650296457676 44-45 0.010552215850590393 46-47 0.013191195121537764 48-49 0.014294162614864997 50-51 0.010992662601281471 52-53 0.015852381791679644 54-55 0.012491662046910764 56-57 0.010141378965491997 58-59 0.007665253955304352 60-61 0.004411869973308927 62-63 0.0017654882359633877 64-65 0.0015804265760091542 66-67 0.002246648551844395 68-69 0.010241312261867282 70-71 0.005126207980732268 72-73 0.0016766586391853556 74-75 0.021952014103771178 76-77 0.03503957469573457 78-79 0.0381967266145538 80-81 0.02616031625113045 82-83 0.04591749906784442 84-85 0.020934174974022897 86-87 0.01795098101556065 88-89 0.01793617608276431 90-91 0.0060774249128970285 92-93 0.01862090422459498 94-95 0.021811367242205962 96-97 0.013276323485116712 98-99 0.0039085022582334115 100-101 9.623206317620142E-5 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1.3509011E7 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 95.41199074085856 #Duplication Level Percentage of deduplicated Percentage of total 1 98.12226630577105 93.62040764238286 2 1.2838802633601192 2.4499514360017347 3 0.20093873157304376 0.5751589318898134 4 0.09176007065954102 0.3502004404859462 5 0.0786747835603974 0.3753258860301844 6 0.04809327268870414 0.2753204933083335 7 0.03561205566224234 0.23784719875761637 8 0.013124115736311883 0.10017584072919564 9 0.016045334806645273 0.13778256024050528 >10 0.10915056197595954 1.796869951833806 >50 0.0 0.0 >100 4.455632555177599E-4 0.05183567597969504 >500 0.0 0.0 >1k 8.940950463578108E-6 0.029123942360341597 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 8.142713037986274E-5 0.0 7.402466398169341E-6 2 8.142713037986274E-5 0.0 1.4804932796338681E-5 3 8.882959677803209E-5 0.0 2.9609865592677363E-5 4 9.623206317620142E-5 0.0 3.70123319908467E-5 5 1.1843946237070945E-4 0.0 3.70123319908467E-5 6 1.258419287688788E-4 0.0 3.70123319908467E-5 7 1.258419287688788E-4 0.0 3.70123319908467E-5 8 1.4064686156521747E-4 0.0 5.9219731185354725E-5 9 1.480493279633868E-4 0.0 8.142713037986274E-5 10-11 1.7395796035697952E-4 0.0 8.882959677803209E-5 12-13 2.0356782594965685E-4 0.0 1.221406955697941E-4 14-15 2.368789247414189E-4 0.0 1.2954316196796346E-4 16-17 2.7759248993135027E-4 0.0 1.6285426075972548E-4 18-19 3.331109879176203E-4 0.0 1.9616535955148753E-4 20-21 3.812270195057211E-4 3.7012331990846703E-6 2.1837275874599555E-4 22-23 4.1083688509839836E-4 7.402466398169341E-6 2.220739919450802E-4 24-25 4.478492170892451E-4 7.402466398169341E-6 2.4058015794050356E-4 26-27 5.181726478718538E-4 7.402466398169341E-6 2.516838575377576E-4 28-29 6.1810594424714E-4 7.402466398169341E-6 2.8129372313043493E-4 30-31 6.773256754324947E-4 1.1103699597254011E-5 3.0350112232494295E-4 32-33 7.40246639816934E-4 1.4804932796338681E-5 3.331109879176203E-4 34-35 8.327774697940508E-4 2.5908632393592692E-5 3.7752578630663637E-4 36-37 9.179058333729982E-4 3.70123319908467E-5 4.1823935149656774E-4 38-39 0.0010289428293455383 4.4414798389016044E-5 4.441479838901604E-4 40-41 0.0011510835249153323 4.4414798389016044E-5 4.478492170892451E-4 42-43 0.0013213402520732271 4.4414798389016044E-5 4.737578494828378E-4 44-45 0.001436078481244852 4.811603158810072E-5 4.811603158810071E-4 46-47 0.0015767253428100696 5.1817264787185385E-5 4.811603158810071E-4 48-49 0.0017543845363661337 5.1817264787185385E-5 5.144714146727692E-4 50-51 0.0019061350975286052 5.9219731185354725E-5 5.366788138672772E-4 52-53 0.0021060016902791773 5.9219731185354725E-5 5.662886794599545E-4 54-55 0.002357685547816935 6.662219758352406E-5 5.773923790572085E-4 56-57 0.0027204064013272326 6.662219758352406E-5 5.921973118535473E-4 58-59 0.0031127371204302074 6.662219758352406E-5 6.144047110480553E-4 60-61 0.0035531838711212833 7.032343078260873E-5 6.366121102425633E-4 62-63 0.004263820645345541 8.882959677803209E-5 6.699232090343253E-4 64-65 0.005248348676302063 8.882959677803209E-5 6.884293750297487E-4 66-67 0.006603000027167052 8.882959677803209E-5 7.217404738215107E-4 68-69 0.008150115504384443 8.882959677803209E-5 7.587528058123574E-4 70-71 0.010567020783386734 9.99332963752861E-5 7.994663710022888E-4 72-73 0.014231241650480557 1.0733576277345544E-4 8.253750033958815E-4 74-75 0.019324138532421063 1.110369959725401E-4 8.475824025903895E-4 76-77 0.02608999282034784 1.110369959725401E-4 8.660885685858129E-4 78-79 0.035150611691707113 1.110369959725401E-4 8.808935013821515E-4 80-81 0.048456545042416505 1.110369959725401E-4 9.179058333729982E-4 82-83 0.06589675587650347 1.110369959725401E-4 9.512169321647603E-4 84-85 0.08883329801123117 1.1473822917162478E-4 9.99332963752861E-4 86-87 0.11949801506564767 1.1843946237070945E-4 0.0010030341969519457 88-89 0.15950094348135477 1.1843946237070945E-4 0.001032644062544623 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATGCCG 1340 0.0 16.662266 46-47 TCTCGTA 1285 0.0 15.52716 40-41 CTCGTAT 1395 0.0 14.983549 42-43 ATGCCGT 1620 0.0 14.075611 46-47 CGTATGC 1800 0.0 13.194957 44-45 GCCGTCT 1925 0.0 12.0918255 48-49 TGCCGTC 1805 0.0 11.974593 48-49 TCGTATG 2165 0.0 11.409896 42-43 ATCTCGT 1975 0.0 10.824087 40-41 CGTCTGA 2335 0.0 10.474391 16-17 AGATCGG 6535 0.0 9.449645 94-95 GATCGGA 6065 0.0 9.281224 94-95 ACACGTC 2660 0.0 8.837699 12-13 CGTCTTC 2770 0.0 8.831472 50-51 ACGTCTG 2980 0.0 8.526154 14-15 CGGAAGA 5690 0.0 8.513473 4 TAGATCG 2395 0.0 8.429486 94-95 TCGGAAG 5650 0.0 8.23752 3 ATCGGAA 5945 0.0 8.030303 94-95 CCGTCTT 2915 0.0 7.9847846 50-51 >>END_MODULE