FastQCFastQC Report
Fri 3 Apr 2015
H2K5YBCXX l01n01 10551-bar6.34100000004444.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2K5YBCXX l01n01 10551-bar6.34100000004444.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13509011
Sequences flagged as poor quality0
Sequence length101
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG13400.016.66226646-47
TCTCGTA12850.015.5271640-41
CTCGTAT13950.014.98354942-43
ATGCCGT16200.014.07561146-47
CGTATGC18000.013.19495744-45
GCCGTCT19250.012.091825548-49
TGCCGTC18050.011.97459348-49
TCGTATG21650.011.40989642-43
ATCTCGT19750.010.82408740-41
CGTCTGA23350.010.47439116-17
AGATCGG65350.09.44964594-95
GATCGGA60650.09.28122494-95
ACACGTC26600.08.83769912-13
CGTCTTC27700.08.83147250-51
ACGTCTG29800.08.52615414-15
CGGAAGA56900.08.5134734
TAGATCG23950.08.42948694-95
TCGGAAG56500.08.237523
ATCGGAA59450.08.03030394-95
CCGTCTT29150.07.984784650-51
Produced by FastQC (version 0.11.2)