FastQCFastQC Report
Mon 27 Jul 2020
H2K2WBGXG_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2K2WBGXG_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17707479
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCA14579198.233351568566027No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACTCCGCCAGTAGGTCC11127716.284186472845739No Hit
GATAGGTCGCATCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCACTA10961766.190469010297852No Hit
ATACTGTATCTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCACTAT10704806.045355185794658No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTC9692535.4736927825807395No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAATCACCGCCAGTAGGT8520194.811633547610024No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCACTATG8100814.574795768499852No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACACCGCCTGTAGGTCCACTATG4259592.405531583575505No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCC3564462.0129686444919686No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGCCAGTAGGT3024731.7081652334586985No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACACCGCCACTAGGTCCACTATG735610.4154233360943136No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACTCCGCCAGTAGGTC556300.31416103895986547No Hit
ATACTGTATCTCTTGTGGAAAGGACGAAACACCGCGAGTAGGTCCACTAT487410.2752565737900918No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACAGCGCCAGTAGGTCCA275990.1558606959240217No Hit
GATAGGTCGCATCTTGTGGAAAGGACGAAACACCGGCAGTAGGTCCACTA221660.1251787450940927No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGATT1593350.0142.809711
TCGATTC1611850.0141.527112
CGATTCC1618900.0140.965653
AACAATG1493250.0139.366172
GATAACG1635700.0139.281315
TCGCATC1288650.0139.240177
ACGATCG1274450.0139.197141
CGCATCT1289400.0139.19518
ATTCCTT1644550.0139.109535
GATTCCT1643900.0139.0124
CGATAGG1284450.0138.654196
ACTGTAT1294350.0138.508933
ACAATGG1499400.0138.44043
CCTTGGT1671500.0138.389458
TCCTTGG1675150.0138.12977
CAATGGT1506000.0137.832464
AACGCAT1682100.0137.585838
TAACGCA1682900.0137.557747
GCATCTT1304550.0137.553129
ACGCATT1682450.0137.496959
Produced by FastQC (version 0.11.5)