FastQCFastQC Report
Mon 27 Jul 2020
H2K2WBGXG_n01_0722_plasmid_20200710.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2K2WBGXG_n01_0722_plasmid_20200710.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9864423
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACTCCGCCAGTAGGTCC701398271.10382431896929No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCC225458822.85575142104105No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACTCCGCCAGTAGGTCA140570.14250199935667804No Hit
GATCGATAACGCATTTCTTGTGGAAAGGAAGAAAAACCGCCAGTAGGTCC130820.13261799499068522No Hit
GATCGATAACGCATTTCTTGTGGAAAGGAAGAAACTCCGCCAGTAGGTCC128800.130570232034859No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACTCCCCCAGTAGGTCC111830.11336699571784382No Hit
GATCGATAACGCATTTCTTGTGGAAGGACGAAACACCGCCAGTAGGTCCA98840.10019846067022876No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATAAC9815600.0144.859394
ATAACGC9817650.0144.852756
GATAACG9809300.0144.832675
GATCGAT9804300.0144.798651
ATCGATA9823300.0144.761372
ACGCATT9816050.0144.751659
AACGCAT9821550.0144.742148
TAACGCA9822700.0144.734797
TCGATAA9825600.0144.723793
CGTCTGA9519250.0134.27573145
GTCTGAA732050.0118.06063145
CTCTGAA32800.099.46536145
CGTCTAA44700.089.36701145
CGTTGAA28950.079.13556145
CGACTGA30300.078.2417145
CGCTGAA23700.078.00546145
CGTCAGA24450.077.6883145
CGTCGAA35350.076.08826145
TCTGAAC124050.070.48289145
AATAACG22350.066.184165