FastQCFastQC Report
Mon 27 Jul 2020
H2K2WBGXG_n01_0722_plasmid_20200612.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2K2WBGXG_n01_0722_plasmid_20200612.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7771896
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCA718698092.47396002211043No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACAGCGCCAGTAGGTCCA1426391.8353179198486442No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGAAGAAAAACCGCCAGTAGGTCCA154610.1989347258378136No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCAC116470.14986047162751534No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGAAGAAACACCGCCAGTAGGTCCA101210.13022562319413436No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACACCGCAGTAGGTCCAC90240.11611066334392534No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACACCACCAGTAGGTCCA88710.11414203175132555No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACACCCCCAGTAGGTCCA88420.11376889242985237No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACAAAACACCGCCAGTAGGTCCA78790.10137809358231246No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATTCC7730950.0144.875613
ATCGATT7734450.0144.84951
ATTCCTT7741900.0144.844885
TCGATTC7736900.0144.826072
GATTCCT7739000.0144.820474
TTCCTTG7737500.0144.786686
TCCTTGG7728300.0144.760187
CCTTGGT7729150.0144.718938
CTTGGTT7732950.0144.60299
TCTGAAC7504350.0133.87492145
CTGAACT579950.0122.00932145
TCAGAAC33850.097.451035145
TCTGAAA26100.084.44361145
TCTAACT30350.077.157394145
TGAACTC92950.075.26822145
TCGAACT24000.070.6868145
CCTTAGT14250.069.707798
TCTGAAT25000.067.569336145
CCTTGAT15450.063.8242958
CAATTCC14250.061.0594943