FastQCFastQC Report
Mon 27 Jul 2020
H2K2WBGXG_n01_0722_old_CD4_flowthrough.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2K2WBGXG_n01_0722_old_CD4_flowthrough.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8965408
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTC782707487.3030429847699No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACTCCGCCAGTAGGTC4393154.900111629052465No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAAGACCGCCAGTAGGTC1144821.2769301742876622No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGAAGAAACACCGCCAGTAGGTC201850.22514312789780455No Hit
CGATCGATACAGGTATTCTTGTGGAAAGAACGAAACACCGCCAGTAGGTC149270.16649549022197316No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACAAAACACCGCCAGTAGGTC146610.16352853099379303No Hit
CGATCGATACAGGTATTCTTGTGGAAAAGACGAAACACCGCCAGTAGGTC143830.16042772398088295No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCACCAGTAGGTC143820.16041656999882214No Hit
CGATCGATACAGGTATTCTTGTAGAAAGGACGAAACACCGCCAGTAGGTC133500.1489056605120481No Hit
CGATCGATACAGGTATTCTTGTGAAAAGGACGAAACACCGCCAGTAGGTC128340.14315020576866105No Hit
CGATCGATACAGGTATTCTTATGGAAAGGACGAAACACCGCCAGTAGGTC124530.13890053860348575No Hit
CGATCGATACAGATATTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTC114090.12725578133198176No Hit
CGATCGATACAGGTATTCTTGTCGAAAGGACGAAACACCGCCAGTAGGTC110480.12322919380802302No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGCAGTAGGTCC106330.11860029125277957No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCCCCAGTAGGTC102080.11385984887692785No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCTCCAGTAGGTC92830.10354241547066234No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACAGG8901050.0144.83717
ATCGATA8922800.0144.835893
TCGATAC8919950.0144.82774
CGATCGA8912700.0144.818661
CGATACA8919900.0144.795155
TACAGGT8901000.0144.786598
ACAGGTA8901550.0144.75829
GATACAG8919450.0144.680516
GATCGAT8925100.0144.64342
CACGTCT8657800.0135.5939145
ACGTCTG565650.0120.607124145
CACGTCG35750.0105.45287145
CACGCTG17450.087.2479145
ATACAGA21600.086.9411857
CACTCTG24200.083.583374145
CACGTTG22900.078.83063145
TACAGAT24300.076.683868
CGTCTGA93500.075.9881145
ACAGATA24350.075.925779
CACGACT24750.072.05946145
Produced by FastQC (version 0.11.5)