FastQCFastQC Report
Mon 27 Jul 2020
H2K2WBGXG_n01_0722_old_CD4_IL2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2K2WBGXG_n01_0722_old_CD4_IL2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8030426
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAATCACCGCCAGTAGGT538644667.0754702178938No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGCCAGTAGGT192083523.91946579172761No Hit
ACGATCTATAGGTAAGGTCTTGTGGAAAGGACGAATCACCGCCAGTAGGT970481.2085037580820743No Hit
ACGATCTATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGCCAGTAGGT821451.0229220716310692No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGAACGAATCACCGCCAGTAGGT112640.14026653131477707No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAAGACGAATCACCGCCAGTAGGT111910.13935748863136277No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGAAGAAACACCGCCAGTAGGT107410.133753800856891No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACAAATCACCGCCAGTAGGT107170.1334549375089192No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAATCACCACCAGTAGGT102240.12731578623599796No Hit
ACGATCGATAGGTAAGGTCTTGTGAAAAGGACGAATCACCGCCAGTAGGT101350.12620750132060243No Hit
ACGATCGATAGGTAAGGTCTTGTAGAAAGGACGAATCACCGCCAGTAGGT100320.12492487945222333No Hit
ACGATCGATAGGTAAGATCTTGTGGAAAGGACGAATCACCGCCAGTAGGT98350.12247170947095459No Hit
ACGATCGATAGGTAAGGTCTTATGGAAAGGACGAATCACCGCCAGTAGGT98280.1223845409944628No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAATCACCTCCAGTAGGT89570.11153829199098528No Hit
ACGATCGATAGATAAGGTCTTGTGGAAAGGACGAATCACCGCCAGTAGGT89300.11120207072451697No Hit
ACGATCGATAGGTAAAGTCTTGTGGAAAGGACGAATCACCGCCAGTAGGT85640.10664440466794663No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGATCG7782750.0144.839431
CGATAGG7779700.0144.817436
ATAGGTA7977400.0144.799778
GATAGGT7779000.0144.796987
GATCGAT7798500.0144.776123
ATCGATA7809650.0144.747634
TAGGTAA7976950.0144.706249
TCGATAG7801900.0144.657245
CGATCGA7818900.0144.34472
CTATAGG201750.0139.62956
CGATCTA207100.0137.768782
GATCTAT206650.0137.720493
ACGATCT207500.0137.492941
ATCTATA206850.0137.413774
TATAGGT207850.0135.319817
CACACGT7761300.0133.55052145
TCTATAG212350.0133.307485
ACACGTC615850.0120.441246145
CACACTC27200.088.75798145
CACACGC21000.085.61789145
Produced by FastQC (version 0.11.5)