FastQCFastQC Report
Mon 27 Jul 2020
H2K2WBGXG_n01_0722_CD8_prestim.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2K2WBGXG_n01_0722_CD8_prestim.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10137240
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAACAATGGTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCACTATG580907257.304276114603184No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACACCGCCTGTAGGTCCACTATG299381729.532861015424317No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACACCGCCACTAGGTCCACTATG5285515.213953699429036No Hit
TAACAATGGTCTTGTGGAAAGGAAGAAACACCGCCAGTAGGTCCACTATG473050.46664575367654315No Hit
TAACAATGGTCTTTTGGAAAGGACGAAACACCGCCAGTAGGTCCACTATG410840.4052779652055195No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACACCGCCTCTAGGTCCACTATG377660.37254716273857574No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACACCGCCAGGAGGTCCACTATG213220.2103333846293468No Hit
TAACAATGGTCTTGTGGAAAGGAAGAAACACCGCCTGTAGGTCCACTATG202380.1996401387359873No Hit
TAACAATGGTCTTTTGGAAAGGACGAAACACCGCCTGTAGGTCCACTATG166180.16393022163823684No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACACCGCCTCTACGTCCACTATG110940.10943807190122755No Hit
TAACAATGGTCTTGTGGAAAGGAAGAAAAAAAGAAACTAGGTCCACTATG108120.10665624963007683No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACACCACCAGTAGGTCCACTATG107320.10586708019145251No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACAATG10090700.0144.895812
ACAATGG10075350.0144.883383
AATGGTC10080150.0144.836675
CAATGGT10078850.0144.812914
GGTCTTG9984900.0144.795158
TAACAAT10104150.0144.754641
ATGGTCT10085300.0144.75126
TGGTCTT10082700.0144.686467
GTCTTGT10011300.0144.443889
CTGAACT9741750.0131.44589145
GGTCTTT93450.0123.441528
TGAACTC928800.0123.352844145
CTGAACA66100.0119.66169145
CGAACTC29950.0103.36247145
GTCTTTT111350.099.554259
CTAACTC33400.096.59293145
CAATGAT21650.088.419174
CTGAACC32600.087.84385145
CTGACTC19200.086.47012145
GAACTCC139350.086.05187145
Produced by FastQC (version 0.11.5)