FastQCFastQC Report
Mon 27 Jul 2020
H2K2WBGXG_n01_0722_CD8_presort_d3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2K2WBGXG_n01_0722_CD8_presort_d3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11231848
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCA1032579491.93317074803718No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACAGCGCCAGTAGGTCCA2039551.8158632488616298No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACACCACCAGTAGGTCCA225560.20082180599310107No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGAAGAAAAACCGCCAGTAGGTCCA224410.19979793173839247No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACACCTCCAGTAGGTCCA204870.18240097266273544No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCAC163700.14574627434416848No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGAAGAAACACCGCCAGTAGGTCCA145260.1293286732512762No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACACCGCAGTAGGTCCAC140990.1255269836272713No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACAAAACACCGCCAGTAGGTCCA135860.1209596141258322No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACACCCCCAGTAGGTCCA129740.11551082243990482No Hit
ATCGATTCCTTGGTTCTTGTGGAAAAGACGAAACACCGCCAGTAGGTCCA124780.11109480826307477No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGAACGAAACACCGCCAGTAGGTCCA120350.10715066656884957No Hit
ATCGATTCCTTGGTTCTTATGGAAAGGACGAAACACCGCCAGTAGGTCCA113570.10111426009326338No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATTCC11168800.0144.925893
TCGATTC11176150.0144.87992
ATTCCTT11186950.0144.865755
GATTCCT11181000.0144.855944
TTCCTTG11178700.0144.842276
ATCGATT11178450.0144.828121
CCTTGGT11166850.0144.791538
TCCTTGG11169850.0144.760367
CTTGGTT11174250.0144.63159
GCACACG10832200.0134.03444145
CACACGT828000.0116.88285145
GCACACC38300.099.18931145
CTGTATC37300.088.43719145
GAACACG27950.077.55713145
CCTTAGT18400.069.355888
GCACACT34300.068.694435145
CCTTGAT21350.068.602818
GCACCGT19350.064.81828145
CTATTCC24000.062.542413
AATTCCT21150.062.057374
Produced by FastQC (version 0.11.5)