FastQCFastQC Report
Mon 27 Jul 2020
H2K2WBGXG_n01_0722_CD8_presort_d2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2K2WBGXG_n01_0722_CD8_presort_d2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6940397
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATAGGTCGCATCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCACTA625659190.14745121928904No Hit
GATAGGTCGCATCTTGTGGAAAGGACGAAACACCGGCAGTAGGTCCACTA1353351.9499604993777735No Hit
GATAGGTCGCATCTTGTGGAAAGGAAGAAACACCGCCAGTAGGTCCACTA301930.43503275100833566No Hit
GATAGGTCGCATCTTGAGGAAAGGACGAAACACCGCCAGTAGGTCCACTA236470.3407153798262549No Hit
GATAGGTCGCATCTTGTGGAAAGGACGAAACACCGCCAGGAGGTCCACTA144130.20766823569314552No Hit
GATAGGTCGCATCTTGTGGAAAGGACGAAACACCACCAGTAGGTCCACTA139920.20160230027187206No Hit
GATAGGTCGCATCTTGTGGAAAGAACGAAACACCGCCAGTAGGTCCACTA126290.18196365424052832No Hit
GATAGGTCGCATCTTGTGGAAAAGACGAAACACCGCCAGTAGGTCCACTA124890.17994647856599558No Hit
GATAGGTCGCATCTTGTGGAAAGGACAAAACACCGCCAGTAGGTCCACTA124800.1798168029869185No Hit
GATAGGTCGCATCTTGTGAAAAGGACGAAACACCGCCAGTAGGTCCACTA110950.1598611722067196No Hit
GATAGGTCGCATCTTGTAGAAAGGACGAAACACCGCCAGTAGGTCCACTA109300.15748378659030599No Hit
GATAGGTCGCATCTTATGGAAAGGACGAAACACCGCCAGTAGGTCCACTA107810.15533693533669615No Hit
GATAGGTCGCATCTTGTGGAAAGGAAGAAAAAAAGCCAGTAGGTCCACTA103460.14906928234796943No Hit
GATAGGTCGCATCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCACTAT100100.14422806072909086No Hit
GATAGGTCGCATCTTGTGGAAAGGACGAAACACCTCCAGTAGGTCCACTA95950.1382485756938688No Hit
GATAAGTCGCATCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCACTA86130.12409952917678917No Hit
GATAGGTCGCATCTTGTGGAAAGGACGAAACACCGCCACTAGGTCCACTA83750.12067033053008351No Hit
GATAGATCGCATCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCACTA82860.11938798313698769No Hit
GATAGGTCGCATCTTGTGGAAAGGACGAAACACCGCAGTAGGTCCACTAT82060.11823531132296898No Hit
GATAGGTCACATCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCACTA80350.115771475320504No Hit
AATAGGTCGCATCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCACTA72100.10388454723843606No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATCT6895450.0144.881358
AGGTCGC6888600.0144.865424
GGTCGCA6889100.0144.842255
TAGGTCG6887850.0144.835943
GCATCTT6893750.0144.80679
TCGCATC6901800.0144.774297
GATAGGT6894800.0144.768891
ATAGGTC6904600.0144.709322
GTCGCAT6901000.0144.676536
ACGTCTG6662800.0132.61757145
CGTCTGA574300.0120.84864145
ACGACTG24550.0101.58731145
AAGTCGC15450.089.167734
ACGTCTA26950.086.89129145
ACGTCGA22400.084.47438145
ACGCTGA13950.083.15307145
AGATCGC16750.078.7841954
ATCGCAT16900.078.0866246
CGCATCC9400.077.9063648
GTCTGAA105750.075.00141145
Produced by FastQC (version 0.11.5)