FastQCFastQC Report
Mon 27 Jul 2020
H2K2WBGXG_n01_0722_CD8_presort_d1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2K2WBGXG_n01_0722_CD8_presort_d1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7638374
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACTGTATCTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCACTAT668969087.58002684864607No Hit
ATACTGTATCTCTTGTGGAAAGGACGAAACACCGCGAGTAGGTCCACTAT3180514.163857386401871No Hit
ATACTGTATCTCTTGTGGAAAGGACGAAACACCGCCACTAGGTCCACTAT1028581.3465954927056465No Hit
ATACTGTATCTCTTGTGGAAAGGAAGAAACACCGCCAGTAGGTCCACTAT343100.44917936723182184No Hit
ATACTGTATCTCTTTTGGAAAGGACGAAACACCGCCAGTAGGTCCACTAT242150.31701773178427767No Hit
ATACTGTATCTCTTGTGGAAAGGACGAAACACCGCCAGGAGGTCCACTAT152280.1993618013467264No Hit
ATACTGTATCTCTTGTGGAAAGGACGAAACACCACCAGTAGGTCCACTAT131240.1718166719775701No Hit
ATACTGTATCTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCACTATG110100.14414062469316113No Hit
ATACTGTATCTCTTGTGGAAAGGACGAAACACCGCCTGTAGGTCCACTAT89930.11773448118670284No Hit
ATACTGTATCTCTTGTGGAAAGAACGAAACACCGCCAGTAGGTCCACTAT89300.11690969832061117No Hit
ATACTGTATCTCTTGTGGAAAGGACAAAACACCGCCAGTAGGTCCACTAT87350.1143567989731846No Hit
ATACTGTATCTCTTGTGGAAAGGAAGAAAAACCGCCAGTAGGTCCACTAT84730.11092674959356534No Hit
ATACTGTATCTCTTGTGGAAAAGACGAAACACCGCCAGTAGGTCCACTAT82960.10860950249359352No Hit
ATACTGTATCTCTTGTGAAAAGGACGAAACACCGCCAGTAGGTCCACTAT76590.10027003129200011No Hit
ATACTGTATCTCTTGTAGAAAGGACGAAACACCGCCAGTAGGTCCACTAT76490.10013911337674747No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACTGT7598750.0144.879441
ACTGTAT7597600.0144.877463
CTGTATC7598950.0144.858374
TACTGTA7600650.0144.814562
ATCTCTT7606900.0144.807138
TGTATCT7605200.0144.786065
TATCTCT7615250.0144.751147
GTATCTC7609350.0144.728046
TCTCTTG7568500.0144.70569
GTCTGAA7355750.0131.1096145
TCTGAAC714750.0122.733376145
TCTCTTT55050.098.249439
GACTGAA20600.095.726715145
GCTGAAC18500.090.52567145
CTGAACT110050.085.97099145
GTCTAAC36400.082.45756145
ATATCTC13000.076.973486
ACTATAT13450.074.935323
GTCGAAC31350.071.689514145
GTCAGAA30500.065.84328145
Produced by FastQC (version 0.11.5)