FastQCFastQC Report
Mon 27 Jul 2020
H2K2WBGXG_n01_0722_CD8_low_d3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2K2WBGXG_n01_0722_CD8_low_d3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11338420
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATAGGTCGCATCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCACTA1018820389.85557952518958No Hit
GATAGGTCGCATCTTGTGGAAAGGACGAAACACCGGCAGTAGGTCCACTA2053761.8113282097505647No Hit
GATAGGTCGCATCTTGTGGAAAGGAAGAAACACCGCCAGTAGGTCCACTA492860.43468137535917706No Hit
GATAGGTCGCATCTTGAGGAAAGGACGAAACACCGCCAGTAGGTCCACTA389720.3437163202633171No Hit
GATAGGTCGCATCTTGTGGAAAGGACGAAACACCACCAGTAGGTCCACTA260500.22974982404955893No Hit
GATAGGTCGCATCTTGTGGAAAAGACGAAACACCGCCAGTAGGTCCACTA243700.21493294480183306No Hit
GATAGGTCGCATCTTGTGGAAAGGACGAAACACCTCCAGTAGGTCCACTA240980.2125340214950584No Hit
GATAGGTCGCATCTTGTGGAAAGGACGAAACACCGCCAGGAGGTCCACTA232090.2046934228931368No Hit
GATAGGTCGCATCTTGTGGAAAGGACAAAACACCGCCAGTAGGTCCACTA225650.19901361918150856No Hit
GATAGGTCGCATCTTGTGGAAAGAACGAAACACCGCCAGTAGGTCCACTA223290.19693220043004228No Hit
GATAGGTCGCATCTTATGGAAAGGACGAAACACCGCCAGTAGGTCCACTA196850.17361325475683562No Hit
GATAGGTCGCATCTTGTAGAAAGGACGAAACACCGCCAGTAGGTCCACTA195440.1723696952485443No Hit
GATAGGTCGCATCTTGTGAAAAGGACGAAACACCGCCAGTAGGTCCACTA189710.1673160810765521No Hit
GATAGGTCGCATCTTGTGGAAAGGAAGAAAAAAAGCCAGTAGGTCCACTA170410.15029430908362892No Hit
GATAGGTCGCATCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCACTAT161130.14210974721345654No Hit
GATAGATCGCATCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCACTA154670.13641230435986673No Hit
GATAAGTCGCATCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCACTA151950.13401338105309205No Hit
GATAGGTCACATCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCACTA137880.12160424468312164No Hit
GATAGGTCGCATCTTGTGGAAAGGACGAAACACCGCAGTAGGTCCACTAT137490.12126028141487086No Hit
GATAGGTCGCATCTTGTGGAAAGGACGAAACACCGCCACTAGGTCCACTA135640.11962866078342486No Hit
AATAGGTCGCATCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCACTA126440.11151465548109878No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGTCG11237500.0144.914473
AGGTCGC11243300.0144.880334
CGCATCT11262450.0144.874188
GGTCGCA11241950.0144.862235
GCATCTT11262050.0144.790579
GATAGGT11252900.0144.77041
TCGCATC11270900.0144.768777
ATAGGTC11269750.0144.754552
GTCGCAT11264200.0144.684236
TCTGAAC10925950.0128.83653145
TCGTATG75300.0110.240776145
TAAGTCG24100.099.589063
ATGATCT321900.095.83195145
GATAGAT27150.094.2673951
AAGTCGC25600.093.4717564
TCACATC25150.092.25747
TAGATCG28000.090.379123
CTGAACT1220200.089.76514145
ACTGAAC21100.086.93002145
GATAAGT27950.086.121761
Produced by FastQC (version 0.11.5)