FastQCFastQC Report
Mon 27 Jul 2020
H2K2WBGXG_n01_0722_CD8_low_d2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2K2WBGXG_n01_0722_CD8_low_d2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14606058
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACTGTATCTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCACTAT1268477886.84600595177699No Hit
ATACTGTATCTCTTGTGGAAAGGACGAAACACCGCGAGTAGGTCCACTAT5958804.079677076456906No Hit
ATACTGTATCTCTTGTGGAAAGGACGAAACACCGCCACTAGGTCCACTAT1931871.3226498210537025No Hit
ATACTGTATCTCTTGTGGAAAGGAAGAAACACCGCCAGTAGGTCCACTAT655340.4486768435398518No Hit
ATACTGTATCTCTTTTGGAAAGGACGAAACACCGCCAGTAGGTCCACTAT534360.3658481980559026No Hit
ATACTGTATCTCTTGTGGAAAGGACGAAACACCACCAGTAGGTCCACTAT384740.2634112503181899No Hit
ATACTGTATCTCTTGTGGAAAGGACGAAACACCGCCAGGAGGTCCACTAT290310.1987599939696255No Hit
ATACTGTATCTCTTGTGGAAAGGACGAAACACCTCCAGTAGGTCCACTAT271880.18614194192574068No Hit
ATACTGTATCTCTTGTGGAAAAGACGAAACACCGCCAGTAGGTCCACTAT210750.14428944483172668No Hit
ATACTGTATCTCTTGTGGAAAGGACAAAACACCGCCAGTAGGTCCACTAT205130.14044172630288063No Hit
ATACTGTATCTCTTGTGGAAAGAACGAAACACCGCCAGTAGGTCCACTAT203840.1395585311245512No Hit
ATACTGTATCTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCACTATG194700.1333008536594884No Hit
ATACTGTATCTCTTGTGGAAAGGACGAAACACCGCCTGTAGGTCCACTAT189400.12967222230666206No Hit
ATACTGTATCTCTTGTAGAAAGGACGAAACACCGCCAGTAGGTCCACTAT177620.12160707563943673No Hit
ATACTGTATCTCTTGTGAAAAGGACGAAACACCGCCAGTAGGTCCACTAT168380.11528093343186778No Hit
ATACTGTATCTCTTATGGAAAGGACGAAACACCGCCAGTAGGTCCACTAT164580.11267927321663382No Hit
ATACTGTATCTCTTGTGGAAAGGAAGAAAAACCGCCAGTAGGTCCACTAT160530.10990645114513445No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACTGT14513500.0144.933491
CTGTATC14518700.0144.890984
ACTGTAT14520050.0144.8863
ATCTCTT14534900.0144.869728
TACTGTA14523850.0144.851612
TGTATCT14530250.0144.817725
TCTCTTG14446600.0144.746529
GTATCTC14538900.0144.723076
TATCTCT14563100.0144.662867
GTATGCC415150.0127.500175145
TGAACTC14103900.0125.90665145
TCTCTTT129750.095.159959
TGAACAC50500.093.60299145
TAACTCC34600.086.11905145
ACTATAT27750.085.705713
ATATCTC29150.081.342416
TATGCCG49150.081.275856145
TGAACCC42100.081.10961145
TGAACTA41000.071.615105145
TGACTCC27350.066.80003145
Produced by FastQC (version 0.11.5)