FastQCFastQC Report
Mon 27 Jul 2020
H2K2WBGXG_n01_0722_CD8_low_d1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2K2WBGXG_n01_0722_CD8_low_d1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11762509
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAACAATGGTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCACTATG666862456.69389073368616No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACACCGCCTGTAGGTCCACTATG346728129.47739296097457No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACACCGCCACTAGGTCCACTATG6053785.146674064181375No Hit
TAACAATGGTCTTGTGGAAAGGAAGAAACACCGCCAGTAGGTCCACTATG543760.4622823242898263No Hit
TAACAATGGTCTTTTGGAAAGGACGAAACACCGCCAGTAGGTCCACTATG502860.42751083123507067No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACACCGCCTCTAGGTCCACTATG447330.3803015156035162No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACACCGCCAGGAGGTCCACTATG244720.20805085037554488No Hit
TAACAATGGTCTTGTGGAAAGGAAGAAACACCGCCTGTAGGTCCACTATG233800.19876711677755146No Hit
TAACAATGGTCTTTTGGAAAGGACGAAACACCGCCTGTAGGTCCACTATG201630.17141750964866426No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACACCACCAGTAGGTCCACTATG188690.16041645536679291No Hit
TAACAATGGTCTTGTGGAAAAGACGAAACACCGCCAGTAGGTCCACTATG130650.11107324126170699No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACACCGCCTCTACGTCCACTATG128690.10940693010309281No Hit
TAACAATGGTCTTGTGGAAAGGACAAAACACCGCCAGTAGGTCCACTATG127470.10836973642273089No Hit
TAACAATGGTCTTGTGGAAAGGAAGAAAAAAAGAAACTAGGTCCACTATG126100.10720501892920974No Hit
TAACAATGGTCTTGTGGAAAGAACGAAACACCGCCAGTAGGTCCACTATG125120.10637186334990265No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACACCTCCAGTAGGTCCACTATG123690.1051561363311178No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACAATG11697450.0144.930372
CAATGGT11672850.0144.910284
AATGGTC11681450.0144.900445
ACAATGG11673450.0144.898533
ATGGTCT11683800.0144.853276
TAACAAT11718950.0144.766651
TGGTCTT11682400.0144.735377
GGTCTTG11568400.0144.728848
GTCTTGT11592350.0144.521389
TCTCGTT160850.0129.62836145
GGTCTTT115050.0125.543438
ATGCCGT245400.0122.04298145
AACTCCA11400250.0121.05164145
CAATGAT26350.0112.274694
ATGCCGG38600.0101.611336145
GTCTTTT137350.0101.297529
AATGATC32100.091.9376755
GGTCTCG9900.082.762678
AATAGTC25100.081.1777045
GATCTTG36000.080.7668468
Produced by FastQC (version 0.11.5)