FastQCFastQC Report
Mon 27 Jul 2020
H2K2WBGXG_n01_0722_CD8_high_d3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2K2WBGXG_n01_0722_CD8_high_d3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11566547
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAATCACCGCCAGTAGGT771454666.69705314818674No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGCCAGTAGGT276438923.899863978419834No Hit
ACGATCTATAGGTAAGGTCTTGTGGAAAGGACGAATCACCGCCAGTAGGT1358031.1741014842199664No Hit
ACGATCTATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGCCAGTAGGT1141490.9868891727150721No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAATCACCTCCAGTAGGT205090.17731307364246218No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAAGACGAATCACCGCCAGTAGGT189490.1638259024063102No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAATCACCACCAGTAGGT180460.15601890520999914No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACAAATCACCGCCAGTAGGT171560.1483243011073227No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGAACGAATCACCGCCAGTAGGT170430.1473473457549604No Hit
ACGATCGATAGGTAAGATCTTGTGGAAAGGACGAATCACCGCCAGTAGGT163320.14120030809540654No Hit
ACGATCGATAGGTAAGGTCTTATGGAAAGGACGAATCACCGCCAGTAGGT161130.13930691674879286No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGAAGAAACACCGCCAGTAGGT155010.13401579572537942No Hit
ACGATCGATAGGTAAGGTCTTGTAGAAAGGACGAATCACCGCCAGTAGGT154320.1334192477668573No Hit
ACGATCGATAGGTAAGGTCTTGTGAAAAGGACGAATCACCGCCAGTAGGT149800.12951142635740814No Hit
ACGATCGATAGATAAGGTCTTGTGGAAAGGACGAATCACCGCCAGTAGGT144960.12532694502516611No Hit
ACGATCGATAGGTAAAGTCTTGTGGAAAGGACGAATCACCGCCAGTAGGT119800.10357455859557739No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGATCG11211700.0144.86541
CGATCGA11221300.0144.855622
ATCGATA11238300.0144.829974
CGATAGG11199700.0144.809866
GATAGGT11198800.0144.795147
GATCGAT11231950.0144.758273
ATAGGTA11489050.0144.744498
TCGATAG11231900.0144.677475
TAGGTAA11488650.0144.668819
ACGATCT293350.0139.59491
CTATAGG290450.0139.35086
GATCTAT295600.0138.269123
CGATCTA296900.0137.931752
TATAGGT297550.0135.70427
ATCTATA302000.0135.269234
TCTATAG303300.0134.211885
ACGTCTG11163700.0131.47153145
ATCTCGT100500.0116.14288145
CGATAGA29500.099.054466
GATAGAT29950.096.8365257
Produced by FastQC (version 0.11.5)