FastQCFastQC Report
Mon 27 Jul 2020
H2K2WBGXG_n01_0722_CD8_high_d2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2K2WBGXG_n01_0722_CD8_high_d2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10576005
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTC916594086.66731908693312No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACTCCGCCAGTAGGTC5143824.863670166570459No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAAGACCGCCAGTAGGTC1335911.2631518233964527No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCTCCAGTAGGTC247930.2344268937089194No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGAAGAAACACCGCCAGTAGGTC233440.22072606811362133No Hit
CGATCGATACAGGTATTCTTGTGGAAAAGACGAAACACCGCCAGTAGGTC223290.2111288714405865No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCACCAGTAGGTC218760.20684559056089705No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACAAAACACCGCCAGTAGGTC205130.19395792645710738No Hit
CGATCGATACAGGTATTCTTGTGGAAAGAACGAAACACCGCCAGTAGGTC199320.1884643587063357No Hit
CGATCGATACAGGTATTCTTGTAGAAAGGACGAAACACCGCCAGTAGGTC177730.16805022312300344No Hit
CGATCGATACAGGTATTCTTATGGAAAGGACGAAACACCGCCAGTAGGTC177600.16792730336265915No Hit
CGATCGATACAGGTATTCTTGTGAAAAGGACGAAACACCGCCAGTAGGTC168000.15885015182954243No Hit
CGATCGATACAGATATTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTC158800.15015121494363892No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGCAGTAGGTCC128950.12192694689535416No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCCCCAGTAGGTC124430.11765312138184503No Hit
CGATCGATACAGGTATTCTTGTCGAAAGGACGAAACACCGCCAGTAGGTC110260.10425486750431756No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACAGGT10484800.0144.904058
ATACAGG10488950.0144.896447
CGATACA10516250.0144.889855
CGATCGA10508800.0144.878831
ATCGATA10526250.0144.87073
TCGATAC10523650.0144.832064
ACAGGTA10490250.0144.808849
GATCGAT10517350.0144.802322
GATACAG10520300.0144.687266
CGTCTGA10187450.0129.47783145
TCTCGTA183750.0117.85298145
ATACAGA28050.0102.882737
CGACTGA47900.096.41323145
GTCTGAA1124600.087.25552145
CTCTGAA29150.083.06931145
TACAGAT34850.082.597258
CGTCTAA38450.079.19279145
CAATCGA20100.078.635341
ACAGATA36250.078.401279
CGTTGAA32350.076.42101145
Produced by FastQC (version 0.11.5)