FastQCFastQC Report
Mon 27 Jul 2020
H2K2WBGXG_n01_0722_CD8_high_d1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2K2WBGXG_n01_0722_CD8_high_d1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9966509
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACTCCGCCAGTAGGTCC705025370.73944347012579No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCC224913122.56688876717013No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACTCCTCCAGTAGGTCC179220.17982224267293592No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACTCCACCAGTAGGTCC153440.15395561274263636No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACTCCGCCAGTAGGTCA136170.13662757942625647No Hit
GATCGATAACGCATTTCTTGTGGAAAGGAAGAAAAACCGCCAGTAGGTCC128380.12881140226733354No Hit
GATCGATAACGCATTTCTTGTGGAAAGGAAGAAACTCCGCCAGTAGGTCC127310.12773780668838006No Hit
GATCGATAACGCATTTCTTGTGGAAAAGACGAAACTCCGCCAGTAGGTCC117380.11777443837155015No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACAAAACTCCGCCAGTAGGTCC116740.1171322877448864No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACTCCCCCAGTAGGTCC114540.11492489496572973No Hit
GATCGATAACGCATTTCTTGTGGAAAGAACGAAACTCCGCCAGTAGGTCC109810.11017900049054288No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATAAC9914450.0144.844254
ATAACGC9917850.0144.831886
GATAACG9905050.0144.82875
AACGCAT9914750.0144.823878
TCGATAA9917400.0144.803383
ACGCATT9914650.0144.79549
TAACGCA9920900.0144.747217
ATCGATA9938200.0144.53972
GAGCACA9643350.0131.13171145
AGCACAC930700.0110.061035145
TTACCTA87200.0104.17572145
GAGCACC29250.096.91317145
GAGCCAC15150.087.57303145
AACACAT20400.079.968538
GATCGAT19639200.072.994341
GATCAAT23250.072.661451
ATCAATA23700.071.285742
AATAACG23650.071.131295
CAATAAC24100.070.103284
ACACATT23350.069.5510949