Basic Statistics
Measure | Value |
---|---|
Filename | H2K2WBGXG_n01_0722_CD8_high_d1.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 9966509 |
Sequences flagged as poor quality | 0 |
Sequence length | 151 |
%GC | 48 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACTCCGCCAGTAGGTCC | 7050253 | 70.73944347012579 | No Hit |
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCC | 2249131 | 22.56688876717013 | No Hit |
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACTCCTCCAGTAGGTCC | 17922 | 0.17982224267293592 | No Hit |
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACTCCACCAGTAGGTCC | 15344 | 0.15395561274263636 | No Hit |
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACTCCGCCAGTAGGTCA | 13617 | 0.13662757942625647 | No Hit |
GATCGATAACGCATTTCTTGTGGAAAGGAAGAAAAACCGCCAGTAGGTCC | 12838 | 0.12881140226733354 | No Hit |
GATCGATAACGCATTTCTTGTGGAAAGGAAGAAACTCCGCCAGTAGGTCC | 12731 | 0.12773780668838006 | No Hit |
GATCGATAACGCATTTCTTGTGGAAAAGACGAAACTCCGCCAGTAGGTCC | 11738 | 0.11777443837155015 | No Hit |
GATCGATAACGCATTTCTTGTGGAAAGGACAAAACTCCGCCAGTAGGTCC | 11674 | 0.1171322877448864 | No Hit |
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACTCCCCCAGTAGGTCC | 11454 | 0.11492489496572973 | No Hit |
GATCGATAACGCATTTCTTGTGGAAAGAACGAAACTCCGCCAGTAGGTCC | 10981 | 0.11017900049054288 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
CGATAAC | 991445 | 0.0 | 144.84425 | 4 |
ATAACGC | 991785 | 0.0 | 144.83188 | 6 |
GATAACG | 990505 | 0.0 | 144.8287 | 5 |
AACGCAT | 991475 | 0.0 | 144.82387 | 8 |
TCGATAA | 991740 | 0.0 | 144.80338 | 3 |
ACGCATT | 991465 | 0.0 | 144.7954 | 9 |
TAACGCA | 992090 | 0.0 | 144.74721 | 7 |
ATCGATA | 993820 | 0.0 | 144.5397 | 2 |
GAGCACA | 964335 | 0.0 | 131.13171 | 145 |
AGCACAC | 93070 | 0.0 | 110.061035 | 145 |
TTACCTA | 8720 | 0.0 | 104.17572 | 145 |
GAGCACC | 2925 | 0.0 | 96.91317 | 145 |
GAGCCAC | 1515 | 0.0 | 87.57303 | 145 |
AACACAT | 2040 | 0.0 | 79.96853 | 8 |
GATCGAT | 1963920 | 0.0 | 72.99434 | 1 |
GATCAAT | 2325 | 0.0 | 72.66145 | 1 |
ATCAATA | 2370 | 0.0 | 71.28574 | 2 |
AATAACG | 2365 | 0.0 | 71.13129 | 5 |
CAATAAC | 2410 | 0.0 | 70.10328 | 4 |
ACACATT | 2335 | 0.0 | 69.551094 | 9 |