FastQCFastQC Report
Mon 27 Jul 2020
H2K2WBGXG_n01_0722_CD8_flowthrough.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2K2WBGXG_n01_0722_CD8_flowthrough.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7480273
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCA691945392.50268004924419No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACAGCGCCAGTAGGTCCA1321551.7667135945439425No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGAAGAAAAACCGCCAGTAGGTCCA153190.20479199088054675No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCAC114460.15301580570655643No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACACCACCAGTAGGTCCA107800.1441123873420128No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGAAGAAACACCGCCAGTAGGTCCA97500.1303428364178687No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACACCGCAGTAGGTCCAC87770.11733528976816754No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACACCCCCAGTAGGTCCA86570.11573107024302456No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACAAAACACCGCCAGTAGGTCCA79760.10662712443783803No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGATT7443150.0144.905141
CGATTCC7442500.0144.90023
TCGATTC7446500.0144.877872
TTCCTTG7447100.0144.85746
GATTCCT7448950.0144.84874
ATTCCTT7454450.0144.82455
CCTTGGT7442100.0144.776548
TCCTTGG7442500.0144.765757
CTTGGTT7443750.0144.629569
CACGTCT7198000.0131.69933145
ACGTCTG664200.0118.3007145
CACGCTG22050.0105.54399145
CACGTCG28750.0104.1476145
CACGTTG18050.090.37376145
CCTTAGT10200.076.77078
CCGTCTG11950.075.229965145
CACTCTG28300.075.061676145
CCTTGAT13850.071.72048
CACGACT20950.068.866196145
CGTCTGA123150.067.23087145