FastQCFastQC Report
Mon 27 Jul 2020
H2K2WBGXG_n01_0722_CD4_prestim.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2K2WBGXG_n01_0722_CD4_prestim.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7200328
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTC628139787.23765084035061No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACTCCGCCAGTAGGTC3549264.92930322063106No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAAGACCGCCAGTAGGTC996721.3842702721320475No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGAAGAAACACCGCCAGTAGGTC160610.22305928285489218No Hit
CGATCGATACAGGTATTCTTGTGGAAAGAACGAAACACCGCCAGTAGGTC117900.16374254061759408No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACAAAACACCGCCAGTAGGTC117550.16325645165053593No Hit
CGATCGATACAGGTATTCTTGTGGAAAAGACGAAACACCGCCAGTAGGTC116480.1617704082369581No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCACCAGTAGGTC110410.1533402367225493No Hit
CGATCGATACAGGTATTCTTGTGAAAAGGACGAAACACCGCCAGTAGGTC104080.1445489705468973No Hit
CGATCGATACAGGTATTCTTGTAGAAAGGACGAAACACCGCCAGTAGGTC102070.14175743105036326No Hit
CGATCGATACAGGTATTCTTATGGAAAGGACGAAACACCGCCAGTAGGTC100400.1394380922646857No Hit
CGATCGATACAGATATTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTC88590.12303606169052297No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGCAGTAGGTCC84190.11692522896179175No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCCCCAGTAGGTC84180.11691134070559007No Hit
CGATCGATACAGGTATTCTTGTCGAAAGGACGAAACACCGCCAGTAGGTC82800.11499476134976075No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATACA7163400.0144.847375
ATACAGG7150550.0144.840017
TCGATAC7165850.0144.836294
ACAGGTA7149850.0144.76919
TACAGGT7151700.0144.747828
GATACAG7165450.0144.708796
ATCGATA7191600.0144.356023
CGATCGA7193150.0144.17781
AGCACAC6995400.0132.97832145
GCACACG582500.0122.3462145
ATACAGA17800.093.280457
CACACGT92700.080.398186145
TACAGAT21900.075.48388
GATCGAT14226250.072.906012
ACAGATA23550.069.883869
ATATAGG10350.067.952677
AGCAACG11950.067.3423145
ACACACG15650.064.39221145
AGAACAC18850.063.07618145
AGCAGAC9500.062.57821145
Produced by FastQC (version 0.11.5)