FastQCFastQC Report
Mon 27 Jul 2020
H2K2WBGXG_n01_0722_CD4_presort_d2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2K2WBGXG_n01_0722_CD4_presort_d2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7658152
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAACAATGGTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCACTATG437093057.075519002495646No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACACCGCCTGTAGGTCCACTATG227224829.67097022884894No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACACCGCCACTAGGTCCACTATG3986775.205916518763273No Hit
TAACAATGGTCTTGTGGAAAGGAAGAAACACCGCCAGTAGGTCCACTATG354450.46284012121984525No Hit
TAACAATGGTCTTTTGGAAAGGACGAAACACCGCCAGTAGGTCCACTATG297280.3881876463146723No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACACCGCCTCTAGGTCCACTATG287650.3756128110280391No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACACCGCCAGGAGGTCCACTATG159870.20875793533479095No Hit
TAACAATGGTCTTGTGGAAAGGAAGAAACACCGCCTGTAGGTCCACTATG154940.2023203509149466No Hit
TAACAATGGTCTTTTGGAAAGGACGAAACACCGCCTGTAGGTCCACTATG119540.15609509970551644No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACACCACCAGTAGGTCCACTATG90760.11851423163186106No Hit
TAACAATGGTCTTGTGGAAAGGAAGAAAAAAAGAAACTAGGTCCACTATG81870.10690568690723298No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACACCGCCTCTACGTCCACTATG81500.10642254162623047No Hit
TAACAATGGTCTTGTGGAAAGAACGAAACACCGCCAGTAGGTCCACTATG77740.10151274093279944No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACAATG7623350.0144.874442
ACAATGG7612700.0144.832323
GGTCTTG7542850.0144.79328
ATGGTCT7615850.0144.788576
TAACAAT7631300.0144.785311
CAATGGT7614250.0144.7794
AATGGTC7617750.0144.768635
TGGTCTT7610750.0144.726527
GTCTTGT7558200.0144.529029
ACGTCTG7365650.0131.6837145
CGTCTGA680300.0122.9916145
GGTCTTT69250.0117.370248
GTCTTTT80050.097.815819
ACGTCTA29900.094.32154145
ACGACTG16800.091.918465145
ACGTCGA25000.084.678925145
ACGCTGA19250.084.362564145
ACTCTGA26300.083.52555145
ACGTCAG35000.082.234665145
CAATGAT18550.080.9130254
Produced by FastQC (version 0.11.5)