FastQCFastQC Report
Mon 27 Jul 2020
H2K2WBGXG_n01_0722_CD4_presort_d1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2K2WBGXG_n01_0722_CD4_presort_d1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7038358
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAATCACCGCCAGTAGGT476256067.66578227478625No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGCCAGTAGGT166152623.606727591861624No Hit
ACGATCTATAGGTAAGGTCTTGTGGAAAGGACGAATCACCGCCAGTAGGT826701.1745637263691333No Hit
ACGATCTATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGCCAGTAGGT685670.9741902869959159No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAAGACGAATCACCGCCAGTAGGT95000.13497466312455264No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGAAGAAACACCGCCAGTAGGT93230.13245987203265308No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGAACGAATCACCGCCAGTAGGT92800.13184893408377352No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACAAATCACCGCCAGTAGGT90680.12883686791720456No Hit
ACGATCGATAGGTAAGGTCTTGTGAAAAGGACGAATCACCGCCAGTAGGT86320.1226422412727514No Hit
ACGATCGATAGGTAAGGTCTTGTAGAAAGGACGAATCACCGCCAGTAGGT85440.12139194965643976No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAATCACCACCAGTAGGT85370.12129249464150588No Hit
ACGATCGATAGGTAAGGTCTTATGGAAAGGACGAATCACCGCCAGTAGGT84420.11994274801026034No Hit
ACGATCGATAGGTAAGATCTTGTGGAAAGGACGAATCACCGCCAGTAGGT83390.11847933850480467No Hit
ACGATCGATAGATAAGGTCTTGTGGAAAGGACGAATCACCGCCAGTAGGT76060.10806497765529972No Hit
ACGATCGATAGGTAAAGTCTTGTGGAAAGGACGAATCACCGCCAGTAGGT72240.10263757541176506No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATAGG6812400.0144.884316
GATAGGT6812000.0144.868687
ATAGGTA6990200.0144.769978
ATCGATA6840050.0144.745074
TCGATAG6834650.0144.651415
TAGGTAA6992000.0144.649819
ACGATCG6833950.0144.619051
CTATAGG174700.0140.135256
GATCTAT178550.0138.323153
ACGATCT179450.0137.943861
ATCTATA180900.0136.45194
CGATCTA183950.0134.5762
AGAGCAC6840000.0134.51878145
TCTATAG183100.0134.339055
TATAGGT182150.0134.155267
GAGCACA494300.0120.59236145
AGAGCAA34000.0109.8149145
ACAATCG12500.084.108581
GATAAGT16850.080.903057
ATAGATA20950.079.949628
Produced by FastQC (version 0.11.5)