FastQCFastQC Report
Mon 27 Jul 2020
H2K2WBGXG_n01_0722_CD4_low_d2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2K2WBGXG_n01_0722_CD4_low_d2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6622991
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACTCCGCCAGTAGGTCC469515370.89173154546036No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCC147824122.31984008433652No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACTCCACCAGTAGGTCC112780.17028560056928962No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACTCCTCCAGTAGGTCC101070.15260476724187003No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACTCCGCCAGTAGGTCA99410.1500983468043366No Hit
GATCGATAACGCATTTCTTGTGGAAAGGAAGAAACTCCGCCAGTAGGTCC85200.12864278390231845No Hit
GATCGATAACGCATTTCTTGTGGAAAGGAAGAAAAACCGCCAGTAGGTCC84610.1277519477227132No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACAAAACTCCGCCAGTAGGTCC75130.11343817317583552No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACTCCCCCAGTAGGTCC74700.11278891968900456No Hit
GATCGATAACGCATTTCTTGTGGAAAGAACGAAACTCCGCCAGTAGGTCC73970.11168669865322178No Hit
GATCGATAACGCATTTCTTGTGGAAAAGACGAAACTCCGCCAGTAGGTCC69940.10560183457896893No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACTCCGCAGTAGGTCCA68790.10386545897465359No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATAAC6580850.0144.912544
ATAACGC6583650.0144.880636
ATCGATA6589600.0144.819152
GATAACG6579950.0144.813345
TCGATAA6586000.0144.801443
TAACGCA6586650.0144.775057
ACGCATT6584250.0144.746439
AACGCAT6587200.0144.740988
GATCGAT6580550.0144.713171
ACGTCTG6366950.0134.41145
CGTCTGA468650.0112.62027145
ACGACTG23600.0111.20656145
ACGTCGA21550.094.53506145
ATCTCGT26950.086.891556145
ACGTCTA21400.085.71179145
ACGCTGA10300.076.722565145
AATCGAT14700.071.518311
ACTCTGA21300.070.11669145
TCGATAC15950.070.007783
GATACGC4450.068.435615
Produced by FastQC (version 0.11.5)