FastQCFastQC Report
Mon 27 Jul 2020
H2K2WBGXG_n01_0722_CD4_low_d1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2K2WBGXG_n01_0722_CD4_low_d1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7420477
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCA681268591.80925970122945No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACAGCGCCAGTAGGTCCA1366981.8421726797347395No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACACCTCCAGTAGGTCCA198720.2677994959084167No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGAAGAAAAACCGCCAGTAGGTCCA146160.19696846981669774No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACACCACCAGTAGGTCCA139200.1875890188730455No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCAC108000.14554320429805254No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGAAGAAACACCGCCAGTAGGTCCA95960.12931783226334373No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACACCGCAGTAGGTCCAC93350.12580053815947412No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACACCCCCAGTAGGTCCA86390.11642108721582184No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACAAAACACCGCCAGTAGGTCCA79290.10685296915548691No Hit
ATCGATTCCTTGGTTCTTGTGGAAAAGACGAAACACCGCCAGTAGGTCCA77310.10418467707668927No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATTCC7379000.0144.901413
TCGATTC7382950.0144.90052
ATCGATT7382250.0144.85831
ATTCCTT7390550.0144.83685
CCTTGGT7375650.0144.786368
TCCTTGG7376650.0144.773597
TTCCTTG7388650.0144.759236
CTTGGTT7401600.0144.168159
GATTCCT7504400.0142.56124
GTCTGAA7154950.0129.50494145
CTCGTAT92800.0122.10755145
GACTGAA31650.0107.20219145
TCTGAAC769350.090.26674145
CTATTCC14200.079.148943
CCTTGTT12050.076.4189158
GTCGAAC22450.076.21267145
GACAGAA9150.072.102745145
GCTGAAC16400.072.056854145
GTGTATG46950.071.4952145
CCTTAGT11300.066.09118