FastQCFastQC Report
Mon 27 Jul 2020
H2K2WBGXG_n01_0722_CD4_high_d2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2K2WBGXG_n01_0722_CD4_high_d2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7506273
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATAGGTCGCATCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCACTA674035489.79628105719043No Hit
GATAGGTCGCATCTTGTGGAAAGGACGAAACACCGGCAGTAGGTCCACTA1384541.844510584680307No Hit
GATAGGTCGCATCTTGTGGAAAGGAAGAAACACCGCCAGTAGGTCCACTA325340.43342415070701534No Hit
GATAGGTCGCATCTTGAGGAAAGGACGAAACACCGCCAGTAGGTCCACTA250050.33312137728004293No Hit
GATAGGTCGCATCTTGTGGAAAGGACGAAACACCACCAGTAGGTCCACTA174070.23189937269800873No Hit
GATAGGTCGCATCTTGTGGAAAGGACGAAACACCTCCAGTAGGTCCACTA164020.21851057109167227No Hit
GATAGGTCGCATCTTGTGGAAAAGACGAAACACCGCCAGTAGGTCCACTA160520.2138478043630974No Hit
GATAGGTCGCATCTTGTGGAAAGGACGAAACACCGCCAGGAGGTCCACTA154150.2053615689170911No Hit
GATAGGTCGCATCTTGTGGAAAGGACAAAACACCGCCAGTAGGTCCACTA146710.19544985907120616No Hit
GATAGGTCGCATCTTGTGGAAAGAACGAAACACCGCCAGTAGGTCCACTA146310.19491697144508333No Hit
GATAGGTCGCATCTTATGGAAAGGACGAAACACCGCCAGTAGGTCCACTA130800.17425425374217007No Hit
GATAGGTCGCATCTTGTAGAAAGGACGAAACACCGCCAGTAGGTCCACTA127810.17027091873690178No Hit
GATAGGTCGCATCTTGTGAAAAGGACGAAACACCGCCAGTAGGTCCACTA125850.16765976936889984No Hit
GATAGGTCGCATCTTGTGGAAAGGAAGAAAAAAAGCCAGTAGGTCCACTA112530.14991461141900914No Hit
GATAGGTCGCATCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCACTAT106980.14252079560655467No Hit
GATAGATCGCATCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCACTA102730.13685886457899946No Hit
GATAAGTCGCATCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCACTA100720.13418110425773216No Hit
GATAGGTCGCATCTTGTGGAAAGGACGAAACACCGCAGTAGGTCCACTAT93300.12429603879315342No Hit
GATAGGTCACATCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCACTA91660.12211119952604975No Hit
GATAGGTCGCATCTTGTGGAAAGGACGAAACACCGCCACTAGGTCCACTA91340.12168488942515146No Hit
AATAGGTCGCATCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCACTA83410.11112039223726608No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGTCGC7443600.0144.936544
TCGCATC7454650.0144.890987
GATAGGT7447250.0144.890871
CGCATCT7454900.0144.874518
TAGGTCG7446950.0144.85383
GCATCTT7453900.0144.802559
ATAGGTC7467000.0144.730962
GGTCGCA7453300.0144.707055
GTCGCAT7458450.0144.682976
GAACTCC7246900.0128.1521145
TATGCCG81000.0113.40281145
AAGTCGC14900.094.893934
ATCGCAT16750.093.937276
GAACTCA40200.092.87813145
CGCATCC7650.091.9317868
GATAGAT18350.086.532811
ATCTCGT249050.083.57548145
AACTCCA854000.082.482506145
GATAAGT17250.082.383411
GAACCCA18850.080.76816145
Produced by FastQC (version 0.11.5)