FastQCFastQC Report
Mon 27 Jul 2020
H2K2WBGXG_n01_0722_CD4_high_d1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2K2WBGXG_n01_0722_CD4_high_d1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8358110
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACTGTATCTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCCACTAT726403486.910007166692No Hit
ATACTGTATCTCTTGTGGAAAGGACGAAACACCGCGAGTAGGTCCACTAT3445054.121805049227636No Hit
ATACTGTATCTCTTGTGGAAAGGACGAAACACCGCCACTAGGTCCACTAT1117661.3372161888273786No Hit
ATACTGTATCTCTTGTGGAAAGGAAGAAACACCGCCAGTAGGTCCACTAT377380.4515135598837536No Hit
ATACTGTATCTCTTTTGGAAAGGACGAAACACCGCCAGTAGGTCCACTAT287690.3442046108510178No Hit
ATACTGTATCTCTTGTGGAAAGGACGAAACACCACCAGTAGGTCCACTAT205770.24619202188054476No Hit
ATACTGTATCTCTTGTGGAAAGGACGAAACACCGCCAGGAGGTCCACTAT168660.20179203193066375No Hit
ATACTGTATCTCTTGTGGAAAGGACGAAACACCTCCAGTAGGTCCACTAT157500.18843973099181513No Hit
ATACTGTATCTCTTGTGGAAAGAACGAAACACCGCCAGTAGGTCCACTAT116160.13897878826672538No Hit
ATACTGTATCTCTTGTGGAAAAGACGAAACACCGCCAGTAGGTCCACTAT115890.13865574872788225No Hit
ATACTGTATCTCTTGTGGAAAGGACAAAACACCGCCAGTAGGTCCACTAT114990.1375789502650719No Hit
ATACTGTATCTCTTGTGGAAAGGACGAAACACCGCCAGTAGGTCACTATG111230.13308032557599744No Hit
ATACTGTATCTCTTGTGGAAAGGACGAAACACCGCCTGTAGGTCCACTAT102240.12232430537525829No Hit
ATACTGTATCTCTTGTAGAAAGGACGAAACACCGCCAGTAGGTCCACTAT98220.11751460557470528No Hit
ATACTGTATCTCTTGTGAAAAGGACGAAACACCGCCAGTAGGTCCACTAT94620.11320741172346381No Hit
ATACTGTATCTCTTATGGAAAGGACGAAACACCGCCAGTAGGTCCACTAT94290.11281258562043334No Hit
ATACTGTATCTCTTGTGGAAAGGAAGAAAAACCGCCAGTAGGTCCACTAT90790.10862503604283744No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACTGT8306200.0144.934251
CTGTATC8308800.0144.883564
ACTGTAT8312450.0144.82783
TACTGTA8315200.0144.778182
ATCTCTT8324000.0144.764548
TGTATCT8322900.0144.704335
TATCTCT8331950.0144.689917
GTATCTC8323500.0144.688676
TCTCTTG8277450.0144.637169
AACTCCA8068400.0125.807594145
ATGCCGT115100.0122.82632145
TCTCTTT62700.0103.836199
TCTCGTA368600.084.57563145
AACTCAG65500.083.23551145
ACTCCAG1085300.078.25058145
CTATATC15200.074.419364
AACTCCG15500.072.96675145
ACTATAT15650.072.279063
ATACTAT16150.070.035461
AACTCAA24150.069.947296145
Produced by FastQC (version 0.11.5)