FastQCFastQC Report
Tue 29 May 2018
H2JY7AFXY_n02_ds774-041-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2JY7AFXY_n02_ds774-041-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12216178
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3816593.124209552283865No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC1779541.4567076543907596Illumina Single End PCR Primer 1 (100% over 50bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACGA70600.050.31682619
AAAGGGG252200.049.88624666
ACACGAG72200.049.10502620
CACGAGA75600.046.801921
GAGCACA272250.046.63921716
CACGTAG61100.046.6248421
AGAGCAC279100.046.6227515
GCACACG205100.045.1007118
AAGGGGG284250.044.433867
AGCACAC223600.044.03011317
ACACGTA63900.043.87178820
AGGGGGG311550.041.1617568
AAAAGGG312300.040.52130565
AAGAGCA406050.038.14874314
CACACGT151600.037.1226319
CACGCGT7500.035.46534719
GCACAAG71200.035.29363318
GTGACTG52800.035.017761
ACGAGAT101450.034.9800222
ACGTCAG67500.034.73802622
Produced by FastQC (version 0.11.2)