FastQCFastQC Report
Tue 29 May 2018
H2JY7AFXY_n02_ds774-041-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2JY7AFXY_n02_ds774-041-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13040838
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC4906383.7623195687271016Illumina Single End PCR Primer 1 (100% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3749462.8751679915048403No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG249750.062.37407718
GAGCACA397250.061.97986216
AGAGCAC406900.061.68879715
AGCACAC278350.061.19587717
ACACGAG111100.059.66568820
CACACGA111500.059.44981819
CACACGT152450.057.64540519
AAAGGGG619050.057.40740666
CACGTAG86800.056.68950321
CACGAGA116950.056.1396521
ACACGTA88300.055.45181320
AAGGGGG644450.055.0796267
AAGAGCA594250.052.83564814
AAAAGGG694550.051.3536565
GCACAAG134200.051.2462518
AGGGGGG705450.050.6753468
AGCACAA143600.048.06229817
ACACGTC79950.047.8476320
GAGCAAG114600.046.9701716
ACGTCAG71500.046.9407122
Produced by FastQC (version 0.11.2)