FastQCFastQC Report
Tue 29 May 2018
H2JY7AFXY_n02_ds774-032-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2JY7AFXY_n02_ds774-032-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17494
Sequences flagged as poor quality0
Sequence length76
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC420324.025380130330397Illumina Single End PCR Primer 1 (100% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5413.0924888533211385No Hit
AAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGC590.33725848862467134Illumina Single End PCR Primer 1 (100% over 49bp)
TAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGC530.3029610152052132Illumina Single End PCR Primer 1 (100% over 49bp)
GAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGC350.20006859494683893Illumina Single End PCR Primer 1 (100% over 49bp)
ACACTCTTTCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTC340.19435234937692925Illumina Single End PCR Primer 1 (100% over 40bp)
ACACTCTTTCCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCT310.1772036126672002Illumina Single End PCR Primer 1 (100% over 39bp)
AGGTCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC290.16577112152738083Illumina Single End PCR Primer 1 (98% over 50bp)
AGATAGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC270.15433863038756143Illumina Single End PCR Primer 1 (98% over 50bp)
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG260.14862238481765175No Hit
ACACTCTTTCCCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATC240.1371898936778324Illumina Single End PCR Primer 1 (100% over 38bp)
GTGACTGGAGTTCAGACGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG180.1028924202583743Illumina Single End PCR Primer 1 (100% over 32bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACTCT252.6797024E-670.198271
GTGACTG207.604293E-570.198271
CGCGCGT353.43789E-969.9971419
GGAGAAG150.002188622569.997132
CACACGG150.002188622569.9971319
CACACGA800.069.9971319
GGGCAGA309.666837E-869.997133
ACGTCTA207.7137694E-569.9971322
TGACTGG207.7137694E-569.997132
CACGCGT252.7260994E-669.9971319
CACAAGA500.069.9971319
CTCTTTC150.002188622569.997134
AGGGCAG150.002188622569.997132
ACTCTTT207.7137694E-569.997133
CCGCAGA252.7260994E-669.997133
GGGAGAT150.002188622569.997134
GAGCACG401.2187229E-1069.9971316
CACTCTT252.7260994E-669.997132
TTAAGAT150.002188622569.997134
GGCCAGA150.002188622569.997133
Produced by FastQC (version 0.11.2)