FastQCFastQC Report
Tue 29 May 2018
H2JY7AFXY_n02_ds774-032-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2JY7AFXY_n02_ds774-032-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11946344
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC128564810.76185316612346Illumina Single End PCR Primer 1 (100% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3404802.850076977525509No Hit
ACACTCTTTCCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCT249030.20845708109527064Illumina Single End PCR Primer 1 (100% over 39bp)
ACACTCTTTCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTC201900.1690056807337877Illumina Single End PCR Primer 1 (100% over 40bp)
AAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGC157110.13151303863340952Illumina Single End PCR Primer 1 (100% over 49bp)
ACACTCTTTCCCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATC149830.12541912404330563Illumina Single End PCR Primer 1 (100% over 38bp)
GTGACTGGAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTC123120.10306081927659208Illumina Single End PCR Primer 1 (100% over 40bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA744800.064.4334416
CACACGA213400.064.3220319
AGAGCAC757200.064.2330915
ACACGAG214600.063.9794620
GCACACG471500.063.38313318
AGCACAC518200.063.2435217
CACGTAG156800.062.8535121
ACACGTA157150.062.6702920
CACGAGA222300.061.47867621
AAGAGCA999750.060.81120314
CACACGT266250.059.67780319
GCACAAG233950.059.64465718
AAAGGGG1541950.059.08317666
AAGGGGG1542250.059.00587567
AGCACAA238400.058.51713617
AGGGGGG1573650.057.94627868
GAGCAAG188550.057.72767316
ACACTCT118550.056.2401581
GATCGAG72600.056.215938
AAAAGGG1638550.055.74328665
Produced by FastQC (version 0.11.2)